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Article ; Online: Global distribution, cross-species transmission, and receptor binding of canine parvovirus-2: Risks and implications for humans.

Chen, Songbiao / Shang, Ke / Chen, Jian / Yu, Zuhua / Wei, Ying / He, Lei / Ding, Ke

2024 Volume 930, Page(s) 172307

Abstract: For canine parvovirus -2 (CPV-2), a zoonotic virus capable of cross-species transmission in animals, the amino acid changes of capsid protein VP2 are key factors when binding to other species' transferrin receptors (TfR). CPV-2 variants can spread from ... ...

Abstract	For canine parvovirus -2 (CPV-2), a zoonotic virus capable of cross-species transmission in animals, the amino acid changes of capsid protein VP2 are key factors when binding to other species' transferrin receptors (TfR). CPV-2 variants can spread from felines and canines, for example, to Carnivora, Artiodactyla, and Pholidota species, and CPV-2c variants are essential to spread from Carnivora to Artiodactyla and Pholidota species in particular. In our study, a CPV-2a variant maintained a relatively stable trend, and the proportion of CPV-2c gradually rose from 1980 to 2021. The VP2 amino acid sequence analysis showed that five amino acid mutations at 426E/D, 305H/D, and 297S may be necessary for the virus to bind to different host receptors. Meanwhile, receptor-binding loop regions and amino acid sites 87 L, 93 N, 232I, and 305Y were associated with CPV-2 cross-species transmission. The homology of TfRs in different hosts infected with CPV-2 ranged from 77.2 % to 99.0 %, and from pig to feline, canine, and humans was 80.7 %, 80.4 %, and 77.2 %, respectively. The amino acid residues of TfRs involved in the viral binding in those hosts are highly conserved, which suggests that CPV-2 may be capable of pig-to-human transmission. Our analysis of the origin, evolutionary trend, cross-species transmission dynamics, and genetic characteristics of CPV-2 when binding to host receptors provides a theoretical basis for further research on CPV-2's mechanism of cross-species transmission and for establishing an early warning and monitoring mechanism for the possible threat of CPV-2 to animal-human public security.
MeSH term(s)	Parvovirus, Canine/genetics ; Animals ; Dogs ; Humans ; Parvoviridae Infections/veterinary ; Parvoviridae Infections/transmission ; Cats ; Capsid Proteins/metabolism ; Capsid Proteins/genetics ; Zoonoses/virology ; Zoonoses/transmission ; Receptors, Transferrin/metabolism ; Receptors, Transferrin/genetics
Chemical Substances	Capsid Proteins ; Receptors, Transferrin
Language	English
Publishing date	2024-04-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.172307
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Article: Rapid construction of infectious clones for distinct Newcastle disease virus genotypes.

Yu, Zuhua / Zhang, Yuhao / Li, Zedian / Yu, Qingzhong / Jia, Yanyan / Yu, Chuan / Chen, Jian / Chen, Songbiao / He, Lei

Frontiers in veterinary science

2023 Volume 10, Page(s) 1178801

Abstract: The reverse genetics system of the Newcastle disease virus (NDV) has provided investigators with a powerful approach to understand viral molecular biology and vaccine development. It has been impressively improved with modified strategies since its first ...

Abstract	The reverse genetics system of the Newcastle disease virus (NDV) has provided investigators with a powerful approach to understand viral molecular biology and vaccine development. It has been impressively improved with modified strategies since its first report, but it still poses some challenges. Most noteworthy, the genome complexity and length made full-length error-free cDNA assembly the most challenging and time-consuming step of NDV rescue. In the present study, we report a rapid full-length NDV genome construction with only a two-step ligation-independent cloning (LIC) strategy, which could be applied to distinct genotypes. In this approach, the genome of NDV was divided into two segments, and the cDNA clones were generated by RT-PCR followed by LIC. Subsequently, the infectious NDVs were rescued by co-transfection of the full-length cDNA clones and supporting plasmids expressing the NP, P, and L proteins of NDV in BHK-21 cells. Compared with the conventional cloning approaches, the two-step cloning method drastically reduced the number of cloning steps and saved researchers a substantial amount of time for constructing NDV infectious clones, thus enabling a rapid rescue of different genotypes of NDVs in a matter of weeks. Therefore, this two-step LIC cloning strategy may have an application to the rapid development of NDV-vectored vaccines against emerging animal diseases and the generation of different genotypes of recombinant NDVs for cancer therapy.
Language	English
Publishing date	2023-05-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2023.1178801
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Article ; Online: Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene

He, Lei / Wang, Hairong / Yu, Zuhua / Liao, Chengshui / Ding, Ke / Zhang, Cai / Yu, Chuan / Zhang, Chunjie

BMC Biotechnol. 2022 Dec., v. 22, no. 1 p.38-38

2022

Abstract: BACKGROUND: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent ... ...

