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  1. Article ; Online: Cross-modality integration framework with prediction, perception and discrimination for video anomaly detection.

    Li, Chaobo / Li, Hongjun / Zhang, Guoan

    Neural networks : the official journal of the International Neural Network Society

    2024  Volume 172, Page(s) 106138

    Abstract: Video anomaly detection is an important task for public security in the multimedia field. It aims to distinguish events that deviate from normal patterns. As important semantic representation, the textual information can effectively perceive different ... ...

    Abstract Video anomaly detection is an important task for public security in the multimedia field. It aims to distinguish events that deviate from normal patterns. As important semantic representation, the textual information can effectively perceive different contents for anomaly detection. However, most existing methods primarily rely on visual modality, with limited incorporation of textual modality in anomaly detection. In this paper, a cross-modality integration framework (CIForAD) is proposed for anomaly detection, which combines both textual and visual modalities for prediction, perception and discrimination. Firstly, a feature fusion prediction (FUP) module is designed to predict the target regions by fusing the visual features and textual features for prompting, which can amplify the discriminative distance. Then an image-text semantic perception (ISP) module is developed to judge semantic consistency by associating the fine-grained visual features with textual features, where a strategy of local training and global inference is introduced to perceive local details and global semantic correlation. Finally, a self-supervised time attention discrimination (TAD) module is built to explore the inter-frame relation and further distinguish abnormal sequences from normal sequences. Extensive experiments on the three challenging benchmarks indicate that our CIForAD obtains state-of-the-art anomaly detection performance.
    MeSH term(s) Multimedia ; Benchmarking ; Semantics ; Perception
    Language English
    Publishing date 2024-01-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 740542-x
    ISSN 1879-2782 ; 0893-6080
    ISSN (online) 1879-2782
    ISSN 0893-6080
    DOI 10.1016/j.neunet.2024.106138
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Role of STAT3 in cancer cell epithelial‑mesenchymal transition (Review).

    Zhang, Guoan / Hou, Sen / Li, Shuyue / Wang, Yequan / Cui, Wen

    International journal of oncology

    2024  Volume 64, Issue 5

    Abstract: Since its discovery, the role of the transcription factor, signal transducer and activator of transcription 3 (STAT3), in both normal physiology and the pathology of numerous diseases, including cancer, has been extensively studied. STAT3 is aberrantly ... ...

    Abstract Since its discovery, the role of the transcription factor, signal transducer and activator of transcription 3 (STAT3), in both normal physiology and the pathology of numerous diseases, including cancer, has been extensively studied. STAT3 is aberrantly activated in different types of cancer, fulfilling a critical role in cancer progression. The biological process, epithelial‑mesenchymal transition (EMT), is indispensable for embryonic morphogenesis. During the development of cancer, EMT is hijacked to confer motility, tumor cell stemness, drug resistance and adaptation to changes in the microenvironment. The aim of the present review was to outline recent advances in knowledge of the role of STAT3 in EMT, which may contribute to the understanding of the function of STAT3 in EMT in various types of cancer. Delineating the underlying mechanisms associated with the STAT3‑EMT signaling axis may generate novel diagnostic and therapeutic options for cancer treatment.
    MeSH term(s) Humans ; STAT3 Transcription Factor/genetics ; STAT3 Transcription Factor/metabolism ; Epithelial-Mesenchymal Transition/physiology ; Signal Transduction/physiology ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Cell Movement ; Neoplasms/genetics
    Chemical Substances STAT3 Transcription Factor ; STAT3 protein, human
    Language English
    Publishing date 2024-03-15
    Publishing country Greece
    Document type Review ; Journal Article
    ZDB-ID 1154403-x
    ISSN 1791-2423 ; 1019-6439
    ISSN (online) 1791-2423
    ISSN 1019-6439
    DOI 10.3892/ijo.2024.5636
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Insignificant Difference in Early Post-injury Gene Expression Between Patients with Burns Only and Those with Inhalation Injury: A Bioinformatics Analysis.

    Jiang, Huihao / Dou, Zhe / Chen, Guangyu / Zhang, Guoan / Du, Weili

    Journal of burn care & research : official publication of the American Burn Association

    2023  Volume 44, Issue 6, Page(s) 1509–1518

    Abstract: Airway obstruction is fatal but common among burn patients in the early period after inhalation injury, during which most tracheotomies are performed within 48 h post-injury. Inflammation is common in laryngoscopy; however, the related gene expression ... ...

