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  1. Article ; Online: Decoding tumor microenvironments through artificial tumor transcriptomes.

    Tian, Liqing / Zhang, Jinghui

    Cancer cell

    2022  Volume 40, Issue 8, Page(s) 809–811

    Abstract: In this issue of Cancer Cell, Zaitsev et al. (2022) present a machine-learning-based approach, trained from millions of artificial transcriptomes with admixed cell populations, for reconstructing tumor microenvironments (TMEs). The high accuracy of this ... ...

    Abstract In this issue of Cancer Cell, Zaitsev et al. (2022) present a machine-learning-based approach, trained from millions of artificial transcriptomes with admixed cell populations, for reconstructing tumor microenvironments (TMEs). The high accuracy of this approach, demonstrated through extensive validation, enables systematic investigation of TMEs in both research and clinical settings.
    MeSH term(s) Humans ; Machine Learning ; Neoplasms/genetics ; Transcriptome ; Tumor Microenvironment/genetics
    Language English
    Publishing date 2022-08-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 2078448-X
    ISSN 1878-3686 ; 1535-6108
    ISSN (online) 1878-3686
    ISSN 1535-6108
    DOI 10.1016/j.ccell.2022.07.008
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  2. Article ; Online: Peptide Hormone Insulin Regulates Function, Expression, and SUMOylation of Organic Anion Transporter 3.

    Zhang, Jinghui / You, Guofeng

    The AAPS journal

    2021  Volume 23, Issue 2, Page(s) 41

    Abstract: Organic anion transporter 3 (OAT3) plays an important role in the disposition of various anionic drugs which impacts the pharmacokinetics and pharmacodynamics of the therapeutics, thus influencing the pharmacological effects and toxicity of the drugs. In ...

    Abstract Organic anion transporter 3 (OAT3) plays an important role in the disposition of various anionic drugs which impacts the pharmacokinetics and pharmacodynamics of the therapeutics, thus influencing the pharmacological effects and toxicity of the drugs. In this study, we investigated the effect of insulin on the regulation of OAT3 function, expression, and SUMOylation. We demonstrated that insulin induced an increase in OAT3 transport activity through a dose- and time-dependent manner in COS-7 cells. The insulin-induced elevation in OAT3 function was blocked by PKA inhibitor H89, which correlated well with OAT3 protein expression. Moreover, both PKA activator Bt2-cAMP-induced increase and insulin-induced increase in OAT3 function were blocked by PKB inhibitor AKTi1/2. To further investigate the involvement of SUMOylation, we treated OAT3-expressing cells with insulin in presence or absence of H89 or AKTi1/2 followed by examining OAT3 SUMOylation. We showed that insulin enhanced OAT3 SUMOylation, and such enhancement was abrogated by H89 and AKTi1/2. Lastly, insulin increased OAT3 function and SUMOylation in rat kidney slice. In conclusion, our investigations demonstrated that insulin regulated OAT3 function, expression, and SUMOylation through PKA/PKB signaling pathway. Graphical abstract.
    MeSH term(s) Animals ; COS Cells ; Chlorocebus aethiops ; Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Diabetes Mellitus, Type 2/drug therapy ; Estrone/analogs & derivatives ; Estrone/metabolism ; Humans ; Insulin, Regular, Human/pharmacology ; Insulin, Regular, Human/therapeutic use ; Isoquinolines ; Kidney/drug effects ; Kidney/metabolism ; Models, Animal ; Organic Anion Transporters, Sodium-Independent/metabolism ; Protein Kinase Inhibitors/pharmacology ; Proto-Oncogene Proteins c-akt/metabolism ; Rats ; Recombinant Proteins/pharmacology ; Recombinant Proteins/therapeutic use ; Signal Transduction/drug effects ; Sulfonamides ; Sumoylation/drug effects
    Chemical Substances Insulin, Regular, Human ; Isoquinolines ; Organic Anion Transporters, Sodium-Independent ; Protein Kinase Inhibitors ; Recombinant Proteins ; Sulfonamides ; organic anion transport protein 3 ; Estrone (2DI9HA706A) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Cyclic AMP-Dependent Protein Kinases (EC 2.7.11.11) ; N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide (M876330O56) ; estrone sulfate (QTL48N278K)
    Language English
    Publishing date 2021-03-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1550-7416
    ISSN (online) 1550-7416
    DOI 10.1208/s12248-021-00575-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A Study on Refraction Error Compensation Method for Underwater Spinning Laser Scanning Three-Dimensional Imaging.

