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  1. AU="Zaniar Ghazizadeh"
  2. AU="Rathod, Aniruddha"
  3. AU=Ong Edison
  4. AU="Hoffmann, Daniela"
  5. AU="Mallett, Garry"
  6. AU=Lemos Pedro A
  7. AU="Bakris, George L."
  8. AU="Tun-Linn Thein"
  9. AU="Michelle Schinkel"
  10. AU="Scolieri, G"

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  1. Artikel ; Online: A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells

    Zaniar Ghazizadeh / Jiajun Zhu / Faranak Fattahi / Alice Tang / Xiaolu Sun / Sadaf Amin / Su-Yi Tsai / Mona Khalaj / Ting Zhou / Ryan M. Samuel / Tuo Zhang / Francis A. Ortega / Miriam Gordillo / Dorota Moroziewicz / Daniel Paull / Scott A. Noggle / Jenny Zhaoying Xiang / Lorenz Studer / David J. Christini /
    Geoffrey S. Pitt / Todd Evans / Shuibing Chen

    iScience, Vol 25, Iss 4, Pp 104153- (2022)

    2022  

    Abstract: Summary: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods. We developed a dual SHOX2:GFP; MYH6:mCherry knockin ... ...

    Abstract Summary: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods. We developed a dual SHOX2:GFP; MYH6:mCherry knockin human embryonic stem cell (hESC) reporter line, which allows the identification and purification of SAN-like cells. Using this line, we performed several rounds of chemical screens and developed an efficient strategy to generate and purify hESC-derived SAN-like cells (hESC-SAN). The derived hESC-SAN cells display molecular and electrophysiological characteristics of bona fide nodal cells, which allowed exploration of their transcriptional profile at single-cell level. In sum, our dual reporter system facilitated an effective strategy for deriving human SAN-like cells, which can potentially be used for future disease modeling and drug discovery.
    Schlagwörter Biological sciences ; Stem cells research ; Methodology in biological sciences ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2022-04-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: ROCKII inhibition promotes the maturation of human pancreatic beta-like cells

    Zaniar Ghazizadeh / Der-I Kao / Sadaf Amin / Brandoch Cook / Sahana Rao / Ting Zhou / Tuo Zhang / Zhaoying Xiang / Reyn Kenyon / Omer Kaymakcalan / Chengyang Liu / Todd Evans / Shuibing Chen

    Nature Communications, Vol 8, Iss 1, Pp 1-

    2017  Band 12

    Abstract: Our incomplete understanding of how pancreatic beta cells form limits the generation of beta-like cells from human pluripotent stem cells (hPSC). Here, the authors identify a ROCKII inhibitor H1152 as increasing insulin secreting cells from hPSCs and ... ...

    Abstract Our incomplete understanding of how pancreatic beta cells form limits the generation of beta-like cells from human pluripotent stem cells (hPSC). Here, the authors identify a ROCKII inhibitor H1152 as increasing insulin secreting cells from hPSCs and improving beta-cell maturation on transplantation in vivo.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2017-08-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Discovery of a drug candidate for GLIS3-associated diabetes

    Sadaf Amin / Brandoch Cook / Ting Zhou / Zaniar Ghazizadeh / Raphael Lis / Tuo Zhang / Mona Khalaj / Miguel Crespo / Manuradhi Perera / Jenny Zhaoying Xiang / Zengrong Zhu / Mark Tomishima / Chengyang Liu / Ali Naji / Todd Evans / Danwei Huangfu / Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 12

    Abstract: GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in ... ...

    Abstract GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in GLIS3 mutants by inhibiting the abnormally activated TGFβ pathway.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-07-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Discovery of a drug candidate for GLIS3-associated diabetes

    Sadaf Amin / Brandoch Cook / Ting Zhou / Zaniar Ghazizadeh / Raphael Lis / Tuo Zhang / Mona Khalaj / Miguel Crespo / Manuradhi Perera / Jenny Zhaoying Xiang / Zengrong Zhu / Mark Tomishima / Chengyang Liu / Ali Naji / Todd Evans / Danwei Huangfu / Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 12

    Abstract: GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in ... ...

    Abstract GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in GLIS3 mutants by inhibiting the abnormally activated TGFβ pathway.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-07-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Prospective Isolation of ISL1+ Cardiac Progenitors from Human ESCs for Myocardial Infarction Therapy

    Zaniar Ghazizadeh / Faranak Fattahi / Mehdi Mirzaei / Delger Bayersaikhan / Jaesuk Lee / Sehyun Chae / Daehee Hwang / Kyunghee Byun / Mehdi Sharifi Tabar / Sara Taleahmad / Shahab Mirshahvaladi / Parisa Shabani / Hananeh Fonoudi / Paul A. Haynes / Hossein Baharvand / Nasser Aghdami / Todd Evans / Bonghee Lee / Ghasem Hosseini Salekdeh

    Stem Cell Reports, Vol 10, Iss 3, Pp 848-

    2018  Band 859

    Abstract: Summary: The LIM-homeodomain transcription factor ISL1 marks multipotent cardiac progenitors that give rise to cardiac muscle, endothelium, and smooth muscle cells. ISL1+ progenitors can be derived from human pluripotent stem cells, but the inability to ... ...

