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Article ; Online: Purification and Characterization Laccase from

Yuliana, T / Putri, R Ashifa / Hanidah, I / Mardawati, E / Tjaturina, H

Pakistan journal of biological sciences : PJBS

2023 Volume 25, Issue 12, Page(s) 1077–1084

Abstract: ... Background and Objective: ... Laccase is classified as an oxidoreductase enzyme that catalyzes oxidation reactions of phenolic groups by using oxygen as its electron acceptor. Laccase isolated from ... Trametes versicolor ... (L.) Lloyd has a wide ... ...

Abstract	Background and Objective: Laccase is classified as an oxidoreductase enzyme that catalyzes oxidation reactions of phenolic groups by using oxygen as its electron acceptor. Laccase isolated from Trametes versicolor (L.) Lloyd has a wide range of applications in the industrial sector. The use of enzymes in the industrial sector requires pure enzyme conditions from impurities so that the enzyme can maximize its ability in converting the substrate. This study aims to obtain enzyme activity and the characteristic of purified laccase enzymes isolated from Trametes versicolor (L.) Lloyd. Materials and Methods: This study was conducted with an experimental method followed by descriptive analysis. The steps of this research consist of a qualitative assay of laccase enzyme, crude laccase extract desalting by Sephadex G-25, laccase purification by Sephadex G-100 and laccase optimum pH characterization. Results: The result of this study showed that purification of laccase from Trametes versicolor (L.) Lloyd with Sephadex G-25 increases laccase enzyme-specific activity which is 10.966 U mg<sup>1</sup> and reaches 2.93-fold purity. The highest laccase enzyme activity was achieved at pH 4 with a value of laccase activity 62.39 U L<sup>1</sup>. Conclusion: Based on current results, purifying laccase from Trametes versicolor (L.) Lloyd with Sephadex G-25 was recommended which resulting higher enzyme specific activity.
MeSH term(s)	Fermentation ; Trametes/chemistry ; Trametes/metabolism ; Laccase/metabolism ; Polyporaceae
Chemical Substances	Laccase (EC 1.10.3.2)
Language	English
Publishing date	2023-03-29
Publishing country	Pakistan
Document type	Journal Article
ISSN	1812-5735
ISSN (online)	1812-5735
DOI	10.3923/pjbs.2022.1077.1084
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Partial Purification of Bacteriocin from

Yuliana, T / Hayati, F / Rialita, T / Cahyana, Y

Pakistan journal of biological sciences : PJBS

2021 Volume 25, Issue 9, Page(s) 796–802

Abstract: ... Background and Objective: ... Preservation using antimicrobials has been observed to inhibit the growth of pathogenic bacteria in food. Nowadays many people choose food preservatives that are safe for health and natural. Bacteriocins as food ... ...

Abstract	Background and Objective: Preservation using antimicrobials has been observed to inhibit the growth of pathogenic bacteria in food. Nowadays many people choose food preservatives that are safe for health and natural. Bacteriocins as food preservatives are safe because antimicrobials from the antimicrobial peptide group include GRAS (Generally Recognized As Safe). Bacteriocin-producing LAB can be found in various fermented foods, one of which is "Dadih". Bacteriocins are expected to inhibit the growth of pathogenic bacteria so that they can be developed as an alternative to food preservatives. Materials and Methods: In this study, all experiments were performed with two replicates and the results were expressed as Mean±Standard Deviation (SD). Results: Screening results showed that the DK8 isolate had the highest antimicrobial activity. The DK8 isolate was identified molecularly using 16s RNA sequencing, showing that the DK8 isolate had the highest similarity to Lactobacillus pentosus strain 124-2. Bacteriocins from DK8 isolate and partially purified using ammonium sulfate precipitation at concentrations of 50, 60 and 70%. The addition of ammonium sulfate with a concentration of 50% showed the highest antimicrobial activity against Salmonella sp. (12.63 mm) and Escherichia coli (11.33 mm) while the highest antimicrobial activity against Staphylococcus aureus was the addition of 60% ammonium sulfate (8.13 mm). Conclusion: Lactic acid bacteria isolate was identified to have the highest similarity with Lactobacillus pentosus strain 124-2 and precipitation using 50% ammonium sulfate showed the highest antimicrobial activity.
MeSH term(s)	Ammonium Sulfate ; Anti-Infective Agents/pharmacology ; Bacteriocins/pharmacology ; Escherichia coli ; Food Preservatives/pharmacology ; Humans ; Lactobacillus pentosus
Chemical Substances	Anti-Infective Agents ; Bacteriocins ; Food Preservatives ; Ammonium Sulfate (SU46BAM238)
Language	English
Publishing date	2021-11-03
Publishing country	Pakistan
Document type	Journal Article
ISSN	1812-5735
ISSN (online)	1812-5735
DOI	10.3923/pjbs.2022.796.802
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Article ; Online: Study on the Development and Functional Characteristics of Salted Egg with Liquid Smoke.

