Article ; Online: Bioinformatics analysis of epitope-based vaccine design against the novel SARS-CoV-2.
Infectious diseases of poverty
2020 Volume 9, Issue 1, Page(s) 88
Abstract: Background: An outbreak of infection caused by SARS-CoV-2 recently has brought a great challenge to public health. Rapid identification of immune epitopes would be an efficient way to screen the candidates for vaccine development at the time of pandemic. ...
Abstract | Background: An outbreak of infection caused by SARS-CoV-2 recently has brought a great challenge to public health. Rapid identification of immune epitopes would be an efficient way to screen the candidates for vaccine development at the time of pandemic. This study aimed to predict the protective epitopes with bioinformatics methods and resources for vaccine development. Methods: The genome sequence and protein sequences of SARS-CoV-2 were retrieved from the National Center for Biotechnology Information (NCBI) database. ABCpred and BepiPred servers were utilized for sequential B-cell epitope analysis. Discontinuous B-cell epitopes were predicted via DiscoTope 2.0 program. IEDB server was utilized for HLA-1 and HLA-2 binding peptides computation. Surface accessibility, antigenicity, and other important features of forecasted epitopes were characterized for immunogen potential evaluation. Results: A total of 63 sequential B-cell epitopes on spike protein were predicted and 4 peptides (Spike Conclusions: B-cell epitopes on spike protein and T-cell epitopes within nucleocapsid protein were identified and recommended for developing a protective vaccine against SARS-CoV-2. |
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MeSH term(s) | Amino Acid Sequence ; Betacoronavirus/genetics ; Betacoronavirus/immunology ; COVID-19 ; COVID-19 Vaccines ; Computational Biology/methods ; Coronavirus Infections/immunology ; Coronavirus Infections/prevention & control ; Coronavirus Infections/virology ; Drug Design ; Epitopes, B-Lymphocyte/chemistry ; Epitopes, B-Lymphocyte/immunology ; Epitopes, T-Lymphocyte/chemistry ; Epitopes, T-Lymphocyte/immunology ; Humans ; Immunogenicity, Vaccine/immunology ; Models, Molecular ; Pandemics/prevention & control ; Pneumonia, Viral/immunology ; Pneumonia, Viral/prevention & control ; Pneumonia, Viral/virology ; SARS-CoV-2 ; Sequence Alignment ; Sequence Analysis ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/immunology ; Viral Envelope Proteins/immunology ; Viral Vaccines/immunology |
Chemical Substances | COVID-19 Vaccines ; Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins ; Viral Vaccines ; spike protein, SARS-CoV-2 |
Keywords | covid19 |
Language | English |
Publishing date | 2020-07-10 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 2689396-4 |
ISSN | 2049-9957 ; 2049-9957 |
ISSN (online) | 2049-9957 |
ISSN | 2049-9957 |
DOI | 10.1186/s40249-020-00713-3 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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