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  1. Article ; Online: Environmental surveillance reveals co-circulation of distinctive lineages of enteroviruses in southwest China's border cities, 2020-2022.

    Tang, Jingjing / Zhang, Xiaodie / Zhang, Jie / Zhao, Zhixian / Ding, Zhengrong

    Journal of applied microbiology

    2024  Volume 135, Issue 3

    Abstract: Aims: Enteroviruses are significant human pathogens associated with a range of mild to severe diseases. This study aims to understand the diversity and genetic characterization of enteroviruses circulated in southwest China's border cities by using ... ...

    Abstract Aims: Enteroviruses are significant human pathogens associated with a range of mild to severe diseases. This study aims to understand the diversity and genetic characterization of enteroviruses circulated in southwest China's border cities by using environmental surveillance.
    Methods and results: A total of 96 sewage samples were collected in three border cities and a port located in Yunnan Province, China from July 2020 to June 2022. After cell culture and VP1 sequencing, a total of 590 enterovirus isolates were identified, belonging to 21 types. All PV strains were Sabin-like with ≤6 nucleotide mutations in the VP1 coding region. Echovirus 6, echovirus 21 (a rare serotype in previous studies), and coxsackievirus B5 were the predominant serotypes, which accounted for 21.19%, 18.31%, and 13.39% of the total isolates, respectively. The prevalence of the common serotypes varied across different border cities and periods. Phylogenetic analysis revealed the presence of multiple evolutionary lineages for E21, E6, and E30, some of which formed distinct branches.
    Conclusions: High diversity of enteroviruses and distinct lineages of predominant serotypes circulated in southwest China's border cities.
    MeSH term(s) Humans ; Cities ; Phylogeny ; China/epidemiology ; Enterovirus ; Enterovirus Infections/epidemiology ; Enterovirus B, Human/genetics ; Antigens, Viral/genetics ; Environmental Monitoring/methods
    Chemical Substances Antigens, Viral
    Language English
    Publishing date 2024-03-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1358023-1
    ISSN 1365-2672 ; 1364-5072
    ISSN (online) 1365-2672
    ISSN 1364-5072
    DOI 10.1093/jambio/lxae060
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Polyvinyl chloride-based dielectric elastomer with high permittivity and low viscoelasticity for actuation and sensing.

    Huang, Jianjian / Zhang, Xiaodie / Liu, Ruixue / Ding, Yonghui / Guo, Dongjie

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 1483

    Abstract: Dielectric elastomers (DEs) are widely used in soft actuation and sensing. Current DE actuators require high driving electrical fields because of their low permittivity. Most of DE actuators and sensors suffer from high viscoelastic effects, leading to ... ...

    Abstract Dielectric elastomers (DEs) are widely used in soft actuation and sensing. Current DE actuators require high driving electrical fields because of their low permittivity. Most of DE actuators and sensors suffer from high viscoelastic effects, leading to high mechanical loss and large shifts of signals. This study demonstrates a valuable strategy to produce polyvinyl chloride (PVC)-based elastomers with high permittivity and low viscoelasticity. The introduction of cyanoethyl cellulose (CEC) into plasticized PVC gel (PVCg) not only confers a high dielectric permittivity (18.9@1 kHz) but also significantly mitigates their viscoelastic effects with a low mechanical loss (0.04@1 Hz). The CEC/PVCg actuators demonstrate higher actuation performances over the existing DE actuators under low electrical fields and show marginal displacement shifts (7.78%) compared to VHB 4910 (136.09%). The CEC/PVCg sensors display high sensitivity, fast response, and limited signal drifts, enabling their faithful monitoring of multiple human motions.
    Language English
    Publishing date 2023-03-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-37178-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Comparative study of alginate and type I collagen as biomaterials for cartilage stem/progenitor cells to construct tissue-engineered cartilage

    Zhang, Xiaodie / Qi, Lin / Chen, XiaoGang / Lai, Yongxian / Liu, Kai / Xue, Ke

    Frontiers in bioengineering and biotechnology

    2023  Volume 10, Page(s) 1057199

    Abstract: With the help of biomaterials, cartilage stem/progenitor cells (CSPCs) derived from cartilage tissue present a promising choice for cartilage regeneration. In our previous study, we investigated whether CSPCs could be ideal seeding cells for cartilage ... ...

