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  1. Article ; Online: Antioxidants restore store‐operated Ca2+ entry in patient‐iPSC‐derived myotubes with tubular aggregate myopathy‐associated Ile484ArgfsX21 STIM1 mutation via upregulation of binding immunoglobulin protein

    Fusako Sakai‐Takemura / Fumiaki Saito / Ken'ichiro Nogami / Yusuke Maruyama / Ahmed Elhussieny / Kiichiro Matsumura / Shin'ichi Takeda / Yoshitsugu Aoki / Yuko Miyagoe‐Suzuki

    FASEB BioAdvances, Vol 5, Iss 11, Pp 453-

    2023  Volume 469

    Abstract: Abstract Store‐operated Ca2+ entry (SOCE) is indispensable for intracellular Ca2+ homeostasis in skeletal muscle, and constitutive activation of SOCE causes tubular aggregate myopathy (TAM). To understand the pathogenesis of TAM, we induced pluripotent ... ...

    Abstract Abstract Store‐operated Ca2+ entry (SOCE) is indispensable for intracellular Ca2+ homeostasis in skeletal muscle, and constitutive activation of SOCE causes tubular aggregate myopathy (TAM). To understand the pathogenesis of TAM, we induced pluripotent stem cells (iPSCs) from a TAM patient with a rare mutation (c.1450_1451insGA; p. Ile484ArgfsX21) in the STIM1 gene. This frameshift mutation produces a truncated STIM1 with a disrupted C‐terminal inhibitory domain (CTID) and was reported to diminish SOCE. Myotubes induced from the patient's‐iPSCs (TAM myotubes) showed severely impaired SOCE, but antioxidants greatly restored SOCE partly via upregulation of an endoplasmic reticulum (ER) chaperone, BiP (GRP78), in the TAM myotubes. Our observation suggests that antioxidants are promising tools for treatment of TAM caused by reduced SOCE.
    Keywords antioxidants ; binding immunoglobulin protein ; calcium ; calcium release‐activated calcium channel protein 1 (ORAI1) ; induced pluripotent stem cells ; skeletal muscle ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Large-scale integration in tablet screens for blue-light reduction with optimized color

    Masahiko Ayaki / Atsuhiko Hattori / Yusuke Maruyama / Kazuo Tsubota / Kazuno Negishi

    Cogent Biology, Vol 3, Iss

    The effects on sleep, sleepiness, and ocular parameters

    2017  Volume 1

    Abstract: We investigated sleep quality and visual symptoms in 30 adults who spent two hours before bedtime using a tablet device with and without the advanced technology of large-scale integration for blue-light reduction and color management. Dry eye- and eye ... ...

    Abstract We investigated sleep quality and visual symptoms in 30 adults who spent two hours before bedtime using a tablet device with and without the advanced technology of large-scale integration for blue-light reduction and color management. Dry eye- and eye fatigue-related symptom scores were significantly better with than without blue-light reduction. Sleepiness and saliva melatonin during the task were greater with blue-light reduction, however, overnight melatonin secretion and sleep quality parameters were similar in both conditions. In conclusion, tablet devices using large-scale integration for blue-light reduction increased sleepiness and reduced eye fatigue and dryness during tasks before bedtime.
    Keywords blue light ; sleep ; vision ; dry eye ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Prostaglandin EP2 receptor downstream of Notch signaling inhibits differentiation of human skeletal muscle progenitors in differentiation conditions

    Fusako Sakai-Takemura / Ken’ichiro Nogami / Ahmed Elhussieny / Kota Kawabata / Yusuke Maruyama / Naohiro Hashimoto / Shin’ichi Takeda / Yuko Miyagoe-Suzuki

    Communications Biology, Vol 3, Iss 1, Pp 1-

    2020  Volume 13

    Abstract: Abstract Understanding the signaling pathways that regulate proliferation and differentiation of muscle progenitors is essential for successful cell transplantation for treatment of Duchenne muscular dystrophy. Here, we report that a γ-secretase ... ...

    Abstract Abstract Understanding the signaling pathways that regulate proliferation and differentiation of muscle progenitors is essential for successful cell transplantation for treatment of Duchenne muscular dystrophy. Here, we report that a γ-secretase inhibitor, DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine tertial butyl ester), which inhibits the release of NICD (Notch intercellular domain), promotes the fusion of human muscle progenitors in vitro and improves their engraftment in the tibialis anterior muscle of immune-deficient mice. Gene expression analysis revealed that DAPT severely down-regulates PTGER2, which encodes prostaglandin (PG) E2 receptor 2 (EP2), in human muscle progenitors in the differentiation condition. Functional analysis suggested that Notch signaling inhibits differentiation and promotes self-renewal of human muscle progenitors via PGE2/EP2 signaling in a cAMP/PKA-independent manner.
    Keywords Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Mesenchymal stem cells derived from human induced pluripotent stem cells improve the engraftment of myogenic cells by secreting urokinase-type plasminogen activator receptor (uPAR)

    Ahmed Elhussieny / Ken’ichiro Nogami / Fusako Sakai-Takemura / Yusuke Maruyama / Natsumi Takemura / Wael Talaat Soliman / Shin’ichi Takeda / Yuko Miyagoe-Suzuki

    Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-

    2021  Volume 16

    Abstract: Abstract Background Duchenne muscular dystrophy (DMD) is a severe X-linked recessive disease caused by mutations in the dystrophin gene. Transplantation of myogenic stem cells holds great promise for treating muscular dystrophies. However, poor ... ...

