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Article ; Online: [Evaluation of a new portable analyzer, HemoCue

Zamorano, Bryan / Sakr, Yolla / Galopin-Dubois, Frédérique

2024 Volume 82, Issue 1, Page(s) 103–111

Abstract: The use of portable hemoglobin measuring devices is widespread. In this context, the company HemoCue® has put on the market a new device, the Hb801. It uses a whole blood absorbance measurement method and not the azidmethemoglobin measurement method used ...

Title translation	Évaluation d’un nouvel automate de biologie délocalisée pour doser l’hémoglobine, l’HemoCue® Hb801.
Abstract	The use of portable hemoglobin measuring devices is widespread. In this context, the company HemoCue® has put on the market a new device, the Hb801. It uses a whole blood absorbance measurement method and not the azidmethemoglobin measurement method used by HemoCue's older devices. We evaluated this new equipment on EDTA venous blood. Hb801 is lightweight, compact, requires a volume of 10 μL of blood and renders its result in less than a second. The repeatability and intermediate precision are close to the values expected according to Ricos, with coefficients of variation respectively for a low level of hemoglobin: 2.1% and 1.9%, for an average level: 0.8% and 1.5% and for a high level: 1.5% and 1.3%. Comparison to our laboratory reference method (XN-10 Sysmex®) and HemoCue® Hb201+ was performed on 96 samples. Bias (SD) found were: XN-10: +0.42 g/dL (0.17), HemoCue® Hb201+: +0.17 g/dL (0.41). Clinically acceptable performance (within ± 1 g/dL of reference hemoglobin) was high: 93.8%. In the end, this device seems to us to be suitable for hemoglobin point-of-care testing.
MeSH term(s)	Humans ; Hemoglobinometry/methods ; Hemoglobins/analysis ; Point-of-Care Testing ; Point-of-Care Systems
Chemical Substances	Hemoglobins
Language	French
Publishing date	2024-04-19
Publishing country	France
Document type	English Abstract ; Journal Article
ZDB-ID	418098-7
ISSN	1950-6112 ; 0003-3898
ISSN (online)	1950-6112
ISSN	0003-3898
DOI	10.1684/abc.2024.1868
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Article ; Online: How Really Ancient Is Paulinella Chromatophora?

Delaye, Luis / Valadez-Cano, Cecilio / Pérez-Zamorano, Bernardo

PLoS currents

2016 Volume 8

Abstract: The ancestor of Paulinella chromatophora established a symbiotic relationship with cyanobacteria related to the Prochloroccocus/Synechococcus clade. This event has been described as a second primary endosymbiosis leading to a plastid in the making. Based ...

Abstract	The ancestor of Paulinella chromatophora established a symbiotic relationship with cyanobacteria related to the Prochloroccocus/Synechococcus clade. This event has been described as a second primary endosymbiosis leading to a plastid in the making. Based on the rate of pseudogene disintegration in the endosymbiotic bacteria Buchnera aphidicola, it was suggested that the chromatophore in P. chromatophora has a minimum age of ~60 Myr. Here we revisit this estimation by using a lognormal relaxed molecular clock on the 18S rRNA of P. chromatophora. Our time estimates show that depending on the assumptions made to calibrate the molecular clock, P. chromatophora diverged from heterotrophic Paulinella spp. ~ 90 to 140 Myr ago, thus establishing a maximum date for the origin of the chromatophore.
Language	English
Publishing date	2016-03-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2583641-9
ISSN	2157-3999 ; 2157-3999
ISSN (online)	2157-3999
ISSN	2157-3999
DOI	10.1371/currents.tol.e68a099364bb1a1e129a17b4e06b0c6b
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Article: Subcellular localization of prostaglandin-E2 in rat heart tissue.

Zamorano, B

Cardiovascular drugs and therapy

1991 Volume 5, Issue 3, Page(s) 655–657

Abstract: It has been reported that isolated rat heart myocytes and cardiac mesenchymal cells convert arachidonic acid mainly into three types of prostaglandins (PGs): PGE2, PGF2 alpha, and PGI2. In addition, we have demonstrated that fresh atrial slices of ... ...

