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Article ; Online: Current status of common cold-related knowledge, attitude, and practices among nursing students as well as relevant factors in China.

Shi, Hui-Min / Si, Lian-Jing / Zhang, Yu-Wen

2024 Volume 103, Issue 5, Page(s) e37129

Abstract: To understand the current status of cold-related knowledge, attitude, and practices (KAP) among nursing students as well as relevant factors, and provide a scientific basis for offering effective health education and promoting health care levels among ... ...

Abstract	To understand the current status of cold-related knowledge, attitude, and practices (KAP) among nursing students as well as relevant factors, and provide a scientific basis for offering effective health education and promoting health care levels among nursing students. We conducted a survey of 668 nursing students using a self-designed "General Condition Questionnaire" and "Survey of common cold-related knowledge, attitude, and practices among students majoring in nursing." We used SPSS22.0 for data sorting and analysis; mean ± standard deviation was used for statistical description of the questionnaire scores, and t-test and ANOVA (analysis of variance) were used for differences comparison between binary variables and polytomous variables of the relevant factors. The test level α was 0.05, and the difference was considered statistically significant when P < .05. The total KAP score of the nursing students was 128.47 ± 13.91 points, which is a good level. There were significant differences in the KAP scores based on gender, educational background, grade, whether relevant knowledge had been acquired, extracurricular activities, weekly exercise frequency, exercise time per session, coping method after catching a cold, and medicine purchase methods (P < .05). The KAP level for cold among nursing students is at a good level. It is recommended that the relevant departments of nursing schools strengthen the education of nursing students on the knowledge about cold and physical exercise.
MeSH term(s)	Humans ; Students, Nursing ; Health Knowledge, Attitudes, Practice ; Common Cold ; Surveys and Questionnaires ; China
Language	English
Publishing date	2024-02-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000037129
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Article ; Online: Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid

Zhang, Yu-Wen / Shi, Yu-Cen / Zhang, Shi-Bao

Plant diversity

2022 Volume 45, Issue 3, Page(s) 326–336

Abstract: Dendrobium ... ...

Abstract	Dendrobium nobile
Language	English
Publishing date	2022-10-27
Publishing country	China
Document type	Journal Article
ZDB-ID	2873411-7
ISSN	2468-2659 ; 2095-0845
ISSN (online)	2468-2659
ISSN	2095-0845
DOI	10.1016/j.pld.2022.10.004
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Article ; Online: Quantitation of RhoA activation: differential binding to downstream effectors.

Zhang, Yu-Wen / Torsilieri, Holly M / Casanova, James E

Small GTPases

2022 Volume 13, Issue 1, Page(s) 296–306

Abstract: The small GTPase RhoA controls many important cellular processes through its ability to activate multiple downstream effector pathways. Most RhoA effectors contain a Rho-binding domain (RBD), and interaction between active RhoA and the RBD typically ... ...

Abstract	The small GTPase RhoA controls many important cellular processes through its ability to activate multiple downstream effector pathways. Most RhoA effectors contain a Rho-binding domain (RBD), and interaction between active RhoA and the RBD typically induces a conformational change in effectors that stimulates their recruitment or activity. Isolated GTPase binding domains fused to GST have been widely used in so-called pulldown assays to measure the activation state of other GTPases in cell lysates. Similarly, GST fusions containing the RBD of the RhoA effector Rhotekin have been widely adopted as a standardized tool for the measurement of RhoA activation. RBDs have also been used to generate fluorescent reporter constructs to localize sites of GTPase activation in intact cells. In this report, we demonstrate that not all forms of active RhoA are capable of interacting with the Rhotekin RBD. A constitutively active RhoA-G14V mutant, which interacted with the RBDs of ROCK2 and mDIA1, was unable to bind the Rhotekin RBD as evidenced by both conventional GST pulldown assay and our newly established BRET assay. Furthermore, active RhoA induced by different stimuli in cells also displayed binding preference for its diverse effectors. Our data demonstrate that RhoA may undergo effector-specific activation for differential regulation of its downstream pathways, and that RhoA activation should not be defined solely by its interaction with Rhotekin.
MeSH term(s)	Protein Binding ; rhoA GTP-Binding Protein/metabolism
Chemical Substances	rhoA GTP-Binding Protein (EC 3.6.5.2)
Language	English
Publishing date	2022-11-05
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2682247-7
ISSN	2154-1256 ; 2154-1248
ISSN (online)	2154-1256
ISSN	2154-1248
DOI	10.1080/21541248.2022.2111945
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Article: Promise and challenges on the horizon of MET-targeted cancer therapeutics.