Harlina, Putri Widyanti / Yuliana, Tri / Fetriyuna / Shahzad, Raheel / Ma, Meihu

Food science of animal resources

2023 Volume 43, Issue 3, Page(s) 471–490

Abstract: In this study, the duck eggs were salted with none or 2.5% and 5.0% (v/v) of liquid smoke (LS), respectively. As a control, samples salted without LS were used. The 2-thiobarbituric acid (TBA) values, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ...

Abstract	In this study, the duck eggs were salted with none or 2.5% and 5.0% (v/v) of liquid smoke (LS), respectively. As a control, samples salted without LS were used. The 2-thiobarbituric acid (TBA) values, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, and reducing power of the three groups were tested at 0, 7, 14, and 21 and 28 days to determine the effects of LS on the antioxidant activity of treated eggs. In addition, gas chromatography-mass spectrometry (GC-MS) and electronic nose (E-Nose) were used to analyze the volatile flavor components of fresh duck eggs, LS, control, and salted duck eggs enriched with 2.5% (v/v) LS after 28 days of salting. The TBA value considerably increased with an increase in salting period, and the treated egg's TBA value significantly associated with LS concentration. The TBA value decreased as the LS concentration increased. The amount of LS present was highly associated with their capacity to scavenge DPPH radicals. The reducing power of the samples was substantially correlated with the LS concentration, and the reducing power increased with increasing LS concentration. The GC-MS data revealed that phenols and ketones were the predominant chemicals present in the LS, and they were also found in the eggs added to the LS even though they were absent in the fresh eggs and control. The flavor of the control group and treated eggs with LS differed significantly, according to the principal component analysis and radar map of the E-nose. The texture study results revealed that the LS significantly impacted the hardness, cohesiveness, and chewiness of eggs.
Language	English
Publishing date	2023-05-01
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	3016289-0
ISSN	2636-0780 ; 2636-0772
ISSN (online)	2636-0780
ISSN	2636-0772
DOI	10.5851/kosfa.2023.e10
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Article ; Online: Potential of Lignocellulosic Waste for Laccase Production by

Yuliana, T / Komara, D Z / Saripudin, G L U / Subroto, E / Safitri, R

Pakistan journal of biological sciences : PJBS

2021 Volume 24, Issue 6, Page(s) 699–705

Abstract: ... Background and Objective: ... Laccase is one of the ligninolytic enzymes classified as a multicopper oxidoreductase group that has the ability in oxidizing phenolic compounds and has widespread use in both food and non-food industries. This enzyme is ... ...

Abstract	Background and Objective: Laccase is one of the ligninolytic enzymes classified as a multicopper oxidoreductase group that has the ability in oxidizing phenolic compounds and has widespread use in both food and non-food industries. This enzyme is extracellularly secreted by white-rot fungi, Trametes versicolor (L.) Lloyd in the media containing lignocellulose, for example, kapok banana peels and sawdust. The objective of this study was to evaluate lignocellulosic substrate that able to produce the highest activity of the laccase from the T. versicolor (L.) Lloyd. Materials and Methods: Three substrate variations used in this work included the cultivation media with the addition of either kapok banana peels or sawdust and without using both materials. The inducer (CuSO<sub>4</sub>) was added to each substrate variation and the laccase activity was subsequently measured. Results: The qualitative test result for laccase detection showed that T. versicolor (L.) Lloyd was able to produce this enzyme indicated with a reddish-brown surrounding fungal colony. The fungi cultivated in media with the content of sawdust and 1 mM CuSO<sub>4</sub> yielded the highest laccase activity, reaching 573.6 U L<sup></sup><sup>1</sup> with an OD value of 0.5567 and a pH of 5.3 after 7 days of incubation. Meanwhile, the addition of kepok banana peels and 1 mM CuSO<sub>4</sub>, showed the maximum laccase activity (297.7 U L<sup></sup><sup>1</sup>) with the OD value of 0.6932 and a pH of 5 after incubation for 6 days. Conclusion: The white-rot fungi of T. versicolor (L.) Lloyd could produce optimal laccase activity by adding sawdust substrate and 1 mM CuSO<sub>4 </sub>inducer on submerged fermentation.
MeSH term(s)	Fermentation ; Fruit/microbiology ; Fungal Proteins/metabolism ; Hydrogen-Ion Concentration ; Immersion ; Industrial Microbiology ; Laccase/metabolism ; Lignin/metabolism ; Musa/microbiology ; Polyporaceae/enzymology ; Substrate Specificity ; Time Factors ; Waste Products ; Wood/microbiology
Chemical Substances	Fungal Proteins ; Waste Products ; lignocellulose (11132-73-3) ; Lignin (9005-53-2) ; Laccase (EC 1.10.3.2)
Language	English
Publishing date	2021-09-13
Publishing country	Pakistan
Document type	Journal Article
ISSN	1812-5735
ISSN (online)	1812-5735
DOI	10.3923/pjbs.2021.699.705
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Article ; Online: Study of <i>Ganoderma lucidum </i>in Laccase Production using Corncob and Paddies Straw Substrates on Submerged Fermentation System.

