LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 42

Search options

  1. Article: Editorial: Probiotic bacteria-derived effector molecules and their impact on the host in health and disease.

    Górska, Sabina / Schwarzer, Martin / Schabussova, Irma / Zawilak-Pawlik, Anna Magdalena / Sandström, Corine

    Frontiers in microbiology

    2022  Volume 13, Page(s) 1089461

    Language English
    Publishing date 2022-11-29
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.1089461
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach.

    Noszka, Mateusz / Strzałka, Agnieszka / Muraszko, Jakub / Kolenda, Rafał / Meng, Chen / Ludwig, Christina / Stingl, Kerstin / Zawilak-Pawlik, Anna

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 6715

    Abstract: The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, ... ...

    Abstract The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicate that HP1021 modulates H. pylori's response to oxidative stress. HP1021 controls the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins are differently expressed in the HP1021 deletion mutant. HP1021 controls typical ROS response pathways (katA, rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. HP1021 is a molecular regulator of competence in H. pylori, as HP1021-dependent repression of the comB DNA uptake genes is relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controls glucose consumption by directly regulating the gluP transporter and has an important impact on maintaining the energetic balance in the cell.
    MeSH term(s) Humans ; Helicobacter pylori/genetics ; Helicobacter pylori/metabolism ; Regulon/genetics ; Reactive Oxygen Species/metabolism ; Proteomics ; Multiomics ; Oxidation-Reduction ; DNA/metabolism ; Bacterial Proteins/metabolism ; Helicobacter Infections/genetics
    Chemical Substances Reactive Oxygen Species ; DNA (9007-49-2) ; Bacterial Proteins
    Language English
    Publishing date 2023-10-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-42364-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: The Role of the N-Terminal Domains of Bacterial Initiator DnaA in the Assembly and Regulation of the Bacterial Replication Initiation Complex.

    Zawilak-Pawlik, Anna / Nowaczyk, Małgorzata / Zakrzewska-Czerwińska, Jolanta

    Genes

    2017  Volume 8, Issue 5

    Abstract: The primary role of the bacterial protein DnaA is to initiate chromosomal replication. The DnaA protein binds to DNA at the origin of chromosomal replication ( ...

    Abstract The primary role of the bacterial protein DnaA is to initiate chromosomal replication. The DnaA protein binds to DNA at the origin of chromosomal replication (
    Language English
    Publishing date 2017-05-10
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes8050136
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Recent Advances in Helicobacter pylori Replication: Possible Implications in Adaptation to a Pathogenic Lifestyle and Perspectives for Drug Design.

    Zawilak-Pawlik, Anna / Zakrzewska-Czerwińska, Jolanta

    Current topics in microbiology and immunology

    2017  Volume 400, Page(s) 73–103

    Abstract: DNA replication is an important step in the life cycle of every cell that ensures the continuous flow of genetic information from one generation to the next. In all organisms, chromosome replication must be coordinated with overall cell growth. ... ...

    Abstract DNA replication is an important step in the life cycle of every cell that ensures the continuous flow of genetic information from one generation to the next. In all organisms, chromosome replication must be coordinated with overall cell growth. Helicobacter pylori growth strongly depends on its interaction with the host, particularly with the gastric epithelium. Moreover, H. pylori actively searches for an optimal microniche within a stomach, and it has been shown that not every microniche equally supports growth of this bacterium. We postulate that besides nutrients, H. pylori senses different, unknown signals, which presumably also affect chromosome replication to maintain H. pylori propagation at optimal ratio allowing H. pylori to establish a chronic, lifelong infection. Thus, H. pylori chromosome replication and particularly the regulation of this process might be considered important for bacterial pathogenesis. Here, we summarize our current knowledge of chromosome and plasmid replication in H. pylori and discuss the mechanisms responsible for regulating this key cellular process. The results of extensive studies conducted thus far allow us to propose common and unique traits in H. pylori chromosome replication. Interestingly, the repertoire of proteins involved in replication in H. pylori is significantly different to that in E. coli, strongly suggesting that novel factors are engaged in H. pylori chromosome replication and could represent attractive drug targets.
    MeSH term(s) Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Chromosomes, Bacterial/genetics ; Chromosomes, Bacterial/metabolism ; DNA Replication/drug effects ; DNA, Bacterial/genetics ; DNA, Bacterial/metabolism ; Drug Design ; Helicobacter pylori/drug effects ; Helicobacter pylori/genetics ; Helicobacter pylori/physiology ; Humans
    Chemical Substances Anti-Bacterial Agents ; DNA, Bacterial
    Language English
    Publishing date 2017
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ISSN 0070-217X
    ISSN 0070-217X
    DOI 10.1007/978-3-319-50520-6_4
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Putative Cooperative ATP-DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon

    Jaworski, Pawel / Zyla-Uklejewicz, Dorota / Nowaczyk-Cieszewska, Malgorzata / Donczew, Rafal / Mielke, Thorsten / Weigel, Christoph / Zawilak-Pawlik, Anna

    International journal of molecular sciences

    2021  Volume 22, Issue 12

    Abstract: ... ...

