LIVIVO - Search results -

Search results

Result 1 - 10 of total 11

Search options

Article ; Online: Longitudinal evaluation of serum microRNAs as biomarkers for neuroblastoma burden and therapeutic p53 reactivation.

Van Goethem, Alan / Deleu, Jill / Yigit, Nurten / Everaert, Celine / Moreno-Smith, Myrthala / Vasudevan, Sanjeev A / Zeka, Fjoralba / Demuynck, Fleur / Barbieri, Eveline / Speleman, Frank / Mestdagh, Pieter / Shohet, Jason / Vandesompele, Jo / Van Maerken, Tom

NAR cancer

2023 Volume 5, Issue 1, Page(s) zcad002

Abstract: Accurate assessment of treatment response and residual disease is indispensable for the evaluation of cancer treatment efficacy. However, performing tissue biopsies for longitudinal follow-up poses a major challenge in the management of solid tumours ... ...

Abstract	Accurate assessment of treatment response and residual disease is indispensable for the evaluation of cancer treatment efficacy. However, performing tissue biopsies for longitudinal follow-up poses a major challenge in the management of solid tumours like neuroblastoma. In the present study, we evaluated whether circulating miRNAs are suitable to monitor neuroblastoma tumour burden and whether treatment-induced changes of miRNA abundance in the tumour are detectable in serum. We performed small RNA sequencing on longitudinally collected serum samples from mice carrying orthotopic neuroblastoma xenografts that were exposed to treatment with idasanutlin or temsirolimus. We identified 57 serum miRNAs to be differentially expressed upon xenograft tumour manifestation, out of which 21 were also found specifically expressed in the serum of human high-risk neuroblastoma patients. The murine serum levels of these 57 miRNAs correlated with tumour tissue expression and tumour volume, suggesting potential utility for monitoring tumour burden. In addition, we describe serum miRNAs that dynamically respond to p53 activation following treatment of engrafted mice with idasanutlin. We identified idasanutlin-induced serum miRNA expression changes upon one day and 11 days of treatment. By limiting to miRNAs with a tumour-related induction, we put forward hsa-miR-34a-5p as a potential pharmacodynamic biomarker of p53 activation in serum.
Language	English
Publishing date	2023-01-18
Publishing country	England
Document type	Journal Article
ISSN	2632-8674
ISSN (online)	2632-8674
DOI	10.1093/narcan/zcad002
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: RT-qPCR-based quantification of small non-coding RNAs.

Zeka, Fjoralba / Mestdagh, Pieter / Vandesompele, Jo

Methods in molecular biology (Clifton, N.J.)

2015 Volume 1296, Page(s) 85–102

Abstract: MicroRNAs (miRNAs) are small non-coding RNA molecules that negatively regulate messenger RNA (mRNA) translation into protein. MiRNAs play a key role in gene expression regulation, and their involvement in disease biology is well documented. This has ... ...

Abstract	MicroRNAs (miRNAs) are small non-coding RNA molecules that negatively regulate messenger RNA (mRNA) translation into protein. MiRNAs play a key role in gene expression regulation, and their involvement in disease biology is well documented. This has fueled the development of numerous tools for the quantification of miRNA expression levels. These tools are based on three technologies: (microarray) probe hybridization, RNA sequencing, and reverse transcription quantitative polymerase chain reaction (RT-qPCR). In this chapter, we describe a quantification system based on RT-qPCR technology, which is currently considered as the most sensitive, flexible, and accurate method for quantification of not only miRNA but also RNA expression in general. To this purpose, we have divided the protocol in three sections: reverse transcription (RT) reaction, optional preamplification (PA), and finally qPCR. Three quality-control (QC) steps are implemented in this workflow for assessment of RNA extraction efficiency, sample purity (e.g., absence of inhibitors), and inter-run variations, by examining the detection level of different spike-in synthetic miRNAs. We conclude by demonstrating raw data preprocessing and normalization using expression data obtained from high-throughput miRNA profiling of human RNA samples.
MeSH term(s)	Humans ; MicroRNAs/analysis ; MicroRNAs/genetics ; Oligonucleotides/genetics ; Quality Control ; Reverse Transcriptase Polymerase Chain Reaction/methods
Chemical Substances	MicroRNAs ; Oligonucleotides
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-2547-6_9
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples.