    Abstract Summary: The LIM-homeodomain transcription factor ISL1 marks multipotent cardiac progenitors that give rise to cardiac muscle, endothelium, and smooth muscle cells. ISL1+ progenitors can be derived from human pluripotent stem cells, but the inability to efficiently isolate pure populations has limited their characterization. Using a genetic selection strategy, we were able to highly enrich ISL1+ cells derived from human embryonic stem cells. Comparative quantitative proteomic analysis of enriched ISL1+ cells identified ALCAM (CD166) as a surface marker that enabled the isolation of ISL1+ progenitor cells. ALCAM+/ISL1+ progenitors are multipotent and differentiate into cardiomyocytes, endothelial cells, and smooth muscle cells. Transplantation of ALCAM+ progenitors enhances tissue recovery, restores cardiac function, and improves angiogenesis through activation of AKT-MAPK signaling in a rat model of myocardial infarction, based on cardiac MRI and histology. Our study establishes an efficient method for scalable purification of human ISL1+ cardiac precursor cells for therapeutic applications. : In this article, Salekdeh and colleagues show that ISL1+ cardiac progenitors can be purified from a heterogeneous population of hESC-derived cardiomyocytes using ALCAM. Transplantation of multipotent ISL1+/ALCAM+ progenitors enhances tissue recovery, restores cardiac function, and improves angiogenesis in a rat model of myocardial infarction, based on cardiac MRI and histology. Keywords: cell therapy, proteomics, myocardial biology, stem cells
    Schlagwörter Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2018-03-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival

    Ting Zhou / Tae Wan Kim / Chi Nok Chong / Lei Tan / Sadaf Amin / Zohreh Sadat Badieyan / Suranjit Mukherjee / Zaniar Ghazizadeh / Hui Zeng / Min Guo / Miguel Crespo / Tuo Zhang / Reyn Kenyon / Christopher L. Robinson / Effie Apostolou / Hui Wang / Jenny Zhaoying Xiang / Todd Evans / Lorenz Studer /
    Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 13

    Abstract: Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that ... ...

    Abstract Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that loss of the gene GSTT1 confers hypersensitivity.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-11-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival

    Ting Zhou / Tae Wan Kim / Chi Nok Chong / Lei Tan / Sadaf Amin / Zohreh Sadat Badieyan / Suranjit Mukherjee / Zaniar Ghazizadeh / Hui Zeng / Min Guo / Miguel Crespo / Tuo Zhang / Reyn Kenyon / Christopher L. Robinson / Effie Apostolou / Hui Wang / Jenny Zhaoying Xiang / Todd Evans / Lorenz Studer /
    Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 13

    Abstract: Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that ... ...

    Abstract Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that loss of the gene GSTT1 confers hypersensitivity.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-11-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells.

    Hananeh Fonoudi / Meghdad Yeganeh / Faranak Fattahi / Zaniar Ghazizadeh / Hassan Rassouli / Mehdi Alikhani / Bahareh Adhami Mojarad / Hossein Baharvand / Ghasem Hosseini Salekdeh / Nasser Aghdami

    PLoS ONE, Vol 8, Iss 1, p e

    2013  Band 55577

    Abstract: BACKGROUND: Human embryonic stem cells (hESCs) have the potential to provide an unlimited source of cardiomyocytes, which are invaluable resources for drug or toxicology screening, medical research, and cell therapy. Currently a number of obstacles exist ...

    Abstract BACKGROUND: Human embryonic stem cells (hESCs) have the potential to provide an unlimited source of cardiomyocytes, which are invaluable resources for drug or toxicology screening, medical research, and cell therapy. Currently a number of obstacles exist such as the insufficient efficiency of differentiation protocols, which should be overcome before hESC-derived cardiomyocytes can be used for clinical applications. Although the differentiation efficiency can be improved by the genetic manipulation of hESCs to over-express cardiac-specific transcription factors, these differentiated cells are not safe enough to be applied in cell therapy. Protein transduction has been demonstrated as an alternative approach for increasing the efficiency of hESCs differentiation toward cardiomyocytes. METHODS: We present an efficient protocol for the differentiation of hESCs in suspension by direct introduction of a LIM homeodomain transcription factor, Islet1 (ISL1) recombinant protein into the cells. RESULTS: We found that the highest beating clusters were derived by continuous treatment of hESCs with 40 µg/ml recombinant ISL1 protein during days 1-8 after the initiation of differentiation. The treatment resulted in up to a 3-fold increase in the number of beating areas. In addition, the number of cells that expressed cardiac specific markers (cTnT, CONNEXIN 43, ACTININ, and GATA4) doubled. This protocol was also reproducible for another hESC line. CONCLUSIONS: This study has presented a new, efficient, and reproducible procedure for cardiomyocytes differentiation. Our results will pave the way for scaled up and controlled differentiation of hESCs to be used for biomedical applications in a bioreactor culture system.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 571
    Sprache Englisch
    Erscheinungsdatum 2013-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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