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Article ; Online: Geographical region traceability of Poria cocos and correlation between environmental factors and biomarkers based on a metabolomic approach

Liu, Xiaoliu / Zhong, Can / Xie, Jing / Liu, Hao / Xie, Zhenni / Zhang, Shuihan / Jin, Jian

Food Chemistry. 2023 Aug., v. 417 p.135817-

2023

Abstract: The edible values of P. cocos from different origins vary significantly, therefore, it is important to investigate the traceability of geographical regions and identify the geographical biomarkers of P. cocos. The metabolites of P. cocos of the different ...

Abstract	The edible values of P. cocos from different origins vary significantly, therefore, it is important to investigate the traceability of geographical regions and identify the geographical biomarkers of P. cocos. The metabolites of P. cocos of the different geographical origins were assessed using liquid chromatography tandem-mass spectrometry, principal component analysis and orthogonal partial least-squares discriminant analysis (OPLS-DA). The OPLS-DA could clearly discriminate the metabolites of P. cocos from the three cultivation regions (YN, Yunnan; AH, Anhui; JZ, Hunan). Finally, three carbohydrates, four amino acids, and four triterpenoids were selected as biomarkers for P. cocos origin tracing. Correlation matrix analysis revealed that the contents of biomarkers were closely related to geographical origin. Altitude, temperature, and soil fertility were the main factors responsible for the differences in biomarker profiles in P. cocos. The metabolomics approach provides an effective strategy for tracing and identifying the biomarkers of P. cocos from different geographical origins.
Keywords	Poria ; altitude ; biomarkers ; discriminant analysis ; food chemistry ; least squares ; liquid chromatography ; metabolites ; metabolomics ; principal component analysis ; provenance ; soil fertility ; tandem mass spectrometry ; temperature ; traceability ; triterpenoids ; China ; YN ; AH ; JZ ; DTS ; UHPLC–MS/MS ; GC–MS/MS ; TIC ; PCA ; HCA ; OPLS-DA ; FC ; VIP ; EL ; Tmin ; Tmax ; DT ; RH ; API ; MSTR ; Poria cocos ; Geographical origin ; Untargeted metabolomics ; Environmental factors
Language	English
Dates of publication	2023-08
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2023.135817
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Identification of squalene epoxidase in triterpenes biosynthesis in Poria cocos by molecular docking and CRISPR-Cas9 gene editing.

Liu, Xiao-Liu / Xie, Jing / Xie, Zhen-Ni / Zhong, Can / Liu, Hao / Zhang, Shui-Han / Jin, Jian

Microbial cell factories

2024 Volume 23, Issue 1, Page(s) 34

Abstract: Background: Squalene epoxidase is one of the rate-limiting enzymes in the biosynthetic pathway of membrane sterols and triterpenoids. The enzyme catalyzes the formation of oxidized squalene, which is a common precursor of sterols and triterpenoids.: ... ...

Abstract	Background: Squalene epoxidase is one of the rate-limiting enzymes in the biosynthetic pathway of membrane sterols and triterpenoids. The enzyme catalyzes the formation of oxidized squalene, which is a common precursor of sterols and triterpenoids. Result: In this study, the squalene epoxidase gene (PcSE) was evaluated in Poria cocos. Molecular docking between PcSE and squalene was performed and the active amino acids were identified. The sgRNA were designed based on the active site residues. The effect on triterpene synthesis in P. cocos was consistent with the results from ultra-high-performance liquid chromatography-quadruplex time-of-flight-double mass spectrometry (UHPLC-QTOF-MS/MS) analysis. The results showed that deletion of PcSE inhibited triterpene synthesis. In vivo verification of PcSE function was performed using a PEG-mediated protoplast transformation approach. Conclusion: The findings from this study provide a foundation for further studies on heterologous biosynthesis of P. cocos secondary metabolites.
MeSH term(s)	Tandem Mass Spectrometry/methods ; Squalene Monooxygenase/genetics ; Squalene Monooxygenase/metabolism ; Wolfiporia/genetics ; Wolfiporia/metabolism ; Molecular Docking Simulation ; Squalene ; CRISPR-Cas Systems ; Gene Editing ; RNA, Guide, CRISPR-Cas Systems ; Triterpenes/metabolism ; Phytosterols
Chemical Substances	Squalene Monooxygenase (EC 1.14.14.17) ; Squalene (7QWM220FJH) ; RNA, Guide, CRISPR-Cas Systems ; Triterpenes ; Phytosterols
Language	English
Publishing date	2024-01-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2091377-1
ISSN	1475-2859 ; 1475-2859
ISSN (online)	1475-2859
ISSN	1475-2859
DOI	10.1186/s12934-024-02306-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: In Vitro