    Abstract With the help of biomaterials, cartilage stem/progenitor cells (CSPCs) derived from cartilage tissue present a promising choice for cartilage regeneration. In our previous study, we investigated whether CSPCs could be ideal seeding cells for cartilage tissue regeneration. Biomaterials are fabricated to accelerate tissue regeneration, providing a suitable environment for cell attachment, proliferation, and differentiation. Among the biomaterials used in cartilage regeneration medicine, alginate and collagen are classified as natural biomaterials and are characterized by high biocompatibility, bioactivity, and non-toxic degradation products. However, it is unclear which material would have a competitive advantage in CSPC-based cartilage regeneration
    Language English
    Publishing date 2023-01-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2022.1057199
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Selective, rapid extraction of uranium from aqueous solution by porous chitosan-phosphorylated chitosan-amidoxime macroporous resin composite and differential charge calculation.

    Zhang, Xiaodie / Zhang, Lieyu / Wang, Qingliang / Xin, Qi / Xiong, Ying / Wang, Hongqiang

    International journal of biological macromolecules

    2023  Volume 253, Issue Pt 1, Page(s) 126661

    Abstract: Herein, a new porous chitosan-phosphorylated chitosan-amidoxime macroporous resin composite (PCAR) was designed and synthesized for the rapid and selective extraction of uranium resources from aqueous solution. This study showed that PCAR exhibited ... ...

    Abstract Herein, a new porous chitosan-phosphorylated chitosan-amidoxime macroporous resin composite (PCAR) was designed and synthesized for the rapid and selective extraction of uranium resources from aqueous solution. This study showed that PCAR exhibited excellent adsorption toward uranium in a pH range of 5-9. The dynamic adsorption process aligned with the quasi-second-order kinetic model and corresponded to the chemical adsorption process. The maximum adsorption capacity was 561.28 mg·g
    MeSH term(s) Uranium/analysis ; Chitosan ; Porosity ; Vanadium ; Hydrogen-Ion Concentration ; Composite Resins ; Adsorption ; Water
    Chemical Substances Uranium (4OC371KSTK) ; Chitosan (9012-76-4) ; amidoxime ; Vanadium (00J9J9XKDE) ; Composite Resins ; Water (059QF0KO0R)
    Language English
    Publishing date 2023-09-03
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2023.126661
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells.

    Xue, Ke / Jiang, Yongkang / Zhang, Xiaodie / Wu, Jun / Qi, Lin / Liu, Kai

    Adipocyte

    2021  Volume 10, Issue 1, Page(s) 322–337

    Abstract: Cartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic ... ...

    Abstract Cartilage tissue engineering is a promising option for repairing cartilage defects, although harvesting a large number of seeding cells remains a major challenge. Cartilage stem/progenitor cells (CSPCs) seem to be a promising cell source. Hypoxic extracellular vesicles (EVs) may play a major role in cell-cell and tissue-tissue communication. In the current study, we aimed to evaluate the effect of hypoxic adipose-derived stem cells (ADSCs)-derived EVs on CSPCs proliferation and differentiation. The characteristics of ADSCs-derived EVs were identified, and proliferation, migration, and cartilage-related gene expression of CSPCs were measured with or without the presence of hypoxic ADSCs-derived EVs. SEM, histological staining, biochemical and biomechanical analysis was performed to evaluate the effect of hypoxic ADSCs-derived EVs on CSPCs in alginate hydrogel culture. The results indicated that the majority of ADSC-derived EVs exhibited a round-shaped or cup-shaped morphology with a diameter of 40-1000 nm and expressed CD9, CD63, and CD81. CSPCs migration and proliferation were enhanced by hypoxic ADSCs-derived EVs, which also increased the expression of cartilage-related genes. The hypoxic ADSCs-derived EVs induce CSPCs to produce significantly more cartilage matrix and proteoglycan. In conclusion, hypoxic ADSCs-derived EVs improved the proliferation and chondrogenic differentiation of CSPCs for cartilage tissue engineering.
    MeSH term(s) Adipose Tissue ; Cartilage ; Cell Differentiation ; Cell Proliferation ; Extracellular Vesicles ; Stem Cells
    Language English
    Publishing date 2021-06-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2681769-X
    ISSN 2162-397X ; 2162-397X
    ISSN (online) 2162-397X
    ISSN 2162-397X
    DOI 10.1080/21623945.2021.1945210
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: RAGE Is a Receptor for SARS-CoV-2 N Protein and Mediates N Protein-induced Acute Lung Injury.