Zeka, Fjoralba / Vanderheyden, Katrien / De Smet, Els / Cuvelier, Claude A / Mestdagh, Pieter / Vandesompele, Jo

Scientific reports

2016 Volume 6, Page(s) 21418

Abstract: Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms ...

Abstract	Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms of reverse transcription polymerase chain reaction (RT-qPCR) sensitivity by measuring 48 protein coding genes on eight duplicate cultured cancer cell pellet FFPE samples and twenty cancer tissue FFPE samples. More intact RNA modestly increased gene detection sensitivity by 1.6 fold (earlier detection by 0.7 PCR cycles, 95% CI = 0.593-0.850). Application of gene-specific priming instead of whole transcriptome priming during reverse transcription further improved RT-qPCR sensitivity by a considerable 4.0 fold increase (earlier detection by 2.0 PCR cycles, 95% CI = 1.73-2.32). Targeted cDNA preamplification resulted in the strongest increase of RT-qPCR sensitivity and enabled earlier detection by an average of 172.4 fold (7.43 PCR cycles, 95% CI = 6.83-7.05). We conclude that gene-specific reverse transcription and targeted cDNA preamplification are adequate methods for accurate and sensitive RT-qPCR based gene expression analysis of FFPE material. The presented methods do not involve expensive or complex procedures and can be easily implemented in any routine RT-qPCR practice.
MeSH term(s)	DNA, Complementary/genetics ; DNA, Complementary/isolation & purification ; Formaldehyde ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Paraffin Embedding ; Protein Biosynthesis/genetics ; RNA/genetics ; RNA/isolation & purification ; Real-Time Polymerase Chain Reaction/methods ; Tissue Fixation/methods
Chemical Substances	DNA, Complementary ; Formaldehyde (1HG84L3525) ; RNA (63231-63-0)
Language	English
Publishing date	2016-02-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/srep21418
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Adherence and viability of intestinal bacteria to differentiated Caco-2 cells quantified by flow cytometry.

Grootaert, Charlotte / Boon, Nico / Zeka, Fjoralba / Vanhoecke, Barbara / Bracke, Marc / Verstraete, Willy / Van de Wiele, Tom

Journal of microbiological methods

2011 Volume 86, Issue 1, Page(s) 33–41

Abstract: Recent developments in host-microbe research give rise to a growing demand for rapid and accurate methods to quantify bacterial adhesion to epithelial cells. Here, we describe a new flow cytometric method to determine the amount and viability of gut ... ...

Abstract	Recent developments in host-microbe research give rise to a growing demand for rapid and accurate methods to quantify bacterial adhesion to epithelial cells. Here, we describe a new flow cytometric method to determine the amount and viability of gut bacteria, adhered to a monolayer of differentiated cells. The latter is a more relevant epithelium model than the suspended eukaryotic cells currently used in flow cytometric protocols. During the development of the method, we monitored the adhesion potential of six bacterial species and an intestinal microbial community to Caco-2 cells. The combination of SYBR Green I/propidium iodide was more efficient than carboxyfluorescein diacetate to stain the bacterial cells. In addition, a better separation between the Caco-2 background signal and viable and dead bacteria was obtained. A precise amount of Triton X-100 was used to detach adhered bacteria from Caco-2 cells and cell debris. Yet, a limited decrease in viability was observed for the intestinal microbial community treated with Triton X-100. The flow cytometric lower detection limit for pure bacterial cultures was 3.0-4.0log/mL, whereas a 5.0-5.5log/mL detection limit was obtained in the presence of Caco-2 cell background. The latter was sufficient to quantify adhered bacteria. To the best of our knowledge, this is the first description of a flow cytometric protocol that quantifies adhesion of both pure and mixed gut microbial cultures to a differentiated monolayer of Caco-2 cells and that allows to distinguish between viable and dead adhered bacteria.
MeSH term(s)	Bacteria/cytology ; Bacteria/growth & development ; Bacterial Adhesion ; Caco-2 Cells ; Flow Cytometry/methods ; Humans ; Intestines/microbiology ; Microbial Viability ; Models, Biological
Language	English
Publishing date	2011-07
Publishing country	Netherlands
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2011.03.011
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1849: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: miRBase Tracker: keeping track of microRNA annotation changes.

