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  1. Article ; Online: Identification of QTNs, QTN-by-environment interactions, and their candidate genes for salt tolerance related traits in soybean.

    Chen, Ying / Yue, Xiu-Li / Feng, Jian-Ying / Gong, Xin / Zhang, Wen-Jie / Zuo, Jian-Fang / Zhang, Yuan-Ming

    BMC plant biology

    2024  Volume 24, Issue 1, Page(s) 316

    Abstract: Background: Salt stress significantly reduces soybean yield. To improve salt tolerance in soybean, it is important to mine the genes associated with salt tolerance traits.: Results: Salt tolerance traits of 286 soybean accessions were measured four ... ...

    Abstract Background: Salt stress significantly reduces soybean yield. To improve salt tolerance in soybean, it is important to mine the genes associated with salt tolerance traits.
    Results: Salt tolerance traits of 286 soybean accessions were measured four times between 2009 and 2015. The results were associated with 740,754 single nucleotide polymorphisms (SNPs) to identify quantitative trait nucleotides (QTNs) and QTN-by-environment interactions (QEIs) using three-variance-component multi-locus random-SNP-effect mixed linear model (3VmrMLM). As a result, eight salt tolerance genes (GmCHX1, GsPRX9, Gm5PTase8, GmWRKY, GmCHX20a, GmNHX1, GmSK1, and GmLEA2-1) near 179 significant and 79 suggested QTNs and two salt tolerance genes (GmWRKY49 and GmSK1) near 45 significant and 14 suggested QEIs were associated with salt tolerance index traits in previous studies. Six candidate genes and three gene-by-environment interactions (GEIs) were predicted to be associated with these index traits. Analysis of four salt tolerance related traits under control and salt treatments revealed six genes associated with salt tolerance (GmHDA13, GmPHO1, GmERF5, GmNAC06, GmbZIP132, and GmHsp90s) around 166 QEIs were verified in previous studies. Five candidate GEIs were confirmed to be associated with salt stress by at least one haplotype analysis. The elite molecular modules of seven candidate genes with selection signs were extracted from wild soybean, and these genes could be applied to soybean molecular breeding. Two of these genes, Glyma06g04840 and Glyma07g18150, were confirmed by qRT-PCR and are expected to be key players in responding to salt stress.
    Conclusions: Around the QTNs and QEIs identified in this study, 16 known genes, 6 candidate genes, and 8 candidate GEIs were found to be associated with soybean salt tolerance, of which Glyma07g18150 was further confirmed by qRT-PCR.
    MeSH term(s) Glycine max/genetics ; Glycine max/physiology ; Salt Tolerance/genetics ; Quantitative Trait Loci/genetics ; Polymorphism, Single Nucleotide ; Gene-Environment Interaction ; Genes, Plant ; Phenotype
    Language English
    Publishing date 2024-04-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2059868-3
    ISSN 1471-2229 ; 1471-2229
    ISSN (online) 1471-2229
    ISSN 1471-2229
    DOI 10.1186/s12870-024-05021-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Autographa californica multiple nucleopolyhedrovirus orf132 encodes a nucleocapsid-associated protein required for budded-virus and multiply enveloped occlusion-derived virus production.

    Yang, Ming / Wang, Shuo / Yue, Xiu-Li / Li, Lu-Lin

    Journal of virology

    2014  Volume 88, Issue 21, Page(s) 12586–12598

    Abstract: Unlabelled: Autographa californica multiple nucleopolyhedrovirus orf132 (named ac132) has homologs in all genome-sequenced group I nucleopolyhedroviruses. Its role in the viral replication cycle is unknown. In this study, ac132 was shown to express a ... ...