    Zhang, Jinghui / Wang, Yuhang / Zhang, Tao / Yang, Kai / Zhang, Jian / Wang, Xinyu

    Sensors (Basel, Switzerland)

    2024  Volume 24, Issue 2

    Abstract: Laser scanning 3D imaging technology, because it can obtain accurate three-dimensional surface data, has been widely used in the search for wrecks and rescue operations, underwater resource development, and other fields. At present, the conventional ... ...

    Abstract Laser scanning 3D imaging technology, because it can obtain accurate three-dimensional surface data, has been widely used in the search for wrecks and rescue operations, underwater resource development, and other fields. At present, the conventional underwater spinning laser scanning imaging system maintains a relatively fixed light window. However, in low-light situations underwater, the rotation of the scanning device causes some degree of water fluctuation, which warps the light strip data that the system sensor receives about the object's surface. To solve this problem, this research studies an underwater 3D scanning and imaging system that makes use of a fixed light window and a spinning laser (FWLS). A refraction error compensation algorithm is investigated that is based on the fundamentals of linear laser scanning imaging, and a dynamic refraction mathematical model is established based on the motion of the imaging device. The results of the experiment on error analysis in an optimal underwater environment indicate that the error in reconstructing the radius is decreased by 60% (from 2.5 mm to around 1 mm) when compensating for the measurement data of a standard sphere with a radius of 20 mm. Moreover, the compensated point cloud data exhibit a higher degree of correspondence with the model of the standard spherical point cloud. Furthermore, we examine the impact of physical noise, measurement distance, and partial occlusion of the object on the imaging system inside an authentic underwater setting. This study is a good starting point for looking at the refractive error of an underwater laser scanning imaging system. It also provides to us some ideas for future research on the refractive error of other scanning imaging methods.
    Language English
    Publishing date 2024-01-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s24020343
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  4. Article: Intern-Nursing Students' Knowledge of Vascular Catheter-Associated Infections and Its Associated Factors: A Cross-Sectional Survey in China.

    Hu, Jiaji / Xu, Binbin / Yao, Nian / Peng, Sha / Xu, Ran / Ma, Guiyuan / Zhang, Jinghui

    Risk management and healthcare policy

    2024  Volume 17, Page(s) 423–433

    Abstract: Background: Medical personnel contact with the patient closely, and their knowledge of vascular catheter-associated infections (VCAIs) is closely related to the prevention of VCAIs. Researchers mainly pay attention to the VCAIs knowledge of doctors and ... ...

    Abstract Background: Medical personnel contact with the patient closely, and their knowledge of vascular catheter-associated infections (VCAIs) is closely related to the prevention of VCAIs. Researchers mainly pay attention to the VCAIs knowledge of doctors and nurses but rarely pay attention to the nursing students in the hospital internship stage.
    Purpose: To investigate the current situation of knowledge of intern-nursing students in VCAIs, and analyze its influencing factors.
    Patients and methods: 843 intern-nursing students were selected from 10 hospitals in five regions of eastern, western, southern, northern, and central China from June 26 to July 31, 2023, using a two-stage random sampling method. A self-designed questionnaire with good reliability and validity was used to investigate their knowledge of VCAIs, and
    Results: Intern nursing students' mean score of VCAIs knowledge was 48.66 (SD=15.77), with a score below 60 (unqualified) accounting for 75.4%, a score of 60-79 (qualified) accounting for 19.7%, a score of 80-89 (good) accounting for 3.6%, and a score of above 90 accounting for 1.3%. Students who attended VCAIs training three or more times had higher scores than those who did not attend training (B: 4.706, p=0.001), knowledge scores of students with a bachelor's degree or above were higher than those with junior college degree or below (B: 8.479, p<0.001), students who interned in tertiary hospitals had higher scores than those practicing in secondary hospitals (B:12.381, p<0.001) and scores of students in hospital training were significantly higher than study independently (B:4.116, p=0.007).
    Conclusion: Intern-nursing students have a relatively low level of knowledge about VCAIs. It is recommended to strengthen clinical systematic and standardized training, improve the knowledge mastery level of intern-nursing students, and enhance their ability to handle VCAIs.
    Language English
    Publishing date 2024-02-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2495128-6
    ISSN 1179-1594
    ISSN 1179-1594
    DOI 10.2147/RMHP.S455964
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Clinical Response to a PARP Inhibitor and Chemotherapy in a Child with BARD1-Mutated Refractory Neuroblastoma: A Case Report.