Van Peer, Gert / Lefever, Steve / Anckaert, Jasper / Beckers, Anneleen / Rihani, Ali / Van Goethem, Alan / Volders, Pieter-Jan / Zeka, Fjoralba / Ongenaert, Maté / Mestdagh, Pieter / Vandesompele, Jo

Database : the journal of biological databases and curation

2014 Volume 2014

Abstract: Since 2002, information on individual microRNAs (miRNAs), such as reference names and sequences, has been stored in miRBase, the reference database for miRNA annotation. As a result of progressive insights into the miRNome and its complexity, miRBase ... ...

Abstract	Since 2002, information on individual microRNAs (miRNAs), such as reference names and sequences, has been stored in miRBase, the reference database for miRNA annotation. As a result of progressive insights into the miRNome and its complexity, miRBase underwent addition and deletion of miRNA records, changes in annotated miRNA sequences and adoption of more complex naming schemes over time. Unfortunately, miRBase does not allow straightforward assessment of these ongoing miRNA annotation changes, which has resulted in substantial ambiguity regarding miRNA identity and sequence in public literature, in target prediction databases and in content on various commercially available analytical platforms. As a result, correct interpretation, comparison and integration of miRNA study results are compromised, which we demonstrate here by assessing the impact of ignoring sequence annotation changes. To address this problem, we developed miRBase Tracker (www.mirbasetracker.org), an easy-to-use online database that keeps track of all historical and current miRNA annotation present in the miRBase database. Three basic functionalities allow researchers to keep their miRNA annotation up-to-date, reannotate analytical miRNA platforms and link published results with outdated annotation to the latest miRBase release. We expect miRBase Tracker to increase the transparency and annotation accuracy in the field of miRNA research. Database url: www.mirbasetracker.org.
MeSH term(s)	Animals ; Computational Biology/methods ; Database Management Systems ; Databases, Nucleic Acid ; Humans ; Internet ; Mice ; MicroRNAs ; Molecular Sequence Annotation ; Software
Chemical Substances	MicroRNAs
Language	English
Publishing date	2014-08-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2496706-3
ISSN	1758-0463 ; 1758-0463
ISSN (online)	1758-0463
ISSN	1758-0463
DOI	10.1093/database/bau080
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: CASP8 SNP D302H (rs1045485) is associated with worse survival in MYCN-amplified neuroblastoma patients.

Rihani, Ali / De Wilde, Bram / Zeka, Fjoralba / Laureys, Geneviève / Francotte, Nadine / Tonini, Gian Paolo / Coco, Simona / Versteeg, Rogier / Noguera, Rosa / Schulte, Johannes H / Eggert, Angelika / Stallings, Raymond L / Speleman, Frank / Vandesompele, Jo / Van Maerken, Tom

PloS one

2014 Volume 9, Issue 12, Page(s) e114696

Abstract: Background: Neuroblastoma is a pediatric cancer that exhibits a wide clinical spectrum ranging from spontaneous regression in low-risk patients to fatal disease in high-risk patients. The identification of single nucleotide polymorphisms (SNPs) may help ...