    Abstract Unlabelled: Autographa californica multiple nucleopolyhedrovirus orf132 (named ac132) has homologs in all genome-sequenced group I nucleopolyhedroviruses. Its role in the viral replication cycle is unknown. In this study, ac132 was shown to express a protein of around 28 kDa, which was determined to be associated with the nucleocapsids of both occlusion-derived virus and budded virus. Confocal microscopy showed that AC132 protein appeared in central region of the nucleus as early as 12 h postinfection with the virus. It formed a ring zone at the periphery of the nucleus by 24 h postinfection. To investigate its role in virus replication, ac132 was deleted from the viral genome by using a bacmid system. In the Sf9 cell culture transfected by the ac132 knockout bacmid, infection was restricted to single cells, and the titer of infectious budded virus was reduced to an undetectable level. However, viral DNA replication and the expression of late genes vp39 and odv-e25 and a reporter gene under the control of the very late gene p10 promoter were unaffected. Electron microscopy showed that nucleocapsids, virions, and occlusion bodies were synthesized in the cells transfected by an ac132 knockout bacmid, but the formation of the virogenic stroma and occlusion bodies was delayed, the numbers of enveloped nucleocapsids were reduced, and the occlusion bodies contained mainly singly enveloped nucleocapsids. AC132 was found to interact with envelope protein ODV-E18 and the viral DNA-binding protein P6.9. The data from this study suggest that ac132 possibly plays an important role in the assembly and envelopment of nucleocapsids.
    Importance: To our knowledge, this is the first report on a functional analysis of ac132. The data presented here demonstrate that ac132 is required for production of the budded virus and multiply enveloped occlusion-derived virus of Autographa californica multiple nucleopolyhedrovirus. This article reveals unique phenotypic changes induced by ac132 deletion on the virus and multiple new findings on ac132.
    MeSH term(s) Animals ; Gene Deletion ; Microscopy, Electron, Transmission ; Molecular Weight ; Nucleocapsid/genetics ; Nucleocapsid/metabolism ; Nucleocapsid/ultrastructure ; Nucleopolyhedroviruses/genetics ; Nucleopolyhedroviruses/physiology ; Sf9 Cells ; Spodoptera ; Viral Structural Proteins/chemistry ; Viral Structural Proteins/genetics ; Viral Structural Proteins/metabolism ; Virion/ultrastructure ; Virus Assembly ; Virus Release
    Chemical Substances Viral Structural Proteins
    Language English
    Publishing date 2014-08-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.01313-14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 609: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: [Effects of mutations in the autographa californica multiple nucleopolyhedrovirus E25 on its trafficking to nucleus and budded virus production].

    Luo, Xiao-chun / Yue, Xiu-li / Li, Lu-lin

    Bing du xue bao = Chinese journal of virology

    2013  Volume 29, Issue 5, Page(s) 535–543

    Abstract: This study was performed to investigate the effects of different regions of the Autographa califor nica multiple nucleopolyhedrovirus envelope protein E25 on its trafficking into nucleus and nuclear localization in host cells and on virus replication. ... ...