    Abstract Airway obstruction is fatal but common among burn patients in the early period after inhalation injury, during which most tracheotomies are performed within 48 h post-injury. Inflammation is common in laryngoscopy; however, the related gene expression has rarely been studied. In this study, we obtained the data of healthy control and patient samples collected within 8-48 hours post-injury from the Gene Expression Omnibus database and classified them into 10 inhalation-injury patients, 6 burn-only, and 10 healthy controls. Differential gene expression was identified between the patient groups; however, principal component analysis and cluster analysis indicated a similarity between groups. Furthermore, enrichment analysis, Kyoto Encyclopedia of Genes and Genomes, and gene set enrichment analyses showed no significant differences in immune regulation and cell adjustment between the patient groups; but differences were shown when comparing either patient group to the healthy control group, including prominent regulation in inflammatory cells, infection, and cell adjustment. Thus, the gene expression in inhalation injury and burn-only patients does not significantly differ in the early period after injury, especially in inflammation, indicating the absence of specific diagnostic markers or anti-inflammatory treatment in inhalation injury patients, with the potential to identify more subtle differences. Further research is warranted.
    MeSH term(s) Humans ; Burns ; Burns, Inhalation/surgery ; Laryngoscopy ; Inflammation ; Gene Expression
    Language English
    Publishing date 2023-06-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2224246-6
    ISSN 1559-0488 ; 1559-047X
    ISSN (online) 1559-0488
    ISSN 1559-047X
    DOI 10.1093/jbcr/irad085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Temperature processing and distribution in larynx thermal inhalation injury with analogy to human airway cells: a mechanism of protection.

    Jiang, Huihao / Zhou, Xiaocheng / Zhang, Guoan

    American journal of translational research

    2022  Volume 14, Issue 6, Page(s) 3796–3805

    Abstract: Objective: Inhalation injuries, especially laryngeal injuries, threaten the lives of burn patients. Unlike studies on temperature distribution in the upper airway, studies on temperature development in different laryngeal layers, including the mucosa, ... ...

    Abstract Objective: Inhalation injuries, especially laryngeal injuries, threaten the lives of burn patients. Unlike studies on temperature distribution in the upper airway, studies on temperature development in different laryngeal layers, including the mucosa, lamina propria, cartilage, muscle, and subcutaneous layer, are lacking.
    Method: For the
    Results: No statistical difference emerged between the temperatures of different laryngeal layers at each timepoint. The temperatures decreased significantly and shortly before increasing unevenly in the low- and medium-heat groups. The survival rates and viability of the two cell lines correlated negatively with heat energy. The heat energy absorbed in the low-, medium-, and high-heat groups of beagles were 12, 29, and 44 J/g, with calculated
    Conclusions: The abnormal temperature processing and lack of a difference between layers indicate an effective self-protective mechanism of heat conduction in larynx. The
    Language English
    Publishing date 2022-06-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2471058-1
    ISSN 1943-8141
    ISSN 1943-8141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons.

    Zhang, Guoan / Deinhardt, Katrin / Neubert, Thomas A

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2603, Page(s) 163–171

    Abstract: Cultured primary neurons are a well-established model for the study of neuronal function. Conventional stable isotope labeling with amino acids in cell culture (SILAC) requires nearly complete metabolic labeling of proteins and therefore is difficult to ... ...

    Abstract Cultured primary neurons are a well-established model for the study of neuronal function. Conventional stable isotope labeling with amino acids in cell culture (SILAC) requires nearly complete metabolic labeling of proteins and therefore is difficult to apply to cultured primary neurons, which do not divide in culture. In a multiplex SILAC strategy, two different sets of heavy amino acids are used for labeling cells for the different experimental conditions. This allows for straightforward SILAC quantitation using partially labeled cells because the two cell populations are always equally labeled. When combined with bioorthogonal noncanonical amino acid tagging (BONCAT), it allows for comparative proteomic analysis of de novo protein synthesis. Here we describe protocols that utilize the multiplex SILAC labeling strategy for primary cultured neurons to study steady-state and nascent proteomes.
    MeSH term(s) Isotope Labeling/methods ; Amino Acids/chemistry ; Proteomics/methods ; Proteome/metabolism ; Neurons/metabolism
    Chemical Substances Amino Acids ; Proteome
    Language English
    Publishing date 2022-11-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2863-8_13
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: [Lay emphasis on the treatment and exploration of extraordinary injuries].

    Zhang, Guoan

    Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns

    2015  Volume 31, Issue 6, Page(s) 401–403

    Abstract: Treatment of "extraordinary injuries" is a major challenge for surgeons major in burn care and plastic surgery, though they are experts in wound treatment and repair. The "extraordinary injuries" is very complicated, and its treatment needs ... ...

