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  1. Article ; Online: Corrigendum

    Yves Henrotin / Zoltan Szekanecz / Kayo Masuko

    Frontiers in Medicine, Vol

    Editorial: Reviews in rheumatology

    2023  Volume 10

    Keywords rheumatoid arthritis ; systemic lupus erythematosus (SLE) ; frozen shoulder ; cytokines ; biomarkers ; mitochondria ; Medicine (General) ; R5-920
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Editorial

    Yves Henrotin / Zoltan Szekanecz / Kayo Masuko

    Frontiers in Medicine, Vol

    Reviews in rheumatology

    2023  Volume 10

    Keywords rheumatoid arthritis ; systemic lupus erythematosus (SLE) ; frozen shoulder ; cytokines ; biomarkers ; mitochondria ; Medicine (General) ; R5-920
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Interleukins, growth factors, and transcription factors are key targets for gene therapy in osteoarthritis

    Melanie Uebelhoer / Cécile Lambert / Juliane Grisart / Kilian Guse / Stanislav Plutizki / Yves Henrotin

    Frontiers in Medicine, Vol

    A scoping review

    2023  Volume 10

    Abstract: ObjectiveOsteoarthritis (OA) is the most common degenerative joint disease, characterized by a progressive loss of cartilage associated with synovitis and subchondral bone remodeling. There is however no treatment to cure or delay the progression of OA. ... ...

    Abstract ObjectiveOsteoarthritis (OA) is the most common degenerative joint disease, characterized by a progressive loss of cartilage associated with synovitis and subchondral bone remodeling. There is however no treatment to cure or delay the progression of OA. The objective of this manuscript was to provide a scoping review of the preclinical and clinical studies reporting the effect of gene therapies for OA.MethodThis review followed the JBI methodology and was reported in accordance with the PRISMA-ScR checklist. All research studies that explore in vitro, in vivo, or ex vivo gene therapies that follow a viral or non-viral gene therapy approach were considered. Only studies published in English were included in this review. There were no limitations to their date of publication, country of origin, or setting. Relevant publications were searched in Medline ALL (Ovid), Embase (Elsevier), and Scopus (Elsevier) in March 2023. Study selection and data charting were performed by two independent reviewers.ResultsWe found a total of 29 different targets for OA gene therapy, including studies examining interleukins, growth factors and receptors, transcription factors and other key targets. Most articles were on preclinical in vitro studies (32 articles) or in vivo animal models (39 articles), while four articles were on clinical trials related to the development of TissueGene-C (TG-C).ConclusionIn the absence of any DMOAD, gene therapy could be a highly promising treatment for OA, even though further development is required to bring more targets to the clinical stage.
    Keywords genetic therapy ; osteoarthritis ; gene transfer techniques ; interleukins ; growth factors ; transcription factors ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2023-04-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Identification of new biomarkers for sarcopenia and characterization of cathepsin D biomarker

    Corine L'hôte / Benoît Cordier / Alain Labasse / Christelle Boileau / Bérénice Costes / Yves Henrotin

    JCSM Rapid Communications, Vol 4, Iss 2, Pp 122-

    2021  Volume 132

    Abstract: Abstract Background Sarcopenia is the progressive generalized loss of skeletal muscle mass, strength, and function that occurs with aging. This study was undertaken to identify new biomarkers of sarcopenia by proteomics analysis of female sera. Methods A ...

