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Article ; Online: Targeted paclitaxel prodrug nanoassemblies to improve therapeutic effects for liver cancer

Qin, Shasha / Li, Jiamin / Pan, Zhiling / Wang, Can / Zhang, Bing-Feng

Colloids and Surfaces B: Biointerfaces. 2023 June, v. 226 p.113285-

2023

Abstract: Prodrug nanoassemblies fabricated by anticancer drug conjugates exhibited more advantages in controlled drug release and bioavailability and favorable antitumor efficacy. In this paper, lactobionic acid (LA) was connected with polyethylene glycol through ...

Abstract	Prodrug nanoassemblies fabricated by anticancer drug conjugates exhibited more advantages in controlled drug release and bioavailability and favorable antitumor efficacy. In this paper, lactobionic acid (LA) was connected with polyethylene glycol through amido linkages, and paclitaxel was joined with polyethylene glycol by means of ester bonds to form the prodrug copolymer LA-PEG-PTX. Then, LA-PEG-PTX was automatically assembled into LA-PEG-PTX nanoparticles (LPP NPs) by dialysis. The LPP NPs had a relatively uniform size of approximately 200 nm, a negative potential (−13.68 mV), and a spherical shape under TEM. The drug loading of LPP NPs was 3.91%, which was measured by HPLC. The in vitro release profile of LPP NPs exhibited a sustained release feature. The results of the pharmacokinetic test in rats showed that LPP NPs had higher T₁/₂ and AUC values than the control group (free PTX) and a prolonged in vivo circulation time, thus increasing the bioavailability of PTX. Remarkably, the LPP NPs were absorbed into HepG2 cells after galactose-directed internalization and enhanced cytotoxicity. Consequently, LPP NPs displayed notable antitumor activity in Kunming mice with H22 hepatocellular carcinoma. Collectively, these findings suggested that paclitaxel prodrug-based self-assembled nanoparticles were a promising alternative for improving the bioavailability and antitumor effect of PTX.
Keywords	antineoplastic activity ; antineoplastic agents ; bioavailability ; composite polymers ; cytotoxicity ; dialysis ; drug delivery systems ; hepatoma ; nanoparticles ; paclitaxel ; pharmacokinetics ; polyethylene glycol ; LA ; PEG ; PTX ; EDC ; DMAP ; NHS ; DMSO ; PP NPs ; LPP NPs ; Prodrug nanoassemblies ; Liver cancer
Language	English
Dates of publication	2023-06
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2023.113285
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Article ; Online: Targeted paclitaxel prodrug nanoassemblies to improve therapeutic effects for liver cancer.

Qin, Shasha / Li, Jiamin / Pan, Zhiling / Wang, Can / Zhang, Bing-Feng

Colloids and surfaces. B, Biointerfaces

2023 Volume 226, Page(s) 113285

Abstract	Prodrug nanoassemblies fabricated by anticancer drug conjugates exhibited more advantages in controlled drug release and bioavailability and favorable antitumor efficacy. In this paper, lactobionic acid (LA) was connected with polyethylene glycol through amido linkages, and paclitaxel was joined with polyethylene glycol by means of ester bonds to form the prodrug copolymer LA-PEG-PTX. Then, LA-PEG-PTX was automatically assembled into LA-PEG-PTX nanoparticles (LPP NPs) by dialysis. The LPP NPs had a relatively uniform size of approximately 200 nm, a negative potential (-13.68 mV), and a spherical shape under TEM. The drug loading of LPP NPs was 3.91%, which was measured by HPLC. The in vitro release profile of LPP NPs exhibited a sustained release feature. The results of the pharmacokinetic test in rats showed that LPP NPs had higher T
MeSH term(s)	Mice ; Rats ; Animals ; Paclitaxel/chemistry ; Prodrugs/pharmacology ; Prodrugs/chemistry ; Renal Dialysis ; Polyethylene Glycols/chemistry ; Liver Neoplasms/drug therapy ; Nanoparticles/chemistry ; Cell Line, Tumor
Chemical Substances	Paclitaxel (P88XT4IS4D) ; Prodrugs ; Polyethylene Glycols (3WJQ0SDW1A)
Language	English
Publishing date	2023-03-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2023.113285
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Article ; Online: Effects of centrifugation prior to pneumatic tube system transport on routine biochemical and immunological tests of susceptibility to hemolysis.

