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  1. Article ; Online: Evaluation of commercial qPCR kits for detection of SARS-CoV-2 in pooled samples

    Petrovan, Vlad / Vrajmasu, Virgil / Dimon, Paula / Zaulet, Mihaela

    bioRxiv

    Abstract: Due to the current pandemic, global shortage of reagents has drawn interest in developing alternatives to increase the number coronavirus tests. One such alternative is sample pooling. Here we compared commercial kits that are used in COVID-19 ... ...

    Abstract Due to the current pandemic, global shortage of reagents has drawn interest in developing alternatives to increase the number coronavirus tests. One such alternative is sample pooling. Here we compared commercial kits that are used in COVID-19 diagnostics, in terms of sensitivity and feasibility for use in pooling. We showed that pooling of up to 60 samples did not affect the efficiency of the kits. Also, the RNA dependent RNA polymerase (RdRp) is a more suitable target in pooled samples than the Envelope (E) protein. This approach could provide an easy method of screening large number of samples and help adjust different government regulations.
    Keywords covid19
    Language English
    Publishing date 2020-05-28
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2020.05.28.120667
    Database COVID19

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  2. Article ; Online: Evaluation of commercial qPCR kits for detection of SARS-CoV-2 in pooled samples

    Petrovan, Vlad / Vrajmasu, Virgil / Dimon, Paula / Zaulet, Mihaela

    bioRxiv

    Abstract: Due to the current pandemic, global shortage of reagents has drawn interest in developing alternatives to increase the number coronavirus tests. One such alternative is sample pooling. Here we compared commercial kits that are used in COVID-19 ... ...

    Abstract Due to the current pandemic, global shortage of reagents has drawn interest in developing alternatives to increase the number coronavirus tests. One such alternative is sample pooling. Here we compared commercial kits that are used in COVID-19 diagnostics, in terms of sensitivity and feasibility for use in pooling. We showed that pooling of up to 60 samples did not affect the efficiency of the kits. Also, the RNA dependent RNA polymerase (RdRp) is a more suitable target in pooled samples than the Envelope (E) protein. This approach could provide an easy method of screening large number of samples and help adjust different government regulations.
    Keywords covid19
    Publisher BioRxiv; WHO
    Document type Article ; Online
    DOI 10.1101/2020.05.28.120667
    Database COVID19

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Bacterial Cellulose Hybrid Composites with Calcium Phosphate for Bone Tissue Regeneration.

    Busuioc, Cristina / Isopencu, Gabriela / Banciu, Adela / Banciu, Daniel-Dumitru / Oprea, Ovidiu / Mocanu, Alexandra / Deleanu, Iuliana / Zăuleţ, Mihaela / Popescu, Laura / Tănăsuică, Rodica / Vasilescu, Mihai / Stoica-Guzun, Anicuţa

    International journal of molecular sciences

    2022  Volume 23, Issue 24

    Abstract: Bacterial cellulose (BC) is a unique microbial biopolymer with a huge number of significant applications in the biomedical field, including bone tissue engineering. The present study proposes to obtain and characterize BC hybrid composites with calcium ... ...

    Abstract Bacterial cellulose (BC) is a unique microbial biopolymer with a huge number of significant applications in the biomedical field, including bone tissue engineering. The present study proposes to obtain and characterize BC hybrid composites with calcium phosphate as biocompatible and bioactive membranes for bone tissue engineering. BC precursor membranes were obtained in static culture fermentation, and after purification, were oxidized to obtain 2,3-dialdehyde bacterial cellulose (DABC). Calcium phosphate-BC oxidized membranes were produced by successive immersion in precursor solutions under ultrasonic irradiation. The samples were characterized for their physicochemical properties using scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy grazing incidence X-ray diffraction (GI-XRD), solid-state
    Language English
    Publishing date 2022-12-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232416180
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples.

    Petrovan, Vlad / Vrajmasu, Virgil / Bucur, Ana Cristina / Soare, Dan Sebastian / Radu, Eugen / Dimon, Paula / Zaulet, Mihaela

    Diagnostics (Basel, Switzerland)

    2020  Volume 10, Issue 7

    Abstract: Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 ... ...

    Abstract Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 diagnostics in terms of their sensitivity and feasibility for use in pooling. In this preliminary study, we showed that pooling of up to 80 samples did not affect the efficacy of the kits. Additionally, the RNA-dependent RNA polymerase (RdRp) gene is a more suitable target in pooled samples than the envelope (E) gene. This approach could provide an easy method of screening a large number of samples and help adjust different governmental regulations.
    Keywords covid19
    Language English
    Publishing date 2020-07-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics10070472
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Identification and prevalence of swine pasivirus 1 in eastern Romanian pig farms.

    Zaulet, Mihaela / Petrovan, Vlad / Birladeanu, Andrada M / Stoian, Ana Maria M / Kevorkian, Steliana E M / Nichita, Cornelia / Eloit, Marc / Buburuzan, Laura

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

    2017  Volume 29, Issue 3, Page(s) 305–311

    Abstract: Swine pasivirus 1 (SPaV-1) was first detected in the feces of healthy pigs in France as a new species in family Picornaviridae. We investigated the presence, distribution, and genetic variability of this virus in 7 geographic areas with intensive pig ... ...