    Abstract Abstract Background Sarcopenia is the progressive generalized loss of skeletal muscle mass, strength, and function that occurs with aging. This study was undertaken to identify new biomarkers of sarcopenia by proteomics analysis of female sera. Methods A case–control study was set up, for which 19 sarcopenic subjects and 20 control subjects, according to the European Working Group on Sarcopenia Older People criteria published in 2010 (EWGSOP1), were enrolled. All the subjects were at least 65 years old and in majority female. Biomarker screening was performed by a comparative mass spectrometry analysis. Protein expression levels between the two groups were compared. One of the identified biomarkers, cathepsin D, was measured by immunoassay on the serum of the full sample set (n = 39). Its diagnostic performance was evaluated with a receiver operating characteristic curve (ROC curve). Results Two biomarkers were identified: fructose‐biphosphate aldolase A (P ≤ 0.05) and cathepsin D (P ≤ 0.05). The levels of all of them were higher in sarcopenic patients. It was confirmed by immunoassay that cathepsin D levels in serum were significantly higher in the sarcopenic group of patients (P = 0.038). An inverse correlation (−0.385) was observed between cathepsin D levels in serum and gait speed. The area under the ROC curve measurement (AUC = 0.696) demonstrated that cathepsin D levels could discriminate between sarcopenic and non‐sarcopenic subjects. A predictive model including cathepsin D, age, and body mass index was established to improve the diagnostic performance (AUC = 0.908). Conclusions Cathepsin D has been identified as a diagnostic biomarker of sarcopenia.
    Keywords Sarcopenia ; Proteomics ; Cathepsin D ; Muscle ; Diagnosis ; Biomarker ; Internal medicine ; RC31-1245
    Subject code 796
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: The Damage-Associated Molecular Patterns (DAMPs) as Potential Targets to Treat Osteoarthritis

    Cécile Lambert / Jérémie Zappia / Christelle Sanchez / Antoine Florin / Jean-Emile Dubuc / Yves Henrotin

    Frontiers in Medicine, Vol

    Perspectives From a Review of the Literature

    2021  Volume 7

    Abstract: During the osteoarthritis (OA) process, activation of immune systems, whether innate or adaptive, is strongly associated with low-grade systemic inflammation. This process is initiated and driven in the synovial membrane, especially by synovium cells, ... ...

    Abstract During the osteoarthritis (OA) process, activation of immune systems, whether innate or adaptive, is strongly associated with low-grade systemic inflammation. This process is initiated and driven in the synovial membrane, especially by synovium cells, themselves previously activated by damage-associated molecular patterns (DAMPs) released during cartilage degradation. These fragments exert their biological activities through pattern recognition receptors (PRRs) that, as a consequence, induce the activation of signaling pathways and beyond the release of inflammatory mediators, the latter contributing to the vicious cycle between cartilage and synovial membrane. The primary endpoint of this review is to provide the reader with an overview of these many molecules categorized as DAMPs and the contribution of the latter to the pathophysiology of OA. We will also discuss the different strategies to control their effects. We are convinced that a better understanding of DAMPs, their receptors, and associated pathological mechanisms represents a decisive issue for degenerative joint diseases such as OA.
    Keywords osteoarthritis ; cartilage ; immunity ; inflammation ; synovitis ; Medicine (General) ; R5-920
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: A Zebrafish Mutant in the Extracellular Matrix Protein Gene efemp1 as a Model for Spinal Osteoarthritis

    Ratish Raman / Mohamed Ali Bahri / Christian Degueldre / Caroline Caetano da Silva / Christelle Sanchez / Agnes Ostertag / Corinne Collet / Martine Cohen-Solal / Alain Plenevaux / Yves Henrotin / Marc Muller

    Animals, Vol 14, Iss 1, p

    2023  Volume 74

    Abstract: Osteoarthritis is a degenerative articular disease affecting mainly aging animals and people. The extracellular matrix protein Efemp1 was previously shown to have higher turn-over and increased secretion in the blood serum, urine, and subchondral bone of ...