    Xia, Jie / Wang, Jiangmei / Ying, Liyang / Huang, Ruoqiong / Zhang, Kai / Zhang, Ruoyang / Tang, Wenqi / Xu, Qi / Lai, Dengming / Zhang, Yan / Hu, Yaoqin / Zhang, Xiaodie / Zang, Ruoxi / Fan, Jiajie / Shu, Qiang / Xu, Jianguo

    American journal of respiratory cell and molecular biology

    2023  Volume 69, Issue 5, Page(s) 508–520

    Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (N-protein) increases early in body fluids during infection and has recently been identified as a direct inducer for lung injury. However, the signal mechanism of N-protein ...

    Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (N-protein) increases early in body fluids during infection and has recently been identified as a direct inducer for lung injury. However, the signal mechanism of N-protein in the lung inflammatory response remains poorly understood. The goal of this study was to determine whether RAGE (receptor for advanced glycation endproducts) participated in N-protein-induced acute lung injury. The binding between N-protein and RAGE was examined via assays for protein-protein interaction. To determine the signaling mechanism
    MeSH term(s) Animals ; Mice ; Acute Lung Injury/metabolism ; COVID-19 ; NF-kappa B/metabolism ; Receptor for Advanced Glycation End Products/metabolism ; SARS-CoV-2/metabolism
    Chemical Substances NF-kappa B ; Receptor for Advanced Glycation End Products ; Ager protein, mouse
    Language English
    Publishing date 2023-07-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1025960-0
    ISSN 1535-4989 ; 1044-1549
    ISSN (online) 1535-4989
    ISSN 1044-1549
    DOI 10.1165/rcmb.2022-0351OC
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  7. Article: Identification of Hub Genes in the Pathogenesis of Bronchiolitis Obliterans via Bioinformatic Analysis and Experimental Verification.

    Wu, Zhongji / Chen, Xiaowen / Zhang, Kangkang / Liu, Zhenwei / Zhang, Haidi / Zheng, Zhaocong / Zhang, Xiaodie / Chen, Yubiao / Peng, Yinghui / Li, Hui / Huang, Kaiyin / Tang, Sixiang / Zhao, Li / Chen, Dehui

    Journal of inflammation research

    2023  Volume 16, Page(s) 3303–3317

    Abstract: Background: Bronchiolitis obliterans (BO) is a chronic disease that can arise as a complication of severe childhood pneumonia and can also impact the long-term survival of patients after lung transplantation. However, the precise molecular mechanism ... ...

    Abstract Background: Bronchiolitis obliterans (BO) is a chronic disease that can arise as a complication of severe childhood pneumonia and can also impact the long-term survival of patients after lung transplantation. However, the precise molecular mechanism underlying BO remains unclear. We aimed to identify BO-associated hub genes and their molecular mechanisms.
    Methods: BO-associated transcriptome datasets (GSE52761, GSE137169, and GSE94557) were downloaded from the Gene Expression Omnibus (GEO) database to identify differentially expressed genes (DEGs). Additional bioinformatics analyses, such as Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interaction (PPI) analyses, were performed to determine functional roles and DEG-associated regulatory networks. Prediction of hub genes using the 12 algorithms available in the Cytohubba plugin of Cytoscape software was also performed. Verification was performed using the BO mouse model.
    Results: Our results revealed 57 DEGs associated with BO, of which 18 were down-regulated and 39 were up-regulated. The Cytohubba plugin data further narrowed down the 57 DEGs into 9 prominent hub genes (CCR2, CD1D, GM2A, TFEC, MPEG1, CTSS, GPNMB, BIRC2, and CTSZ). Genes such as CCR2, TFEC, MPEG1, CTSS, and CTSZ were dysregulated in 2,3-butanedione-induced BO mice, whereas TFEC, CTSS, and CTSZ were dysregulated in nitric acid-induced BO mouse models.
    Conclusion: Our study identified and validated four novel BO biomarkers, which may allow further investigation into the development of distinct BO diagnostic markers and novel therapeutic avenues.
    Language English
    Publishing date 2023-08-08
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2494878-0
    ISSN 1178-7031
    ISSN 1178-7031
    DOI 10.2147/JIR.S419845
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Transcriptome analysis reveals the impact of NETs activation on airway epithelial cell EMT and inflammation in bronchiolitis obliterans.