Abstract	Background: Neuroblastoma is a pediatric cancer that exhibits a wide clinical spectrum ranging from spontaneous regression in low-risk patients to fatal disease in high-risk patients. The identification of single nucleotide polymorphisms (SNPs) may help explain the heterogeneity of neuroblastoma and assist in identifying patients at higher risk for poor survival. SNPs in the TP53 pathway are of special importance, as several studies have reported associations between TP53 pathway SNPs and cancer. Of note, less than 2% of neuroblastoma tumors have a TP53 mutation at diagnosis. Patients and methods: We selected 21 of the most frequently studied SNPs in the TP53 pathway and evaluated their association with outcome in 500 neuroblastoma patients using TaqMan allelic discrimination assays. Results and conclusion: We investigated the impact of 21 SNPs on overall survival, event-free survival, age at diagnosis, MYCN status, and stage of the disease in 500 neuroblastoma patients. A missense SNP in exon 10 of the CASP8 gene SNP D302H was associated with worse overall and event-free survival in patients with MYCN-amplified neuroblastoma tumors.
MeSH term(s)	Caspase 8/genetics ; Disease-Free Survival ; Gene Amplification ; Gene Expression Regulation, Neoplastic ; Genotyping Techniques ; Humans ; Infant ; N-Myc Proto-Oncogene Protein ; Neoplasm Staging ; Neuroblastoma/diagnosis ; Neuroblastoma/genetics ; Neuroblastoma/pathology ; Nuclear Proteins/genetics ; Oncogene Proteins/genetics ; Polymorphism, Single Nucleotide
Chemical Substances	MYCN protein, human ; N-Myc Proto-Oncogene Protein ; Nuclear Proteins ; Oncogene Proteins ; CASP8 protein, human (EC 3.4.22.-) ; Caspase 8 (EC 3.4.22.-)
Language	English
Publishing date	2014-12-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0114696
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Lack of association between MDM2 promoter SNP309 and clinical outcome in patients with neuroblastoma.

Rihani, Ali / Van Maerken, Tom / De Wilde, Bram / Zeka, Fjoralba / Laureys, Geneviève / Norga, Koen / Tonini, Gian Paolo / Coco, Simona / Versteeg, Rogier / Noguera, Rosa / Schulte, Johannes H / Eggert, Angelika / Stallings, Raymond L / Speleman, Frank / Vandesompele, Jo

Pediatric blood & cancer

2014 Volume 61, Issue 10, Page(s) 1867–1870

Abstract: While a polymorphism located within the promoter region of the MDM2 proto-oncogene, SNP309 (T > G), has previously been associated with increased risk and aggressiveness of neuroblastoma and other tumor entities, a protective effect has also been ... ...

Abstract	While a polymorphism located within the promoter region of the MDM2 proto-oncogene, SNP309 (T > G), has previously been associated with increased risk and aggressiveness of neuroblastoma and other tumor entities, a protective effect has also been reported in certain other cancers. In this study, we evaluated the association of MDM2 SNP309 with outcome in 496 patients with neuroblastoma and its effect on MDM2 expression. No significant difference in overall or event-free survival was observed among patients with neuroblastoma with or without MDM2 SNP309. The presence of SNP309 does not affect MDM2 expression in neuroblastoma.
MeSH term(s)	Disease-Free Survival ; Genetic Predisposition to Disease ; Genotype ; Humans ; Kaplan-Meier Estimate ; Neuroblastoma/genetics ; Neuroblastoma/mortality ; Polymorphism, Single Nucleotide ; Prognosis ; Promoter Regions, Genetic/genetics ; Proto-Oncogene Proteins c-mdm2/genetics
Chemical Substances	MDM2 protein, human (EC 2.3.2.27) ; Proto-Oncogene Proteins c-mdm2 (EC 2.3.2.27)
Language	English
Publishing date	2014-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2131448-2
ISSN	1545-5017 ; 1545-5009
ISSN (online)	1545-5017
ISSN	1545-5009
DOI	10.1002/pbc.24927
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1131: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Circulating microRNA biomarkers for metastatic disease in neuroblastoma patients.

Zeka, Fjoralba / Decock, Anneleen / Van Goethem, Alan / Vanderheyden, Katrien / Demuynck, Fleur / Lammens, Tim / Helsmoortel, Hetty H / Vermeulen, Joëlle / Noguera, Rosa / Berbegall, Ana P / Combaret, Valérie / Schleiermacher, Gudrun / Laureys, Geneviève / Schramm, Alexander / Schulte, Johannes H / Rahmann, Sven / Bienertová-Vašků, Julie / Mazánek, Pavel / Jeison, Marta /

Ash, Shifra / Hogarty, Michael D / Moreno-Smith, Mirthala / Barbieri, Eveline / Shohet, Jason / Berthold, Frank / Van Maerken, Tom / Speleman, Frank / Fischer, Matthias / De Preter, Katleen / Mestdagh, Pieter / Vandesompele, Jo

JCI insight

2018 Volume 3, Issue 23

Abstract: In this study, the circulating miRNome from diagnostic neuroblastoma serum was assessed for identification of noninvasive biomarkers with potential in monitoring metastatic disease. After determining the circulating neuroblastoma miRNome, 743 miRNAs were ...

