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Article ; Online: Iontronic microscopy of a tungsten microelectrode: "seeing" ionic currents under an optical microscope.

Zhang, Zhu / Faez, Sanli

Faraday discussions

2023 Volume 246, Page(s) 426–440

Abstract: Optical methods for monitoring electrochemical reactions at an interface are advantageous because of their table-top setup and ease of integration into reactors. Here we apply EDL-modulation microscopy to one of the main components of amperometric ... ...

Abstract	Optical methods for monitoring electrochemical reactions at an interface are advantageous because of their table-top setup and ease of integration into reactors. Here we apply EDL-modulation microscopy to one of the main components of amperometric measurement devices: a microelectrode. We present experimental measurements of the EDL-modulation contrast from the tip of a tungsten microelectrode at various electrochemical potentials inside a ferrocene-dimethanol Fe(MeOH)
Language	English
Publishing date	2023-10-12
Publishing country	England
Document type	Journal Article
ISSN	1364-5498
ISSN (online)	1364-5498
DOI	10.1039/d3fd00040k
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Comparative hemolymph proteomic analyses of the freezing and resistance-freezing Ostrinia furnacalis (Guenée).

Zhang, Zhu-Ting / Wang, Huan / Dong, Hui / Cong, Bin

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2580

Abstract: The Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae), is one of the most harmful pests of maize in Asia. It poses a significant threat to maize production, causing economic losses due to its strong ecological adaptation. In this ... ...

Abstract	The Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae), is one of the most harmful pests of maize in Asia. It poses a significant threat to maize production, causing economic losses due to its strong ecological adaptation. In this study, we compared and analyzed the hemolymph proteome between freezing and resistance-freezing O. furnacalis strains using two-dimensional gel electrophoresis to gain insights into the mechanisms of cold resistance. The results revealed that 300-400 hemolymph protein spots were common, with 24 spots showing differences between the two strains. Spectrometry analysis revealed 21 protein spots, including 17 upregulated spots and 4 downregulated ones. The expression of upregulation/downregulation proteins plays a crucial role in the metabolism, energy supply, and defense reaction of insects. Proteomics research not only provides a method for investigating protein expression patterns but also identifies numerous attractive candidates for further exploration.
MeSH term(s)	Animals ; Larva/physiology ; Hemolymph ; Freezing ; Proteomics ; Moths/physiology ; Zea mays
Language	English
Publishing date	2024-01-31
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52792-z
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Article ; Online: Parameter adaptive sliding mode trajectory tracking strategy with initial value identification for the swing in a hydraulic construction robot.

Hou, Jing-Wei / Ni, Tao / Zhang, Zhu-Xin

Scientific reports

2023 Volume 13, Issue 1, Page(s) 5855

Abstract: A novel trajectory tracking strategy is developed for a double actuated swing in a hydraulic construction robot. Specifically, a nonlinear hydraulic dynamics model of a double actuated swing is established, and a parameter adaptive sliding mode control ... ...

Abstract	A novel trajectory tracking strategy is developed for a double actuated swing in a hydraulic construction robot. Specifically, a nonlinear hydraulic dynamics model of a double actuated swing is established, and a parameter adaptive sliding mode control strategy is designed to enhance the trajectory tracking performance. When an object is grabbed and unloaded, the moment of inertia of a swing considerably changes, and the performance of the estimation algorithm is generally inadequate. Thus, it is necessary to establish an algorithm to identify the initial value of the moment of inertia of the object. To this end, this paper proposes a novel initial value identification algorithm based on a two-DOF robot gravity force identification method combined with stereo vision information. The performance of the identification algorithm is enhanced. Simulations and experiments are performed to verify the effect of the novel control scheme.
Language	English
Publishing date	2023-04-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-30952-x
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Article ; Online: Dark-field light scattering microscope with focus stabilization.

Peters, Anna / Zhang, Zhu / Faez, Sanli

HardwareX

2023 Volume 14, Page(s) e00424

Abstract: We present detailed design and operation instructions for a single-objective inverted microscope. Our design is suitable for two dark-field modes of operation: 1- total internal reflection scattering, and 2- cross-polarization backscattering. The user ... ...