Abstract	It has been reported that isolated rat heart myocytes and cardiac mesenchymal cells convert arachidonic acid mainly into three types of prostaglandins (PGs): PGE2, PGF2 alpha, and PGI2. In addition, we have demonstrated that fresh atrial slices of patients with heart-valve disease contain appreciable quantities of PGE2 and PGF2 alpha. However, there have been no reports on the subcellular localization of the prostaglandin system in heart muscle tissue. The present study was performed to define the distribution of PGE2 in mitochondrial, microsomal, and cytosolic fractions, isolated by differential centrifugation from homogenates of fresh normal rat atrium and ventricle slices. In addition, we determined whether differences exist in PGE2 levels between atrial subcellular fractions and those of ventricular fractions. The results showed that PGE2 was located mainly in the high-speed cytosolic supernatant fraction of the heart homogenates analyzed. Furthermore, PGE2 concentrations (ng/mg protein) were significantly higher in fractions obtained from atrial than those of ventricular tissue.
MeSH term(s)	Animals ; Dinoprostone/metabolism ; Female ; In Vitro Techniques ; Myocardium/metabolism ; Rats ; Rats, Inbred Strains ; Subcellular Fractions/metabolism ; Tissue Distribution
Chemical Substances	Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	1991-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639068-7
ISSN	1573-7241 ; 0920-3206
ISSN (online)	1573-7241
ISSN	0920-3206
DOI	10.1007/bf03029735
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Article: Oxytocic action of rat hypothalamus extracts.

CROXATTO, H / ZAMORANO, B

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)

2003 Volume 90, Issue 2, Page(s) 468–470

MeSH term(s)	Animals ; Female ; Humans ; Hypothalamus ; Labor, Induced ; Labor, Obstetric ; Oxytocics ; Pregnancy ; Rats
Chemical Substances	Oxytocics
Language	English
Publishing date	2003-09-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	4015-0
ISSN	1535-3699 ; 1525-1373 ; 0037-9727
ISSN (online)	1535-3699 ; 1525-1373
ISSN	0037-9727
DOI	10.3181/00379727-90-22068
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Article ; Online: Organellar Genomes from a ∼5,000-Year-Old Archaeological Maize Sample Are Closely Related to NB Genotype.

Pérez-Zamorano, Bernardo / Vallebueno-Estrada, Miguel / Martínez González, Javier / García Cook, Angel / Montiel, Rafael / Vielle-Calzada, Jean-Philippe / Delaye, Luis

Genome biology and evolution

2017 Volume 9, Issue 4, Page(s) 904–915

Abstract: The story of how preColumbian civilizations developed goes hand-in-hand with the process of plant domestication by Mesoamerican inhabitants. Here, we present the almost complete sequence of a mitochondrial genome and a partial chloroplast genome from an ... ...

Abstract	The story of how preColumbian civilizations developed goes hand-in-hand with the process of plant domestication by Mesoamerican inhabitants. Here, we present the almost complete sequence of a mitochondrial genome and a partial chloroplast genome from an archaeological maize sample collected at the Valley of Tehuacán, México. Accelerator mass spectrometry dated the maize sample to be 5,040-5,300 years before present (95% probability). Phylogenetic analysis of the mitochondrial genome shows that the archaeological sample branches basal to the other Zea mays genomes, as expected. However, this analysis also indicates that fertile genotype NB is closely related to the archaeological maize sample and evolved before cytoplasmic male sterility genotypes (CMS-S, CMS-T, and CMS-C), thus contradicting previous phylogenetic analysis of mitochondrial genomes from maize. We show that maximum-likelihood infers a tree where CMS genotypes branch at the base of the tree when including sites that have a relative fast rate of evolution thus suggesting long-branch attraction. We also show that Bayesian analysis infer a topology where NB and the archaeological maize sample are at the base of the tree even when including faster sites. We therefore suggest that previous trees suffered from long-branch attraction. We also show that the phylogenetic analysis of the ancient chloroplast is congruent with genotype NB to be more closely related to the archaeological maize sample. As shown here, the inclusion of ancient genomes on phylogenetic trees greatly improves our understanding of the domestication process of maize, one of the most important crops worldwide.
MeSH term(s)	Bayes Theorem ; Chloroplasts/genetics ; Evolution, Molecular ; Genome, Mitochondrial/genetics ; Genome, Plant ; Genotype ; Phylogeny ; Zea mays/genetics
Language	English
Publishing date	2017-04-01
Publishing country	England
Document type	Journal Article
ISSN	1759-6653
ISSN (online)	1759-6653
DOI	10.1093/gbe/evx048
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Article ; Online: Identification of cis-regulatory sequences reveals potential participation of lola and Deaf1 transcription factors in Anopheles gambiae innate immune response.

Pérez-Zamorano, Bernardo / Rosas-Madrigal, Sandra / Lozano, Oscar Arturo Migueles / Castillo Méndez, Manuel / Valverde-Garduño, Verónica

PloS one

2017 Volume 12, Issue 10, Page(s) e0186435

Abstract: The innate immune response of Anopheles gambiae involves the transcriptional upregulation of effector genes. Therefore, the cis-regulatory sequences and their cognate binding factors play essential roles in the mosquito's immune response. However, the ... ...