Abstract	BACKGROUND: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system for NDV ZM10 is an indispensable platform. RESULTS: A full-length cDNA clone of NDV ZM10 and three supporting plasmids were constructed using the ligation-independent cloning method. Recombinant NDV rZM10 was successfully rescued after these plasmids were co-transfected into BHK-21 cells. Besides, the recombinant virus rZM10-RFP encoding the red fluorescent protein was generated by inserting the RFP gene into the full-length clone of NDV between the P and M genes. These rescued viruses were genetically and biologically identical to the parental strain and showed similar growth kinetics. CONCLUSION: The recovery system of NDV ZM10 strain was established, and can be used as a foundation for research on the enterotropic mechanism and development of multivalent vaccines against viral diseases of livestock and poultry.
Keywords	Avian orthoavulavirus 1 ; Newcastle disease ; genes ; growth models ; plasmids ; red fluorescent protein ; reverse genetics ; vaccines ; viruses ; China
Language	English
Dates of publication	2022-12
Size	p. 38.
Publishing place	BioMed Central
Document type	Article ; Online
ZDB-ID	2052746-9
ISSN	1472-6750
ISSN	1472-6750
DOI	10.1186/s12896-022-00763-5
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Article: A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone.

Li, Yijia / Chen, Songbiao / Yu, Zuhua / Yao, Jie / Jia, Yanyan / Liao, Chengshui / Chen, Jian / Wei, Ying / Guo, Rongxian / He, Lei / Ding, Ke

Foods (Basel, Switzerland)

2024 Volume 13, Issue 4

Abstract: Zearalenone (ZEN) is considered one of the most serious mycotoxins contaminating grains and their by-products, causing significant economic losses in the feed and food industries. Biodegradation pathways are currently considered the most efficient ... ...

Abstract	Zearalenone (ZEN) is considered one of the most serious mycotoxins contaminating grains and their by-products, causing significant economic losses in the feed and food industries. Biodegradation pathways are currently considered the most efficient solution to remove ZEN contamination from foods. However, low degradation rates and vulnerability to environmental impacts limit the application of biodegradation pathways. Therefore, the main research objective of this article was to screen strains that can efficiently degrade ZEN and survive under harsh conditions. This study successfully isolated a new strain L9 which can efficiently degrade ZEN from 108 food ingredients. The results of sequence alignment showed that L9 is
Language	English
Publishing date	2024-02-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods13040530
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Article ; Online: Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene.

He, Lei / Wang, Hairong / Yu, Zuhua / Liao, Chengshui / Ding, Ke / Zhang, Cai / Yu, Chuan / Zhang, Chunjie

BMC biotechnology

2022 Volume 22, Issue 1, Page(s) 38

Abstract: Background: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent ... ...

Abstract	Background: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system for NDV ZM10 is an indispensable platform. Results: A full-length cDNA clone of NDV ZM10 and three supporting plasmids were constructed using the ligation-independent cloning method. Recombinant NDV rZM10 was successfully rescued after these plasmids were co-transfected into BHK-21 cells. Besides, the recombinant virus rZM10-RFP encoding the red fluorescent protein was generated by inserting the RFP gene into the full-length clone of NDV between the P and M genes. These rescued viruses were genetically and biologically identical to the parental strain and showed similar growth kinetics. Conclusion: The recovery system of NDV ZM10 strain was established, and can be used as a foundation for research on the enterotropic mechanism and development of multivalent vaccines against viral diseases of livestock and poultry.
MeSH term(s)	Animals ; Newcastle disease virus/genetics ; DNA, Complementary/genetics ; Chickens/genetics ; Newcastle Disease ; Vaccines, Combined
Chemical Substances	DNA, Complementary ; Vaccines, Combined
Language	English
Publishing date	2022-12-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2052746-9
ISSN	1472-6750 ; 1472-6750
ISSN (online)	1472-6750
ISSN	1472-6750
DOI	10.1186/s12896-022-00763-5
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Article ; Online: 5′-Nucleotidase is dispensable for the growth of Salmonella Typhimurium but inhibits the bactericidal activity of macrophage extracellular traps