Yang, Rui-Xia / Qiu, Si-Jie / Song, Wei-Juan / Zhang, Hui / Zhang, Bing-Feng / Xu, Hua-Guo

Clinica chimica acta; international journal of clinical chemistry

2023 Volume 541, Page(s) 117242

Abstract: Background: Pneumatic tube system (PTS) may be associated with preanalytical hemolysis. The objective of this study was to evaluate the effects of PTS on biochemical and immunological tests susceptible to hemolysis and try to find ways to reduce the ... ...

Abstract	Background: Pneumatic tube system (PTS) may be associated with preanalytical hemolysis. The objective of this study was to evaluate the effects of PTS on biochemical and immunological tests susceptible to hemolysis and try to find ways to reduce the result bias caused by PTS. Methods: Laboratory parameters were compared between PTS without centrifuging group, PTS after centrifuging group, PTS with serum group, and hand-delivered (HD) group. Studies were performed to access the influence of different PTS transport frequencies on laboratory assays. Results: PTS transportation resulted in obviously increase in LDH (lactate dehydrogenase) and NSE (neuron-specific enolase) results (LDH: Bias = 17.95%, 95% confidence interval (CI) = -3.13-39.02; p < 0.001; NSE: Bias = 64.26%, 95% CI = -21.29-149.82; p < 0.001; respectively). After pre-centrifugation, no statistical difference was observed in LDH results (Bias = 2.83%, 95% CI = -13.00-18.65; p = 0.737). However, the bias of NSE still reach 19.16% (95% CI = -41.78-80.11), which exceeded the clinical acceptable range (p = 0.017). Both LDH(p = 0.931) and NSE(p > 0.999) show no statistical difference between PTS with serum group and HD group (LDH: Bias = -1.60%, 95% CI = -6.00-2.81; NSE: Bias = -3.68%, 95% CI = -11.35-3.99). Conclusion: PTS can lead to falsely increased LDH and NSE test results. Only loading the centrifuged upper serum in new tubes during PTS transport can eliminate the results bias of NSE.
MeSH term(s)	Humans ; Hemolysis ; Blood Specimen Collection/methods ; Blood Coagulation Tests ; Laboratories ; Immunologic Tests
Language	English
Publishing date	2023-02-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2023.117242
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Article ; Online: Progress of engineered bacteria for tumour therapy.

Xia, Xue / Zhang, Jing-Wen / Zhao, Bing / Zhang, Min / Chen, Zhang-Ren / Zhang, Bing-Feng / Ji, Yu-Long / Wang, Xia / Xiong, Wen-Min / Li, Jia-Wei / Lv, Qiao-Li

International immunopharmacology

2024 Volume 132, Page(s) 111935

Abstract: Finding novel therapeutic modalities, improving drug delivery efficiency and targeting, and reducing the immune escape of tumor cells are currently hot topics in the field of tumor therapy. Bacterial therapeutics have proven highly effective in ... ...

Abstract	Finding novel therapeutic modalities, improving drug delivery efficiency and targeting, and reducing the immune escape of tumor cells are currently hot topics in the field of tumor therapy. Bacterial therapeutics have proven highly effective in preventing tumor spread and recurrence, used alone or in combination with traditional therapies. In recent years, a growing number of researchers have significantly improved the targeting and penetration of bacteria by using genetic engineering technology, which has received widespread attention in the field of tumor therapy. In this paper, we provide an overview and assessment of the advancements made in the field of tumor therapy using genetically engineered bacteria. We cover three major aspects: the development of engineered bacteria, their integration with other therapeutic techniques, and the current state of clinical trials. Lastly, we discuss the limitations and challenges that are currently being faced in the utilization of engineered bacteria for tumor therapy.
MeSH term(s)	Humans ; Neoplasms/therapy ; Neoplasms/immunology ; Animals ; Genetic Engineering ; Bacteria/genetics ; Immunotherapy/methods ; Drug Delivery Systems
Language	English
Publishing date	2024-04-09
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2024.111935
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Book ; Online: Fast Pixel-Matching for Video Object Segmentation

Yu, Siyue / Xiao, Jimin / Zhang, BingFeng / Lim, Eng Gee

2021

Abstract: Video object segmentation, aiming to segment the foreground objects given the annotation of the first frame, has been attracting increasing attentions. Many state-of-the-art approaches have achieved great performance by relying on online model updating ... ...