Xie, Guozhen / Zhou, Rongrong / Huang, Lili / Zhang, Shuihan / Tan, Zhoujin

Frontiers in chemistry

2022 Volume 10, Page(s) 907886

Abstract: The gut microbiota (GM) is involved in the metabolism of glycosides and is beneficial for enhancing their bioactivity. However, the metabolism of multi-glycosides by the GM under normal and pathological conditions is unclear. In this study, the total ... ...

Abstract	The gut microbiota (GM) is involved in the metabolism of glycosides and is beneficial for enhancing their bioactivity. However, the metabolism of multi-glycosides by the GM under normal and pathological conditions is unclear. In this study, the total glycosides (TG) of the traditional Chinese medicine (TCM) formula Qiwei Baizhu Powder (QWBZP) were extracted to represent a multi-glycoside system. Ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to rapidly identify the components and
Language	English
Publishing date	2022-06-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2711776-5
ISSN	2296-2646
ISSN	2296-2646
DOI	10.3389/fchem.2022.907886
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Beyond Alkaloids: Novel Bioactive Natural Products From

Zheng, Qinfang / Wang, Ye / Zhang, Shuihan

Frontiers in pharmacology

2021 Volume 12, Page(s) 638210

Abstract: In this work, we reviewed the progress in the phytochemical and biological investigations of bioactive components derived from medicinally ... ...

Abstract	In this work, we reviewed the progress in the phytochemical and biological investigations of bioactive components derived from medicinally valuable
Language	English
Publishing date	2021-03-08
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.638210
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Geographical region traceability of Poria cocos and correlation between environmental factors and biomarkers based on a metabolomic approach.

Liu, Xiaoliu / Zhong, Can / Xie, Jing / Liu, Hao / Xie, Zhenni / Zhang, Shuihan / Jin, Jian

Food chemistry

2023 Volume 417, Page(s) 135817

Abstract	The edible values of P. cocos from different origins vary significantly, therefore, it is important to investigate the traceability of geographical regions and identify the geographical biomarkers of P. cocos. The metabolites of P. cocos of the different geographical origins were assessed using liquid chromatography tandem-mass spectrometry, principal component analysis and orthogonal partial least-squares discriminant analysis (OPLS-DA). The OPLS-DA could clearly discriminate the metabolites of P. cocos from the three cultivation regions (YN, Yunnan; AH, Anhui; JZ, Hunan). Finally, three carbohydrates, four amino acids, and four triterpenoids were selected as biomarkers for P. cocos origin tracing. Correlation matrix analysis revealed that the contents of biomarkers were closely related to geographical origin. Altitude, temperature, and soil fertility were the main factors responsible for the differences in biomarker profiles in P. cocos. The metabolomics approach provides an effective strategy for tracing and identifying the biomarkers of P. cocos from different geographical origins.
MeSH term(s)	Chromatography, High Pressure Liquid/methods ; Wolfiporia/chemistry ; China ; Chromatography, Liquid ; Biomarkers ; Metabolomics/methods
Chemical Substances	Biomarkers
Language	English
Publishing date	2023-03-06
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2023.135817
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Protective effects of Fufang Ejiao Jiang against aplastic anemia assessed by network pharmacology and metabolomics strategy

He Dan / Zhang Haichao / Yi Ziyang / Zhao Di / Zhang Shuihan

Digital Chinese Medicine, Vol 4, Iss 4, Pp 328-

2021 Volume 342

Abstract: Objective: To elucidate the mechanisms underlying the therapeutic effects of Fufang Ejiao Jiang (复方阿胶浆, FFEJJ) on aplastic anemia (AA) using integrated network pharmacology and serum metabolomics. Methods: Traditional Chinese Medicine Systems ... ...

