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Article ; Online: The AP2/ERF transcription factor MdDREB2A regulates nitrogen utilisation and sucrose transport under drought stress.

Zhang, Ting-Ting / Lin, Yu-Jing / Liu, Hao-Feng / Liu, Ya-Qi / Zeng, Zhi-Feng / Lu, Xiao-Yan / Li, Xue-Wei / Zhang, Zhen-Lu / Zhang, Shuai / You, Chun-Xiang / Guan, Qing-Mei / Lang, Zhao-Bo / Wang, Xiao-Fei

Plant, cell & environment

2024 Volume 47, Issue 5, Page(s) 1668–1684

Abstract: Drought stress is one of the main environmental factors limiting plant growth and development. Plants adapt to changing soil moisture by modifying root architecture, inducing stomatal closure, and inhibiting shoot growth. The AP2/ERF transcription factor ...

Abstract	Drought stress is one of the main environmental factors limiting plant growth and development. Plants adapt to changing soil moisture by modifying root architecture, inducing stomatal closure, and inhibiting shoot growth. The AP2/ERF transcription factor DREB2A plays a key role in maintaining plant growth in response to drought stress, but the molecular mechanism underlying this process remains to be elucidated. Here, it was found that overexpression of MdDREB2A positively regulated nitrogen utilisation by interacting with DRE cis-elements of the MdNIR1 promoter. Meanwhile, MdDREB2A could also directly bind to the promoter of MdSWEET12, which may enhance root development and nitrogen assimilation, ultimately promoting plant growth. Overall, this regulatory mechanism provides an idea for plants in coordinating with drought tolerance and nitrogen assimilation to maintain optimal plant growth and development under drought stress.
MeSH term(s)	Plant Proteins/genetics ; Plant Proteins/metabolism ; Droughts ; Plants, Genetically Modified/metabolism ; Promoter Regions, Genetic ; Sucrose/metabolism ; Gene Expression Regulation, Plant ; Stress, Physiological/genetics
Chemical Substances	Plant Proteins ; Sucrose (57-50-1)
Language	English
Publishing date	2024-01-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	391893-2
ISSN	1365-3040 ; 0140-7791
ISSN (online)	1365-3040
ISSN	0140-7791
DOI	10.1111/pce.14834
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts.

Zhu, Feng / Yuan, Jian / Li, Hong-Jian / Zeng, Zhi-Feng / Luo, Zhi-Wen / Li, Shi-Qian / He, Chi-Qiang / Jia, Xue-Fang / Zhang, Xin / Zuo, Hui / Liu, Yi-Min / Chang, Martin / Li, Yue-Qin / Zhou, Tian-Hong

Archives of virology

2016 Volume 161, Issue 5, Page(s) 1273–1284

Abstract: Despite recent results of deletion experiments showing that open reading frame (ORF) UL49 of human cytomegalovirus (HCMV) is essential, the expression, function and functional location of its encoded protein remain unknown. We generated an antibody ... ...

Abstract	Despite recent results of deletion experiments showing that open reading frame (ORF) UL49 of human cytomegalovirus (HCMV) is essential, the expression, function and functional location of its encoded protein remain unknown. We generated an antibody specific for pUL49 to investigate the protein product encoded by the UL49 ORF and identified its function in HCMV-infected host foreskin fibroblasts. A bacterial artificial chromosome (BAC) of HCMV strain Towne (pRV-Towne) and the UL49-deleted mutant pRV-delUL49Towne were used to observe virus growth by plaque assay. Using a UL49-protein-binding antibody, we located pUL49 in the fibroblast cytoplasm. pUL49 exhibited expression kinetics resembling those of the class β-2 proteins and was detected in the virion tegument. Following deletion of UL49 ORF, the virus failed to replicate, but it could be recovered by addition of pUL49 from pCDNA3.1 (+)-UL49. Our findings indicate that UL49 ORF is essential for HCMV replication in host foreskin fibroblasts.
MeSH term(s)	Base Sequence ; Cell Line ; Cytomegalovirus/genetics ; Cytomegalovirus/growth & development ; Cytomegalovirus/physiology ; Cytomegalovirus Infections/virology ; Fibroblasts/virology ; Foreskin/cytology ; Foreskin/virology ; Gene Expression Regulation, Viral/physiology ; Humans ; Male ; Microscopy, Fluorescence ; Molecular Sequence Data ; Viral Proteins/genetics ; Viral Proteins/physiology ; Virion/growth & development ; Virion/physiology ; Virus Replication/genetics ; Virus Replication/physiology
Chemical Substances	UL49 protein, human cytomegalovirus ; Viral Proteins
Language	English
Publishing date	2016-05
Publishing country	Austria
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-016-2780-4
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Article ; Online: Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P.