    Abstract oriC
    MeSH term(s) Adenosine Triphosphate/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Helicobacter pylori/genetics ; Helicobacter pylori/metabolism ; Mutation ; Origin Recognition Complex
    Chemical Substances Bacterial Proteins ; DNA-Binding Proteins ; DnaA protein, Bacteria ; OriC chromosomal replication origin ; Origin Recognition Complex ; Adenosine Triphosphate (8L70Q75FXE)
    Language English
    Publishing date 2021-06-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22126643
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: HP1021 is a redox switch protein identified in Helicobacter pylori.

    Szczepanowski, Piotr / Noszka, Mateusz / Żyła-Uklejewicz, Dorota / Pikuła, Fabian / Nowaczyk-Cieszewska, Malgorzata / Krężel, Artur / Stingl, Kerstin / Zawilak-Pawlik, Anna

    Nucleic acids research

    2021  Volume 49, Issue 12, Page(s) 6863–6879

    Abstract: Helicobacter pylori is a gram-negative, microaerophilic, pathogenic bacterium and a widespread colonizer of humans. H. pylori has developed mechanisms that enable it to overcome the harsh environment of the human stomach, including reactive oxygen ... ...

    Abstract Helicobacter pylori is a gram-negative, microaerophilic, pathogenic bacterium and a widespread colonizer of humans. H. pylori has developed mechanisms that enable it to overcome the harsh environment of the human stomach, including reactive oxygen species (ROS). Interestingly, up to now no typical regulator dedicated to the oxidative-stress response has been discovered. In this work, we reveal that the inhibitor of replication initiation HP1021 functions as a redox switch protein in H. pylori and plays an important role in response to oxidative stress of the gastric pathogen. Each of the two predicted HP1021 domains contains three cysteine residues. We show that the cysteine residues of HP1021 are sensitive to oxidation both in vitro and in vivo, and we demonstrate that HP1021 DNA-binding activity to oriC depends on the redox state of the protein. Moreover, Zn2+ modulates HP1021 affinity towards oriC template DNA. Transcription analysis of selected H. pylori genes by RT-qPCR indicated that HP1021 is directly involved in the oxygen-dependent control of H. pylori fecA3 and gluP genes, which are implicated in response to oxidative stress. In conclusion, HP1021 is a redox switch protein and could be a target for H. pylori control strategies.
    MeSH term(s) Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Bacterial Proteins/physiology ; Cations, Divalent/metabolism ; DNA/metabolism ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/metabolism ; DNA-Binding Proteins/physiology ; Gene Expression Regulation, Bacterial ; Helicobacter pylori/genetics ; Helicobacter pylori/metabolism ; Models, Molecular ; Oxidation-Reduction ; Oxidative Stress ; Protein Binding ; Protein Domains ; Transcription, Genetic
    Chemical Substances Bacterial Proteins ; Cations, Divalent ; DNA-Binding Proteins ; DNA (9007-49-2)
    Language English
    Publishing date 2021-06-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkab440
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Chaperone activity of serine protease HtrA of Helicobacter pylori as a crucial survival factor under stress conditions.

    Zarzecka, Urszula / Harrer, Aileen / Zawilak-Pawlik, Anna / Skorko-Glonek, Joanna / Backert, Steffen

    Cell communication and signaling : CCS

    2019  Volume 17, Issue 1, Page(s) 161

    Abstract: Background: Serine protease HtrA exhibits both proteolytic and chaperone activities, which are involved in cellular protein quality control. Moreover, HtrA is an important virulence factor in many pathogens including Helicobacter pylori, for which the ... ...

