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  1. Article ; Online: Effect of Hesperetin on Isoprenaline-induced Hypertrophy of H9C2 Cardiomyocytes.

    Liu, Bo / Qiu, Beilei / Zhang, Suchuan

    Cellular and molecular biology (Noisy-le-Grand, France)

    2022  Volume 68, Issue 7, Page(s) 85–89

    Abstract: An experiment was carried out to investigate the effect of hesperetin on isoproterenol-induced H9C2 cardiomyocyte hypertrophy and its possible mechanism. For this aim, H9C2 cardiomyocytes were coincubated with different concentrations of hesperetin (0 ... ...

    Abstract An experiment was carried out to investigate the effect of hesperetin on isoproterenol-induced H9C2 cardiomyocyte hypertrophy and its possible mechanism. For this aim, H9C2 cardiomyocytes were coincubated with different concentrations of hesperetin (0.125, 0.25, 0.5, 1μmol/L) and isoproterenol to detect the changes in the area of H9C2 cells, the expression of cardiac hypertrophy marker β-MHC mRNA and autophagy marker LC3II; H9C2 cells were coincubated with hesperetin and isoproterenol at the optimal intervention concentration, and the intervention time was set to 6h, 12h, 24h, respectively, and the changes of H9C2 cell area, β-MHC mRNA and LC3II expression were detected. Results showed that hesperetin could reduce isoproterenol-induced H9C2 cardiomyocyte area enlargement; hesperetin can reduce β-MHC mRNA expression in isoproterenol-induced H9C2 cardiomyocyte; hesperetin can increase the expression level of LC3II in isoproterenol-induced H9C2 cardiomyocyte. Conclusion Hesperetin may improve the hypertrophy of H9C2 cardiomyocytes induced by isoproterenol by activating autophagy.
    Language English
    Publishing date 2022-07-31
    Publishing country France
    Document type Journal Article
    ZDB-ID 1161779-2
    ISSN 1165-158X ; 0145-5680
    ISSN (online) 1165-158X
    ISSN 0145-5680
    DOI 10.14715/cmb/2022.68.7.14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Angelica polysaccharide promotes the proliferation of rat cardiomyocyte line H9c2

    YANG Ping, DAI Tian, ZHANG Su-chuan

    Jichu yixue yu linchuang, Vol 41, Iss 4, Pp 551-

    2021  Volume 557

    Abstract: Objective To study the effect of angelica polysaccharide on proliferation and apoptosis of rat cardiomyocyte line H9c2. Methods Rat cardiomyocyte H9c2 was divided into a control group (Control group), doxorubicin (ADR) induced H9c2 cell replication ... ...

    Abstract Objective To study the effect of angelica polysaccharide on proliferation and apoptosis of rat cardiomyocyte line H9c2. Methods Rat cardiomyocyte H9c2 was divided into a control group (Control group), doxorubicin (ADR) induced H9c2 cell replication dilated cardiomyopathy cardiomyocyte group (ADR group), APS intervention (APS group), transfection anti-miR-NC (APS+anti-miR-NC group) and transfected anti-miR-4701-3p(APS+anti-miR-4701-3p group). Flow cytometry was applied to determine apoptosis, Western blot was employed to test B cell lymphoma/leukemia-2(Bcl-2), Bcl-2 related X protein (Bax), nuclear related antigen Ki-67(Ki-67) and proliferation cell nuclear antigen (PCNA) protein expression, enzyme-linked immunoassay (ELISA) method was used to detect B-type brain natriuretic peptide (BNP) level. Cell proliferation was detected by commercially available kit 8(CCK-8), and real-time quantitative PCR (RT-qPCR) was used to analyze the microRNA-4701-3p(miR-4701-3p) expression. Results Adriamycin significantly increased BNP content, apoptosis rate, and Bax protein level in H9c2 cells, and greatly reduced Bcl-2 protein expression(P＜0.05). Angelica polysac-charide obviously increased H9c2 cell viability, Ki-67, PCNA, Bcl-2 protein expression and miR-4701-3p expression after doxorubicin induction, and remarkably reduced cell apoptosis and Bax protein expression(P＜0.05). Knockdown of miR-4701-3p significantly reduced the cell viability, Ki-67, PCNA, and Bcl-2 protein levels of dilated cardiomyopathy H9c2 cells treated with angelica polysaccharide, while significantly increased its apoptosis rate and Bax protein expression(P＜ 0.05). Conclusions Angelica polysaccharide up-regulates the expression of miR-4701-3p, promotes the proliferation of adriamycin-induced rat cardiomyocyte line H9c2.
    Keywords adriamycin|cell line h9c2|mir-4701-3p|bnp ; Medicine ; R
    Language Chinese
    Publishing date 2021-04-01T00:00:00Z
    Publisher Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Role of runt-related transcription factor 3 in myocardial fibrosis in mice