    Abstract Osteoarthritis is a degenerative articular disease affecting mainly aging animals and people. The extracellular matrix protein Efemp1 was previously shown to have higher turn-over and increased secretion in the blood serum, urine, and subchondral bone of knee joints in osteoarthritic patients. Here, we use the zebrafish as a model system to investigate the function of Efemp1 in vertebrate skeletal development and homeostasis. Using in situ hybridization, we show that the efemp1 gene is expressed in the brain, the pharyngeal arches, and in the chordoblasts surrounding the notochord at 48 hours post-fertilization. We generated an efemp1 mutant line, using the CRISPR/Cas9 method, that produces a severely truncated Efemp1 protein. These mutant larvae presented a medially narrower chondrocranium at 5 days, which normalized later at day 10. At age 1.5 years, µCT analysis revealed an increased tissue mineral density and thickness of the vertebral bodies, as well as a decreased distance between individual vertebrae and ruffled borders of the vertebral centra. This novel defect, which has, to our knowledge, never been described before, suggests that the efemp1 mutant represents the first zebrafish model for spinal osteoarthritis.
    Keywords zebrafish ; skeletal development ; ECM ; efemp1 ; notochord ; vertebra ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 612
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Editorial

    Ali Mobasheri / João Eurico Fonseca / Oreste Gualillo / Yves Henrotin / Raquel Largo / Gabriel Herrero-Beaumont / Francisco Airton Castro Rocha

    Frontiers in Medicine, Vol

    Inflammation and Biomarkers in Osteoarthritis

    2021  Volume 8

    Keywords osteoarthritis ; biomarkers ; cartilage ; synovium ; inflammation ; Medicine (General) ; R5-920
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Oral supplementation with fish cartilage hydrolysate in an adult population suffering from knee pain and function discomfort: results from an innovative approach combining an exploratory clinical study and an ex vivo clinical investigation.

    Yves, Henrotin / Herman, Julie / Uebelhoer, Melanie / Wauquier, Fabien / Boutin-Wittrant, Line / Donneau, Anne-Françoise / Monseur, Justine / Fotso, Variance Mokam / Duquenne, Marie / Wagner, Mélanie / Bouvret, Elodie / Costes, Bérénice / Wittrant, Yohann

    BMC musculoskeletal disorders

    2023  Volume 24, Issue 1, Page(s) 748

    Abstract: Background: Aging is frequently associated with impairments of the musculoskeletal system and many elderly people experience joint discomfort or pain which might reduce their ability to move and consequently alter their quality of life. A beneficial ... ...

    Abstract Background: Aging is frequently associated with impairments of the musculoskeletal system and many elderly people experience joint discomfort or pain which might reduce their ability to move and consequently alter their quality of life. A beneficial effect of fish cartilage hydrolysate (FCH) on pain and joint function has recently been shown in an ACLT/pMMx osteoarthritis rat model.
    Methods: We therefore performed an exploratory, non-comparative, multi-centric clinical trial including 33 subjects with moderate knee joint discomfort and loss of functionality to investigate the efficacy of FCH on their algo-functional status. We further determined the potential health benefit of FCH in an original clinical ex vivo study investigating the role of FCH human metabolites on primary human chondrocytes.
    Results: FCH significantly improved knee pain and function, as assessed by the Knee injury and Osteoarthritis Outcome Score (KOOS). Moreover, FCH significantly reduced pain at rest and while walking, and patient global assessment (PGA), as assessed by the Visual Analogue Scale (VAS), and improved patients' quality of life (SF-36). FCH metabolites decreased the synthesis of catabolic factors (MMP-13) and pro-inflammatory mediators (NO, PGE2) and limited the inhibitory effect of IL-1β on the synthesis of cartilage matrix components (GAG and collagen).
    Conclusions: Thus, these data provide insights on the mode of action of FCH in humans and contribute to explain how FCH may relieve pain and improve joint function in subjects with knee discomfort. Although these preliminary data need to be confirmed in a randomized controlled trial, they strongly support the potential health benefit of such an active ingredient.
    Trial registration: The study was registered on clinicaltrials.gov with the identifier NCT04420091 (09/06/2020).
    MeSH term(s) Aged ; Humans ; Adult ; Animals ; Rats ; Quality of Life ; Knee Joint ; Cartilage ; Osteoarthritis ; Pain ; Dietary Supplements
    Language English
    Publishing date 2023-09-21
    Publishing country England
    Document type Randomized Controlled Trial ; Journal Article
    ZDB-ID 2041355-5
    ISSN 1471-2474 ; 1471-2474
    ISSN (online) 1471-2474
    ISSN 1471-2474
    DOI 10.1186/s12891-023-06800-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Application for proteomic techniques in studying osteoarthritis