    Cupit-Link, Maggie / Hagiwara, Kohei / Zhang, Jinghui / Federico, Sara M

    Research square

    2023  

    Abstract: Despite advances in the treatment of high-risk neuroblastoma, approximately half of these patients die from the disease. Targeted therapy based on synthetic lethality associated with homologous recombination deficiency (HRD) caused by germline mutations ... ...

    Abstract Despite advances in the treatment of high-risk neuroblastoma, approximately half of these patients die from the disease. Targeted therapy based on synthetic lethality associated with homologous recombination deficiency (HRD) caused by germline mutations in homologous recombination repair genes has shown great efficacy in several adult solid tumors. Here we report the first successful treatment of a pediatric patient with refractory neuroblastoma and a germline pathogenic mutation in
    Language English
    Publishing date 2023-08-16
    Publishing country United States
    Document type Preprint
    DOI 10.21203/rs.3.rs-3250117/v1
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  6. Article ; Online: Metabolomic analysis reveals the metabolic disturbance in aortic dissection: Subtype difference and accurate diagnosis.

    Zhang, Jinghui / Han, Lu / Liu, Hongchuan / Zhang, Hongjia / An, Zhuoling

    Nutrition, metabolism, and cardiovascular diseases : NMCD

    2023  Volume 33, Issue 8, Page(s) 1556–1564

    Abstract: Background and aims: Aortic dissection (AD), a severe clinical emergency with high mortality, is easily misdiagnosed as are other cardiovascular diseases. This study aimed at discovering plasma metabolic markers with the potential to diagnose AD and ... ...

    Abstract Background and aims: Aortic dissection (AD), a severe clinical emergency with high mortality, is easily misdiagnosed as are other cardiovascular diseases. This study aimed at discovering plasma metabolic markers with the potential to diagnose AD and clarifying the metabolic differences between two subtypes of AD.
    Methods and results: To facilitate the diagnosis of AD, we investigated the plasma metabolic profile by metabolomic approach. A total 482 human subjects were enrolled in the study: 80 patients with AD (50 with Stanford type A and 30 with Stanford type B), 198 coronary artery disease (CAD) patients, and 204 healthy individuals. Plasma samples were submitted to targeted metabolomic analysis. The partial least-squares discriminant analysis models were constructed to illustrate clear discrimination of AD patients with CAD patients and healthy control. Subsequently, the metabolites that were clinically relevant to the disturbances in AD were identified. Twenty metabolites induced the separation of AD patients and healthy control, 9 of which caused the separation of CAD patients and healthy control. There are 11 metabolites specifically down-regulated in AD group. Subgroup analysis showed that the levels of glycerol and uridine were dramatically lower in the plasma of patients with Stanford type A AD than those in the healthy control or Stanford type B AD groups.
    Conclusion: This study characterized metabolomic profiles specifically associated with the pathogenesis and development of AD. The findings of this research may potentially lead to earlier diagnosis and treatment of AD.
    MeSH term(s) Humans ; Aortic Dissection/diagnosis ; Metabolomics/methods ; Metabolome ; Coronary Artery Disease/diagnosis
    Language English
    Publishing date 2023-05-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1067704-5
    ISSN 1590-3729 ; 0939-4753
    ISSN (online) 1590-3729
    ISSN 0939-4753
    DOI 10.1016/j.numecd.2023.05.006
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    Zs.A 3149: Show issues Location:
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  7. Article ; Online: Water-use strategies and functional traits explain divergent linkages in physiological responses to simulated precipitation change.

    Wang, Yang / Zhang, Cicheng / Xiao, Xiong / Wu, Huawu / Zhang, Jinghui

    The Science of the total environment

    2023  Volume 908, Page(s) 168238

    Abstract: As a part of global climate change, precipitation patterns in arid regions will change significantly, and the different responses of desert plants to these changes will lead to alterations in community composition, thereby impacting ecosystem stability. ... ...