    ZHAO Qian / ZOU Yong / LIU Bo / YU Xinqiang / ZHANG Suchuan

    Di-san junyi daxue xuebao, Vol 43, Iss 24, Pp 2640-

    2021  Volume 2647

    Abstract: Objective To investigate the role of runt related transcription factor 3 (Runx3) in cardiac fibroblasts differentiation, proliferation as well as synthesis of type I collagen after myocardial infarction (MI) in mice. Methods Male C57BL/6J mice were ... ...

    Abstract Objective To investigate the role of runt related transcription factor 3 (Runx3) in cardiac fibroblasts differentiation, proliferation as well as synthesis of type I collagen after myocardial infarction (MI) in mice. Methods Male C57BL/6J mice were selected and randomly divided into model group (MI group, treated with ligation of left coronary artery) and Sham group, and were sacrificed at the 7th, 14th and 28th days after modeling (n=6 for each group), respectively. In addition, Runx3 small interfering RNA (si-Runx3) was injected via the tail vein of mice before modeling to achieve Runx3 knockdown, negative siRNA (si-NC) as the matched control. According to the treatment they received, the mice were subsequently allocated into Sham+si-NC group, Sham+si-Runx3 group, MI 28 d+si-NC group and MI 28 d+si-Runx3 group (n=8), and were sacrificed in 28 d after modeling. The levels of Runx3, alpha-smooth muscle actin (α-SMA) and type I collagen in the heart were determined by immunofluorescence staining and Western blotting. The cardiac function was evaluated by echocardiography, and Ki67 positive cells were observed using immunohistochemical assay. After cardiac fibroblasts were isolated from C57BL/6 mice and cultured for 1~3 d, and the effects of TGF-β1 at different concentrations and for different stimulation times on Runx3 protein were observed in the cells. The effects of Runx3 gene knockout on type I collagen synthesis and proliferation of cardiac fibroblasts were also analyzed. Results Immunofluorescence staining and Western blotting showed that the expression levels of Runx3, α-SMA and type I collagen in the fibrosis regions were up-regulated with the extended duration of MI. As compared with the MI 28 d+si-NC group, the MI 28 d+si-Runx3 group had increased left ventricular ejection fraction (LVEF), fractional shortening (FS) and cardiac output (P < 0.05), and decreased left ventricular end-diastolic diameter (LVIDd) and decreased left ventricular (LVIDs) (P < 0.05) in 28 d after MI; the expression levels ...
    Keywords runt related transcription factor 3 ; myocardial fibrosis ; myocardial infarction ; mice ; cardiac fibroblasts ; Medicine (General) ; R5-920
    Subject code 610
    Language Chinese
    Publishing date 2021-12-01T00:00:00Z
    Publisher Editorial Office of Journal of Third Military Medical University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Distribution and Drug Resistance of Pathogenic Bacteria in Diabetic Patients with Double J-Stent Associated Infections.

    Zeng, Ke / Huang, Jun / Li, Xiaobin / Ye, Junbing / Yang, Jian / Zhang, Suchuan

    Infection and drug resistance

    2022  Volume 15, Page(s) 2671–2678

    Abstract: Objective: To analyze the distribution and drug resistance of pathogenic bacteria in diabetic patients with double J-stent associated infections, and to explore the strategies for prevention and treatment of the infections.: Methods: From January ... ...