Abstract	We present detailed design and operation instructions for a single-objective inverted microscope. Our design is suitable for two dark-field modes of operation: 1- total internal reflection scattering, and 2- cross-polarization backscattering. The user can switch between the two modes by exchanging one mode-steering element, which is also adapted to the Thorlabs cage system. To establish a stable background speckle for differential microscopy the imaging plane is stabilized with active feedback. We validate the stabilization efficacy by performing long-term scattering measurement on single nanoparticles. This setup can be extended for simultaneous scattering, fluorescence, and confocal imaging modes.
Language	English
Publishing date	2023-05-12
Publishing country	England
Document type	Journal Article
ISSN	2468-0672
ISSN (online)	2468-0672
DOI	10.1016/j.ohx.2023.e00424
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Article: Effect of Microstructure on High-Speed Tensile Mechanical Properties of Ti-1300 Alloy.

Zhang, Zhu-Ye / Liu, Dong-Rong / Pu, Zhen-Peng

Materials (Basel, Switzerland)

2023 Volume 16, Issue 13

Abstract: It is usually required that Ti-1300 alloys be able to withstand a greater load under special conditions, such as the controllable collision of a space shuttle and rapid collision of an automobile. Because of a good combination of strength and toughness, ... ...

Abstract	It is usually required that Ti-1300 alloys be able to withstand a greater load under special conditions, such as the controllable collision of a space shuttle and rapid collision of an automobile. Because of a good combination of strength and toughness, Ti-1300 alloys are widely applied in the aerospace industry. However, during the service process, the alloy components inevitably bear extreme loads. This paper uses high-speed tensile technology to systematically study the effects of different strain rates on the deformation of the microstructure and deformation mechanism of Ti-1300 alloys and to clarify a relation between the microstructure and mechanical properties. The results show that no phase transformation occurs during the high-speed tensile process at strain rates of 200 s
Language	English
Publishing date	2023-06-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma16134725
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Article ; Online: XAV939 Improves the Prognosis of Myocardial Infarction by Blocking the Wnt/β-Catenin Signalling Pathway.

Zhang, Zhu / Qi, Jiancheng / Fan, Xiucai / Pan, Min

Applied biochemistry and biotechnology

2023 Volume 196, Issue 2, Page(s) 605–615

Abstract: Myocardial infarction (MI) is closely related to the Wnt signalling pathway, but the role of XAV939 (a Wnt/β-catenin signalling pathway blocker) in MI has not been elucidated. The purpose of this study was to explore the role of XAV939 in mouse hearts ... ...

Abstract	Myocardial infarction (MI) is closely related to the Wnt signalling pathway, but the role of XAV939 (a Wnt/β-catenin signalling pathway blocker) in MI has not been elucidated. The purpose of this study was to explore the role of XAV939 in mouse hearts and to provide a new and feasible treatment for improving the prognosis of MI. C57BL/6 (male, 8 weeks old, 20-25 g) mice were selected for our study. The MI model was made by ligating the left anterior descending coronary artery. On day 28 after the operation, cardiac function was examined by echocardiography. Infarct size, fibrosis, and angiogenesis were individually measured by TTC assays, Masson's trichrome staining, and CD31 analysis, respectively. Apoptosis was examined by TdT-mediated dUTP nick-end labelling (TUNEL) staining. The expression of Wnt, β-catenin, caspase 3, Bax, and Bcl-2 was determined by western blotting. XAV939 successfully blocked Wnt/β-catenin signalling pathway activation in cardiomyocytes after MI by promoting the degradation of β-catenin. XAV939 suppressed fibrosis and apoptosis, promoted angiogenesis, reduced myocardial infarct size and improved cardiac function after MI. XAV939 can reduce myocardial infarct size and improve cardiac function by blocking the Wnt/β-catenin signalling pathway, which may provide a new strategy for improving the prognosis of MI.
MeSH term(s)	Male ; Mice ; Animals ; Myocardium/metabolism ; beta Catenin/metabolism ; Mice, Inbred C57BL ; Myocardial Infarction/drug therapy ; Wnt Signaling Pathway ; Prognosis ; Fibrosis ; Apoptosis ; Disease Models, Animal ; Heterocyclic Compounds, 3-Ring
Chemical Substances	XAV939 ; beta Catenin ; Heterocyclic Compounds, 3-Ring
Language	English
Publishing date	2023-05-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	392344-7
ISSN	1559-0291 ; 0273-2289
ISSN (online)	1559-0291
ISSN	0273-2289
DOI	10.1007/s12010-023-04485-y
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Article: HCP5 Promotes Proliferation and Contributes to Cisplatin Resistance in Gastric Cancer Through miR-519d/HMGA1 Axis.