Abstract	Video object segmentation, aiming to segment the foreground objects given the annotation of the first frame, has been attracting increasing attentions. Many state-of-the-art approaches have achieved great performance by relying on online model updating or mask-propagation techniques. However, most online models require high computational cost due to model fine-tuning during inference. Most mask-propagation based models are faster but with relatively low performance due to failure to adapt to object appearance variation. In this paper, we are aiming to design a new model to make a good balance between speed and performance. We propose a model, called NPMCA-net, which directly localizes foreground objects based on mask-propagation and non-local technique by matching pixels in reference and target frames. Since we bring in information of both first and previous frames, our network is robust to large object appearance variation, and can better adapt to occlusions. Extensive experiments show that our approach can achieve a new state-of-the-art performance with a fast speed at the same time (86.5% IoU on DAVIS-2016 and 72.2% IoU on DAVIS-2017, with speed of 0.11s per frame) under the same level comparison. Source code is available at https://github.com/siyueyu/NPMCA-net. Comment: Accepted by Signal Processing: Image Communication
Keywords	Computer Science - Computer Vision and Pattern Recognition
Subject code	004 ; 006
Publishing date	2021-07-09
Publishing country	us
Document type	Book ; Online
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Article: Preliminary Research on Syndrome Types of Chinese Medicine in Children with Primary Nephrotic Syndrome.

Sun, Wen / Yu, Jian / Zeng, Gu-Lan / Zhang, Bing-Feng

Chinese journal of integrative medicine

2017 Volume 24, Issue 8, Page(s) 579–583

Abstract: Objective: To provide an objective reference for the syndrome types of Chinese medicine (CM) associated with pediatric primary nephrotic syndrome (PNS).: Methods: A cross-sectional study was performed. Data on clinical symptoms, CM syndrome types, ... ...

Abstract	Objective: To provide an objective reference for the syndrome types of Chinese medicine (CM) associated with pediatric primary nephrotic syndrome (PNS). Methods: A cross-sectional study was performed. Data on clinical symptoms, CM syndrome types, biochemical indices, and medications used were collected from 98 children with PNS. Then, the correlation between CM syndromes and biochemical indices, as well as medications used, was analyzed. Results: The four most common symptoms in children with PNS were brown urine, red tongue, excessive sweating, and swelling of the face and limbs. The syndromes of qi deficiency of Fei (Lung) and Shen (Kidney) (FSQD) and yin deficiency of Gan (Liver) and Shen (GSYD) were the most common main CM syndrome types. FSQD syndrome score correlated significantly with the total cholesterol level, urine protein/creatinine ratio, and urine IgG and albumin levels (P<0.01 or P<0.05). The use of maintenance glucocorticoids combined with immunosuppressive agents correlated with FSQD syndrome, and the use of maintenance glucocorticoids alone correlated with GSYD syndrome (P<0.05). Conclusion: Two of the most common CM syndrome types were FSQD and GSYD syndromes. FSQD syndrome may be caused by some factors related to lipid levels, protein loss, and the use of immunosuppressive agents. The use of maintenance glucocorticoids may cause GSYD syndrome.
MeSH term(s)	Adolescent ; Child ; Child, Preschool ; Demography ; Drugs, Chinese Herbal/therapeutic use ; Female ; Humans ; Infant ; Male ; Medicine, Chinese Traditional ; Nephrotic Syndrome/drug therapy ; Nephrotic Syndrome/therapy
Chemical Substances	Drugs, Chinese Herbal
Language	English
Publishing date	2017-08-17
Publishing country	China
Document type	Journal Article
ZDB-ID	2171254-2
ISSN	1993-0402 ; 1672-0415
ISSN (online)	1993-0402
ISSN	1672-0415
DOI	10.1007/s11655-017-2961-3
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Article ; Online: Chitosan-modified cholesterol-free liposomes for improving the oral bioavailability of progesterone.

Wang, Mei / Liu, Meng / Xie, Tingting / Zhang, Bing-Feng / Gao, Xiao-Li

Colloids and surfaces. B, Biointerfaces

2017 Volume 159, Page(s) 580–585

Abstract: Based on the structurally similar properties of progesterone and cholesterol, chitosan-coated cholesterol-free liposomes (CS-Lipo/Prog) were formulated. CS-Lipo/Prog are spherical and uniform in size (662.1±19.3nm) with positive potential (28.19±1.97mV). ...

Abstract	Based on the structurally similar properties of progesterone and cholesterol, chitosan-coated cholesterol-free liposomes (CS-Lipo/Prog) were formulated. CS-Lipo/Prog are spherical and uniform in size (662.1±19.3nm) with positive potential (28.19±1.97mV). The average drug entrapment efficiency (EE) is approximately 80%. The in vitro release profile of CS-Lipo/Prog shows sustained release. The in vitro stability evaluation demonstrated that CS-Lipo/Prog can efficiently shield Prog from degradation in the gastrointestinal tract. CS-Lipo/Prog showed a longer MRT and higher AUC
MeSH term(s)	Animals ; Chitosan/chemistry ; Cholesterol/chemistry ; Gastrointestinal Tract/metabolism ; Liposomes/chemistry ; Mice ; Progesterone/chemistry
Chemical Substances	Liposomes ; Progesterone (4G7DS2Q64Y) ; Chitosan (9012-76-4) ; Cholesterol (97C5T2UQ7J)
Language	English
Publishing date	2017-11-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2017.08.028
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Article ; Online: Liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients.