Abstract	The innate immune response of Anopheles gambiae involves the transcriptional upregulation of effector genes. Therefore, the cis-regulatory sequences and their cognate binding factors play essential roles in the mosquito's immune response. However, the genetic control of the mosquito's innate immune response is not yet fully understood. To gain further insight on the elements, the factors and the potential mechanisms involved, an open chromatin profiling was carried out on A. gambiae-derived immune-responsive cells. Here, we report the identification of cis-regulatory sites, immunity-related transcription factor binding sites, and cis-regulatory modules. A de novo motif discovery carried out on this set of cis-regulatory sequences identified immunity-related motifs and cis-regulatory modules. These modules contain motifs that are similar to binding sites for REL-, STAT-, lola- and Deaf1-type transcription factors. Sequence motifs similar to the binding sites for GAGA were found within a cis-regulatory module, together with immunity-related transcription factor binding sites. The presence of Deaf1- and lola-type binding sites, along with REL- and STAT-type binding sites, suggests that the immunity function of these two factors could have been conserved both in Drosophila and Anopheles gambiae.
MeSH term(s)	Animals ; Anopheles/genetics ; Anopheles/immunology ; Anopheles/metabolism ; Chromatin/genetics ; Genomics ; Immunity, Innate/genetics ; Insect Proteins/metabolism ; Regulatory Sequences, Nucleic Acid/genetics ; Transcription Factors/metabolism
Chemical Substances	Chromatin ; Insect Proteins ; Transcription Factors
Language	English
Publishing date	2017
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0186435
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Article: Prostaglandin-E2 and cyclic adenosine 3'-5' monophosphate levels in the hypertrophied rat heart.

Zamorano, B / Carmona, M T

Biological research

1992 Volume 25, Issue 2, Page(s) 85–89

Abstract: To assess whether prostaglandin-E2 (PGE2) and cyclic adenosine 3'-5'-monophosphate (cAMP) are involved in the cardiac response to chronic pressure overload, we measured by specific radioimmunoassay method the cardiac tissue and plasma concentrations of ... ...

Abstract	To assess whether prostaglandin-E2 (PGE2) and cyclic adenosine 3'-5'-monophosphate (cAMP) are involved in the cardiac response to chronic pressure overload, we measured by specific radioimmunoassay method the cardiac tissue and plasma concentrations of PGE2 and cAMP in an animal model of left ventricular hypertrophy. The cardiac hypertrophy was accompanied by a significant increase in PGE2 content, and a significant decrease in cAMP content, in the heart. In addition, we found elevated PGE2 and cAMP levels in arterial plasma samples from the rats with hypertrophied hearts compared to normal rats. These findings suggest a link between cardiac and vascular PGE2 and cAMP generation and the hemodynamic stresses of advanced cardiac overload.
MeSH term(s)	Animals ; Blood/metabolism ; Cardiomegaly/metabolism ; Cyclic AMP/metabolism ; Dinoprostone/metabolism ; Female ; Myocardium/metabolism ; Osmolar Concentration ; Radioimmunoassay ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Cyclic AMP (E0399OZS9N) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	1992
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1138990-4
ISSN	0716-9760
ISSN	0716-9760
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Article: Rate of displacement of oxytocic substances from dyencephalon to tuber cinereum in hypophysectomized rats.

SILVA MORENO, V / ZAMORANO, B / CROXATTO, H / BECERRA, M

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)

2003 Volume 92, Issue 2, Page(s) 352–353

MeSH term(s)	Animals ; Hypothalamus/physiology ; Oxytocics ; Pituitary Gland/surgery ; Rats ; Tuber Cinereum
Chemical Substances	Oxytocics
Language	English
Publishing date	2003-09-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	4015-0
ISSN	1535-3699 ; 1525-1373 ; 0037-9727
ISSN (online)	1535-3699 ; 1525-1373
ISSN	0037-9727
DOI	10.3181/00379727-92-22474
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Article: Subcellular distribution of prostaglandin-E2 and prostaglandin-F2 alpha in atrial tissue from patients with mitral valve disease.

Zamorano, B / Escobar, E / Gazmuri, R

Biological research

1998 Volume 31, Issue 4, Page(s) 343–349

Abstract: The distribution of prostaglandin-E2 (PGE2) and prostaglandin-F2 alpha (PGF2 alpha) was studied in subcellular fractions isolated from homogenates of human atrial fresh tissue by differential centrifugation. Right and left atrial samples were excised ... ...

