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  1. Article ; Online: Zirconia-Toughened Alumina Ceramic Wear Particles Do Not Elicit Inflammatory Responses in Human Macrophages

    Alessandro Alan Porporati / Yvonne Mödinger / Sarah Fischer / Sara Polajžer / Melanie Mettang / Ulrike Deisinger / Matejka Podlogar / Rihard Trebše / Nika Lovšin

    International Journal of Molecular Sciences, Vol 24, Iss 6482, p

    2023  Volume 6482

    Abstract: Ten percent of patients undergoing total hip arthroplasty (THA) require revision surgery. One of the reasons for THA are wear particles released from the implants that can activate the immune defense and cause osteolysis and failure of the joint implant. ...

    Abstract Ten percent of patients undergoing total hip arthroplasty (THA) require revision surgery. One of the reasons for THA are wear particles released from the implants that can activate the immune defense and cause osteolysis and failure of the joint implant. The discrepancies between reports on toxicity and immunogenicity of the implant materials led us to this study in which we compared toxicity and immunogenicity of well-defined nanoparticles from Al 2 O 3 , zirconia-toughened alumina (ZTA), and cobalt chrome (CoCr), a human THP-1 macrophage cell line, human PBMCs, and therefrom-derived primary macrophages. None of the tested materials decreased the viability of THP-1 macrophages nor human primary macrophages at the 24 h time point, indicating that at concentrations from 0.05 to 50 µm 3 /cell the tested materials are non-toxic. Forty-eight hours of treatment of THP-1 macrophages with 5 µm 3 /cell of CoCr and Al 2 O 3 caused 8.3-fold and 4.6-fold increases in TNF-α excretion, respectively, which was not observed for ZTA. The comparison between THP-1 macrophages and human primary macrophages revealed that THP-1 macrophages show higher activation of cytokine expression in the presence of CoCr and Al 2 O 3 particles than primary macrophages. Our results indicate that ZTA is a non-toxic implant material with no immunogenic effects in vitro.
    Keywords orthopaedic implant ; alumina ; zirconia-toughened alumina (ZTA) ; nanoparticles ; macrophages ; cell viability ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 616
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: A new high-quality elderberry plant extract exerts antiviral and immunomodulatory effects in vitro and ex vivo

    Christiane Schön / Yvonne Mödinger / Franziska Krüger / Cornelia Doebis / Ivo Pischel / Bernd Bonnländer

    Food and Agricultural Immunology, Vol 32, Iss 1, Pp 650-

    2021  Volume 662

    Abstract: Infections of the respiratory system, including common cold and influenza, are affecting people worldwide and are more or less prone to spread depending on the season and viral load of the host. For reducing symptoms and duration of illness, treatment ... ...

    Abstract Infections of the respiratory system, including common cold and influenza, are affecting people worldwide and are more or less prone to spread depending on the season and viral load of the host. For reducing symptoms and duration of illness, treatment options to standard prescribed drugs are in demand. Natural products could provide immune-supporting treatment alternatives. Elderberry extracts have been used in traditional medicine for the treatment of respiratory infections for decades and numerous studies describe the beneficial effects of elderberries on the immune system and respiratory infectious disease. We investigated the immunomodulative and antiviral effects of a high-quality, anthocyanin-enriched elderberry fruit extract (eldosamb®). Results reveal that elderberry extract reduced the secretion of pro-inflammatory cytokines TNF-α and IFN-γ, leading to a shift towards the Th2-Helper cell response and showing antiviral efficacy against the MVA virus. Thus, with its anti-inflammatory and antiviral bioactivity the proprietary elderberry extract suggests its use as an immunomodulatory health product.
    Keywords elderberry (sambucus nigra l.) extract ; tnf-α ; inflammation ; t-cell activation ; modified vaccinia virus ankara ; il-10 ; Agriculture (General) ; S1-972 ; Immunologic diseases. Allergy ; RC581-607
    Subject code 610
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Enhanced Oral Bioavailability of β-Caryophyllene in Healthy Subjects Using the VESIsorb ® Formulation Technology, a Novel Self-Emulsifying Drug Delivery System (SEDDS)

    Yvonne Mödinger / Katharina Knaub / Tanita Dharsono / Roland Wacker / Remo Meyrat / M. Hunter Land / Anthony L. Petraglia / Christiane Schön

    Molecules, Vol 27, Iss 2860, p

    2022  Volume 2860

    Abstract: β-Caryophyllene (BCP), a common constituent of many spice and food plants, is gaining increased attention due to recent research identifying numerous potential health benefits. Due to limited oral bioavailability observed in preclinical models, the ... ...