    YvesHenrotin / MyriamGharbi

    Frontiers in Physiology, Vol

    a review

    2011  Volume 2

    Abstract: After the genomic era, proteomic corresponds to a wide variety of techniques to study the protein content of cells, tissue or organism and to isolate protein of interest. It offers the choice between gel-based and gel-free methods or shotgun proteomics. ... ...

    Abstract After the genomic era, proteomic corresponds to a wide variety of techniques to study the protein content of cells, tissue or organism and to isolate protein of interest. It offers the choice between gel-based and gel-free methods or shotgun proteomics. Applications of proteomic technology may concern three principal objectives in several biomedical or clinical domains of research as in osteoarthritis: (i) to understand the physiopathology and underlying mechanisms leading to a disease or associated to a particular model, (ii), to find disease-specific biomarker and (iii) to identify new therapeutic targets. This review aimed at gathering most of the data regarding the proteomic techniques and their applications to arthritis research. It also reported technical limitations and solutions, as for example for sample preparation. Proteomics open wide perspectives in biochemical research but many technical matters still remain to be solved.
    Keywords Osteoarthritis ; proteomic ; Physiology ; QP1-981 ; Science ; Q
    Language English
    Publishing date 2011-12-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: REG-O3 chimeric peptide combining growth hormone and somatostatin sequences improves joint function and prevents cartilage degradation in rat model of traumatic knee osteoarthritis.

    Rodrick Montjean / Sonia Escaich / Raffaello Paolini / Claude Carelli / Sébastien Pirson / Thibaut Neutelings / Yves Henrotin / Christelle Vêtu

    PLoS ONE, Vol 15, Iss 4, p e

    2020  Volume 0231240

    Abstract: OBJECTIVE:REG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormone (GH) and an analog of somatostatin (SST), molecules displaying cartilage repair and anti-inflammatory properties, respectively. This study aimed to ... ...

    Abstract OBJECTIVE:REG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormone (GH) and an analog of somatostatin (SST), molecules displaying cartilage repair and anti-inflammatory properties, respectively. This study aimed to investigate the disease-modifying osteoarthritis drug (DMOAD) potential of REG-O3 by analyzing its effect on pain, joint function and structure, upon injection into osteoarthritic rat knee joint. DESIGN:Osteoarthritis was induced in the right knee of mature male Lewis rats (n = 12/group) by surgical transection of the anterior cruciate ligament (ACLT) combined with partial medial meniscectomy (pMMx). Treatments were administered intra-articularly from fourteen days after surgery through three consecutive injections one week apart. The effect of REG-O3, solubilized in a liposomal solution and injected at either 5, 25 or 50 μg/50 μL, was compared to liposomal (LIP), dexamethasone and hyaluronic acid (HA) solutions. The study endpoints were the pain/function measured once a week throughout the entire study, and the joint structure evaluated eight weeks after surgery using OARSI score. RESULTS:ACLT/pMMx surgery induced a significant modification of weight bearing in all groups. When compared to liposomal solution, REG-O3 was able to significantly improve weight bearing as efficiently as dexamethasone and HA. REG-O3 (25 μg) was also able to significantly decrease OARSI histological global score as well as degeneration of both cartilage and matrix while the other treatments did not. CONCLUSION:This study provides evidence of a remarkable protecting effect of REG-O3 on pain/knee joint function and cartilage/matrix degradation in ACLT/pMMx model of rat osteoarthritis. REG-O3 thus displays an interesting profile as a DMOAD.
    Keywords Medicine ; R ; Science ; Q
    Subject code 796
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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