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  1. Article ; Online: Design and structural characterization of autoinhibition-compromised full-length Ran

    Yuping Tan / Yuqing Zhang / Qiao Zhou / Da Jia / Qingxiang Sun

    Signal Transduction and Targeted Therapy, Vol 6, Iss 1, Pp 1-

    2021  Volume 3

    Keywords Medicine ; R ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: GEF-independent Ran activation shifts a fraction of the protein to the cytoplasm and promotes cell proliferation

    Jinhan Zhou / Yuping Tan / Yuqing Zhang / Aiping Tong / Xiaofei Shen / Xiaodong Sun / Da Jia / Qingxiang Sun

    Molecular Biomedicine, Vol 1, Iss 1, Pp 1-

    2020  Volume 13

    Abstract: Abstract Ran (Ras-related nuclear protein) plays several important roles in nucleo-cytoplasmic transport, mitotic spindle formation, nuclear envelope/nuclear pore complex assembly, and other functions in the cytoplasm, as well as in cellular ... ...

    Abstract Abstract Ran (Ras-related nuclear protein) plays several important roles in nucleo-cytoplasmic transport, mitotic spindle formation, nuclear envelope/nuclear pore complex assembly, and other functions in the cytoplasm, as well as in cellular transformation when switched on. Unlike other members of the GTPase superfamily, Ran binds more tightly to GDP than to GTP due to the presence of an auto-inhibitory C-terminal tail. Multiple missense mutations in the C-terminus of Ran occur in cancers, but their biological significance remains unclear. Here, the quantitative GDP/GTP binding preference of four engineered mutations with unstable C-termini was analyzed using a devised mant-GDP dissociation assay. The results showed that the impact of different C-terminal mutations depends on multiple factors. Although these mutants were more GTP-loaded in human cells, they were shown to be more cytoplasmic, and to support nuclear transport with minimally or partially reduced efficiency. Further, several Ran cancer mutants were compromised in autoinhibition, slightly more GTP-bound, more cytoplasmic, and enhanced the proliferation of A549 and HeLa cells in vitro. Thus, our work reveals a new route of Ran activation independent of guanine nucleotide exchange factor (GEF), which may account for the hyper-proliferation induced by Ran cancer mutations.
    Keywords Small GTPases ; Nuclear transport ; GTP bias ; Activation ; Cancer mutations ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Springer
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Efficacy and Safety of Clearing Heat and Detoxifying Injection in the Treatment of Influenza

    Yu Liu / Yuhong Huang / Baolin Wei / Xinqiao Liu / Yaqing Zhang / Xiaomin Huang / Yuping Tan / Zengtao Sun

    Evidence-Based Complementary and Alternative Medicine, Vol

    A Randomized, Double-Blinded, Placebo-Controlled Trial

    2014  Volume 2014

    Keywords Medicine ; R ; Medicine (General) ; R5-920
    Publishing date 2014-01-01T00:00:00Z
    Publisher Hindawi Publishing Corporation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Efficacy and Safety of Clearing Heat and Detoxifying Injection in the Treatment of Influenza

    Yu Liu / Yuhong Huang / Baolin Wei / Xinqiao Liu / Yaqing Zhang / Xiaomin Huang / Yuping Tan / Zengtao Sun

    Evidence-Based Complementary and Alternative Medicine, Vol

    A Randomized, Double-Blinded, Placebo-Controlled Trial

    2014  Volume 2014

    Keywords Medicine (General) ; R5-920 ; Medicine ; R
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Hindawi Publishing Corporation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Efficacy and Safety of Clearing Heat and Detoxifying Injection in the Treatment of Influenza

    Yu Liu / Yuhong Huang / Baolin Wei / Xinqiao Liu / Yaqing Zhang / Xiaomin Huang / Yuping Tan / Zengtao Sun

    Evidence-Based Complementary and Alternative Medicine, Vol

    A Randomized, Double-Blinded, Placebo-Controlled Trial

    2014  Volume 2014

    Abstract: Objective. To evaluate the efficacy and safety of CHDI in the treatment of influenza infection. Method. A randomized double-blind, double dummy trial was conducted. Influenza patients with a positive influenza A rapid test diagnosis were randomized to ... ...

    Abstract Objective. To evaluate the efficacy and safety of CHDI in the treatment of influenza infection. Method. A randomized double-blind, double dummy trial was conducted. Influenza patients with a positive influenza A rapid test diagnosis were randomized to receive CHDI or oseltamivir. Primary outcome was assessed by the median fever alleviation time and clearance time, and secondary outcome was total scores of influenza symptoms. Results. One hundred thirty-nine participants were screened and 34 had a RT-PCR laboratory confirmation of influenza virus infection. Fever alleviation time was 2.5 and 5 hours in CHDI and oseltamivir, respectively, and fever clearance time was 32.5 and 49 hours. The HR of fever alleviation and clearance time shows no significant difference between two groups. Total scores of influenza symptoms descended significantly in both groups after treatment and descended more in CHDI than oseltamivir on day 2. Similar to total symptoms severity score, fever severity score descend more significantly in CHDI than oseltamivir on day 2, and there were no differences on other symptoms. Conclusions. CHDI have a similar effect to oseltamivir in reducing the duration of influenza illness. CHDI was well tolerated, with no serious adverse events noted during the study period.
    Keywords Other systems of medicine ; RZ201-999
    Subject code 630
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark®) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens

    John Winslow / Gordon Parry / Jodi Weidler / Michael Bates / Thomas Sherwood / Shannon Utter / Weidong Huang / Linda D. B. Kiss / Sarah Irwin / Yining Shi / Judy Cheung / Lili Chen / Jolly Bose / Kristi Frankson / Amber Rivera / Jennifer W. Cook / Agnes C. Paquet / Lisa Defazio-Eli / Yuping Tan /
    Laurie J. Goodman / Jeffrey S. Larson / Christos J. Petropoulos / Jeannette M. Whitcomb

    Pathology Research International, Vol

    2010  Volume 2010

    Keywords Pathology ; RB1-214 ; Medicine ; R ; DOAJ:Pathology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2010-01-01T00:00:00Z
    Publisher Hindawi Publishing Corporation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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