Zhang, Yu-Wen

World journal of biological chemistry

2015 Volume 6, Issue 2, Page(s) 16–27

Abstract: MET (MNNG HOS transforming gene) is one of the receptor tyrosine kinases whose activities are frequently altered in human cancers, and it is a promising therapeutic target. MET is normally activated by its lone ligand, hepatocyte growth factor (HGF), ... ...

Abstract	MET (MNNG HOS transforming gene) is one of the receptor tyrosine kinases whose activities are frequently altered in human cancers, and it is a promising therapeutic target. MET is normally activated by its lone ligand, hepatocyte growth factor (HGF), eliciting its diverse biological activities that are crucial for development and physiology. Alteration of the HGF-MET axis results in inappropriate activation of a cascade of intracellular signaling pathways that contributes to hallmark cancer events including deregulated cell proliferation and survival, angiogenesis, invasion, and metastasis. Aberrant MET activation results from autocrine or paracrine mechanisms due to overexpression of HGF and/or MET or from a ligand-independent mechanism caused by activating mutations or amplification of MET. The literature provides compelling evidence for the role of MET signaling in cancer development and progression. The finding that cancer cells often use MET activation to escape therapies targeting other pathways strengthens the argument for MET-targeted therapeutics. Diverse strategies have been explored to deactivate MET signaling, and compounds and biologics targeting the MET pathway are in clinical development. Despite promising results from various clinical trials, we are still waiting for true MET-targeted therapeutics in the clinic. This review will explore recent progress and hurdles in the pursuit of MET-targeted cancer drugs and discuss the challenges in such development.
Language	English
Publishing date	2015-05-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2564793-3
ISSN	1949-8454
ISSN	1949-8454
DOI	10.4331/wjbc.v6.i2.16
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Article: Intracellular activated logic nanomachines based on framework nucleic acids for low background detection of microRNAs in living cells.

Li, Xiao-Qiong / Jia, Yi-Lei / Zhang, Yu-Wen / Chen, Hong-Yuan / Xu, Jing-Juan

Chemical science

2023 Volume 14, Issue 28, Page(s) 7699–7708

Abstract: DNA molecular machines based on DNA logic circuits show unparalleled potential in precision medicine. However, delivering DNA nanomachines into real biological systems and ensuring that they perform functions specifically, quickly and logically remain a ... ...

Abstract	DNA molecular machines based on DNA logic circuits show unparalleled potential in precision medicine. However, delivering DNA nanomachines into real biological systems and ensuring that they perform functions specifically, quickly and logically remain a challenge. Here, we developed an efficient DNA molecular machine integrating transfer-sensor-computation-output functions to achieve high fidelity detection of intracellular biomolecules. The introduction of pH nanoswitches enabled the nanomachines to be activated after entering the cell, and the spatial-confinement effect of the DNA triangular prism (TP) enables the molecular machine to process complex information at the nanoscale, with higher sensitivity and shorter response time than diffuse-dominated logic circuits. Such cascaded activation molecular machines follow the logic of AND to achieve specific capture and detection of biomolecules in living cells through a multi-hierarchical response, providing a new insight into the construction of efficient DNA molecular machines.
Language	English
Publishing date	2023-06-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2559110-1
ISSN	2041-6539 ; 2041-6520
ISSN (online)	2041-6539
ISSN	2041-6520
DOI	10.1039/d3sc01162c
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Article ; Online: Age-related differences in physiological and metabolic responses of Pleione aurita (Orchidaceae) pseudobulbs to drought stress and recovery.