    Abstract This study was performed to investigate the effects of different regions of the Autographa califor nica multiple nucleopolyhedrovirus envelope protein E25 on its trafficking into nucleus and nuclear localization in host cells and on virus replication. Fourteen recombinant bacmids, each containing an e25 mutant with substitution or insertion of egfp, in the absence or presence of the native e25, were constructed and used to transfect Sf9 cells. The E25-EGFP fusion proteins and native E25 expressed in the cells transfect ed with individual recombinant bacmid were traced by autofluorescence from EGFP or by immuno-fluorescence assays. Confocal microscopy revealed that the E25-EGFP fusion protein with the N-domain (2-45aa) of E25 substituted by EGFP only distributed in the cytoplasm in transfected cells; and the fusion protein with EGFP inserted at the laa/2aa site of E25 completely remained outside of the nucleus and resided along the nuclear membrane. The E25-EGFPs with 46-118aa of E25 substituted by EGFP or with EGFP inserted at the 118aa/119aa site were present outside, across from the nuclear membrane or in nuclear plasm in dot-like shapes. The fusion proteins with the C-domain substituted by EGFP or with EGFP inserted at the site of 45/46aa or at the C-terminal formed a condensed ring or spread throughout the nucleus, in a similar manner to the E25 distributed in the cells transfected by the e25-knockout repair bacmid. These results prove that the N-terminal domain is critical for nuclear transportation of E25 and possibly to its position on the cytoplasm membrane as well; and the sequence downstream of the N-terminal domain also affects trafficking and nuclear localization of the protein. In cells transfected with bacmids containing both the native e25 and individual e25-egfp mutants, the E25-EGFP fusion proteins co-localized with E25 individually, showing similar patterns of subcellular localization as E25 mutants in the absence of native E25 in most cases, suggesting that the E25 likely exists and functions as dimmers or polymers. Production of infectious BV was dramatically reduced and even completely eliminated in most cases, either in the absence or presence of the native e25.
    MeSH term(s) Amino Acid Motifs ; Animals ; Cell Nucleus/metabolism ; Cell Nucleus/virology ; Mutation ; Nucleopolyhedrovirus/chemistry ; Nucleopolyhedrovirus/genetics ; Nucleopolyhedrovirus/physiology ; Protein Transport ; Spodoptera/virology ; Viral Proteins/chemistry ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Virus Release ; Virus Replication
    Chemical Substances Viral Proteins
    Language Chinese
    Publishing date 2013-09
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1158410-5
    ISSN 1000-8721
    ISSN 1000-8721
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4131: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Chitosan stabilized Prussian blue nanoparticles for photothermally enhanced gene delivery.

    Li, Xiao-Da / Liang, Xiao-Long / Ma, Fang / Jing, Li-Jia / Lin, Li / Yang, Yong-Bo / Feng, Shan-Shan / Fu, Guang-Lei / Yue, Xiu-Li / Dai, Zhi-Fei

    Colloids and surfaces. B, Biointerfaces

    2014  Volume 123, Page(s) 629–638

    Abstract: The lack of biosafety and insufficient delivery efficiency of gene-carriers are still obstacles to human gene therapy. This paper reported highly biocompatible chitosan (CS) functionalized Prussian blue (PB) nanoparticles (designated as CS/PB NPs) for ... ...

    Abstract The lack of biosafety and insufficient delivery efficiency of gene-carriers are still obstacles to human gene therapy. This paper reported highly biocompatible chitosan (CS) functionalized Prussian blue (PB) nanoparticles (designated as CS/PB NPs) for photocontrollable gene delivery. The ultra-small size (∼3 nm), positive charge and high physiological stability of CS/PB NPs make it suitable to be a nonviral vector. In addition, CS/PB NPs could effectively convert the near infrared (NIR) light into heat due to its strong absorption in the NIR region, assisting the uptake of NPs by cells. Upon NIR light irradiation, CS/PB NPs showed superior gene transfection efficiency, much higher than that of free polyethylenimine (PEI). Both in vitro and in vivo experiments demonstrated that CS/PB NPs had excellent biocompatiblity. This work also encourages further exploration of the CS/PB NPs as a photocontrollable nanovector for combined photothermal and gene therapy.
    MeSH term(s) Chitosan/chemistry ; Genetic Vectors ; HeLa Cells ; Humans ; Nanoparticles/chemistry
    Chemical Substances Chitosan (9012-76-4)
    Language English
    Publishing date 2014-11-01
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1500523-9
    ISSN 1873-4367 ; 0927-7765
    ISSN (online) 1873-4367
    ISSN 0927-7765
    DOI 10.1016/j.colsurfb.2014.10.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Imaging guided photothermal therapy using iron oxide loaded poly(lactic acid) microcapsules coated with graphene oxide.

