Article: Revealing the Effects of Endoplasmic Reticulum Stress on Ferroptosis by Two-Channel Real-Time Imaging of pH and Viscosity
Analytical chemistry. 2022 Apr. 18, v. 94, no. 17
2022
Abstract: Endoplasmic reticulum (ER) is sensitive to changes in the intracellular environment such as pH and viscosity, and slight changes may trigger stress response. Besides, different from apoptosis and necrosis, ferroptosis is the result of lipid peroxidation ... ...
| Abstract | Endoplasmic reticulum (ER) is sensitive to changes in the intracellular environment such as pH and viscosity, and slight changes may trigger stress response. Besides, different from apoptosis and necrosis, ferroptosis is the result of lipid peroxidation accumulation. There is evidence that ferroptosis is closely related to endoplasmic reticulum stress (ERS). However, the possible changes in the pH and viscosity of the ER during the ferroptosis process have not yet been studied. Therefore, we used a new type of ER-targeted dual-excitation fluorescent probe (DSPI-3) to investigate the possible changes of pH and viscosity of ER during the ferroptosis. The novel probe DSPI-3 exhibited a highly sensitive and selective response to pH and viscosity. During the bioimaging process, it was found that the ER acidified and viscosity increased during the ferroptosis process induced by erastin, while the cells treated with ferrostatin-1 did not alter significantly. In addition, when dithiothreitol (DTT) and erastin stimulated the cells at the same time, we discovered that ER was acidified considerably at short notice, but the pH was slightly increased in the later stage. Besides, the change of the viscosity enhanced slowly with the passage of time, and there was a noteworthy decline in the later stage, demonstrating that the DTT-induced ERS accelerated the process of ferroptosis. We hope that this unique fluorescent probe can provide an effective method for studying the relationship between ERS and ferroptosis. |
|---|---|
| Keywords | analytical chemistry ; apoptosis ; bioimaging ; dithiothreitol ; endoplasmic reticulum ; endoplasmic reticulum stress ; ferroptosis ; fluorescent dyes ; lipid peroxidation ; necrosis ; pH ; stress response ; viscosity |
| Language | English |
| Dates of publication | 2022-0418 |
| Size | p. 6557-6565. |
| Publishing place | American Chemical Society |
| Document type | Article |
| ZDB-ID | 1508-8 |
| ISSN | 1520-6882 ; 0003-2700 |
| ISSN (online) | 1520-6882 |
| ISSN | 0003-2700 |
| DOI | 10.1021/acs.analchem.2c00387 |
| Database | NAL-Catalogue (AGRICOLA) |
More links
Kategorien
In stock of ZB MED Bonn / Germany
| Z 4' 52/94: Show issues | ||||
| Z 2.9: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.