Abstract	Objective: To elucidate the mechanisms underlying the therapeutic effects of Fufang Ejiao Jiang (复方阿胶浆, FFEJJ) on aplastic anemia (AA) using integrated network pharmacology and serum metabolomics. Methods: Traditional Chinese Medicine Systems Pharmacology (TCMSP), Pubmed, integrative pharmacology-based research platform of traditional Chinese medicine (TCMIP), and Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine (BATMAN-TCM) were used to identify the constituents and putative targets of FFEJJ. GeneCards and DisGeNET databases were used to identify AA-associated targets. We constructed a herb-component-target network and analyzed the protein-protein interaction (PPI) network. Potential mechanisms were determined using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. In addition, an AA model was established using acetylphenylhydrazine (APH) and cetylphenylhydrazine (CTX). Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based serum metabolomics was applied to screen potential metabolites and the related pathways associated with AA and the potential anti-anemic effects of FFEJJ. Results: A total of 30 active components of FFEJJ and 24 targets were related to AA. PPI network analysis showed that VEGFA, AKT1, IL-6, CASP3, and ICAM1 were key nodes overlapping with proteins known to be related to AA. KEGG pathway enrichment analysis revealed that the presumed targets of FFEJJ were mainly associated with pathways linked to the promotion of hematopoiesis and improvement of the hematopoietic microenvironment. A total of 423 metabolite biomarkers were identified between the control and AA models, which are involved in the development of AA. In contrast, FFEJJ reversed the 79 differential metabolites altered by AA. Pathway analysis suggested that the synergistic effects of FFEJJ were mainly enriched in 24 metabolic pathways. Among them, sphingolipid metabolism, glycerophospholipid metabolism, and arachidonic ...
Keywords	Fufang Ejiao Jiang (复方阿胶浆 ; FFEJJ) ; aplastic anemia ; network pharmacology ; metabolomics ; lipid metabolomics ; Medicine ; R ; Other systems of medicine ; RZ201-999
Subject code	570
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	KeAi Communications Co., Ltd.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: A dual-emission carbon dots-based nonenzymatic fluorescent sensing platform for simultaneous detection of parathion-methyl and glyphosate

Yang, Yangyu / Tong, Xia / Chen, Yuxia / Zhou, Rongrong / Cai, Guihan / Wang, Tongtao / Zhang, Shuihan / Shi, Shuyun / Guo, Ying

Food chemistry. 2023 Mar. 01, v. 403

2023

Abstract: Simultaneous and high-performance detection of pesticides is still a considerable challenge and urgent need. Herein, a dual-emission carbon dots (CDs)-based nonenzymatic fluorescent sensing platform has been developed, which shows excellent sensitivity ... ...

Abstract	Simultaneous and high-performance detection of pesticides is still a considerable challenge and urgent need. Herein, a dual-emission carbon dots (CDs)-based nonenzymatic fluorescent sensing platform has been developed, which shows excellent sensitivity and selectivity in simultaneously detecting parathion-methyl (MP) and glyphosate. CDs with emissions at 440 nm (bCDs) and 660 nm (rCDs) were prepared by hydrothermal treatment of mulberry leaves and sodium hydroxide. bCDs response to hydrolyzed MP via inner filter effect, while rCDs sense glyphosate with the aid of Cu²⁺ by static quenching effect. Excellent linear correlations were found for MP (0.3–65.0 μM) and glyphosate (1.0–110.0 μM) with limits of detection at 0.14 and 0.60 μM. Notably, the presented dual-channel strategy was successfully applied in simultaneously detecting MP and glyphosate in food/herbal samples with acceptable recoveries, good precision, and high selectivity. Moreover, an ORlogicgatewas achieved for estimating food, herbal, or environmental safety.
Keywords	carbon ; fluorescence ; food chemistry ; glyphosate ; hot water treatment ; hydrolysis ; mulberries ; parathion-methyl ; sodium hydroxide
Language	English
Dates of publication	2023-0301
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.134346
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Total glycosides contribute to the anti-diarrheal effects of Qiwei Baizhu Powder

Xie, Guozhen / Deng, Na / Zheng, Tao / Peng, Xinxin / Zhang, Shuihan / Tan, Zhoujin

Frontiers in cellular and infection microbiology

2022 Volume 12, Page(s) 945263

Abstract: Qiwei Baizhu Powder (QWBZP) is a traditional Chinese medicine formula for treating diarrhea induced by various causes. It elicits an anti-diarrheal effect by regulating the gut microbiota (diversity, structure, and abundance). However, the contribution ... ...