Qian, Man / Xu, Ke / Zhang, Mengke / Niu, Junhui / Zhao, Tianxiang / Wang, Xiaoli / Jia, Yanyan / Li, Jing / Yu, Zuhua / He, Lei / Li, Yinju / Wu, Tingcai / Wei, Ying / Chen, Jian / Chen, Songbiao / Zhang, Chunjie / Liao, Chengshui

Arch Microbiol. 2023 Jan., v. 205, no. 1 p.20-20

2023

Abstract: Salmonella enterica serovar Typhimurium (S. Typhimurium) is a zoonotic pathogen that causes severe gastroenteritis. The 5′-nucleotidases of pathogens can dephosphorylate adenosine phosphates, boost adenosine levels and suppress the pro-inflammatory ... ...

Abstract	Salmonella enterica serovar Typhimurium (S. Typhimurium) is a zoonotic pathogen that causes severe gastroenteritis. The 5′-nucleotidases of pathogens can dephosphorylate adenosine phosphates, boost adenosine levels and suppress the pro-inflammatory immune response. In our previous study, an extracellular nuclease, 5′-nucleotidase, was identified in the extracellular proteins of S. Typhimurium. However, the nuclease activity and the function of the 5′-nucleotidase of S. Typhimurium have not been explored. In the present study, deletion of the 5′-nucleotidase gene is dispensable for S. Typhimurium growth, even under environmental stress. Fluorescence microscopy revealed that the 5′-nucleotidase mutant induced more macrophage extracellular traps (METs) than the wild type did. Furthermore, recombinant 5′-nucleotidase protein (r5Nuc) could degrade λDNA, and the nuclease activity of r5Nuc was optimum at 37 °C and pH 6.0–7.0. The Mg²⁺ enhanced the nuclease activity of r5Nuc, whereas Zn²⁺ inhibited it. Meanwhile, deletion of the 5′-nucleotidase gene increased the bactericidal activity of METs, and r5Nuc could degrade METs and inhibit the bactericidal activity of METs. In conclusion, S. Typhimurium growth was independent of 5′-nucleotidase, but the nuclease activity of 5′-nucleotidase assisted S. Typhimurium to evade macrophage-mediated extracellular killing through degrading METs.
Keywords	Salmonella Typhimurium ; adenosine ; antibacterial properties ; fluorescence microscopy ; gastroenteritis ; genes ; immune response ; macrophages ; mutants ; pH ; pathogens
Language	English
Dates of publication	2023-01
Size	p. 20.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-022-03353-3
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Article: A

Huang, Meixue / Guo, Jing / Jia, Yanyan / Liao, Chengshui / He, Lei / Li, Jing / Wei, Ying / Chen, Songbiao / Chen, Jian / Shang, Ke / Guo, Rongxian / Ding, Ke / Yu, Zuhua

Biology

2023 Volume 12, Issue 9

Abstract: As a class I carcinogen, aflatoxin can cause serious damage to various tissues and organs through oxidative stress injuries. The liver, as the target organ of ... ...

Abstract	As a class I carcinogen, aflatoxin can cause serious damage to various tissues and organs through oxidative stress injuries. The liver, as the target organ of AFB
Language	English
Publishing date	2023-08-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology12091195
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Article: Chloroquine Inhibition of Autophagy Enhanced the Anticancer Effects of

Yu, Zuhua / Zhao, Yingying / Ding, Ke / He, Lei / Liao, Chengshui / Li, Jing / Chen, Songbiao / Shang, Ke / Chen, Jian / Yu, Chuan / Zhang, Chunjie / Li, Yinju / Wang, Shaohui / Jia, Yanyan

Microorganisms

2023 Volume 11, Issue 2

Abstract: ... Listeria ... ...

Abstract	Listeria monocytogenes
Language	English
Publishing date	2023-02-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11020408
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Article ; Online: Research Note: Anti-inflammatory effects and antiviral activities of baicalein and chlorogenic acid against infectious bursal disease virus in embryonic eggs.