Xia, Wen-Ying / Gao, Li / Dai, Er-Hei / Chen, Dan / Xie, Er-Fu / Yang, Li / Zhang, Shi-Chang / Zhang, Bing-Feng / Xu, Jian / Pan, Shi-Yang

World journal of gastroenterology

2019 Volume 25, Issue 29, Page(s) 3985–3995

Abstract: Background: Hepatitis B is a major public health problem in China. Accurate liver injury assessment is essential for clinical evidence-based treatment. Liver biopsy is considered the gold standard method to stage liver disease, but it is not widely used ...

Abstract	Background: Hepatitis B is a major public health problem in China. Accurate liver injury assessment is essential for clinical evidence-based treatment. Liver biopsy is considered the gold standard method to stage liver disease, but it is not widely used in resource-limited settings. Therefore, non-invasive liquid biopsy tests are needed. Aim: To assess liver injury in hepatitis B patients using quantified cell free DNA combined with other serum biomarker as a liquid biopsy-based method. Methods: A cohort of 663 subjects including 313 hepatitis B patients and 350 healthy controls were enrolled. Ultrasound-guided liver biopsies followed by histopathological assessments were performed for the 263 chronic hepatitis B patients to determine the degree of liver injury. Cell-free DNA was quantified using a novel duplex real-time polymerase chain reaction assay. Results: Compared with healthy controls, patients with hepatitis B virus (HBV) infection had significantly higher plasma DNA, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, and HBV DNA levels ( Conclusion: The combination of plasma DNA, serum ALT, and bilirubin could be a candidate liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients.
MeSH term(s)	Adolescent ; Adult ; Aged ; Alanine Transaminase/blood ; Bilirubin/blood ; Biomarkers/blood ; Cell-Free Nucleic Acids/blood ; China ; Cohort Studies ; Feasibility Studies ; Female ; Healthy Volunteers ; Hepatitis B, Chronic/blood ; Hepatitis B, Chronic/diagnosis ; Hepatitis B, Chronic/pathology ; Humans ; Liquid Biopsy/methods ; Liver/pathology ; Liver Function Tests/methods ; Male ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Severity of Illness Index ; Young Adult
Chemical Substances	Biomarkers ; Cell-Free Nucleic Acids ; Alanine Transaminase (EC 2.6.1.2) ; Bilirubin (RFM9X3LJ49)
Language	English
Publishing date	2019-08-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.v25.i29.3985
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Article ; Online: Glutamatergic lateral hypothalamus promotes defensive behaviors.

Chen, Li / Cai, Ping / Wang, Ren-Fei / Lu, Yi-Ping / Chen, Hui-Yun / Guo, Yu-Rou / Huang, Sheng-Nan / Hu, Li-Huan / Chen, Jian / Zheng, Ze-Hong / He, Peng / Zhang, Bing-Feng / Liu, Ji-Yuan / Wang, Wen-Xiang / Li, Huang-Yuan / Yu, Chang-Xi

Neuropharmacology

2020 Volume 178, Page(s) 108239

Abstract: The glutamatergic lateral hypothalamus (LH) has been implicated in a variety of behaviors, such as evasion and feeding, while its role in defensive behaviors and relevant neurocircuits remains unclear. Here, we demonstrated that the glutamatergic LH is a ...