Zhang, Wei / Dong, Xiu-Mei / Zhang, Yu-Wen / Fan, Ze-Xin / Zhang, Shi-Bao

Plant physiology and biochemistry : PPB

2023 Volume 197, Page(s) 107655

Abstract: The pseudobulb is a storage organ for water and nutrients that plays a crucial role in the growth and survival of epiphytic orchids. However, the role of water and metabolites in pseudobulb during adaptation to environmental stress are rarely detected ... ...

Abstract	The pseudobulb is a storage organ for water and nutrients that plays a crucial role in the growth and survival of epiphytic orchids. However, the role of water and metabolites in pseudobulb during adaptation to environmental stress are rarely detected through control experiments. In the present study, water-related physiological traits and metabolite changes in the pseudobulbs at the flowering stage and full leaf expansion stage for Pleione aurita were investigated after drought stress and recovery treatments. We found that the composition of non-structural carbohydrates (starch vs. soluble sugar) varied over the lifetime of pseudobulbs, and older pseudobulbs stored more water, whereas younger pseudobulbs stored more dry matter. When plants were subjected to drought stress and subsequent recovery, multiple metabolites in the pseudobulbs including non-structural carbohydrates, flavonoids, phenolic acids, as well as amino acids and their derivatives responded positively to these water level fluctuations. For those metabolites that differently accumulated in both stress and recovery processes, old pseudobulbs contained a higher number of these key metabolites than did the connected younger pseudobulbs. In addition, young and old pseudobulbs use different metabolic pathways to both respond and recover to drought. These results indicate that orchid pseudobulbs cope with water level fluctuations by mobilizing metabolite reserves and that pseudobulbs of different ages exhibit different physiological and metabolic responses to drought stress. These findings broadens our understanding of the role pseudobulbs play in the survival of orchids growing in epiphytic habitats.
MeSH term(s)	Orchidaceae/metabolism ; Droughts ; Plant Leaves/metabolism ; Carbohydrates ; Water/metabolism ; Stress, Physiological
Chemical Substances	Carbohydrates ; Water (059QF0KO0R)
Language	English
Publishing date	2023-03-23
Publishing country	France
Document type	Journal Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2023.107655
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Article ; Online: Endogenous AND Logic DNA Nanomachine for Highly Specific Cancer Cell Imaging.

Zhang, Yu-Wen / Wang, Shu-Min / Li, Xiao-Qiong / Kang, Bin / Chen, Hong-Yuan / Xu, Jing-Juan

Analytical chemistry

2024 Volume 96, Issue 18, Page(s) 7030–7037

Abstract: Intracellular cancer-related biomarker imaging strategy has been used for specific identification of cancer cells, which was of great importance to accurate cancer clinical diagnosis and prognosis studies. Localized DNA circuits with improved sensitivity ...

Abstract	Intracellular cancer-related biomarker imaging strategy has been used for specific identification of cancer cells, which was of great importance to accurate cancer clinical diagnosis and prognosis studies. Localized DNA circuits with improved sensitivity showed great potential for intracellular biomarkers imaging. However, the ability of localized DNA circuits to specifically image cancer cells is limited by off-site signal leakage associated with a single-biomarker sensing strategy. Herein, we integrated the endogenous enzyme-powered strategy with logic-responsive and localized signal amplifying capability to construct a self-assembled endogenously AND logic DNA nanomachine (EDN) for highly specific cancer cell imaging. When the EDN encountered a cancer cell, the overexpressed DNA repairing enzyme apurinic/apyrimidinic endonuclease 1 (APE1) and miR-21 could synergistically activate a DNA circuit via cascaded localized toehold-mediated strand displacement (TMSD) reactions, resulting in amplified fluorescence resonance energy transfer (FRET) signal. In this strategy, both endogenous APE1 and miR-21, served as two "keys" to activate the AND logic operation in cancer cells to reduce off-tumor signal leakage. Such a multiplied molecular recognition/activation nanomachine as a powerful toolbox realized specific capture and reliable imaging of biomolecules in living cancer cells.
MeSH term(s)	Humans ; MicroRNAs/analysis ; MicroRNAs/metabolism ; DNA/chemistry ; DNA-(Apurinic or Apyrimidinic Site) Lyase/metabolism ; Fluorescence Resonance Energy Transfer ; Neoplasms/diagnostic imaging ; Optical Imaging
Chemical Substances	MicroRNAs ; MIRN21 microRNA, human ; DNA (9007-49-2) ; DNA-(Apurinic or Apyrimidinic Site) Lyase (EC 4.2.99.18) ; APEX1 protein, human (EC 4.2.99.18)
Language	English
Publishing date	2024-04-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.4c00211
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Article: S-propargyl-cysteine delays the progression of atherosclerosis and increases eNOS phosphorylation in endothelial cells.

Li, Zhi-Ming / Li, Ping / Zhu, Lei / Zhang, Yu-Wen / Zhu, Yi-Chun / Wang, He / Yu, Bo / Wang, Ming-Jie

Sheng li xue bao : [Acta physiologica Sinica

2023 Volume 75, Issue 3, Page(s) 317–327

Abstract: The present study aimed to investigate the protective effect of S-propargyl-cysteine (SPRC) on atherosclerosis progression in mice. A mouse model of vulnerable atherosclerotic plaque was created in ... ...

Abstract	The present study aimed to investigate the protective effect of S-propargyl-cysteine (SPRC) on atherosclerosis progression in mice. A mouse model of vulnerable atherosclerotic plaque was created in ApoE
MeSH term(s)	Animals ; Humans ; Mice ; Atherosclerosis ; Cholesterol/metabolism ; Cysteine/pharmacology ; Human Umbilical Vein Endothelial Cells/metabolism ; Lipoproteins, LDL/pharmacology ; Nitric Oxide Synthase Type III/genetics ; Nitric Oxide Synthase Type III/metabolism ; Phosphorylation ; Plaque, Atherosclerotic/metabolism ; Plaque, Atherosclerotic/pathology
Chemical Substances	Cholesterol (97C5T2UQ7J) ; Cysteine (K848JZ4886) ; Lipoproteins, LDL ; Nitric Oxide Synthase Type III (EC 1.14.13.39)
Language	English
Publishing date	2023-06-20
Publishing country	China
Document type	Journal Article
ZDB-ID	604308-2
ISSN	0371-0874
ISSN	0371-0874
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Article ; Online: Long-term grazing improved soil chemical properties and benefited community traits under climatic influence in an alpine typical steppe.

Zhang, Yu-Wen / Peng, Ze-Chen / Chang, Sheng-Hua / Wang, Zhao-Feng / Li, Duo-Cai / An, Yu-Feng / Hou, Fu-Jiang / Ren, Ji-Zhou

Journal of environmental management

2023 Volume 348, Page(s) 119184

Abstract: Grazing and climate change both contribute to diversity loss and productivity fluctuations. Sensitive climate conditions and long-term grazing activities have a profound influence on community change, particularly in high-altitude mountain grassland ... ...

Abstract	Grazing and climate change both contribute to diversity loss and productivity fluctuations. Sensitive climate conditions and long-term grazing activities have a profound influence on community change, particularly in high-altitude mountain grassland ecosystems. However, knowledge about the role of long-term continuous grazing management on diversity, productivity and the regulation mechanisms in fragile grassland ecosystems is still rudimentary. We conducted a long-term grazing experiment on an alpine typical steppe in the Qilian Mountains to assess effects of grazing intensity on soil, diversity, productivity and the regulation mechanisms. Plants and soil were sampled along grazing gradients at different distances from the pasture entrance (0, 0.3, 0.6, 0.9, 1.2 and 1.5 km) under the non-growing (WP) and the growing season grazing pasture (SAP). The results revealed that community diversity and biomass did not change significantly on a time scale, while the concentration of soil organic carbon and total phosphorus increased significantly. Heavy grazing (0-0.3 km) decreased community diversity and biomass. Grazing increased soil chemical properties in heavy grazed areas of WP, while the opposite was recorded in SAP. Soil chemical properties explained the largest variances in community diversity and community biomass. The prediction model indicates that grazing in WP mainly affects community diversity through soil chemical properties, and promotes a positive correlation between community diversity and community biomass; in SAP, the direct effect of grazing gradients on community diversity and biomass is the main pathway, but not eliminating the single positive relationship between diversity and biomass, which means that diversity can still be used as a potential resource to promote productivity improvement. Therefore, we should focus on the regulation of soil chemical properties in WP, such as the health and quality of soil, strengthening its ability to store water, sequester carbon and increase nutrients; focus on the management of livestock in SAP, including providing fertilizer and sowing to increase diversity and production in heavily grazed regions and reducing grazing pressure through regional rotational grazing. Ultimately, we call for strengthening the stability and sustainability of ecosystems through targeted and active human intervention in ecologically sensitive areas to cope with future grazing pressures and climate disturbances.
MeSH term(s)	Humans ; Ecosystem ; Grassland ; Soil/chemistry ; Carbon ; Biomass
Chemical Substances	Soil ; Carbon (7440-44-0)
Language	English
Publishing date	2023-10-11
Publishing country	England
Document type	Journal Article
ZDB-ID	184882-3
ISSN	1095-8630 ; 0301-4797
ISSN (online)	1095-8630
ISSN	0301-4797
DOI	10.1016/j.jenvman.2023.119184
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Article ; Online: An intelligent DNA nanomachine for amplified MicroRNA imaging and MicroRNA-Guided efficient gene silencing.

Zhang, Yu-Wen / Li, Shan / Wang, Shu-Min / Li, Xiao-Qiong / Cui, Mei-Rong / Kang, Bin / Chen, Hong-Yuan / Xu, Jing-Juan

Talanta

2023 Volume 265, Page(s) 124820

Abstract: The DNA nanomachines as excellent synthetic biological tools have been widely used for the sensitive detection of intracellular microRNA (miRNA) and DNAzyme-involved gene silencing. However, intelligent DNA nanomachines which have the ability to sense ... ...

Abstract	The DNA nanomachines as excellent synthetic biological tools have been widely used for the sensitive detection of intracellular microRNA (miRNA) and DNAzyme-involved gene silencing. However, intelligent DNA nanomachines which have the ability to sense intracellular specific biomolecules and respond to external information in complex environments still remain challenging. Herein, we develop a miRNA-responsive DNAzyme cascaded catalytic (MDCC) nanomachine to perform multilayer cascade reactions, enabling the amplified intracellular miRNA imaging and miRNA-guided efficient gene silencing. The intelligent MDCC nanomachine is designed based on multiple DNAzyme subunit-encoded catalyzed hairpin assembly (CHA) reactants sustained by the pH-responsive Zeolitic imidazolate framework-8 (ZIF-8) nanoparticles. After cellular uptake, the MDCC nanomachine degrades in acidic endosome and releases three hairpin DNA reactants and Zn
MeSH term(s)	MicroRNAs/genetics ; DNA, Catalytic/metabolism ; DNA ; Catalysis ; RNA, Messenger ; Biosensing Techniques/methods
Chemical Substances	MicroRNAs ; DNA, Catalytic ; DNA (9007-49-2) ; RNA, Messenger
Language	English
Publishing date	2023-06-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2023.124820
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