    Abstract Treatment of "extraordinary injuries" is a major challenge for surgeons major in burn care and plastic surgery, though they are experts in wound treatment and repair. The "extraordinary injuries" is very complicated, and its treatment needs multidisciplinary cooperation. Surgeons not only have to master the relevant knowledge, but also should have the expertise to choose the appropriate treatment targeting the special pathological characteristics of the extraordinary wounds. Therefore, surgeons should learn and aggregate more knowledge regarding extraordinary injuries, strengthen muture communication, and encourage research work.
    MeSH term(s) Burns/surgery ; Humans ; Patient Care Team ; Reconstructive Surgical Procedures/methods ; Surgery, Plastic ; Wound Healing
    Language Chinese
    Publishing date 2015-12
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 2229222-6
    ISSN 1009-2587
    ISSN 1009-2587
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: m6A-related bioinformatics analysis and functional characterization reveals that METTL3-mediated NPC1L1 mRNA hypermethylation facilitates progression of atherosclerosis via inactivation of the MAPK pathway.

    Zhang, Guoan / Li, Xuewen / Huang, Xiaoyan

    Inflammation research : official journal of the European Histamine Research Society ... [et al.

    2022  Volume 72, Issue 3, Page(s) 429–442

    Abstract: Objective: Accumulating evidence has demonstrated that N6-methyladenosine (m6A) plays important roles in many major diseases, including atherosclerosis (AS). In the present study, we aimed to explore the transcriptomic m6A landscape of endothelial ... ...

    Abstract Objective: Accumulating evidence has demonstrated that N6-methyladenosine (m6A) plays important roles in many major diseases, including atherosclerosis (AS). In the present study, we aimed to explore the transcriptomic m6A landscape of endothelial function-associated genes and identify potential regulators in AS progression.
    Methods: The GEO data (GSE142386) from MeRIP-seq in human umbilical vein endothelial cells (HUVECs) with METTL3 knocked down or not were analyzed. RNA-seq was performed to identify differences in gene expression. Gene ontology (GO) functional and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses were conducted to evaluate the potential functions of the differentially expressed genes. MeRIP-qPCR was used to measure the m6A and mRNA levels of the top 8 downregulated genes, and NPC1L1 was selected as the candidate gene. Oxidized low-density lipoprotein (ox-LDL) was used to stimulate HUVECs, and METTL3 or NPC1L1 was silenced in ox-LDL-treated cells. And Transwell, ELISA, and cell apoptosis assays were performed to assess cell functional injury. ApoE-/- mice were fed with high-fat diet for 8 weeks to establish an AS model, and adenovirus-mediated NPC1L1 shRNA or NC shRNA was injected into the mice through the tail vein. Mouse aortic tissue damage and plaque deposition were evaluated by H&E, Oil Red O, and TUNEL staining.
    Results: One hundred and ninety-four hypermethylated m6A peaks and 222 hypomethylated peaks were detected in response to knockdown of METTL3. Genes with altered m6A peaks were significantly involved in the histone modification, enzyme activity, and formation of multiple complexes and were predominantly enriched in the MAPK pathway. NPC1L1 was a most significantly downregulated transcript in response to knockdown of METTL3. Moreover, knockdown of NPC1L1 or de-m6A (METTL3 knockdown)-mediated downregulation of NPC1L1 could improve ox-LDL-induced dysfunction of HUVECs in vitro and high-fat diet-induced atherosclerotic plaque in vivo, which was associated with the inactivation of the MAPK pathway.
    Conclusion: METTL3-mediated NPC1L1 mRNA hypermethylation facilitates AS progression by regulating the MAPK pathway, and NPC1L1 may be a novel target for the treatment of AS.
    MeSH term(s) Animals ; Humans ; Male ; Mice ; Atherosclerosis/drug therapy ; Atherosclerosis/metabolism ; Human Umbilical Vein Endothelial Cells ; Methylation ; Methyltransferases/metabolism ; Membrane Transport Proteins/genetics ; RNA, Messenger/metabolism
    Chemical Substances Methyltransferases (EC 2.1.1.-) ; METTL3 protein, human (EC 2.1.1.62) ; NPC1L1 protein, human ; Membrane Transport Proteins ; RNA, Messenger
    Language English
    Publishing date 2022-12-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1221794-3
    ISSN 1420-908X ; 1023-3830
    ISSN (online) 1420-908X
    ISSN 1023-3830
    DOI 10.1007/s00011-022-01681-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: [Treatment should be individualized for burn injury in extraordinary regions or caused by uncommon agents].