    Abstract Swine pasivirus 1 (SPaV-1) was first detected in the feces of healthy pigs in France as a new species in family Picornaviridae. We investigated the presence, distribution, and genetic variability of this virus in 7 geographic areas with intensive pig breeding farms in eastern Romania. A total of 564 porcine specimens, including 82 fecal specimens and 482 pools of organs, were collected from healthy pigs in different stages of production from pathogen-free swine farming units. The virus was found in 6 of 7 areas investigated. Of the 564 samples analyzed, 218 were positive for SPaV-1, with the highest prevalence of the virus in organ homogenates (39% positive) followed by feces (37% positive). The highest susceptibility to infection was found in nurseries (50% positive in both the first and second months of feeding). Sequencing analysis of VP0 revealed 3 different Romanian sequences. The phylogenetic investigations suggest that the Romanian sequences cluster with other Pasivirus strains selected from the GenBank database, forming a separate clade from other Picornaviridae genera and defining the described Pasivirus.
    MeSH term(s) Animals ; Farms ; Feces/virology ; Phylogeny ; Picornaviridae/classification ; Picornaviridae/genetics ; Picornaviridae/isolation & purification ; Picornaviridae Infections/epidemiology ; Picornaviridae Infections/veterinary ; Picornaviridae Infections/virology ; Prevalence ; Romania/epidemiology ; Specific Pathogen-Free Organisms ; Swine ; Swine Diseases/epidemiology ; Swine Diseases/etiology ; Swine Diseases/virology
    Language English
    Publishing date 2017-03-31
    Publishing country United States
    Document type Journal Article ; Multicenter Study
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/1040638717696044
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3645: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.

    Zaulet, Mihaela / Gurau, Maria Rodica / Petrovan, Vlad / Buburuzan, Laura

    International journal of molecular sciences

    2012  Volume 13, Issue 9, Page(s) 12046–12061

    Abstract: Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as ... ...

    Abstract Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.
    MeSH term(s) Animals ; Genetic Variation ; Open Reading Frames ; Phylogeny ; Porcine respiratory and reproductive syndrome virus/classification ; Porcine respiratory and reproductive syndrome virus/genetics ; Porcine respiratory and reproductive syndrome virus/isolation & purification ; Romania ; Swine
    Language English
    Publishing date 2012-09-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms130912046
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Identification and prevalence of swine pasivirus 1 in eastern Romanian pig farms

    Zaulet, Mihaela / Vlad Petrovan / Andrada M. Birladeanu / Ana Maria M. Stoian / Steliana E. M. Kevorkian / Cornelia Nichita / Marc Eloit / Laura Buburuzan

    Journal of veterinary diagnostic investigation. , v. 29, no. 3

    2017  

    Abstract: Swine pasivirus 1 (SPaV-1) was first detected in the feces of healthy pigs in France as a new species in family Picornaviridae. We investigated the presence, distribution, and genetic variability of this virus in 7 geographic areas with intensive pig ... ...

    Abstract Swine pasivirus 1 (SPaV-1) was first detected in the feces of healthy pigs in France as a new species in family Picornaviridae. We investigated the presence, distribution, and genetic variability of this virus in 7 geographic areas with intensive pig breeding farms in eastern Romania. A total of 564 porcine specimens, including 82 fecal specimens and 482 pools of organs, were collected from healthy pigs in different stages of production from pathogen-free swine farming units. The virus was found in 6 of 7 areas investigated. Of the 564 samples analyzed, 218 were positive for SPaV-1, with the highest prevalence of the virus in organ homogenates (39% positive) followed by feces (37% positive). The highest susceptibility to infection was found in nurseries (50% positive in both the first and second months of feeding). Sequencing analysis of VP0 revealed 3 different Romanian sequences. The phylogenetic investigations suggest that the Romanian sequences cluster with other Pasivirus strains selected from the GenBank database, forming a separate clade from other Picornaviridae genera and defining the described Pasivirus.
    Keywords Picornaviridae ; farms ; feces ; genetic databases ; genetic variation ; livestock and meat industry ; livestock production ; new species ; phylogeny ; pig breeding ; sequence analysis ; swine ; viruses ; France ; Romania
    Language English
    Dates of publication 2017-05
    Size p. 305-311.
    Publishing place SAGE Publications
    Document type Article
    ZDB-ID 287603-6
    ISSN 1943-4936 ; 1040-6387
    ISSN (online) 1943-4936
    ISSN 1040-6387
    DOI 10.1177/1040638717696044
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3645: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: Protective effects of silymarin against bisphenol A-induced hepatotoxicity in mouse liver.

    Zaulet, Mihaela / Kevorkian, Steliana Elvira Maria / Dinescu, Sorina / Cotoraci, Coralia / Suciu, Maria / Herman, Hildegard / Buburuzan, Laura / Badulescu, Liliana / Ardelean, Aurel / Hermenean, Anca

    Experimental and therapeutic medicine

    2017  Volume 13, Issue 3, Page(s) 821–828

    Abstract: Bisphenol A (BPA) is an endocrine-disrupting chemical released into the environment, with severe consequences for human health, including metabolic syndrome and associated pathological conditions. Due to limited information on BPA-induced hepatotoxicity, ...

    Abstract Bisphenol A (BPA) is an endocrine-disrupting chemical released into the environment, with severe consequences for human health, including metabolic syndrome and associated pathological conditions. Due to limited information on BPA-induced hepatotoxicity, the present study focused on investigating the association between BPA-induced toxicity and inflammatory markers in the liver, and how these injuries may be alleviated using the natural agent silymarin, a flavonoid with antioxidant properties obtained from
    Language English
    Publishing date 2017-01-20
    Publishing country Greece
    Document type Journal Article
    ZDB-ID 2683844-8
    ISSN 1792-1015 ; 1792-0981
    ISSN (online) 1792-1015
    ISSN 1792-0981
    DOI 10.3892/etm.2017.4066
    Database MEDical Literature Analysis and Retrieval System OnLINE

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