    Li, Xiao-Da / Liang, Xiao-Long / Yue, Xiu-Li / Wang, Jin-Rui / Li, Chang-Hui / Deng, Zi-Jian / Jing, Li-Jia / Lin, Li / Qu, En-Ze / Wang, Shu-Min / Wu, Chun-Long / Wu, Hua-Xing / Dai, Zhi-Fei

    Journal of materials chemistry. B

    2013  Volume 2, Issue 2, Page(s) 217–223

    Abstract: A novel multifunctional theranostic agent has been successfully fabricated by loading iron oxide nanoparticles into poly(lactic acid) (PLA) microcapsules followed by surface functionalization with graphene oxide. Both in vitro and in vivo experiments ... ...

    Abstract A novel multifunctional theranostic agent has been successfully fabricated by loading iron oxide nanoparticles into poly(lactic acid) (PLA) microcapsules followed by surface functionalization with graphene oxide. Both in vitro and in vivo experiments proved that the resulting microcapsules could serve as contrast agents to simultaneously enhance ultrasound, magnetic resonance and photoacoustic imaging. The composite microcapsules show good biocompatibility and rapid response to magnetic fields. Due to the strong absorption of the near-infrared light, the composite microcapsules could efficiently kill cancer cells upon NIR laser irradiation. In addition, it was found that such a photothermal effect could be obviously enhanced by applying an external magnetic field. In a nutshell, this multifunctional microcapsule can be developed as a promising platform that integrates multimodality imaging and therapy capabilities for effective cancer theranostics.
    Language English
    Publishing date 2013-11-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 2702241-9
    ISSN 2050-7518 ; 2050-750X
    ISSN (online) 2050-7518
    ISSN 2050-750X
    DOI 10.1039/c3tb21281e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Imaging guided photothermal therapy using iron oxide loaded poly(lactic acid) microcapsules coated with graphene oxide

    Li, Xiao-Da / Dai, Zhi-Fei / Deng, Zi-Jian / Jing, Li-Jia / Li, Chang-Hui / Liang, Xiao-Long / Lin, Li / Qu, En-Ze / Wang, Jin-Rui / Wang, Shu-Min / Wu, Chun-Long / Wu, Hua-Xing / Yue, Xiu-Li

    Journal of materials chemistry B. 2013 Dec. 4, v. 2, no. 2

    2013  

    Abstract: A novel multifunctional theranostic agent has been successfully fabricated by loading iron oxide nanoparticles into poly(lactic acid) (PLA) microcapsules followed by surface functionalization with graphene oxide. Both in vitro and in vivo experiments ... ...

    Abstract A novel multifunctional theranostic agent has been successfully fabricated by loading iron oxide nanoparticles into poly(lactic acid) (PLA) microcapsules followed by surface functionalization with graphene oxide. Both in vitro and in vivo experiments proved that the resulting microcapsules could serve as contrast agents to simultaneously enhance ultrasound, magnetic resonance and photoacoustic imaging. The composite microcapsules show good biocompatibility and rapid response to magnetic fields. Due to the strong absorption of the near-infrared light, the composite microcapsules could efficiently kill cancer cells upon NIR laser irradiation. In addition, it was found that such a photothermal effect could be obviously enhanced by applying an external magnetic field. In a nutshell, this multifunctional microcapsule can be developed as a promising platform that integrates multimodality imaging and therapy capabilities for effective cancer theranostics.
    Keywords absorption ; biocompatibility ; graphene oxide ; image analysis ; in vivo studies ; iron oxides ; irradiation ; magnetic fields ; nanoparticles ; near infrared radiation ; neoplasm cells ; neoplasms ; photothermotherapy ; polylactic acid ; precision medicine ; ultrasonics
    Language English
    Dates of publication 2013-1204
    Size p. 217-223.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 2702241-9
    ISSN 2050-7518 ; 2050-750X
    ISSN (online) 2050-7518
    ISSN 2050-750X
    DOI 10.1039/c3tb21281e
    Database NAL-Catalogue (AGRICOLA)

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