Yuliana, T / Putri, N Z / Komara, D Z / Mardawati, E / Lanti, I / Rahimah, S

Pakistan journal of biological sciences : PJBS

2020 Volume 23, Issue 8, Page(s) 1060–1065

Abstract: Background and objective: Ganoderma lucidum a white rot fungi, produce laccase which capable to degrade lignin due to its activity as ligninolytic enzymes. The production of laccase by G. lucidum using various agroindustrial wastes, including corncob ... ...

Abstract	Background and objective: Ganoderma lucidum a white rot fungi, produce laccase which capable to degrade lignin due to its activity as ligninolytic enzymes. The production of laccase by G. lucidum using various agroindustrial wastes, including corncob and paddies straw, as substrates have been studied. The purpose of this study was to determine substrate that able to produce the highest activity of the laccase from the G. lucidum. Materials and methods: The method used an experimental design followed by descriptive analysis using 4 treatments with duplication including treatment G. lucidum growth into A (control, Potato Dextrose Broth (PDB)), B (PDB+corncob), C (PDB+rice straw) and D (PDB+corncob+rice straw). This study includes; (1) Qualitative assay determination of laccase, (2) Extraction and laccase activity, (3) Cell concentration of G. lucidum measurement and (4) pH measurement. Results: Laccase qualitative assay showed brownish red ring on PDA media that indicated a positive of the laccase enzyme secreted by G. lucidum. Enzyme activity under submerged fermentation condition was achieved by the treatment of adding corncobs with the highest activity accounting 68.75 U mL-1. The fermentation process causes a decrease in pH during the incubation time to pH 4.83. The results of pH measurements showed that the laccase enzyme from G. lucidum worked optimally at pH 4-5 achieved after 5 day of incubation. Conclusion: Our results suggest that G. lucidum has potential to produce laccase enzyme by using substrate comprising corncob and rice straw on submerged fermentation.
MeSH term(s)	Basidiomycota/enzymology ; Culture Media/metabolism ; Fermentation ; Fungal Proteins/metabolism ; Laccase/biosynthesis ; Lignin/metabolism ; Oryza/metabolism ; Zea mays/metabolism
Chemical Substances	Culture Media ; Fungal Proteins ; Lignin (9005-53-2) ; Laccase (EC 1.10.3.2)
Language	English
Publishing date	2020-07-23
Publishing country	Pakistan
Document type	Journal Article
ISSN	1812-5735
ISSN (online)	1812-5735
DOI	10.3923/pjbs.2020.1060.1065
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Article ; Online: The Effect of Dual-Modification by Heat-Moisture Treatment and Octenylsuccinylation on Physicochemical and Pasting Properties of Arrowroot Starch.

Marta, Herlina / Rismawati, Ari / Soeherman, Giffary Pramafisi / Cahyana, Yana / Djali, Mohamad / Yuliana, Tri / Sondari, Dewi

Polymers

2023 Volume 15, Issue 15

Abstract: Starch is widely applied in various industrial sectors, including the food industry. Starch is used as a thickener, stabilizer, or emulsifier. However, arrowroot starch generally has weaknesses, such as unstable under heating and acidic conditions, which ...