    Abstract Objective: To analyze the distribution and drug resistance of pathogenic bacteria in diabetic patients with double J-stent associated infections, and to explore the strategies for prevention and treatment of the infections.
    Methods: From January 2019 to December 2021, 266 diabetic patients treated with double J-stent placement in our hospital assessed for eligibility were recruited. Urine and double J-stent samples were collected for pathogenicity assay and screened for biofilm bacteria. Pathogenic bacteria distribution and drug resistance were examined.
    Results: A total of 97 strains (36.5%) of pathogenic bacteria were isolated from urine samples and 129 strains (48.5%) from double J-stent samples (P > 0.05). 3 strains (1.1%) of biofilm bacteria were separated from urine samples and 106 strains (39.8%) from double J-stent samples (P < 0.05). In the double J-stent samples, there were significantly higher ratios of Gram-positive bacteria separated from biofilm bacteria versus the urine-cultured pathogens (44.3%/61.3%, P < 0.05), and higher drug resistance was observed in biofilm bacteria versus urine-cultured pathogens (P < 0.05). Fosfomycin tromethamine showed remarkable susceptibility to both urinary cultured pathogens and double J-stent biofilm bacteria.
    Conclusion: Diabetic patients with double J-stent biofilm-positive bacteria are mainly Gram-positive bacteria, which are prone to biofilm formation and show strong drug resistance.
    Language English
    Publishing date 2022-05-25
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2494856-1
    ISSN 1178-6973
    ISSN 1178-6973
    DOI 10.2147/IDR.S360086
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Impact of job involvement on organizational citizenship behaviors in China

    Zhang, Suchuan

    Journal of business ethics : JOBE Vol. 120, No. 2 , p. 165-174

    2014  Volume 120, Issue 2, Page(s) 165–174

    Author's details Suchuan Zhang
    Keywords Job involvement ; Organizational citizenship behaviors ; Chinese communist party (CCP) ; Party affiliation ; Interaction
    Language English
    Size graph. Darst.
    Publisher Springer
    Publishing place Dordrecht
    Document type Article
    ZDB-ID 868017-6 ; 1478688-6
    ISSN 1573-0697 ; 0167-4544
    ISSN (online) 1573-0697
    ISSN 0167-4544
    Database ECONomics Information System

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  6. Article: Linking protestant work ethic to workplace ostracism

    Zhang, Suchuan / Shi, Qiao

    Journal of investment and management : JIM Vol. 6, No. 2 , p. 66-74

    the mediating effect of subjective well-being

    2017  Volume 6, Issue 2, Page(s) 66–74

    Author's details Suchuan Zhang, Qiao Shi
    Keywords Protestant Work Ethic ; Subjective Well-Being ; Workplace Ostracism
    Language English
    Publisher Science Publishing Group
    Publishing place New York, NY
    Document type Article
    ZDB-ID 2758930-4 ; 2725362-4
    ISSN 2328-7721 ; 2328-7713
    ISSN (online) 2328-7721
    ISSN 2328-7713
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  7. Article: The relationship between subjective well-being and workplace ostracism

    Zhang, Suchuan / Shi, Qiao

    Journal of organizational change management Vol. 30, No. 6 , p. 978-988

    the moderating role of emotional intelligence

    2017  Volume 30, Issue 6, Page(s) 978–988

    Author's details Suchuan Zhang and Qiao Shi
    Keywords Emotional intelligence ; Subjective well-being ; Workplace ostracism ; Organization behaviour
    Language English
    Publisher Emerald Group Publishing Limited
    Publishing place Bingley
    Document type Article
    ZDB-ID 645653-4 ; 2020442-5
    ISSN 0953-4814
    ISSN 0953-4814
    Database ECONomics Information System

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  8. Article: Effect of indwelling time of double J tube on infected ureteral calculi and the distribution of pathogenic characteristics in diabetics.

    Xie, Wenxian / Zhang, Suchuan / Li, Xiaobin / Liu, Yuejiang / Yang, Jian / Liu, Ping / Zeng, Ke

    American journal of translational research

    2021  Volume 13, Issue 5, Page(s) 5685–5690

    Abstract: Objective: To investigate the effect of double J tube indwelling time on infected ureteral calculi (UC) and distribution of pathogenic characteristics in diabetics.: Methods: 132 diabetics with infected UC admitted to our hospital from April 2017 to ... ...