    Abstract Background: Serine protease HtrA exhibits both proteolytic and chaperone activities, which are involved in cellular protein quality control. Moreover, HtrA is an important virulence factor in many pathogens including Helicobacter pylori, for which the crucial stage of infection is the cleavage of E-cadherin and other cell-to-cell junction proteins.
    Methods: The in vitro study of H. pylori HtrA (HtrA
    Results: We demonstrate that HtrA
    Conclusion: Here we identified the chaperone activity of the HtrA
    MeSH term(s) Helicobacter pylori/enzymology ; Helicobacter pylori/metabolism ; Humans ; Hydrogen-Ion Concentration ; Molecular Chaperones/metabolism ; Serine Proteases/metabolism ; Signal Transduction ; Stress, Physiological ; Tumor Cells, Cultured
    Chemical Substances Molecular Chaperones ; Serine Proteases (EC 3.4.-)
    Language English
    Publishing date 2019-12-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1478-811X
    ISSN (online) 1478-811X
    DOI 10.1186/s12964-019-0481-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Structural Insights into New Bi(III) Coordination Polymers with Pyridine-2,3-Dicarboxylic Acid: Photoluminescence Properties and Anti-

    Kowalik, Mateusz / Masternak, Joanna / Łakomska, Iwona / Kazimierczuk, Katarzyna / Zawilak-Pawlik, Anna / Szczepanowski, Piotr / Khavryuchenko, Oleksiy V / Barszcz, Barbara

    International journal of molecular sciences

    2020  Volume 21, Issue 22

    Abstract: Two novel coordination polymers, [ ... ...

    Abstract Two novel coordination polymers, [Bi
    MeSH term(s) Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Bismuth/chemistry ; Coordination Complexes/chemistry ; Coordination Complexes/pharmacology ; Dicarboxylic Acids/chemistry ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/pharmacology ; Helicobacter pylori/growth & development ; Pyridines/chemistry
    Chemical Substances Anti-Bacterial Agents ; Coordination Complexes ; Dicarboxylic Acids ; Fluorescent Dyes ; Pyridines ; Bismuth (U015TT5I8H)
    Language English
    Publishing date 2020-11-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms21228696
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article: Structure and Function of the

    Jaworski, Pawel / Donczew, Rafal / Mielke, Thorsten / Weigel, Christoph / Stingl, Kerstin / Zawilak-Pawlik, Anna

    Frontiers in microbiology

    2018  Volume 9, Page(s) 1533

    Abstract: Campylobacter ... ...

    Abstract Campylobacter jejuni
    Language English
    Publishing date 2018-07-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2018.01533
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Beyond DnaA: the role of DNA topology and DNA methylation in bacterial replication initiation.

    Donczew, Rafał / Zakrzewska-Czerwińska, Jolanta / Zawilak-Pawlik, Anna

    Journal of molecular biology

    2014  Volume 426, Issue 12, Page(s) 2269–2282

    Abstract: The replication of chromosomal DNA is a fundamental event in the life cycle of every cell. The first step of replication, initiation, is controlled by multiple factors to ensure only one round of replication per cell cycle. The process of initiation has ... ...

    Abstract The replication of chromosomal DNA is a fundamental event in the life cycle of every cell. The first step of replication, initiation, is controlled by multiple factors to ensure only one round of replication per cell cycle. The process of initiation has been described most thoroughly for bacteria, especially Escherichia coli, and involves many regulatory proteins that vary considerably between different species. These proteins control the activity of the two key players of initiation in bacteria: the initiator protein DnaA and the origin of chromosome replication (oriC). Factors involved in the control of the availability, activity, or oligomerization of DnaA during initiation are generally regarded as the most important and thus have been thoroughly characterized. Other aspects of the initiation process, such as origin accessibility and susceptibility to unwinding, have been less explored. However, recent findings indicate that these factors have a significant role. This review focuses on DNA topology, conformation, and methylation as important factors that regulate the initiation process in bacteria. We present a comprehensive summary of the factors involved in the modulation of DNA topology, both locally at oriC and more globally at the level of the entire chromosome. We show clearly that the conformation of oriC dynamically changes, and control of this conformation constitutes another, important factor in the regulation of bacterial replication initiation. Furthermore, the process of initiation appears to be associated with the dynamics of the entire chromosome and this association is an important but largely unexplored phenomenon.
    MeSH term(s) Bacteria/genetics ; Bacterial Proteins/metabolism ; DNA Methylation ; DNA Replication ; DNA-Binding Proteins/metabolism ; Nucleic Acid Conformation ; Origin Recognition Complex/metabolism ; Protein Binding
    Chemical Substances Bacterial Proteins ; DNA-Binding Proteins ; DnaA protein, Bacteria ; OriC chromosomal replication origin ; Origin Recognition Complex
    Language English
    Publishing date 2014-06-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 80229-3
    ISSN 1089-8638 ; 0022-2836
    ISSN (online) 1089-8638
    ISSN 0022-2836
    DOI 10.1016/j.jmb.2014.04.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 867: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top