    Wu, Zhongji / Chen, Xiaowen / Wu, Shangzhi / Liu, Zhenwei / Li, Hongwei / Mai, Kailin / Peng, Yinghui / Zhang, Haidi / Zhang, Xiaodie / Zheng, Zhaocong / Fu, Zian / Chen, Dehui

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 19226

    Abstract: Bronchiolitis obliterans (BO) is a chronic airway disease that was often indicated by the pathological presentation of narrowed and irreversible airways. However, the molecular mechanisms of BO pathogenesis remain unknown. Although neutrophil ... ...

    Abstract Bronchiolitis obliterans (BO) is a chronic airway disease that was often indicated by the pathological presentation of narrowed and irreversible airways. However, the molecular mechanisms of BO pathogenesis remain unknown. Although neutrophil extracellular traps (NETs) can contribute to inflammatory disorders, their involvement in BO is unclear. This study aims to identify potential signaling pathways in BO by exploring the correlations between NETs and BO. GSE52761 and GSE137169 datasets were downloaded from gene expression omnibus (GEO) database. A series of bioinformatics analyses such as differential expression analysis, gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and gene set enrichment analysis (GSEA) were performed on GSE52761 and GSE137169 datasets to identify BO potential signaling pathways. Two different types of BO mouse models were constructed to verify NETs involvements in BO. Additional experiments and bioinformatics analysis using human small airway epithelial cells (SAECs) were also performed to further elucidate differential genes enrichment with their respective signaling pathways in BO. Our study identified 115 differentially expressed genes (DEGs) that were found up-regulated in BO. Pathway enrichment analysis revealed that these genes were primarily involved in inflammatory signaling processes. Besides, we found that neutrophil extracellular traps (NETs) were formed and activated during BO. Our western blot analysis on lung tissue from BO mice further confirmed NETs activation in BO, where neutrophil elastase (NE) and myeloperoxidase (MPO) expression were found significantly elevated. Transcriptomic and bioinformatics analysis of NETs treated-SAECs also revealed that NETs-DEGs were primarily associated through inflammatory and epithelial-to-mesenchymal transition (EMT) -related pathways. Our study provides novel clues towards the understanding of BO pathogenesis, in which NETs contribute to BO pathogenesis through the activation of inflammatory and EMT associated pathways. The completion of our study will provide the basis for potential novel therapeutic targets in BO treatment.
    MeSH term(s) Humans ; Mice ; Animals ; Extracellular Traps/metabolism ; Gene Expression Profiling ; Transcriptome ; Bronchiolitis Obliterans/metabolism ; Inflammation ; Epithelial Cells/metabolism ; Computational Biology
    Language English
    Publishing date 2023-11-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-45617-y
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  9. Article ; Online: Implementation of a motivational programme based on existence- relatedness-growth theory in nursing undergraduate interns: A quasi-experimental study.

    Yang, Shuai / Zhang, Xiao-Die / Guo, Wei / Ho, Ka Yan / Lam, Katherine Ka Wai / Dong, Yan-Yan / Pan, Jing-Da / Liu, Qi / Xu, Ri-Di / Ling, Dong-Lan / Yu, Hong-Jing

    Nurse education today

    2023  Volume 129, Page(s) 105894

    Abstract: Background: Currently, a paramount issue in nursing education is to motivate nursing undergraduate interns to develop self-directed learning skills and improve their practice satisfaction and professional identity, so as to meet the growing demands in ... ...

    Abstract Background: Currently, a paramount issue in nursing education is to motivate nursing undergraduate interns to develop self-directed learning skills and improve their practice satisfaction and professional identity, so as to meet the growing demands in healthcare.
    Objectives: This study aimed to examine the effectiveness of a motivational programme based on the Existence-Relatedness-Growth (ERG) theory in developing self-directed learning skills, improving practice satisfaction and promoting the professional identity of nursing undergraduate interns in China.
    Design: A quasi-experimental study design.
    Setting: A government-funded tertiary teaching hospital in Guangzhou, Guangdong province, China.
    Methods: This study was conducted with 99 nursing undergraduate interns in a hospital between June 2020 and April 2022. The interns in the experimental group (n = 50) participated in the motivational programme based on ERG theory, while those in the control group (n = 49) underwent a traditional training programme. The interns in the two groups were compared in terms of their degree of self-directed learning, practice satisfaction and professional identity after the training, using independent samples t-test.
    Results: After the internship, interns in the experimental group showed a statistically significantly higher level of self-directed learning and practice satisfaction than those in the control group (p < 0.05). However, no significant difference was observed in professional identity between the two groups after the internship.
    Conclusions: The motivational programme based on ERG theory was shown to be effective in improving self-directed learning and practice satisfaction in nursing undergraduate interns. A large-scale randomized controlled trial is warranted to confirm the results.
    MeSH term(s) Humans ; Education, Nursing ; Internship and Residency ; Learning ; Delivery of Health Care ; Students, Nursing
    Language English
    Publishing date 2023-07-05
    Publishing country Scotland
    Document type Journal Article
    ZDB-ID 1062570-7
    ISSN 1532-2793 ; 0260-6917
    ISSN (online) 1532-2793
    ISSN 0260-6917
    DOI 10.1016/j.nedt.2023.105894
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  10. Article ; Online: Exosomes derived from mature chondrocytes facilitate subcutaneous stable ectopic chondrogenesis of cartilage progenitor cells.