Li, Yinju / Yang, Danfang / Jia, Yanyan / He, Lei / Li, Jing / Yu, Chuan / Liao, Chengshui / Yu, Zuhua / Zhang, Chunjie

Poultry science

2021 Volume 100, Issue 4, Page(s) 100987

Abstract: The purpose of this study was to investigate if baicalein and chlorogenic acid could inhibit the inflammatory responses induced by and protect against infectious bursal disease virus (IBDV) in chicken embryonic eggs. Nine-day-old embryonated chicken eggs ...

Abstract	The purpose of this study was to investigate if baicalein and chlorogenic acid could inhibit the inflammatory responses induced by and protect against infectious bursal disease virus (IBDV) in chicken embryonic eggs. Nine-day-old embryonated chicken eggs were randomly divided into 3 groups of 50 eggs per group: 1) treatment with varying concentrations of baicalein, 2) treatment with varying concentrations of chlorogenic acid, or 3) left untreated as a control. Forty-eight hours after hatching, each group was inoculated with a very virulent IBDV isolate, and the survival of the embryo was monitored daily until the embryonic livers were collected 72 h after inoculation. After IBDV infection, the viral loads in the embryonic livers were evaluated using qRT-PCR, and the hepatic content of inflammatory mediators, such as histamine, interleukin 1β (IL-1β), tumor necrosis factor alpha (TNF-α), and nuclear factor-kappa B (NF-κB), were examined. Significant antiviral potential was demonstrated at concentrations of 108 and 215 μg/egg of baicalein and chlorogenic acid, respectively. We observed a concentration-dependent response in the antiviral properties of these chemicals. Treating the embryos with baicalein and chlorogenic acid significantly reduced histamine production. Moreover, pretreatment with baicalein and chlorogenic acid signiﬁcantly inhibited NF-κB activation, and this inhibited the subsequent production of the proinflammatory cytokines TNF-α and IL-1β in the context of IBDV infection. These findings suggest that baicalein and chlorogenic acid have anti-IBDV properties, and they may be useful in the prevention of inflammation-related diseases.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Birnaviridae Infections/drug therapy ; Birnaviridae Infections/prevention & control ; Birnaviridae Infections/veterinary ; Chick Embryo ; Chickens ; Chlorogenic Acid/pharmacology ; Flavanones/pharmacology ; Infectious bursal disease virus/drug effects ; Ovum/virology ; Poultry Diseases/drug therapy ; Poultry Diseases/prevention & control ; Random Allocation
Chemical Substances	Anti-Inflammatory Agents ; Antiviral Agents ; Flavanones ; Chlorogenic Acid (318ADP12RI) ; baicalein (49QAH60606)
Language	English
Publishing date	2021-01-14
Publishing country	England
Document type	Journal Article
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.1016/j.psj.2021.01.010
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Article: Probiotic Properties of Chicken-Derived Highly Adherent Lactic Acid Bacteria and Inhibition of Enteropathogenic Bacteria in Caco-2 Cells.

Wang, Pudi / Chen, Songbiao / Liao, Chengshui / Jia, Yanyan / Li, Jing / Shang, Ke / Chen, Jian / Cao, Pinghua / Li, Wang / Li, Yuanxiao / Yu, Zuhua / Ding, Ke

Microorganisms

2022 Volume 10, Issue 12

Abstract: Lactic acid bacteria (LAB) as probiotic candidates have various beneficial functions, such as regulating gut microbiota, inhibiting intestinal pathogens, and improving gut immunity. The colonization of the intestine is a prerequisite for probiotic ... ...

Abstract	Lactic acid bacteria (LAB) as probiotic candidates have various beneficial functions, such as regulating gut microbiota, inhibiting intestinal pathogens, and improving gut immunity. The colonization of the intestine is a prerequisite for probiotic function. Therefore, it is necessary to screen the highly adherent LAB. In this study, the cell surface properties, such as hydrophobicity, auto-aggregation, co-aggregation, and adhesion abilities of the six chicken-derived LAB to Caco-2 cells were investigated. All six strains showed different hydrophobicity (21.18-95.27%), auto-aggregation (13.61-30.17%), co-aggregation with
Language	English
Publishing date	2022-12-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10122515
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