LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 5 of total 5

Search options

  1. Article ; Online: Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq.

    Yuan, Zishuo / Chen, Junjie / Xu, Yanwen / Zhou, Zhentao / Cai, Pengfei / Wei, Xiaoyu / Zheng, Huiwen / Zhang, Jufang / Yuan, Yue / Liu, Chuanyu

    STAR protocols

    2024  Volume 5, Issue 1, Page(s) 102848

    Abstract: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying transcriptomics. Here, we present an optimized protocol for dissociating human scalp tissue and acquiring high-quality single-cell suspension for scRNA-seq to study transcriptomics of ...

    Abstract Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying transcriptomics. Here, we present an optimized protocol for dissociating human scalp tissue and acquiring high-quality single-cell suspension for scRNA-seq to study transcriptomics of human hair follicles. We describe steps for human scalp tissue cleaning, subcutaneous fat removal, mechanical mincing, and enzymatic digestion. We then detail procedures for cleaning, resuspending, a cell viability assay, and library construction.
    MeSH term(s) Humans ; Hair Follicle ; Scalp ; Single-Cell Gene Expression Analysis ; Sequence Analysis, RNA/methods ; Gene Expression Profiling/methods
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Journal Article
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2024.102848
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin G

    Zhou, Yuye / Jonsson, Alexander / Sticker, Drago / Zhou, Guojun / Yuan, Zishuo / Kutter, Jörg P. / Emmer, Åsa

    Anal Bioanal Chem. 2023 Mar., v. 415, no. 6 p.1173-1185

    2023  

    Abstract: Proteins, and more specifically glycoproteins, have been widely used as biomarkers, e.g., to monitor disease states. Bottom-up approaches based on mass spectrometry (MS) are techniques commonly utilized in glycoproteomics, involving protein digestion and ...

    Abstract Proteins, and more specifically glycoproteins, have been widely used as biomarkers, e.g., to monitor disease states. Bottom-up approaches based on mass spectrometry (MS) are techniques commonly utilized in glycoproteomics, involving protein digestion and glycopeptide enrichment. Here, a dual function polymeric thiol-ene-based microfluidic chip (TE microchip) was applied for the analysis of the proteins osteopontin (OPN) and immunoglobulin G (IgG), which have important roles in autoimmune diseases, in inflammatory diseases, and in coronavirus disease 2019 (COVID-19). TE microchips with larger internal surface features immobilized with trypsin were successfully utilized for OPN digestion, providing rapid and efficient digestion with a residence time of a few seconds. Furthermore, TE microchips surface-modified with ascorbic acid linker (TEA microchip) have been successfully utilized for IgG glycopeptide enrichment. To illustrate the use of the chips for more complex samples, they were applied to enrich IgG glycopeptides from human serum samples with antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The dual functional TE microchips could provide high throughput for online protein digestion and glycopeptide enrichment, showing great promise for future extended applications in proteomics and the study of related diseases.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; ascorbic acid ; biomarkers ; blood serum ; digestion ; glycopeptides ; glycoproteomics ; humans ; immunoglobulin G ; mass spectrometry ; microchip technology ; organ-on-a-chip ; osteopontin ; polymers ; trypsin
    Language English
    Dates of publication 2023-03
    Size p. 1173-1185.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ISSN 1618-2642
    DOI 10.1007/s00216-022-04498-2
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin G.

    Zhou, Yuye / Jönsson, Alexander / Sticker, Drago / Zhou, Guojun / Yuan, Zishuo / Kutter, Jörg P / Emmer, Åsa

    Analytical and bioanalytical chemistry

    2023  Volume 415, Issue 6, Page(s) 1173–1185

    Abstract: Proteins, and more specifically glycoproteins, have been widely used as biomarkers, e.g., to monitor disease states. Bottom-up approaches based on mass spectrometry (MS) are techniques commonly utilized in glycoproteomics, involving protein digestion and ...