    Abstract Objective: To investigate the effect of double J tube indwelling time on infected ureteral calculi (UC) and distribution of pathogenic characteristics in diabetics.
    Methods: 132 diabetics with infected UC admitted to our hospital from April 2017 to April 2020 were selected. All patients were implanted with a double J tube, followed by percutaneous nephrolithotomy or ureteroscopic holmium laser lithotripsy. According to the indwelling time, they were divided into a research group (≤ 7 d, 60 cases) and a control group (> 7 d, 72 cases). We compared the baseline data, and surgical data of the two groups, and analyzed pathogenic bacteria.
    Results: None of the differences in the operation time, hospital stay, and stone diameter were statistically significant (P > 0.05). Before placement of the double J tube, no striking differences in urinary white blood cells and blood white blood cells were observed between the two groups (P > 0.05). 7 days after the placement of the double J tube, a significant decrease of the urinary white blood cells and blood white blood cells was recorded (P < 0.05), with no significant differences between the two groups (P > 0.05). Before and after placement of double J tube, no striking differences in body temperature > 38.5°C or positive blood culture were observed between the two groups (P > 0.05). Surgical methods, stone removal rate one month after the operation, or incidence of postoperative complications were not significantly different (P > 0.05). 49 pathogenic strains were detected, among which Gram-negative bacteria accounted for 63.27%. The main pathogens were Escherichia coli and Pseudomonas aeruginosa.
    Conclusion: The indwelling time of the double J tube has no significant effect on the effectiveness and safety in diabetic patients with infected UC. It is necessary to reduce the indwelling time and implement targeted stone surgery.
    Language English
    Publishing date 2021-05-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2471058-1
    ISSN 1943-8141
    ISSN 1943-8141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Silenced CXCL12 inhibits ox-LDL-induced foaming and apoptosis in mouse macrophage cell line RAW264.7

    ZHUANG Hong / ZHANG Su-chuan / LIU Hui-di / XU Ya-ning / HUANG Lu / DAI Tian / JIANG Pin

    Jichu yixue yu linchuang, Vol 41, Iss 2, Pp 219-

    2021  Volume 224

    Abstract: Objective To investigate the effect and mechanism of CXC chemokine ligand 12 (CXCL12) on oxidized low density lipoprotein (ox-LDL)-induced foaming and apoptosis of macrophages. Methods RAW264.7 was incubated with 60 mg/L of ox-LDL to induce foaming of ... ...

    Abstract Objective To investigate the effect and mechanism of CXC chemokine ligand 12 (CXCL12) on oxidized low density lipoprotein (ox-LDL)-induced foaming and apoptosis of macrophages. Methods RAW264.7 was incubated with 60 mg/L of ox-LDL to induce foaming of macrophages, which was recorded as ox-LDL group, and to compare with the control without ox-LDL; si-con and si-CXCL12 were transfected to RAW264.7 cells and thenincubated with ox-LDL at 60 mg/L as ox-LDL+si-con group and ox-LDL+si-CXCL12 group, respectively. Oil Red O staining was used to detect cellular foaming; Western blot was used to detect ABCA-1, SRB-1, cleaved caspase-3, cleaved caspase-9, CXCL12, p38 mitogen-activated protein kinase (p38MAPK), and phosphorylated p38MAPK (p-p38MAPK) protein expression; flow cytometry is used to detect apoptosis; CXCL12 mRNA expression was measured by real-time fluorescence quantitative PCR(RT-qPCR). Results The size of ox-LDL-treated macrophages increased, the shape became round, and a large number of red lipid droplets were found in the cytoplasm; the expression of ABCA-1 and SRB-1 and cleaved caspase-3 as well as cleaved caspase-9 were all significantly increased; the apoptosis rate and the expression of CXCL12 and p-p38MAPK were significantly increased(P＜0.05). Silencing CXCL12, no red-stained lipid droplets were found in most cells, only a few cells had partial lipid droplets, the expressions of ABCA-1 and SRB-1 were significantly reduced, and the expression of cleaved caspase-3 and cleaved caspase-9, apoptosis rate and the expression of p-p38MAPK significantly reduced(P＜0.05). Conclusions Silencing CXCL12 inhibits ox-LDL-induced foaming and apoptosis of mouse macrophage cell line RAW264.7, which may be related to the p38MAPK signaling pathway.
    Keywords cxc chemokine ligand 12|oxidized low density lipoprotein|macrophage foaming|apoptosis ; Medicine ; R
    Subject code 630
    Language Chinese
    Publishing date 2021-02-01T00:00:00Z
    Publisher Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Escherichia albertii

    Liu, Qian / Wang, Hong / Zhang, Suchuan / Yan, Guodong / Yang, Xi / Bai, Xiangning / Deng, Jianping / Chen, Xi / Zhang, Ling / Zhang, Jie / Wang, Bin / Zou, Nianli / Xiong, Yanwen / Zhang, Zhengdong

    Heliyon

    2023  Volume 9, Issue 11, Page(s) e22298

    Abstract: A rare case of bacteremia caused ... ...

    Abstract A rare case of bacteremia caused by
    Language English
    Publishing date 2023-11-11
    Publishing country England
    Document type Case Reports
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e22298
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