Abstract	In this study, the circulating miRNome from diagnostic neuroblastoma serum was assessed for identification of noninvasive biomarkers with potential in monitoring metastatic disease. After determining the circulating neuroblastoma miRNome, 743 miRNAs were screened in 2 independent cohorts of 131 and 54 patients. Evaluation of serum miRNA variance in a model testing for tumor stage, MYCN status, age at diagnosis, and overall survival revealed tumor stage as the most significant factor impacting miRNA abundance in neuroblastoma serum. Differential abundance analysis between patients with metastatic and localized disease revealed 9 miRNAs strongly associated with metastatic stage 4 disease in both patient cohorts. Increasing levels of these miRNAs were also observed in serum from xenografted mice bearing human neuroblastoma tumors. Moreover, murine serum miRNA levels were strongly associated with tumor volume. These findings were validated in longitudinal serum samples from metastatic neuroblastoma patients, where the 9 miRNAs were associated with disease burden and treatment response.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Biomarkers, Tumor/blood ; Child ; Child, Preschool ; Circulating MicroRNA/blood ; Female ; Humans ; Male ; Mice ; MicroRNAs/blood ; Middle Aged ; Neoplasm Metastasis/diagnosis ; Neoplasm Staging ; Neuroblastoma/blood ; Neuroblastoma/diagnosis ; Transplantation, Heterologous ; Young Adult
Chemical Substances	Biomarkers, Tumor ; Circulating MicroRNA ; MIRN92 microRNA, human ; MicroRNAs
Language	English
Publishing date	2018-12-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2379-3708
ISSN (online)	2379-3708
DOI	10.1172/jci.insight.97021
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: miRNA expression profiling enables risk stratification in archived and fresh neuroblastoma tumor samples.

De Preter, Katleen / Mestdagh, Pieter / Vermeulen, Joëlle / Zeka, Fjoralba / Naranjo, Arlene / Bray, Isabella / Castel, Victoria / Chen, Caifu / Drozynska, Elzbieta / Eggert, Angelika / Hogarty, Michael D / Izycka-Swieszewska, Ewa / London, Wendy B / Noguera, Rosa / Piqueras, Marta / Bryan, Kenneth / Schowe, Benjamin / van Sluis, Peter / Molenaar, Jan J /

Schramm, Alexander / Schulte, Johannes H / Stallings, Raymond L / Versteeg, Rogier / Laureys, Geneviève / Van Roy, Nadine / Speleman, Frank / Vandesompele, Jo

Clinical cancer research : an official journal of the American Association for Cancer Research

2011 Volume 17, Issue 24, Page(s) 7684–7692

Abstract: Purpose: More accurate assessment of prognosis is important to further improve the choice of risk-related therapy in neuroblastoma (NB) patients. In this study, we aimed to establish and validate a prognostic miRNA signature for children with NB and ... ...