    Abstract Abstract Background Duchenne muscular dystrophy (DMD) is a severe X-linked recessive disease caused by mutations in the dystrophin gene. Transplantation of myogenic stem cells holds great promise for treating muscular dystrophies. However, poor engraftment of myogenic stem cells limits the therapeutic effects of cell therapy. Mesenchymal stem cells (MSCs) have been reported to secrete soluble factors necessary for skeletal muscle growth and regeneration. Methods We induced MSC-like cells (iMSCs) from induced pluripotent stem cells (iPSCs) and examined the effects of iMSCs on the proliferation and differentiation of human myogenic cells and on the engraftment of human myogenic cells in the tibialis anterior (TA) muscle of NSG-mdx 4Cv mice, an immunodeficient dystrophin-deficient DMD model. We also examined the cytokines secreted by iMSCs and tested their effects on the engraftment of human myogenic cells. Results iMSCs promoted the proliferation and differentiation of human myogenic cells to the same extent as bone marrow-derived (BM)-MSCs in coculture experiments. In cell transplantation experiments, iMSCs significantly improved the engraftment of human myogenic cells injected into the TA muscle of NSG-mdx 4Cv mice. Cytokine array analysis revealed that iMSCs produced insulin-like growth factor-binding protein 2 (IGFBP2), urokinase-type plasminogen activator receptor (uPAR), and brain-derived neurotrophic factor (BDNF) at higher levels than did BM-MSCs. We further found that uPAR stimulates the migration of human myogenic cells in vitro and promotes their engraftment into the TA muscles of immunodeficient NOD/Scid mice. Conclusions Our results indicate that iMSCs are a new tool to improve the engraftment of myogenic progenitors in dystrophic muscle.
    Keywords Human iPS cells ; Mesenchymal stem cells ; Skeletal muscle ; Muscle progenitors ; Cell transplantation ; Duchenne muscular dystrophy (DMD) ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
    Subject code 610
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Kynurenine promotes Calcitonin secretion and reduces cortisol in the Japanese flounder Paralichthys olivaceus

    Takahiro Ikari / Yukihiro Furusawa / Yoshiaki Tabuchi / Yusuke Maruyama / Atsuhiko Hattori / Yoichiro Kitani / Kenji Toyota / Arata Nagami / Jun Hirayama / Kazuki Watanabe / Atsushi Shigematsu / Muhammad Ahya Rafiuddin / Shouzo Ogiso / Keisuke Fukushi / Kohei Kuroda / Kaito Hatano / Toshio Sekiguchi / Ryotaro Kawashima / Ajai K. Srivastav /
    Takumi Nishiuchi / Akihiro Sakatoku / Masa-aki Yoshida / Hajime Matsubara / Nobuo Suzuki

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 12

    Abstract: Abstract Deep ocean water (DOW) exerts positive effects on the growth of marine organisms, suggesting the presence of unknown component(s) that facilitate their aquaculture. We observed that DOW suppressed plasma cortisol (i.e., a stress marker) ... ...

    Abstract Abstract Deep ocean water (DOW) exerts positive effects on the growth of marine organisms, suggesting the presence of unknown component(s) that facilitate their aquaculture. We observed that DOW suppressed plasma cortisol (i.e., a stress marker) concentration in Japanese flounder (Paralichthys olivaceus) reared under high-density condition. RNA-sequencing analysis of flounder brains showed that when compared to surface seawater (SSW)-reared fish, DOW-reared fish had lower expression of hypothalamic (i.e., corticotropin-releasing hormone) and pituitary (i.e., proopiomelanocortin, including adrenocorticotropic hormone) hormone-encoding genes. Moreover, DOW-mediated regulation of gene expression was linked to decreased blood cortisol concentration in DOW-reared fish. Our results indicate that DOW activated osteoblasts in fish scales and facilitated the production of Calcitonin, a hypocalcemic hormone that acts as an analgesic. We then provide evidence that the Calcitonin produced is involved in the regulatory network of genes controlling cortisol secretion. In addition, the indole component kynurenine was identified as the component responsible for osteoblast activation in DOW. Furthermore, kynurenine increased plasma Calcitonin concentrations in flounders reared under high-density condition, while it decreased plasma cortisol concentration. Taken together, we propose that kynurenine in DOW exerts a cortisol-reducing effect in flounders by facilitating Calcitonin production by osteoblasts in the scales.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish

    Kase, Yoichi / Akira Kambegawa / Atsuhiko Hattori / Hisayuki Funahashi / Honoo Satake / Kei-ichiro Kitamura / Masato Endo / Masayuki Sato / Nobuo Suzuki / Shin Matsubara / Shouzo Ogiso / Takahiro Ikari / Takushi X. Watanabe / Toshio Sekiguchi / Tsuyoshi Kawada / Yoshinari Watanabe / Yuichi Sasayama / Yusuke Maruyama

    Comparative biochemistry and physiology. 2017 Sept., v. 211

    2017  

    Abstract: The nucleotide sequence of a sardine preprocalcitonin precursor has been determined from their ultimobranchial glands in the present study. From our analysis of this sequence, we found that sardine procalcitonin was composed of procalcitonin amino- ... ...

    Abstract The nucleotide sequence of a sardine preprocalcitonin precursor has been determined from their ultimobranchial glands in the present study. From our analysis of this sequence, we found that sardine procalcitonin was composed of procalcitonin amino-terminal cleavage peptide (N-proCT) (53 amino acids), CT (32 amino acids), and procalcitonin carboxyl-terminal cleavage peptide (C-proCT) (18 amino acids). As compared with C-proCT, N-proCT has been highly conserved among teleosts, reptiles, and birds, which suggests that N-proCT has some bioactivities. Therefore, both sardine N-proCT and sardine CT were synthesized, and their bioactivities for osteoblasts and osteoclasts were examined using our assay system with goldfish scales that consisted of osteoblasts and osteoclasts. As a result, sardine N-proCT (10−7M) activated osteoblastic marker enzyme activity, while sardine CT did not change. On the other hand, sardine CT (10−9 to 10−7M) suppressed osteoclastic marker enzyme activity, although sardine N-proCT did not influence enzyme activity. Furthermore, the mRNA expressions of osteoblastic markers such as type 1 collagen and osteocalcin were also promoted by sardine N-proCT (10−7M) treatment; however, sardine CT did not influence their expressions. The osteoblastic effects of N-proCT lack agreement. In the present study, we can evaluate exactly the action for osteoblasts because our scale assay system is very sensitive and it is a co-culture system for osteoblasts and osteoclasts with calcified bone matrix. Both CT and N-proCT seem to influence osteoblasts and osteoclasts and promote bone formation by different actions in teleosts.
    Keywords amino acids ; bioactive properties ; birds ; bone formation ; calcitonin ; coculture ; collagen ; enzyme activity ; goldfish ; messenger RNA ; nucleotide sequences ; osteoblasts ; osteocalcin ; osteoclasts ; reptiles ; sardines
    Language English
    Dates of publication 2017-09
    Size p. 77-83.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 121246-1
    ISSN 1531-4332 ; 0300-9629 ; 1095-6433
    ISSN (online) 1531-4332
    ISSN 0300-9629 ; 1095-6433
    DOI 10.1016/j.cbpa.2017.06.007
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology.

    Aya Obana-Koshino / Hitomi Ono / Jiro Miura / Manabu Sakai / Hitoshi Uchida / Wataru Nakamura / Kanji Nohara / Yusuke Maruyama / Atsuhiko Hattori / Takayoshi Sakai

    PLoS ONE, Vol 10, Iss 4, p e

    2015  Volume 0119960

    Abstract: Many organs, including salivary glands, lung, and kidney, are formed by epithelial branching during embryonic development. Branching morphogenesis occurs via either local outgrowths or the formation of clefts that subdivide epithelia into buds. This ... ...

    Abstract Many organs, including salivary glands, lung, and kidney, are formed by epithelial branching during embryonic development. Branching morphogenesis occurs via either local outgrowths or the formation of clefts that subdivide epithelia into buds. This process is promoted by various factors, but the mechanism of branching morphogenesis is not fully understood. Here we have defined melatonin as a potential negative regulator or "brake" of branching morphogenesis, shown that the levels of it and its receptors decline when branching morphogenesis begins, and identified the process that it regulates. Melatonin has various physiological functions, including circadian rhythm regulation, free-radical scavenging, and gonadal development. Furthermore, melatonin is present in saliva and may have an important physiological role in the oral cavity. In this study, we found that the melatonin receptor is highly expressed on the acinar epithelium of the embryonic submandibular gland. We also found that exogenous melatonin reduces salivary gland size and inhibits branching morphogenesis. We suggest that this inhibition does not depend on changes in either proliferation or apoptosis, but rather relates to changes in epithelial cell adhesion and morphology. In summary, we have demonstrated a novel function of melatonin in organ formation during embryonic development.
    Keywords Medicine ; R ; Science ; Q
    Subject code 571
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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