Abstract	Starch is widely applied in various industrial sectors, including the food industry. Starch is used as a thickener, stabilizer, or emulsifier. However, arrowroot starch generally has weaknesses, such as unstable under heating and acidic conditions, which are generally applied to processing in the food industry. Modifications were applied to improve the characteristics of native arrowroot starch. In this study, arrowroot starch was modified by heat-moisture treatment (HMT), octenylsuccinylation (OSA), and dual modification between OSA and HMT in a different sequence--namely, HMT followed by OSA, and OSA followed by HMT. This study aims to determine the effect of different modification methods on the physicochemical and functional properties of native arrowroot starch. The result shows that both single HMT and dual modification caused damage to native starch granules, such as the formation of cracks and roughness. For single OSA treatment, especially, there is no significant change in granule morphology after modification. All modification treatments did not change the crystalline type of starch but reduced the RC of native starch. Both single HMT and dual modifications (HMT-OSA, OSA-HMT) increased pasting temperature and setback, but, conversely, decreased the peak and the breakdown viscosity of native starch, whereas single OSA had the opposite trend compared with the other modifications. HMT played a greater role in increasing the thermal stability and the retrogradation ability of arrowroot starch. Both single modifications (HMT and OSA) increased the hardness and gumminess of native starch, and the opposite was true for the dual modifications. HMT had a greater effect on color characteristics, where the lightness and whiteness index of native arrowroot starch decreased. Single OSA modification increased swelling volume higher than dual modification. Both single HMT and dual modifications increased water absorption capacity and decreased the oil absorption capacity of native arrowroot starch.
Language	English
Publishing date	2023-07-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym15153215
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Article: Mass Effect of Coconut Shell-derived Activated Carbon on Adsorption of Benzene, Toluene, Ethylbenzene, and Xylene in Motorcycle Emissions

Yuliana, Trisna / Rohmah, Aini Aspiati / Maulana, Yusuf Eka / Hardian, Arie

Jurnal Kimia Sains dan Aplikasi. 2021 Apr., v. 24, no. 4

2021

Abstract: The use of motorized transportation in Indonesia is now proliferating. The higher the use of motorized vehicle-based transportation in an area, the higher the potential for air pollution. One of the air pollutants is a mixture of benzene, toluene, ... ...

Abstract	The use of motorized transportation in Indonesia is now proliferating. The higher the use of motorized vehicle-based transportation in an area, the higher the potential for air pollution. One of the air pollutants is a mixture of benzene, toluene, ethylbenzene, and xylene (BTEX). This study examines the effect of coconut shell-derived activated carbon adsorbent mass which is adjusted with different thicknesses on its adsorption ability for BTEX. The adsorbent is used to adsorb the emissions of the 1990 GL-Pro motorcycle with premium fuel. The results of gas chromatography-mass spectroscopy (GC-MS) show that motor vehicle emissions contain BTEX and other hydrocarbons. ANOVA variant analysis showed that the difference in mass of activated carbon in the range of this study did not provide a significant difference in BTEX adsorption. Keywords: adsorbent; motor vehicle emissions; BTEX pollutants; coconut shell
Keywords	activated carbon ; adsorbents ; adsorption ; air ; air pollution ; benzene ; coconuts ; ethylbenzene ; motorcycles ; toluene ; transportation ; xylene ; Indonesia
Language	English
Dates of publication	2021-04
Size	p. 120-125.
Publishing place	Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
Document type	Article
ISSN	2597-9914
DOI	10.14710/jksa.24.4.120-125
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Indigenous Bacteriocin of Lactic Acid Bacteria from "Dadih" a Fermented Buffalo Milk from West Sumatra, Indonesia as Chicken Meat Preservative.

Yuliana, T / Hayati, F / Cahyana, Y / Rialita, T / Mardawati, E / Harahap, B M / Safitri, R

Pakistan journal of biological sciences : PJBS

2020 Volume 23, Issue 12, Page(s) 1572–1580

Abstract: Background and objective: The bacteriocin isolated from fermented buffalo milk from West Sumatra-Indonesia, called Dadih, can be considered as a natural and safe antimicrobial compound for food products. The objective of this research was to evaluate ... ...