    Abstract As a part of global climate change, precipitation patterns in arid regions will change significantly, and the different responses of desert plants to these changes will lead to alterations in community composition, thereby impacting ecosystem stability. Thus, understanding the mechanism underlying the associations among physiological response variables considering changing precipitation is crucial. Here, water-use strategies, functional traits, and physiological processes (e.g., photosynthesis (A
    MeSH term(s) Water ; Ecosystem ; Plant Leaves ; Desert Climate ; Plants ; Droughts ; Soil
    Chemical Substances Water (059QF0KO0R) ; Soil
    Language English
    Publishing date 2023-11-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2023.168238
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  8. Article ; Online: Bidirectional association between visual impairment and frailty among community-dwelling older adults: a longitudinal study.

    Hou, Tianxue / Liu, Minhui / Zhang, Jinghui

    BMC geriatrics

    2022  Volume 22, Issue 1, Page(s) 672

    Abstract: Background: Vision impairment is common among older adults, and it may be related to frailty. However, the longitudinal relationship between visual impairment and frailty is still unclear.: Methods: We used data from Round 1 to Round 5 from the ... ...

    Abstract Background: Vision impairment is common among older adults, and it may be related to frailty. However, the longitudinal relationship between visual impairment and frailty is still unclear.
    Methods: We used data from Round 1 to Round 5 from the National Health and Aging Trend Study. Two samples were community-dwelling older adults, sample 1 (without visual impairment) was classified according to whether they have pre-frailty/frailty at R1 (N = 3013) and sample 2 (without pre-frailty/frailty) was classified according to whether they have visual impairment at R1 (N = 1422), respectively. Frailty was measured using five criteria: experiencing exhaustion, unintentional weight loss, low physical activity, slow gait, and weak grip strength. Visual impairment was assessed by asking participants if they had any visual impairment. Generalized estimating equation models were used to examine the concurrent and lagged association between visual impairment and frailty.
    Results: The participants were on average 76 ± 7 years old, female (59%), non-Hispanic white (74%) with less than bachelor educated (73%), and 44% were pre-frail/frail in the older adults without visual impairment. Approximately 5% of participants had visual impairment at R1, and they tended to be female and non-Hispanic White in the older adults without frailty. The concurrent (OR, 95% CI = 1.55, 1.17-2.02) and lagged (OR, 95% CI = 1.79, 1.25-2.59) associations between frailty and visual impairment were significantly after controlling the covariates. Similarly, the concurrent (OR, 95% CI = 1.63, 1.32-2.04) and lagged (OR, 95% CI = 1.49, 1.20-1.87) associations between visual impairment and frailty were also significant.
    Conclusions: Overall, this study provides evidence for a longitudinal, bidirectional association between self-reported visual impairment and frailty. Future intervention programs to delay frailty progression should include strategies that may reduce the incidence of visual impairment.
    MeSH term(s) Aged ; Aged, 80 and over ; Female ; Frail Elderly ; Frailty/diagnosis ; Frailty/epidemiology ; Geriatric Assessment ; Humans ; Independent Living ; Longitudinal Studies ; Vision, Low/diagnosis ; Vision, Low/epidemiology
    Language English
    Publishing date 2022-08-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2059865-8
    ISSN 1471-2318 ; 1471-2318
    ISSN (online) 1471-2318
    ISSN 1471-2318
    DOI 10.1186/s12877-022-03365-0
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  9. Article ; Online: Nuclear translocation of GluA2/ GAPDH promotes neurotoxicity after pilocarpine-induced epilepsy.

    Zhang, Jinghui / Qiao, Nana / Wang, Jiwen / Li, Baomin

    Epilepsy research

    2022  Volume 183, Page(s) 106945

    Abstract: Recent studies have identified that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) exerts multiple functions besides its role in energy metabolism. It can form a protein complex with GluA2 subunit of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ...