Abstract	Qiwei Baizhu Powder (QWBZP) is a traditional Chinese medicine formula for treating diarrhea induced by various causes. It elicits an anti-diarrheal effect by regulating the gut microbiota (diversity, structure, and abundance). However, the contribution of different components in the QWBZP decoction to this effect remains unclear. In this study, we used the QWBZP decoction as a reference standard to investigate the effects of total glycosides (TGs) extracted from QWBZP decoction on the gut microbiota and bile acid metabolism in mice with antibiotic-associated diarrhea (AAD). The results of 16S rRNA gene sequencing and liquid chromatography-mass spectrometry (LC-MS) analysis showed that the effect of total glycosides of Qiwei Baizhu Powder (QWBZP-TG) on specific intestinal bacteria and bile acids was similar to that of the QWBZP decoction, but the intensity of this effect was more significant in the case of QWBZP-TG. The QWBZP decoction and QWBZP-TG promoted the proliferation of
MeSH term(s)	Animals ; Bile Acids and Salts/pharmacology ; Diarrhea/drug therapy ; Gastrointestinal Microbiome ; Glycosides ; Mice ; Powders/pharmacology ; RNA, Ribosomal, 16S/genetics
Chemical Substances	Bile Acids and Salts ; Glycosides ; Powders ; RNA, Ribosomal, 16S
Language	English
Publishing date	2022-08-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2022.945263
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: LC-MS Metabolite Profiling and the Hypoglycemic Activity of

Zhou, Qing Yi-Jun / Liao, Xin / Kuang, Hao-Ming / Li, Jia-Yu / Zhang, Shui-Han

Molecules (Basel, Switzerland)

2022 Volume 27, Issue 17

Abstract: ... Morus ... ...

Abstract	Morus alba
MeSH term(s)	Animals ; Blood Glucose/analysis ; Chromatography, Liquid ; Diabetes Mellitus, Type 2/drug therapy ; Hypoglycemic Agents/therapeutic use ; Mice ; Morus/chemistry ; Plant Extracts/chemistry ; Plant Leaves/chemistry ; Tandem Mass Spectrometry
Chemical Substances	Blood Glucose ; Hypoglycemic Agents ; Plant Extracts
Language	English
Publishing date	2022-08-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules27175360
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Article ; Online: Hinge-like paper-based dual-channel enzyme-free ratiometric fluorescent microfluidic platform for simultaneous visual detection of carbaryl and glyphosate.

Yang, Yangyu / Tong, Chaoying / Zhou, Rongrong / Qin, Ziyi / Xu, Jinju / Liao, Chunhui / Zhang, Shuihan / Shi, Shuyun / Guo, Ying

Food chemistry

2023 Volume 431, Page(s) 137127

Abstract: On-site multi-pesticide residues detection is particularly urgent and challenging. Here, we fabricated an enzyme-free ratiometric fluorescent detection system in combination with a hinge-like dual-channel 3D microfluidic paper analytical device (3D μPAD) ...

Abstract	On-site multi-pesticide residues detection is particularly urgent and challenging. Here, we fabricated an enzyme-free ratiometric fluorescent detection system in combination with a hinge-like dual-channel 3D microfluidic paper analytical device (3D μPAD) for simultaneous visual detection of carbaryl and glyphosate. Blue-emission 1-naphthol (Em. 470 nm) was hydrolyzed from carbaryl, while yellow-emission 2,3-diaminophenazine (Em. 570 nm) was produced with the aid of Cu
MeSH term(s)	Carbaryl ; Fluorescent Dyes/chemistry ; Microfluidics ; Quantum Dots/chemistry ; Carbon/chemistry ; Limit of Detection ; Glyphosate
Chemical Substances	Carbaryl (R890C8J3N1) ; 1-naphthol (2A71EAQ389) ; Fluorescent Dyes ; Carbon (7440-44-0)
Language	English
Publishing date	2023-08-11
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2023.137127
Database	MEDical Literature Analysis and Retrieval System OnLINE

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