Zhang, WenJun / Li, HongJian / Li, YueQin / Zeng, ZhiFeng / Li, ShiQian / Zhang, Xin / Zou, Yi / Zhou, TianHong

Virology journal

2010 Volume 7, Page(s) 100

Abstract: Background: Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that typically causes asymptomatic infections in healthy individuals but may lead to serious complications in newborns and immunodeficient individuals. The emergence of drug-resistant ... ...

Abstract	Background: Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that typically causes asymptomatic infections in healthy individuals but may lead to serious complications in newborns and immunodeficient individuals. The emergence of drug-resistant strains of HCMV has posed a need for the development of new drugs and treatment strategies. Antisense molecules are promising gene-targeting agents for specific regulation of gene expression. External guide sequences (EGSs) are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. The UL49-deletion BAC of HCMV was significantly defective in growth in human foreskin fibroblasts. Therefore, UL49 gene may serve as a potential target for novel drug development to combat HCMV infection. In this study, DNA-based EGS molecules were synthesized to target the UL49 mRNA of human cytomegalovirus (HCMV). Results: By cleavage activity assessing in vitro, the EGS aimed to the cleavage site 324 nt downstream from the translational initiation codon of UL49 mRNA (i.e. EGS324) was confirmed be efficient to direct human RNase P to cleave the target mRNA sequence. When EGS324 was exogenously administered into HCMV-infected human foreskin fibroblasts (HFFs), a significant reduction of ~76% in the mRNA and ~80% in the protein expression of UL49 gene, comparing with the cells transfected with control EGSs. Furthermore, a reduction of about 330-fold in HCMV growth were observed in HCMV-infected HFFs treated with the EGS. Conclusions: These results indicated that UL49 gene was essential for replication of HCMV. Moreover, our study provides evidence that exogenous administration of a DNA-based EGS can be used as a potential therapeutic approach for inhibiting gene expression and replication of a human virus.
MeSH term(s)	Cell Line ; Cytomegalovirus/drug effects ; Cytomegalovirus/genetics ; Cytomegalovirus/physiology ; Cytomegalovirus Infections/enzymology ; Cytomegalovirus Infections/virology ; Down-Regulation/drug effects ; Gene Expression Regulation, Viral/drug effects ; Humans ; Oligonucleotides/chemical synthesis ; Oligonucleotides/genetics ; Oligonucleotides/pharmacology ; Ribonuclease P/metabolism ; Viral Structural Proteins/genetics ; Viral Structural Proteins/metabolism ; Virus Replication/drug effects
Chemical Substances	Oligonucleotides ; Viral Structural Proteins ; Ribonuclease P (EC 3.1.26.5)
Language	English
Publishing date	2010-05-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1743-422X
ISSN (online)	1743-422X
DOI	10.1186/1743-422X-7-100
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P

Zhang, WenJun / Li, HongJian / Li, YueQin / Zeng, ZhiFeng / Li, ShiQian / Zhang, Xin / Zou, Yi / Zhou, TianHong

Virology journal. 2010 Dec., v. 7, no. 1

2010

Abstract: BACKGROUND: Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that typically causes asymptomatic infections in healthy individuals but may lead to serious complications in newborns and immunodeficient individuals. The emergence of drug-resistant ... ...