    Abstract Recent studies have identified that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) exerts multiple functions besides its role in energy metabolism. It can form a protein complex with GluA2 subunit of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), translocate into nucleus and confer neurotoxicity in a cerebral ischemia model. We postulate that GAPDH may also induce neurotoxicity by forming a coupling with GluA2 in pilocarpine-induced epileptic model, and disruption of the GluA2/GAPDH coupling can protect against neuronal injury. In this study, induced status epilepticus (SE) in rats by the systemic administration of pilocarpine, collected hippocampal tissues at different time points after SE, and assessed the relationship between GluA2/GAPDH coupling and neuronal apoptosis in SE rats. Then, we interrupted the GluA2/GAPDH coupling by a special interfering peptide and determined whether neuronal injury can be rescued and hippocampus-depended memory function can be improved. We also evaluated the concentrations of GAPDH in nuclear and cytoplasmatic proteins in SE group, non-SE group and after interruption of GluA2/GAPDH coupling, to verify the nuclear translocation of GAPDH in SE model. We found that the apoptosis of hippocampal neurons was most significant at 72 h after SE, which was also the peak time of GluA2/GAPDH coupling expression and GluA2 consumption. After interruption of GluA2/GAPDH coupling, the apoptosis and memorial function of hippocampal neurons were improved and nuclear translocation of GluA2/GAPDH coupling was reduced. In conclusion, GAPDH can be translocated into nucleus in the form of GluA2/GAPDH, which plays an important role in regulating pilocarpine-induced epilepsy via neurotoxicity pathway.
    MeSH term(s) Animals ; Disease Models, Animal ; Epilepsy/chemically induced ; Epilepsy/metabolism ; Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism ; Hippocampus/metabolism ; Pilocarpine/toxicity ; Rats ; Receptors, AMPA/metabolism ; Status Epilepticus/chemically induced ; Status Epilepticus/metabolism
    Chemical Substances Receptors, AMPA ; Pilocarpine (01MI4Q9DI3) ; Glyceraldehyde-3-Phosphate Dehydrogenases (EC 1.2.1.-) ; glutamate receptor ionotropic, AMPA 2 (P6W5IXV8V9)
    Language English
    Publishing date 2022-05-20
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632939-1
    ISSN 1872-6844 ; 0920-1211
    ISSN (online) 1872-6844
    ISSN 0920-1211
    DOI 10.1016/j.eplepsyres.2022.106945
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    Zs.A 2193: Show issues Location:
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  10. Article ; Online: Synergistic regulation of microglia differentiation by CD93 and integrin β1 in the rat pneumococcal meningitis model.

    Qiao, Nana / Zhang, Jinghui / Zhang, Ya / Liu, Xinjie

    Immunology letters

    2022  Volume 251-252, Page(s) 63–74

    Abstract: Streptococcus pneumoniae is the main bacterial pathogen of meningitis worldwide, which has a high mortality rate and survivors are prone to central nervous system (CNS) sequelae. In this regard, microglia activation has been associated with injury to the ...

    Abstract Streptococcus pneumoniae is the main bacterial pathogen of meningitis worldwide, which has a high mortality rate and survivors are prone to central nervous system (CNS) sequelae. In this regard, microglia activation has been associated with injury to the CNS. The aim of this study was to investigate the relationship between CD93, integrin β1, and microglia activation. In the rat pneumococcal meningitis model, we found significant increases of CD93 and integrin β1 expression and differentiation of M1 phenotype microglia. Furthermore, we showed in vitro siRNA-mediated downregulation of CD93 and integrin β1 expression after infecting highly aggressive proliferating immortalized (HAPI) microglia cells with S. pneumoniae. We observed differentiation of S. pneumonia-infected HAPI microglia cells to the M1 phenotype and significant release of soluble CD93 (sCD93) and integrin β1 expression. Complement C1q and metalloproteinases promoted sCD93 release. We also showed that downregulation of CD93 significantly reduced differentiation to M1 microglia and increased differentiation to M2 microglia. However, addition of recombinant CD93 may regulate microglia differentiation to the M1 phenotype. Furthermore, the downregulation of integrin β1 resulted in downregulation of the CD93 protein. In conclusion, interaction between integrin β1 and CD93 promotes differentiation of microglia to the M1 phenotype, increases the release of pro-inflammatory factors, and leads to nervous system injury in pneumococcal meningitis.
    Language English
    Publishing date 2022-11-04
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 445150-8
    ISSN 1879-0542 ; 0165-2478
    ISSN (online) 1879-0542
    ISSN 0165-2478
    DOI 10.1016/j.imlet.2022.11.001
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