Abstract	Background and objective: The bacteriocin isolated from fermented buffalo milk from West Sumatra-Indonesia, called Dadih, can be considered as a natural and safe antimicrobial compound for food products. The objective of this research was to evaluate the antimicrobial activity of bacteriocin from Dadih and its effectiveness as a preservative in chicken meat. Materials and methods: This study used experimental method followed by statistical analysis using 3 experiments with duplication including experiment of meat samples (0 and 10% bacteriocin), storage temperatures (7 and 26°C) and storage duration (0, 1, 2, 3, 4, 5, 6 days and 0, 6, 12 hrs). Each experiment consists of a bacteriocin test, antimicrobial activity assay, physicochemical measurement and storability. Results: From 10 LAB isolates successfully obtained from Dadih, two isolates with D7 code and D10 code had the highest antimicrobial activity, reaching 11.75 mm and 12 mm, respectively. The meat treated by 10% of bacteriocin gave the lower total microbial (3rd and 5th day) and total E. coli (5th day) at 7 and 26°C. The pH and water activity (aw) values of chicken meat with 10% of bacteriocin showed lower values at 7 and 26°C. The application of bacteriocin to chicken meat was able to inhibit the microbial growth that was still below standard for 3 days at 7°C and 6 hrs at 26°C. Conclusion: Based on research, lactic acid bacteria isolated from buffalo milk curd produced bacteriocin compound which has antimicrobial properties. This bacteriocin showed potential as a natural preservative for chicken meat by inhibiting the growth of pathogen microorganisms.
MeSH term(s)	Animals ; Bacteriocins/isolation & purification ; Bacteriocins/pharmacology ; Buffaloes ; Cultured Milk Products/microbiology ; Escherichia coli/drug effects ; Escherichia coli/growth & development ; Food Microbiology ; Food Preservation ; Food Preservatives/isolation & purification ; Food Preservatives/pharmacology ; Indonesia ; Lactobacillales/metabolism ; Poultry Products/microbiology ; Salmonella/drug effects ; Salmonella/growth & development ; Time Factors
Chemical Substances	Bacteriocins ; Food Preservatives
Language	English
Publishing date	2020-12-04
Publishing country	Pakistan
Document type	Journal Article
ISSN	1812-5735
ISSN (online)	1812-5735
DOI	10.3923/pjbs.2020.1572.1580
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Article: An Integrated Process for the Xylitol and Ethanol Production from Oil Palm Empty Fruit Bunch (OPEFB) Using

Mardawati, Efri / Febrianti, Emilda Ayu / Fitriana, Hana Nur / Yuliana, Tri / Putriana, Norisca Aliza / Suhartini, Sri / Kasbawati

Microorganisms

2022 Volume 10, Issue 10

Abstract: Oil palm empty fruit bunch (OPEFB) is the largest biomass waste from the palm oil industry. The OPEFB has a lignocellulose content of 34.77% cellulose, 22.55% hemicellulose, and 10.58% lignin. Therefore, this material's hemicellulose and cellulose ... ...

Abstract	Oil palm empty fruit bunch (OPEFB) is the largest biomass waste from the palm oil industry. The OPEFB has a lignocellulose content of 34.77% cellulose, 22.55% hemicellulose, and 10.58% lignin. Therefore, this material's hemicellulose and cellulose content have a high potential for xylitol and ethanol production, respectively. This study investigated the integrated microaerobic xylitol production by
Language	English
Publishing date	2022-10-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms10102036
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Article ; Online: Banana starch modified by heat moisture treatment and annealing: Study on digestion kinetics and enzyme affinity.

Cahyana, Yana / Annisa, Nadia Dewi Nur / Khoerunnisa, Tiara Kurnia / Sulastri, Sri / Marta, Herlina / Rialita, Tita / Yuliana, Tri / Aït-Kaddour, Abderrahmane / Şumnu, Gülüm

International journal of biological macromolecules

2023 Volume 258, Issue Pt 1, Page(s) 128771

Abstract: Starch modification by annealing (ANN) and heat-moisture treatment (HMT) results in a lower crystallinity compared to native but the change of B crystalline type to A type is only observed in HMT starch. All starches possess two different digestion rate ... ...

Abstract	Starch modification by annealing (ANN) and heat-moisture treatment (HMT) results in a lower crystallinity compared to native but the change of B crystalline type to A type is only observed in HMT starch. All starches possess two different digestion rate constants i.e. k
MeSH term(s)	Starch/chemistry ; Hot Temperature ; Musa/metabolism ; Chemical Phenomena ; Digestion
Chemical Substances	Starch (9005-25-8)
Language	English
Publishing date	2023-12-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.128771
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