    Chen, Yahong / Xue, Ke / Zhang, Xiaodie / Zheng, Zhiwei / Liu, Kai

    Stem cell research & therapy

    2018  Volume 9, Issue 1, Page(s) 318

    Abstract: Background: Developing cartilage constructed with the appropriate matrix composition and persistent chondrogenesis remains an enduring challenge in cartilage defects. Cartilage progenitor cell (CPC)-based tissue engineering has attracted recent ... ...

    Abstract Background: Developing cartilage constructed with the appropriate matrix composition and persistent chondrogenesis remains an enduring challenge in cartilage defects. Cartilage progenitor cell (CPC)-based tissue engineering has attracted recent attention because of its strong chondrogenic differentiation capacity. However, due to the lack of a suitable chondrogenic niche, the clinical application of CPC-regenerated cartilage in the subcutaneous environment remains a challenge. In this study, exosomes derived from chondrocytes (CC-Exos) were used to provide the CPC constructs with a cartilage signal in subcutaneous environments for efficient ectopic cartilage regeneration.
    Methods: Rabbit CPC-alginate constructs were prepared and implanted subcutaneously in nude mice. CC-Exos were injected into the constructs at the same dose (30 μg exosomes per 100 μL injection) after surgery and thereafter weekly for a period of 12 weeks. Exosomes derived from bone mesenchymal stem cells (BMSC-Exos) were used as the positive control. The mice in the negative control were administered with the same volume of PBS. At 4 and 12 weeks after implantation, the potential of CC-Exos and BMSC-Exos to promote chondrogenesis and stability of cartilage tissue in a subcutaneous environment were analyzed by histology, immunostaining, and protein analysis. The influences of BMSC-Exos and CC-Exos on chondrogenesis and angiogenic characteristics in vitro were assessed via coculturing with CPCs and human umbilical vein endothelial cells.
    Results: The CC-Exos injection increased collagen deposition and minimized vascular ingrowth in engineered constructs, which efficiently and reproducibly developed into cartilage. The generated cartilage was phenotypically stable with minimal hypertrophy and vessel ingrowth up to 12 weeks, while the cartilage formed with BMSC-Exos was characterized by hypertrophic differentiation accompanied by vascular ingrowth. In vitro experiments indicated that CC-Exos stimulated CPCs proliferation and increased expression of chondrogenesis markers while inhibiting angiogenesis.
    Conclusions: These findings suggest that the novel CC-Exos provides the preferable niche in directing stable ectopic chondrogenesis of CPCs. The use of CC-Exos may represent an off-the-shelf and cell-free therapeutic approach for promoting cartilage regeneration in the subcutaneous environment.
    MeSH term(s) Animals ; Cartilage/cytology ; Cartilage/physiology ; Chondrocytes/chemistry ; Chondrocytes/metabolism ; Chondrogenesis/physiology ; Exosomes/chemistry ; Exosomes/metabolism ; Female ; Heterografts ; Humans ; Injections, Subcutaneous ; Mesenchymal Stem Cells/chemistry ; Mesenchymal Stem Cells/metabolism ; Mice ; Mice, Nude ; Rabbits ; Regeneration/physiology ; Stem Cells/cytology
    Language English
    Publishing date 2018-11-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2548671-8
    ISSN 1757-6512 ; 1757-6512
    ISSN (online) 1757-6512
    ISSN 1757-6512
    DOI 10.1186/s13287-018-1047-2
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