Abstract	The glutamatergic lateral hypothalamus (LH) has been implicated in a variety of behaviors, such as evasion and feeding, while its role in defensive behaviors and relevant neurocircuits remains unclear. Here, we demonstrated that the glutamatergic LH is a critical structure regulating defensive behaviors. Trimethylthiazole (TMT), the odor of mice predator, significantly increased c-Fos expression in the LH. Using fiber photometry technology, we found that TMT exposure increased the activity of LH glutamatergic neurons. Selective activation of LH glutamatergic neurons with optogenetics and chemogenetics promoted a series of defense-related behaviors, including fleeing, avoidance, and hiding, while selective inhibition of LH glutamatergic neurons suppressed the avoidance provoked by TMT. Activation of both the glutamatergic LH terminals in the hypothalamic paraventricular nucleus (PVN) and the glutamatergic projection from the basolateral amygdala (BLA) to the LH elicited defensive behaviors. Finally, by combining the viral-mediated retrograde tracing with anterograde activation, we found that PVN-projecting glutamatergic neurons in the LH were activated by BLA glutamatergic inputs. Taken together, our results illustrate that the glutamatergic LH is a pivotal relay of defensive behaviors and possibly promotes these behaviors through the BLA→LH→PVN pathway.
MeSH term(s)	Animals ; Avoidance Learning/physiology ; Defense Mechanisms ; Glutamic Acid/analysis ; Glutamic Acid/metabolism ; Hypothalamic Area, Lateral/chemistry ; Hypothalamic Area, Lateral/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Optogenetics/methods
Chemical Substances	Glutamic Acid (3KX376GY7L)
Language	English
Publishing date	2020-08-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218272-5
ISSN	1873-7064 ; 0028-3908
ISSN (online)	1873-7064
ISSN	0028-3908
DOI	10.1016/j.neuropharm.2020.108239
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Article ; Online: High Uric Acid (UA) Negatively Affects Serum Tartrate-Resistant Acid Phosphatase 5b (TRACP 5b) Immunoassay.

Wu, Zhi-Qi / Zhang, Yan / Xie, Erfu / Song, Wei-Juan / Yang, Rui-Xia / Yan, Cheng-Jing / Zhang, Bing-Feng / Xu, Hua-Guo

PloS one

2016 Volume 11, Issue 1, Page(s) e0147554

Abstract: Background: Bone metastases often occur in the majority of patients with advanced cancer, such as prostate cancer, lung cancer and breast cancer. Serum tartrate-resistant acid phosphatase 5b (TRACP 5b), a novel bone resorption marker, has been used ... ...

Abstract	Background: Bone metastases often occur in the majority of patients with advanced cancer, such as prostate cancer, lung cancer and breast cancer. Serum tartrate-resistant acid phosphatase 5b (TRACP 5b), a novel bone resorption marker, has been used gradually in the clinics as a specific and sensitive marker of bone resorption for the early diagnosis of cancer patients with bone metastasis. Here, we reported that high concentrations of uric acid (UA) lead to decrease of TRACP 5b levels and determined whether TRACP 5b level was associated with UA in interference experiment. Methods: A total of 77 patients with high concentrations of UA and 77 healthy subjects were tested to evaluate the differences in their TRACP 5b levels. Serial dilutions of UA were respectively spiked with a known concentration of TRACP 5b standard sample, then Serum TRACP 5b was detected by using bone TRAP® Assay. A correction equation was set to eliminate UA-derived TRACP 5b false-decrease. The effect of this correction was evaluated in high-UA individuals. Results: The average TRACP level of the high-UA individuals (1.47 ± 0.62 U/L) was significantly lower than that of the healthy subjects (2.62 ± 0.63 U/L) (t-test, p < 0.0001). The UA correction equation derived: ΔTRACP 5b = -1.9751lgΔUA + 3.7365 with an R2 = 0.98899. Application of the UA correction equation resulted in a statistically non-significant difference in TRACP 5b values between the healthy subjects and high-UA individuals (p = 0.24). Conclusions: High UA concentrations can falsely decrease TRACP 5b levels due to a method-related systematic error. To avoid misdiagnoses or inappropriate therapeutic decisions, increased attention should be paid to UA interference, when TRACP 5b is used for early diagnosis of cancer patients with bone metastasis, evaluation of the aggressiveness of osteosarcoma or prediction of survival in prostate cancer and breast cancer with bone metastases.
MeSH term(s)	Acid Phosphatase/blood ; Adolescent ; Adult ; Aged ; Biomarkers, Tumor/blood ; Bone Neoplasms/blood ; Bone Resorption/blood ; Case-Control Studies ; False Negative Reactions ; Female ; Humans ; Immunoassay ; Isoenzymes/blood ; Male ; Middle Aged ; Neoplasms/blood ; Neoplasms/diagnosis ; Tartrate-Resistant Acid Phosphatase ; Uric Acid/blood ; Young Adult
Chemical Substances	Biomarkers, Tumor ; Isoenzymes ; Uric Acid (268B43MJ25) ; ACP5 protein, human (EC 3.1.3.2) ; Acid Phosphatase (EC 3.1.3.2) ; Tartrate-Resistant Acid Phosphatase (EC 3.1.3.2)
Language	English
Publishing date	2016-01-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0147554
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