Abstract	BACKGROUND: Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that typically causes asymptomatic infections in healthy individuals but may lead to serious complications in newborns and immunodeficient individuals. The emergence of drug-resistant strains of HCMV has posed a need for the development of new drugs and treatment strategies. Antisense molecules are promising gene-targeting agents for specific regulation of gene expression. External guide sequences (EGSs) are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. The UL49-deletion BAC of HCMV was significantly defective in growth in human foreskin fibroblasts. Therefore, UL49 gene may serve as a potential target for novel drug development to combat HCMV infection. In this study, DNA-based EGS molecules were synthesized to target the UL49 mRNA of human cytomegalovirus (HCMV). RESULTS: By cleavage activity assessing in vitro, the EGS aimed to the cleavage site 324 nt downstream from the translational initiation codon of UL49 mRNA (i.e. EGS324) was confirmed be efficient to direct human RNase P to cleave the target mRNA sequence. When EGS324 was exogenously administered into HCMV-infected human foreskin fibroblasts (HFFs), a significant reduction of ~76% in the mRNA and ~80% in the protein expression of UL49 gene, comparing with the cells transfected with control EGSs. Furthermore, a reduction of about 330-fold in HCMV growth were observed in HCMV-infected HFFs treated with the EGS. CONCLUSIONS: These results indicated that UL49 gene was essential for replication of HCMV. Moreover, our study provides evidence that exogenous administration of a DNA-based EGS can be used as a potential therapeutic approach for inhibiting gene expression and replication of a human virus.
Keywords	Human betaherpesvirus 5 ; drug development ; drug resistance ; fibroblasts ; gene expression ; gene expression regulation ; gene targeting ; humans ; oligonucleotides ; protein synthesis ; ribonucleases ; start codon ; therapeutics ; vertebrate viruses ; virus replication
Language	English
Dates of publication	2010-12
Size	p. 100.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2160640-7
ISSN	1743-422X
ISSN	1743-422X
DOI	10.1186/1743-422X-7-100
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P

Zhang Xin / Li ShiQian / Zeng ZhiFeng / Li YueQin / Li HongJian / Zhang WenJun / Zou Yi / Zhou TianHong

Virology Journal, Vol 7, Iss 1, p

2010 Volume 100

Abstract: Abstract Background Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that typically causes asymptomatic infections in healthy individuals but may lead to serious complications in newborns and immunodeficient individuals. The emergence of drug- ... ...

Abstract	Abstract Background Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that typically causes asymptomatic infections in healthy individuals but may lead to serious complications in newborns and immunodeficient individuals. The emergence of drug-resistant strains of HCMV has posed a need for the development of new drugs and treatment strategies. Antisense molecules are promising gene-targeting agents for specific regulation of gene expression. External guide sequences (EGSs) are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA. The UL49-deletion BAC of HCMV was significantly defective in growth in human foreskin fibroblasts. Therefore, UL49 gene may serve as a potential target for novel drug development to combat HCMV infection. In this study, DNA-based EGS molecules were synthesized to target the UL49 mRNA of human cytomegalovirus (HCMV). Results By cleavage activity assessing in vitro , the EGS aimed to the cleavage site 324 nt downstream from the translational initiation codon of UL49 mRNA (i.e. EGS324) was confirmed be efficient to direct human RNase P to cleave the target mRNA sequence. When EGS324 was exogenously administered into HCMV-infected human foreskin fibroblasts (HFFs), a significant reduction of ~76% in the mRNA and ~80% in the protein expression of UL49 gene, comparing with the cells transfected with control EGSs. Furthermore, a reduction of about 330-fold in HCMV growth were observed in HCMV-infected HFFs treated with the EGS. Conclusions These results indicated that UL49 gene was essential for replication of HCMV. Moreover, our study provides evidence that exogenous administration of a DNA-based EGS can be used as a potential therapeutic approach for inhibiting gene expression and replication of a human virus.
Keywords	Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	570
Language	English
Publishing date	2010-05-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: [A prospective multicenter clinical control trial on treatment of refractory nephrotic syndrome with mycophenolate mofetil in children].

Yi, Zhu-Wen / Wu, Xiao-Chuan / Xu, Hong / Zhou, Li-Jun / Wu, Yu-Bin / Feng, Shi-Pin / Zhou, Jian-Hua / Yang, Qing / Zhu, Guang-Hua / Liu, Ai-Min / Wei, Min-Jiang / Yu, Li / Zeng, Zhi-Feng

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

2008 Volume 10, Issue 5, Page(s) 575–578

Abstract: Objective: To evaluate the efficacy and safety of mycophenolate mofetil (MMF) plus prednisone on refractory nephrotic syndrome (RNS) in children.: Methods: One hundred and forty-two children with RNS from ten clinical trial centers were divided into ... ...