    Abstract β-Caryophyllene (BCP), a common constituent of many spice and food plants, is gaining increased attention due to recent research identifying numerous potential health benefits. Due to limited oral bioavailability observed in preclinical models, the described benefits of BCP may be maximized by using a suitable delivery system. Additionally, human pharmacokinetics (PK) remain unknown. This study evaluates the relative oral bioavailability of BCP formulated in a self-emulsifying drug delivery system (SEDDS) based on VESIsorb ® formulation technology (BCP-SEDDS) compared to BCP neat oil. Hence, a randomized, double-blind, cross-over design, single oral dose study (100 mg BCP) in 24 healthy subjects (12 men/12 women) was performed under fasting conditions. Pharmacokinetic parameters were analyzed from individual concentration-time curves. The data show that BCP-SEDDS resulted in a 2.2/2.0-fold increase in AUC 0–12h /AUC 0–24h and a 3.6-fold increase in C max compared to BCP neat oil. Moreover, BCP was absorbed faster from BCP-SEDDS (T max : 1.43 h) compared to BCP neat oil (T max : 3.07 h). Gender analysis revealed that there is no significant difference between men and women for both the investigated formulations and all investigated PK endpoints. In conclusion, BCP-SEDDS offers a well-tolerated and effective oral delivery system to significantly enhance the oral bioavailability of BCP in humans.
    Keywords beta-caryophyllene (BCP) ; bioavailability ; hemp ; Cannabis sativa L ; human ; oral drug delivery system ; Organic chemistry ; QD241-441
    Subject code 610
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Increased Presence of Complement Factors and Mast Cells in Alveolar Bone and Tooth Resorption

    Kathrin Luntzer / Ina Lackner / Birte Weber / Yvonne Mödinger / Anita Ignatius / Florian Gebhard / Susann-Yvonne Mihaljevic / Melanie Haffner-Luntzer / Miriam Kalbitz

    International Journal of Molecular Sciences, Vol 22, Iss 2759, p

    2021  Volume 2759

    Abstract: Periodontitis is the inflammatory destruction of the tooth-surrounding and -supporting tissue, resulting at worst in tooth loss. Another locally aggressive disease of the oral cavity is tooth resorption (TR). This is associated with the destruction of ... ...

    Abstract Periodontitis is the inflammatory destruction of the tooth-surrounding and -supporting tissue, resulting at worst in tooth loss. Another locally aggressive disease of the oral cavity is tooth resorption (TR). This is associated with the destruction of the dental mineralized tissue. However, the underlying pathomechanisms remain unknown. The complement system, as well as mast cells (MCs), are known to be involved in osteoclastogenesis and bone loss. The complement factors C3 and C5 were previously identified as key players in periodontal disease. Therefore, we hypothesize that complement factors and MCs might play a role in alveolar bone and tooth resorption. To investigate this, we used the cat as a model because of the naturally occurring high prevalence of both these disorders in this species. Teeth, gingiva samples and serum were collected from domestic cats, which had an appointment for dental treatment under anesthesia, as well as from healthy cats. Histological analyses, immunohistochemical staining and the CH-50 and AH-50 assays revealed increased numbers of osteoclasts and MCs, as well as complement activity in cats with TR. Calcifications score in the gingiva was highest in animals that suffer from TR. This indicates that MCs and the complement system are involved in the destruction of the mineralized tissue in this condition.
    Keywords tooth resorption ; periodontitis ; mast cells ; complement system ; osteoclasts ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 616
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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