    Zhang, Guoan

    Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns

    2014  Volume 30, Issue 5, Page(s) 392–393

    Abstract: Burn wounds in extraordinary regions or caused by uncommon agents need individualized treatment according to their individual particularity. A perusal of papers to be published in this issue showed that most of burn units have paid attention to burn ... ...

    Abstract Burn wounds in extraordinary regions or caused by uncommon agents need individualized treatment according to their individual particularity. A perusal of papers to be published in this issue showed that most of burn units have paid attention to burn injuries pertaining to this category in their regular performance, and individualized treatment has been advocated. Accordingly, the treatment result of burns of this category has been improved. Furthermore, aesthetic and functional recovery has also been emphasized, thus the quality of life of the victim has been improved evidently, and they are able to return to family and society early.
    MeSH term(s) Burn Units ; Burns/psychology ; Burns/surgery ; Humans ; Precision Medicine ; Quality Improvement ; Quality of Life ; Treatment Outcome
    Language Chinese
    Publishing date 2014-10
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 2229222-6
    ISSN 1009-2587
    ISSN 1009-2587
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Comprehensive analysis of immune cell landscapes revealed that immune cell ratio eosinophil/B.cell.memory is predictive of survival in sepsis.

    Wang, Lei / Zhang, Guoan / Sun, Wenjie / Zhang, Yan / Tian, Yi / Yang, Xiaohui / Liu, Yingfu

    European journal of medical research

    2023  Volume 28, Issue 1, Page(s) 565

    Abstract: Background: Immune dysregulation is a feature of sepsis. However, a comprehensive analysis of the immune landscapes in septic patients has not been conducted.: Objectives: This study aims to explore the abundance ratios of immune cells in sepsis and ... ...

    Abstract Background: Immune dysregulation is a feature of sepsis. However, a comprehensive analysis of the immune landscapes in septic patients has not been conducted.
    Objectives: This study aims to explore the abundance ratios of immune cells in sepsis and investigate their clinical value.
    Methods: Sepsis transcriptome data sets were downloaded from the NCBI GEO database. The immunedeconv R package was employed to analyze the abundance of immune cells in sepsis patients and calculate the ratios of different immune cell types. Differential analysis of immune cell ratios was performed using the t test. The Spearman rank correlation coefficient was utilized to find the relationships between immune cell abundance and pathways. The prognostic significance of immune cell ratios for patient survival probability was assessed using the log-rank test. In addition, differential gene expression was performed using the limma package, and gene co-expression analysis was executed using the WGCNA package.
    Results: We found significant changes in immune cell ratios between sepsis patients and healthy controls. Some of these ratios were associated with 28-day survival. Certain pathways showed significant correlations with immune cell ratios. Notably, six immune cell ratios demonstrated discriminative ability for patients with systemic inflammatory response syndrome (SIRS), bacterial sepsis, and viral sepsis, with an Area Under the Curve (AUC) larger than 0.84. Patients with a high eosinophil/B.cell.memory ratio exhibited poor survival outcomes. A total of 774 differential genes were identified in sepsis patients with a high eosinophil/B.cell.memory ratio compared to those with a low ratio. These genes were organized into seven co-expression modules associated with relevant pathways, including interferon signaling, T-cell receptor signaling, and specific granule pathways.
    Conclusions: Immune cell ratios eosinophil/B.cell.memory and NK.cell.activated/NK.cell.resting in sepsis patients can be utilized for disease subtyping, prognosis, and diagnosis. The proposed cell ratios may have higher prognostic values than the neutrophil-to-lymphocyte ratio (NLR).
    MeSH term(s) Humans ; Eosinophils ; ROC Curve ; Sepsis/genetics ; Sepsis/diagnosis ; Systemic Inflammatory Response Syndrome ; Prognosis ; Killer Cells, Natural ; Retrospective Studies
    Language English
    Publishing date 2023-12-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 1329381-3
    ISSN 2047-783X ; 0949-2321
    ISSN (online) 2047-783X
    ISSN 0949-2321
    DOI 10.1186/s40001-023-01506-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Gga3

    Zhang, Xiaohong / Yue, Song / Jiang, Dongjun / Zhang, Guoan / Liu, Jianli / Li, Xuezhi

    Genes & diseases

    2023  Volume 10, Issue 6, Page(s) 2206–2209

    Language English
    Publishing date 2023-03-27
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2821806-1
    ISSN 2352-3042 ; 2352-3042
    ISSN (online) 2352-3042
    ISSN 2352-3042
    DOI 10.1016/j.gendis.2023.02.031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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