Abstract	Objective: To evaluate the efficacy and safety of mycophenolate mofetil (MMF) plus prednisone on refractory nephrotic syndrome (RNS) in children. Methods: One hundred and forty-two children with RNS from ten clinical trial centers were divided into two groups: MMF (n=87) and control (n=55). The MMF group patients were administered with oral MMF (30-40 mg/kg daily) for at least 6 months. Afterwards the patients who responded to MMF received another 6 months MMF treatment at a dosage of 10-20 mg/kg daily. The controls were treated with pulse intravenous infusion of cyclophosphamide (CTX) (10 mg/kg daily) for 2 days every 2 weeks for 3 months. Then CTX was administered at a dosage of 500 mg/m2 once a month 4, 7 and 10 months after treatment. While the patients received MMF or CTX treatment, they were treated with oral prednisone (0.5-1 mg/kg daily) for 2 to 3 months, and then the dosage of prednisone was gradually reduced. Urinary protein, liver and renal functions, and side effects of drugs were examined at regular intervals for one year. Results: Of the 87 patients, 58 achieved complete remission, 16 achieved partial remission, 9 achieved early remission and 4 had no response to treatment. In the control group, 35 achieved complete remission, 9 achieved partial remission, 1 achieved early remission and 10 had no response to treatment. The total remission rate in the MMF group (95.4%) was significantly higher than that in the control group (81.8%) (P<0.01). After treatment 67 patients (65.4%) in the MMF group had negative proteinuria compared with 36 patients (65.4%) in the control group (P>0.05). MMF was found to be more effective in reducing proteinuria, and improving hypoproteinemia, oliguria, hyperlipemia, and edema than CTX. MMF was better tolerated with lower incidences of adverse reactions than CTX. Conclusions: The combined therapy of MMF and prednisone is more effective and tolerable than pulse intravenous infusion of CTX for treatment of RNS in children.
MeSH term(s)	Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Immunosuppressive Agents/therapeutic use ; Infant ; Male ; Mycophenolic Acid/adverse effects ; Mycophenolic Acid/analogs & derivatives ; Mycophenolic Acid/therapeutic use ; Nephrotic Syndrome/drug therapy ; Prednisone/therapeutic use ; Prospective Studies
Chemical Substances	Immunosuppressive Agents ; Mycophenolic Acid (HU9DX48N0T) ; Prednisone (VB0R961HZT)
Language	Chinese
Publishing date	2008-10
Publishing country	China
Document type	Controlled Clinical Trial ; Journal Article ; Multicenter Study
ISSN	1008-8830
ISSN	1008-8830
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts

Archives of virology

Volume v. 161,, Issue no. 5

Abstract	Despite recent results of deletion experiments showing that open reading frame (ORF) UL49 of human cytomegalovirus (HCMV) is essential, the expression, function and functional location of its encoded protein remain unknown. We generated an antibody specific for pUL49 to investigate the protein product encoded by the UL49 ORF and identified its function in HCMV-infected host foreskin fibroblasts. A bacterial artificial chromosome (BAC) of HCMV strain Towne (pRV-Towne) and the UL49-deleted mutant pRV-delUL49Towne were used to observe virus growth by plaque assay. Using a UL49-protein-binding antibody, we located pUL49 in the fibroblast cytoplasm. pUL49 exhibited expression kinetics resembling those of the class β-2 proteins and was detected in the virion tegument. Following deletion of UL49 ORF, the virus failed to replicate, but it could be recovered by addition of pUL49 from pCDNA3.1 (+)-UL49. Our findings indicate that UL49 ORF is essential for HCMV replication in host foreskin fibroblasts.
Keywords	virion ; open reading frames ; microbial growth ; fibroblasts ; antibodies ; cytoplasm ; viruses ; Human herpesvirus 5 ; bacterial artificial chromosomes ; proteins ; mutants
Language	English
Document type	Article
ISSN	0304-8608
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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Article ; Online: The AP2/ERF transcription factor MdDREB2A regulates nitrogen utilisation and sucrose transport under drought stress.

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Article ; Online: Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts.

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Article ; Online: Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P.

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Article: Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P

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Article ; Online: Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P

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Article: [A prospective multicenter clinical control trial on treatment of refractory nephrotic syndrome with mycophenolate mofetil in children].

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Article: Human cytomegalovirus UL49 encodes an early, virion-associated protein essential for virus growth in human foreskin fibroblasts

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