Abstract	The distribution of prostaglandin-E2 (PGE2) and prostaglandin-F2 alpha (PGF2 alpha) was studied in subcellular fractions isolated from homogenates of human atrial fresh tissue by differential centrifugation. Right and left atrial samples were excised from the same heart of six patients with mitral valve disease at the time of open heart surgery. The atrial fractions investigated were mitochondrial (8,500 g pellet), microsomal (100,000 g pellet) and cytosol soluble (100,000 g supernatant) fractions. After extraction of prostaglandins from the three atrial fractions and separation of PGE from PGF series by chromatography on silicic acid column, these prostaglandins were measured by radioimmunoassay. The results showed that PGE2 and PGF2 alpha were located mainly in the soluble cytosolic fraction of right and left atrial tissue (p < 0.001). Furthermore, the prostaglandins levels were higher in left than in right atria of these patients (p < 0.001). The relation between prostaglandins heart generation in response to elevated work load of mitral valve disease is discussed.
MeSH term(s)	Adult ; Dinoprost/analysis ; Dinoprost/metabolism ; Dinoprostone/analysis ; Dinoprostone/metabolism ; Female ; Heart Atria/chemistry ; Heart Atria/metabolism ; Humans ; Male ; Mitral Valve/chemistry ; Mitral Valve Insufficiency/metabolism ; Mitral Valve Stenosis/metabolism ; Oxytocics/analysis ; Oxytocics/metabolism ; Subcellular Fractions
Chemical Substances	Oxytocics ; Dinoprost (B7IN85G1HY) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	1998
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1138990-4
ISSN	0716-9760
ISSN	0716-9760
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Article: Vascular smooth muscle reactivity to norepinephrine in ovariectomized rats: relationship to vascular PGE2/PGF2 alpha ratio.

Zamorano, B / Bruzzone, M E / Martinez, J L

General pharmacology

1995 Volume 26, Issue 7, Page(s) 1613–1618

Abstract: 1. We have recently demonstrated that, in normal female rats, vascular reactivity (VR) and vascular prostaglandin-E2 (PGE2) and prostacyclin production are influenced by the ovarian cycle. 2. In this study, we investigated the vascular reactivity (VR) of ...

Abstract	1. We have recently demonstrated that, in normal female rats, vascular reactivity (VR) and vascular prostaglandin-E2 (PGE2) and prostacyclin production are influenced by the ovarian cycle. 2. In this study, we investigated the vascular reactivity (VR) of isolated rat thoracic aorta to norepinephrine (NE 10(-12)-10(-6)M) in ovariectomized rats (OVX-rats), 48 hr and 8 days after surgical removal of the ovaries and in normally cycling rats (NR) at the proestrus stage of the estrous cycle, when the level of circulating estrogen was higher. 3. In addition, we determined the vascular synthesis of PGE2 and protaglandin-F2 alpha (PGF2 alpha) in both groups of OVX-rats, and in NR during the proestrous stage. 4. The results showed that VR to NE 10(-12)-10(-10) M was similar between OVX-rats and normal rats. 5. However, aortic rings obtained from both groups of OVX-rats showed a significant increase of the contraction response induced by NE 10(-9)-10(-6) M. 6. Furthermore, the contractile response to NE 10(-7)-10(-6) M was greater in the aortic rings from OVX-rats 8 days after ovariectomy compared to OVX-rats 48 hr (p < 0.001). 7. Vascular PGE2 and PGF2 alpha synthesis (ng/mg protein/hr) was significantly higher in both groups of OVX-rats than NR. But the vascular PGF2 alpha synthesis increased more than PGE2 in these OVX-rats, thus the ratio PGE2/PGF2 alpha was decreased significantly in both groups of OVX-rats (p < 0.01 and p < 0.001 OVX-rats 48 hr and 8 days after surgery, respectively) compared to NR-ratio.
MeSH term(s)	Adrenergic alpha-Agonists/pharmacology ; Animals ; Aorta, Thoracic ; Dinoprost/metabolism ; Dinoprostone/metabolism ; Dose-Response Relationship, Drug ; Female ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/physiology ; Norepinephrine/pharmacology ; Ovariectomy ; Oxytocics/metabolism ; Pregnancy ; Proestrus ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Adrenergic alpha-Agonists ; Oxytocics ; Dinoprost (B7IN85G1HY) ; Dinoprostone (K7Q1JQR04M) ; Norepinephrine (X4W3ENH1CV)
Language	English
Publishing date	1995-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	190071-7
ISSN	1879-0011 ; 0306-3623
ISSN (online)	1879-0011
ISSN	0306-3623
DOI	10.1016/0306-3623(95)00060-7
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