    Abstract Proteins, and more specifically glycoproteins, have been widely used as biomarkers, e.g., to monitor disease states. Bottom-up approaches based on mass spectrometry (MS) are techniques commonly utilized in glycoproteomics, involving protein digestion and glycopeptide enrichment. Here, a dual function polymeric thiol-ene-based microfluidic chip (TE microchip) was applied for the analysis of the proteins osteopontin (OPN) and immunoglobulin G (IgG), which have important roles in autoimmune diseases, in inflammatory diseases, and in coronavirus disease 2019 (COVID-19). TE microchips with larger internal surface features immobilized with trypsin were successfully utilized for OPN digestion, providing rapid and efficient digestion with a residence time of a few seconds. Furthermore, TE microchips surface-modified with ascorbic acid linker (TEA microchip) have been successfully utilized for IgG glycopeptide enrichment. To illustrate the use of the chips for more complex samples, they were applied to enrich IgG glycopeptides from human serum samples with antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The dual functional TE microchips could provide high throughput for online protein digestion and glycopeptide enrichment, showing great promise for future extended applications in proteomics and the study of related diseases.
    MeSH term(s) Humans ; Glycopeptides/chemistry ; Immunoglobulin G ; Osteopontin ; Sulfhydryl Compounds ; Microfluidics ; COVID-19 ; SARS-CoV-2 ; Inflammation ; Digestion
    Chemical Substances Glycopeptides ; Immunoglobulin G ; Osteopontin (106441-73-0) ; Sulfhydryl Compounds
    Language English
    Publishing date 2023-01-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 201093-8
    ISSN 1618-2650 ; 0016-1152 ; 0372-7920
    ISSN (online) 1618-2650
    ISSN 0016-1152 ; 0372-7920
    DOI 10.1007/s00216-022-04498-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 4' 69/97: Show issues
    Archiv 3: Show issues
    Z 9.4: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article: [Transport mechanism of isorhapontigenin based on human intestinal Caco-2 cells].

    Yuan, Zi-Shuo / Zhang, Ting-Ting / Jin, Bo / Li, Tong / Ma, Chen

    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

    2017  Volume 42, Issue 3, Page(s) 587–592

    Abstract: Isorhapontigenin (ISO) is suggested to have many different kinds of pharmacology activities, such as anti-inflammatory effect, anti-oxidation effect and anti-cancer effect. This paper mainly discussed the transport mechanism of ISO in Caco-2 cell models. ...

    Abstract Isorhapontigenin (ISO) is suggested to have many different kinds of pharmacology activities, such as anti-inflammatory effect, anti-oxidation effect and anti-cancer effect. This paper mainly discussed the transport mechanism of ISO in Caco-2 cell models. The concentration of ISO was determined by UPLC method with PDA detector at 310 nm, and then the apparent permeability coefficient Papp was calculated. The cytotoxic of different concentrations of ISO was investigated on Caco-2 cells to determine the concentration of drug administration. The effects of ISO concentration, time, temperature and transporter inhibitors on the transport of ISO were investigated. The test results showed that, ISO didn't have significant cytotoxicity at 10-60 μmol•L ⁻¹ in 14 hours. The transportation of ISO on Caco-2 cells was related to the concentration to a certain extent. Papp of ISO was higher than 10×10-6 cm•s ⁻¹ and ISO was absorbed easily by Caco-2 cells. The transport volume of ISO at BL side reached maximum at 3 h and was slightly decreased at 6 h. Papp (AP-BL) and Papp(BL-AP) at 4 ℃ were lower than those at 37 ℃. Papp (AP-BL) of ISO was significantly increased after adding P-gp inhibitor verapamil and Papp (BL-AP) of ISO was significantly decreased after adding MRP-2 inhibitor (probenecid or MK-571). The results suggested that transport mode of ISO was mainly passive diffusion in Caco-2 cell models, and P-gp and MRP may be involved in the transport of ISO.
    Language Chinese
    Publishing date 2017-02
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 1004649-5
    ISSN 1001-5302 ; 0254-0029
    ISSN 1001-5302 ; 0254-0029
    DOI 10.19540/j.cnki.cjcmm.20170103.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3056: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Discovery of Fluorine-Containing Benzoxazinyl-oxazolidinones for the Treatment of Multidrug Resistant Tuberculosis.

    Zhao, Hongyi / Lu, Yu / Sheng, Li / Yuan, Zishuo / Wang, Bin / Wang, Weiping / Li, Yan / Ma, Chen / Wang, Xiaoliang / Zhang, Dongfeng / Huang, Haihong

    ACS medicinal chemistry letters

    2017  Volume 8, Issue 5, Page(s) 533–537

    Abstract: A novel series of fluorine-containing benzoxazinyl-oxazolidinones were designed and synthesized as antidrug-resistant tuberculosis agents possessing good activity and improved pharmacokinetic profiles. ... ...

    Abstract A novel series of fluorine-containing benzoxazinyl-oxazolidinones were designed and synthesized as antidrug-resistant tuberculosis agents possessing good activity and improved pharmacokinetic profiles. Compound
    Language English
    Publishing date 2017-04-12
    Publishing country United States
    Document type Journal Article
    ISSN 1948-5875
    ISSN 1948-5875
    DOI 10.1021/acsmedchemlett.7b00068
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top