Abstract	Purpose: More accurate assessment of prognosis is important to further improve the choice of risk-related therapy in neuroblastoma (NB) patients. In this study, we aimed to establish and validate a prognostic miRNA signature for children with NB and tested it in both fresh frozen and archived formalin-fixed paraffin-embedded (FFPE) samples. Experimental design: Four hundred-thirty human mature miRNAs were profiled in two patient subgroups with maximally divergent clinical courses. Univariate logistic regression analysis was used to select miRNAs correlating with NB patient survival. A 25-miRNA gene signature was built using 51 training samples, tested on 179 test samples, and validated on an independent set of 304 fresh frozen tumor samples and 75 archived FFPE samples. Results: The 25-miRNA signature significantly discriminates the test patients with respect to progression-free and overall survival (P < 0.0001), both in the overall population and in the cohort of high-risk patients. Multivariate analysis indicates that the miRNA signature is an independent predictor of patient survival after controlling for current risk factors. The results were confirmed in an external validation set. In contrast to a previously published mRNA classifier, the 25-miRNA signature was found to be predictive for patient survival in a set of 75 FFPE neuroblastoma samples. Conclusions: In this study, we present the largest NB miRNA expression study so far, including more than 500 NB patients. We established and validated a robust miRNA classifier, able to identify a cohort of high-risk NB patients at greater risk for adverse outcome using both fresh frozen and archived material.
MeSH term(s)	Case-Control Studies ; Child ; Child, Preschool ; Cohort Studies ; Follow-Up Studies ; Gene Expression Profiling/methods ; Humans ; Infant ; Kaplan-Meier Estimate ; Logistic Models ; MicroRNAs/genetics ; Multivariate Analysis ; Neuroblastoma/diagnosis ; Neuroblastoma/genetics ; Neuroblastoma/therapy ; Prognosis ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; Risk Assessment ; Risk Factors ; Time Factors
Chemical Substances	MicroRNAs
Language	English
Publishing date	2011-10-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1225457-5
ISSN	1557-3265 ; 1078-0432
ISSN (online)	1557-3265
ISSN	1078-0432
DOI	10.1158/1078-0432.CCR-11-0610
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4223: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The need for transparency and good practices in the qPCR literature.

Bustin, Stephen A / Benes, Vladimir / Garson, Jeremy / Hellemans, Jan / Huggett, Jim / Kubista, Mikael / Mueller, Reinhold / Nolan, Tania / Pfaffl, Michael W / Shipley, Gregory / Wittwer, Carl T / Schjerling, Peter / Day, Philip J / Abreu, Mónica / Aguado, Begoña / Beaulieu, Jean-François / Beckers, Anneleen / Bogaert, Sara / Browne, John A /

Carrasco-Ramiro, Fernando / Ceelen, Liesbeth / Ciborowski, Kate / Cornillie, Pieter / Coulon, Stephanie / Cuypers, Ann / De Brouwer, Sara / De Ceuninck, Leentje / De Craene, Jurgen / De Naeyer, Hélène / De Spiegelaere, Ward / Deckers, Kato / Dheedene, Annelies / Durinck, Kaat / Ferreira-Teixeira, Margarida / Fieuw, Annelies / Gallup, Jack M / Gonzalo-Flores, Sandra / Goossens, Karen / Heindryckx, Femke / Herring, Elizabeth / Hoenicka, Hans / Icardi, Laura / Jaggi, Rolf / Javad, Farzad / Karampelias, Michael / Kibenge, Frederick / Kibenge, Molly / Kumps, Candy / Lambertz, Irina / Lammens, Tim / Markey, Amelia / Messiaen, Peter / Mets, Evelien / Morais, Sofia / Mudarra-Rubio, Alberto / Nakiwala, Justine / Nelis, Hilde / Olsvik, Pal A / Pérez-Novo, Claudina / Plusquin, Michelle / Remans, Tony / Rihani, Ali / Rodrigues-Santos, Paulo / Rondou, Pieter / Sanders, Rebecca / Schmidt-Bleek, Katharina / Skovgaard, Kerstin / Smeets, Karen / Tabera, Laura / Toegel, Stefan / Van Acker, Tim / Van den Broeck, Wim / Van der Meulen, Joni / Van Gele, Mireille / Van Peer, Gert / Van Poucke, Mario / Van Roy, Nadine / Vergult, Sarah / Wauman, Joris / Tshuikina-Wiklander, Marina / Willems, Erik / Zaccara, Sara / Zeka, Fjoralba / Vandesompele, Jo

Nature methods

2013 Volume 10, Issue 11, Page(s) 1063–1067

Abstract: Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that ... ...

Abstract	Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
MeSH term(s)	Data Collection ; Information Services ; Polymerase Chain Reaction/methods
Language	English
Publishing date	2013-11-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2169522-2
ISSN	1548-7105 ; 1548-7091
ISSN (online)	1548-7105
ISSN	1548-7091
DOI	10.1038/nmeth.2697
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 6022: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito