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Article: Research status and laboratory diagnosis of the new coronavirus

Jin, Wei-dong Zhang Dong-yun Xie Feng-xin Fu Wei-ling Zhang Yang

Abstract: A review on research status and laboratory diagnosis of the new coronavirus It is urgently needed to have reliable and quick test technique for diagnosis POCT and high throughput screening biochiare suggested Protection methods for laboratory stuff are ... ...

Abstract	A review on research status and laboratory diagnosis of the new coronavirus It is urgently needed to have reliable and quick test technique for diagnosis POCT and high throughput screening biochiare suggested Protection methods for laboratory stuff are also summaried
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #2035
Database	COVID19

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Article ; Online: SP1-induced upregulation of lncRNA CTBP1-AS2 accelerates the hepatocellular carcinoma tumorigenesis through targeting CEP55 via sponging miR-195-5p.

Liu, Li-Xia / Liu, Bin / Yu, Jie / Zhang, Dong-Yun / Shi, Jian-Hong / Liang, Ping

Biochemical and biophysical research communications

2020 Volume 533, Issue 4, Page(s) 779–785

Abstract: As reported in many research, LncRNA CTBP1 divergent transcript (CTBP1-AS2) remarkably affects the progression of several tumors. However, the precise role and function of CTBP1-AS2 in hepatocellular carcinoma (HCC) remained unknown. We found that CTBP1- ... ...

Abstract	As reported in many research, LncRNA CTBP1 divergent transcript (CTBP1-AS2) remarkably affects the progression of several tumors. However, the precise role and function of CTBP1-AS2 in hepatocellular carcinoma (HCC) remained unknown. We found that CTBP1-AS2 expressions were increased in HCC samples and cells. After treatment with microwave ablation (MWA), CTBP1-AS2 was distinctly up-regulated in residual HCC tissues compared with HCC samples. CTBP1-AS2 was upregulated under the induction of the nuclear transcription factor SP1. As revealed by the clinical assays, high CTBP1-AS2 expression usually related to lymph node metastasis, clinical stage and weaker prognosis specific to HCC patients. Functionally, CTBP1-AS2 knockdown suppressed HCC cells in terms of the proliferation, migration, invasion, chemotherapy resistance as well as EMT progress, but promoted apoptosis. Mechanistically, CTBP1-AS2 was a sponge of miR-195-5p for elevating CEP55 expression, a target of miR-195-5p, and thereby exhibited its oncogenic roles in HCC progression. Overall, an emerging regulatory mechanism of SP1/CTBP1-AS2/miR-195-5p/CEP55 axis was reported in the paper, which possibly served as a new therapeutic HCC treatment target.
MeSH term(s)	Apoptosis ; Carcinogenesis/genetics ; Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/pathology ; Cell Cycle Proteins/genetics ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Humans ; Liver Neoplasms/genetics ; Liver Neoplasms/metabolism ; Liver Neoplasms/pathology ; MicroRNAs/metabolism ; Neoplasm Invasiveness ; Prognosis ; RNA, Long Noncoding/biosynthesis ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/physiology ; Sp1 Transcription Factor/metabolism ; Up-Regulation
Chemical Substances	Cell Cycle Proteins ; Cep55 protein, human ; MIRN195 microRNA, human ; MicroRNAs ; RNA, Long Noncoding ; Sp1 Transcription Factor ; SP1 protein, human
Language	English
Publishing date	2020-09-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2020.09.080
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Article: SP1-induced upregulation of lncRNA CTBP1-AS2 accelerates the hepatocellular carcinoma tumorigenesis through targeting CEP55 via sponging miR-195-5p

Liu, Li-xia / Liu, Bin / Yu, Jie / Zhang, Dong-yun / Shi, Jian-hong / Liang, Ping

Biochemical and biophysical research communications. 2020 Dec. 17, v. 533, no. 4

2020

Abstract	As reported in many research, LncRNA CTBP1 divergent transcript (CTBP1-AS2) remarkably affects the progression of several tumors. However, the precise role and function of CTBP1-AS2 in hepatocellular carcinoma (HCC) remained unknown. We found that CTBP1-AS2 expressions were increased in HCC samples and cells. After treatment with microwave ablation (MWA), CTBP1-AS2 was distinctly up-regulated in residual HCC tissues compared with HCC samples. CTBP1-AS2 was upregulated under the induction of the nuclear transcription factor SP1. As revealed by the clinical assays, high CTBP1-AS2 expression usually related to lymph node metastasis, clinical stage and weaker prognosis specific to HCC patients. Functionally, CTBP1-AS2 knockdown suppressed HCC cells in terms of the proliferation, migration, invasion, chemotherapy resistance as well as EMT progress, but promoted apoptosis. Mechanistically, CTBP1-AS2 was a sponge of miR-195-5p for elevating CEP55 expression, a target of miR-195-5p, and thereby exhibited its oncogenic roles in HCC progression. Overall, an emerging regulatory mechanism of SP1/CTBP1-AS2/miR-195-5p/CEP55 axis was reported in the paper, which possibly served as a new therapeutic HCC treatment target.
Keywords	apoptosis ; carcinogenesis ; drug therapy ; hepatoma ; lymph nodes ; metastasis ; prognosis ; research ; transcription factors
Language	English
Dates of publication	2020-1217
Size	p. 779-785.
Publishing place	Elsevier Inc.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2020.09.080
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Increased prognostic value of clinical-reproductive model in Chinese female patients with esophageal squamous cell carcinoma.

Zhang, Dong-Yun / Ku, Jian-Wei / Zhao, Xue-Ke / Zhang, Hai-Yan / Song, Xin / Wu, Hong-Fang / Fan, Zong-Min / Xu, Rui-Hua / You, Duo / Wang, Ran / Zhou, Ruo-Xi / Wang, Li-Dong

World journal of gastroenterology

2022 Volume 28, Issue 13, Page(s) 1347–1361

Abstract: Background: In China, it has been well recognized that some female patients with esophageal squamous cell carcinoma (ESCC) have different overall survival (OS) time, even with the same tumor-node-metastasis (TNM) stage, challenging the prognostic value ... ...

Abstract	Background: In China, it has been well recognized that some female patients with esophageal squamous cell carcinoma (ESCC) have different overall survival (OS) time, even with the same tumor-node-metastasis (TNM) stage, challenging the prognostic value of the TNM system alone. An effective predictive model is needed to accurately evaluate the prognosis of female ESCC patients. Aim: To construct a novel prognostic model with clinical and reproductive data for Chinese female patients with ESCC, and to assess the incremental prognostic value of the full model compared with the clinical model and TNM stage. Methods: A new prognostic nomogram incorporating clinical and reproductive features was constructed based on univariatie and Cox proportional hazards survival analysis from a training cohort ( Results: A clinical-reproductive model including incidence area, age, tumor differentiation, lymph node metastasis (N) stage, estrogen receptor alpha (ESR1) and beta (ESR2) expression, menopausal age, and pregnancy number was constructed to predict OS in female ESCC patients. Compared to the clinical model and TNM stage, the time-dependent ROC and C-index of the clinical-reproductive model showed a good discriminative ability for predicting 1-, 3-, and 5-years OS in the primary training, internal and external validation sets. Based on the optimal cut-off value of total prognostic scores, patients were classified into high- and low-risk groups with significantly different OS. The estrogen response was significantly associated with p53 and apoptosis pathways in esophageal cancer. Conclusion: The clinical-reproductive prognostic nomogram has an incremental prognostic value compared with the clinical model and TNM stage in predicting OS in Chinese female ESCC patients.
MeSH term(s)	Esophageal Neoplasms/pathology ; Esophageal Squamous Cell Carcinoma/pathology ; Estrogens ; Female ; Humans ; Neoplasm Staging ; Prognosis
Chemical Substances	Estrogens
Language	English
Publishing date	2022-05-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.v28.i13.1347
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Article: Research status and laboratory diagnosis of the new coronavirus

Jin, Wei-dong / Zhang, Dong-yun / Xie, Feng-xin / Fu, Wei-ling / Zhang, Yang

Open Access (OA) Online-First Publ. Res. Pap. COVID-19

Abstract: A review on research status and laboratory diagnosis of the new coronavirus It is urgently needed to have reliable and quick test technique for diagnosis POCT and high throughput screening biochip are suggested Protection methods for laboratory stuff are ...

Abstract	A review on research status and laboratory diagnosis of the new coronavirus It is urgently needed to have reliable and quick test technique for diagnosis POCT and high throughput screening biochip are suggested Protection methods for laboratory stuff are also summaried
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #659498
Database	COVID19

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Article: Development and validation of a prognostic nomogram model for Chinese patients with primary small cell carcinoma of the esophagus.

Zhang, Dong-Yun / Huang, Gai-Rong / Ku, Jian-Wei / Zhao, Xue-Ke / Song, Xin / Xu, Rui-Hua / Han, Wen-Li / Zhou, Fu-You / Wang, Ran / Wei, Meng-Xia / Wang, Li-Dong

World journal of clinical cases

2021 Volume 9, Issue 30, Page(s) 9011–9022

Abstract: Background: Primary small cell carcinoma of the esophagus (PSCE) is a highly invasive malignant tumor with a poor prognosis compared with esophageal squamous cell carcinoma. Due to the limited samples size and the short follow-up time, there are few ... ...

Abstract	Background: Primary small cell carcinoma of the esophagus (PSCE) is a highly invasive malignant tumor with a poor prognosis compared with esophageal squamous cell carcinoma. Due to the limited samples size and the short follow-up time, there are few reports on elucidating the prognosis of PSCE, especially on the establishment and validation of a survival prediction nomogram model covering general information, pathological factors and specific biological proteins of PSCE patients. Aim: To establish an effective nomogram to predict the overall survival (OS) probability for PSCE patients in China. Methods: The nomogram was based on a retrospective study of 256 PSCE patients. Univariate analysis and multivariate Cox proportional hazards regression analysis were used to examine the prognostic factors associated with PSCE, and establish the model for predicting 1-, 3-, and 5-year OS based on the Akaike information criterion. Discrimination and validation were assessed by the concordance index (C-index) and calibration curve and decision curve analysis (DCA). Histology type, age, tumor invasion depth, lymph node invasion, detectable metastasis, chromogranin A, and neuronal cell adhesion molecule 56 were integrated into the model. Results: The C-index was prognostically superior to the 7 Conclusion: Our nomogram can be used to predict the survival probability of PSCE patients, which can help clinicians to make individualized survival predictions.
Language	English
Publishing date	2021-11-09
Publishing country	United States
Document type	Journal Article
ISSN	2307-8960
ISSN	2307-8960
DOI	10.12998/wjcc.v9.i30.9011
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Article ; Online: Amplified intracellular Ca

Dou, Jian-Ping / Wu, Qiong / Fu, Chang-Hui / Zhang, Dong-Yun / Yu, Jie / Meng, Xian-Wei / Liang, Ping

Journal of nanobiotechnology

2019 Volume 17, Issue 1, Page(s) 118

Abstract: Background: Developing new strategies to reduce the output power of microwave (MW) ablation while keeping anti-tumor effect are highly desirable for the simultaneous achievement of effective tumor killing and avoidance of complications. We find that ... ...

Abstract	Background: Developing new strategies to reduce the output power of microwave (MW) ablation while keeping anti-tumor effect are highly desirable for the simultaneous achievement of effective tumor killing and avoidance of complications. We find that mild MW irradiation can significantly increase intracellular Ca Results: The as-made NaCl-DOX@PLGA nanoplatform selectively elevates the temperature of tumor tissue distributed with nanoparticles under low-output MW, which further prompts the release of DOX from the PLGA nanoparticles and tumor cellular uptake of DOX. More importantly, its synergistic effect not only combines thermal ablation and chemotherapy, but also obviously increases the intracellular Ca Conclusions: This work provides a biocompatible and biodegradable nanoplatform with triple functions to realize the effective tumor killing in unlethal temperature. Those findings provide reliable solution to solve the bottleneck problem bothering clinics about the balance of thermal efficiency and normal tissue protection.
MeSH term(s)	Animals ; Antibiotics, Antineoplastic/therapeutic use ; Calcium/metabolism ; Doxorubicin/therapeutic use ; Female ; Hep G2 Cells ; Humans ; Hyperthermia, Induced/methods ; Mice, Nude ; Microwaves ; Nanoparticles/therapeutic use ; Neoplasms/metabolism ; Neoplasms/pathology ; Neoplasms/therapy ; Polylactic Acid-Polyglycolic Acid Copolymer/therapeutic use
Chemical Substances	Antibiotics, Antineoplastic ; Polylactic Acid-Polyglycolic Acid Copolymer (1SIA8062RS) ; Doxorubicin (80168379AG) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2019-12-02
Publishing country	England
Document type	Journal Article
ISSN	1477-3155
ISSN (online)	1477-3155
DOI	10.1186/s12951-019-0549-0
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Article ; Online: Comparison of different gastric bypass procedures in gastric carcinoma patients with type 2 diabetes mellitus.

Xiong, Shao-Wei / Zhang, Dong-Yun / Liu, Xian-Ming / Liu, Zeng / Zhang, Fang-Ting

World journal of gastroenterology

2014 Volume 20, Issue 48, Page(s) 18427–18431

Abstract: Aim: To determine the effect of different Roux-en-Y gastric bypass procedures in gastric carcinoma patients with type 2 diabetes mellitus.: Methods: A retrospective analysis of the clinical data of 54 patients with gastric cancer and type 2 diabetes ... ...

Abstract	Aim: To determine the effect of different Roux-en-Y gastric bypass procedures in gastric carcinoma patients with type 2 diabetes mellitus. Methods: A retrospective analysis of the clinical data of 54 patients with gastric cancer and type 2 diabetes mellitus treated in the Department of General Surgery from January 2006 to June 2013 was conducted. The patients underwent gastrectomy using different Roux-en-Y gastric bypass procedures (traditional, n = 26; modified, n = 28). Fasting plasma glucose (FPG), two hour postprandial blood glucose (2 h PBG) and hemoglobin A1c (HbA1c) were analyzed before surgery (0 mo) and 1, 3 and 6 mo after surgery. Results: FPG and 2 h PBG levels were significantly decreased 1 mo after surgery in the traditional Roux-en-Y gastric bypass group (FPG 7.5 ± 1.3 vs 10.7 ± 1.2, P < 0.05) (2 h PBG 10.2 ± 1.8 vs 13.8 ± 3.2, P < 0.05). FPG and 2 h PBG levels were significantly decreased after surgery in the modified Roux-en-Y gastric bypass group (FPG 6.9 ± 1.2 vs 10.5 ± 1.1, 6.5 ± 1.3 vs 10.5 ± 1.1, 6.4 ± 1.2 vs 10.5 ± 1.1, P < 0.05) (2 h PBG 9.9 ± 2.2 vs 14.1 ± 2.9, 9.2 ± 2.4 vs 14.1 ± 2.9, 8.9 ± 2.6 vs 14.1 ± 2.9, P < 0.05). Compared with the levels before surgery, HbA1c levels were significantly decreased 3 and 6 mo after surgery (7.2 ± 1.1 vs 10.5 ± 1.1, 5.5 ± 1.1 vs 10.5 ± 1.1, P < 0.05). Significant differences between the two groups regarding FPG, 2 h PBG and HbA1c concentration were observed 3 and 6 mo after surgery (FPG 10.1 ± 1.5 vs 6.5 ± 1.3, 10.3 ± 1.4 vs 6.4 ± 1.2, P < 0.05) (2 h PBG 13.1 ± 2.8 vs 9.2 ± 2.4, 13.6 ± 3.1 vs 8.9 ± 2.6, P < 0.05) (HbA1c 10.1 ± 1.4 vs 7.2 ± 1.1, 10.5 ± 1.3 vs 5.5 ± 1.1, P < 0.05). Conclusion: Modified Roux-en-Y gastric bypass can improve glucose metabolism in type 2 diabetic patients with gastric cancer.
MeSH term(s)	Adult ; Biomarkers/blood ; Blood Glucose/metabolism ; Carcinoma/complications ; Carcinoma/pathology ; Carcinoma/surgery ; Diabetes Mellitus, Type 2/blood ; Diabetes Mellitus, Type 2/complications ; Diabetes Mellitus, Type 2/diagnosis ; Fasting/blood ; Female ; Gastrectomy/adverse effects ; Gastrectomy/methods ; Gastric Bypass/adverse effects ; Gastric Bypass/methods ; Glycated Hemoglobin A/metabolism ; Humans ; Male ; Middle Aged ; Postprandial Period ; Retrospective Studies ; Stomach Neoplasms/complications ; Stomach Neoplasms/pathology ; Stomach Neoplasms/surgery ; Time Factors ; Treatment Outcome
Chemical Substances	Biomarkers ; Blood Glucose ; Glycated Hemoglobin A ; hemoglobin A1c protein, human
Language	English
Publishing date	2014-12-28
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.v20.i48.18427
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Article: Thin-walled graphitic nanocages with nitrogen-doping as superior performance anodes for lithium-ion batteries

Hong, Cheng Yang / Sheng, Zhao Min / Hu, Ming Hui / Dai, Xian You / Chang, Cheng Kang / Chen, Qi Zhong / Zhang, Dong Yun

RSC advances. 2016 June 21, v. 6, no. 65

2016

Abstract: N-Doped nanocages with thin-walled graphitic shells (∼1.2 nm) were synthesized by removing the ferrous cores of Fe@C nanoparticles. The Fe@C nanopores were prepared by floating catalytic pyrolysis with the N atoms being in situ doped in their graphitic ... ...

Abstract	N-Doped nanocages with thin-walled graphitic shells (∼1.2 nm) were synthesized by removing the ferrous cores of Fe@C nanoparticles. The Fe@C nanopores were prepared by floating catalytic pyrolysis with the N atoms being in situ doped in their graphitic shells. Due to the thin-walled graphitic shells of the nanocages, the fast distribution of the electrolyte and ions was achieved and the prepared nanocage-based anode of lithium-ion batteries showed excellent performance: a high reversible specific capacity (after 50 cycles; 760 mA h g−1 at 0.5 A g−1) and a remarkable rate capability (after 40 cycles; 250 mA h g−1 at 10 A g−1).
Keywords	anodes ; ions ; lithium batteries ; nanoparticles ; nanopores ; pyrolysis
Language	English
Dates of publication	2016-0621
Size	p. 59896-59899.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c6ra10803b
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Studies of the defect structure for V3+ ions in wurtzite structure ZnO.

Wei, Qun / Guo, Li-Xin / Yang, Zi-Yuan / Wei, Bing / Zhang, Dong-Yun

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2011 Volume 82, Issue 1, Page(s) 137–139

Abstract: By using crystal field theory, the optical spectra, zero field splitting and g factors have been calculated. The defect structure for V(3+) in ZnO crystal has been studied by using crystal field theory and first-principle calculations. The results show ... ...

Abstract	By using crystal field theory, the optical spectra, zero field splitting and g factors have been calculated. The defect structure for V(3+) in ZnO crystal has been studied by using crystal field theory and first-principle calculations. The results show that, the V(3+) ions do not occupy the exact Zn(2+) site, but displaced along C(3) axis.
MeSH term(s)	Algorithms ; Binding Sites ; Chemistry/methods ; Crystallization ; Ions ; Iron Compounds/chemistry ; Manganese/chemistry ; Minerals/chemistry ; Models, Molecular ; Models, Statistical ; Molecular Conformation ; Sulfides/chemistry ; Vanadium/chemistry ; Zinc Oxide/chemistry
Chemical Substances	Ions ; Iron Compounds ; Minerals ; Sulfides ; Vanadium (00J9J9XKDE) ; Manganese (42Z2K6ZL8P) ; Zinc Oxide (SOI2LOH54Z)
Language	English
Publishing date	2011-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2011.07.016
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