Article ; Online: Evaluation of a dilution method for non-evaluable results in the detection of Chlamydia trachomatis and Neisseria gonorrhoeae with the Cobas 4800 platform.
Enfermedades infecciosas y microbiologia clinica
2017 Volume 35, Issue 6, Page(s) 364–366
Abstract: Introduction: A variable percentage of samples analysed using the Cobas 4800 assay can give an invalid result by PCR inhibition or erroneous due to incorrect DNA extraction with the Cobas 4800 CT/NG test.: Method: An analysis was performed using the ... ...
Title translation | Valoración de un método de dilución para la resolución de resultados no valorables en la detección de Chlamydia trachomatis y Neisseria gonorrhoeae con la plataforma cobas 4800. |
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Abstract | Introduction: A variable percentage of samples analysed using the Cobas 4800 assay can give an invalid result by PCR inhibition or erroneous due to incorrect DNA extraction with the Cobas 4800 CT/NG test. Method: An analysis was performed using the vortex agitation and dilution protocol on the original sample (swab or urine) for a total of 116 samples. In order to analyse the sensitivity of this method, 100 samples (swabs and urine) with known results were retested. Results: A total of 98.3% (114/116) of the samples analysed were resolved with this protocol with 100% agreement after reviewing clinical data, Gram stain, and other samples analysed in parallel from the same patient. Discussion: The data indicate no loss of sensitivity with this protocol; thus Cobas 4800 users could use this method without the need for alternative methods. |
Language | Spanish |
Publishing date | 2017-06 |
Publishing country | Spain |
Document type | Journal Article |
ZDB-ID | 1070941-1 |
ISSN | 1578-1852 ; 0213-005X |
ISSN (online) | 1578-1852 |
ISSN | 0213-005X |
DOI | 10.1016/j.eimc.2015.06.017 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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