Zhang, Zhu / Wang, Huahong

Cancer management and research

2021 Volume 13, Page(s) 787–794

Abstract: Introduction: The long-non-coding RNA HCP5 (HLA complex P5) has been extensively linked to the ability of cancer cells to resist chemotherapeutic interventions. Here, we investigated the role of HCP5 in gastric cancer (GC) which to-date has been poorly ... ...

Abstract	Introduction: The long-non-coding RNA HCP5 (HLA complex P5) has been extensively linked to the ability of cancer cells to resist chemotherapeutic interventions. Here, we investigated the role of HCP5 in gastric cancer (GC) which to-date has been poorly characterized. Our results indicated that HCP5 expression was up-regulated in GC cells. Methods: HCP5, miR-519d, and high mobility group A1 (HMGA1) expression levels in GC cells were measured using quantitative real-time PCR (qRT-PCR) and Western blot analysis. Drug sensitivity and apoptosis of tumor cells were assessed using cell counting kit-8, flow cytometry, and caspase activity assay. Bioinformatics and luciferase reporter assays were employed for analyzing the interactions between HCP5, miR-519d, and HMGA1. Results: HCP5 knockdown suppressed proliferation and weakened the resistance to cisplatin (DDP) of GC cells. miR-519d was down-regulated in GC cells and sponged by HCP5. HMGA1 was directly inhibited by miR-519d and its expression was up-regulated in GC cells. HCP5 exacerbated the resistance to cisplatin of GC cells in vitro by enhancing HMGA1 expression via sponging miR-519d. Conclusion: In summary, HCP5 promoted proliferation and contributed to DDP resistance in GC cells through miR-519d/HMGA1 axis.
Language	English
Publishing date	2021-01-27
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2508013-1
ISSN	1179-1322
ISSN	1179-1322
DOI	10.2147/CMAR.S289997
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Article ; Online: Osteoblast Biospecific Extraction Conjugated with HPLC Analysis for Screening Bone Regeneration Active Components from Moutan Cortex.

Yao, Fei / Chen, Wei / Gu, Weiwei / Xu, Heng / Hou, Wenyue / Liang, Guoqiang / ZhangZhu, Ruixian / Jiang, Guorong / Zhang, Lurong

Combinatorial chemistry & high throughput screening

2023

Abstract: Introduction: The function of promoting bone regeneration of Moutan Cortex (MC), a traditional Chinese medicine, has been widely known but, the effective components of MC in promoting osteoblast-mediated bone regeneration were still unclear.: ... ...

Abstract	Introduction: The function of promoting bone regeneration of Moutan Cortex (MC), a traditional Chinese medicine, has been widely known but, the effective components of MC in promoting osteoblast-mediated bone regeneration were still unclear. Objective: The method of osteoblast membrane bio-specific extraction conjugated with HPLC analysis was established to screen bone regeneration active components from MC. Methods: The fingerprints, washing eluate and desorption eluate of MC extract were analyzed by the established HPLC-DAD method. The established MC3T3-E1 cells membrane chromatography method was used for the bio-specific extraction of MC. The isolated compounds were identified by MS spectrometry. The effects and possible mechanisms of the isolated compounds were evaluated by molecular docking, ALP activity, cell viability by MTT Assay and proteins expression by Western Blot Analysis. Results: The active compound responsible for bone regeneration from MC was isolated using the established method of osteoblast membrane bio-specific extraction conjugated with HPLC analysis, and it was identified as 1,2,3,4,6-penta-O-β-galloyl-D-glucose (PGG) by MS spectrometry. It was further demonstrated through molecular docking that PGG could fit well into the functional ALP, BMP2, and Samd1 binding pocket. The proliferation of osteoblasts was promoted, the level of ALP was increased, and the protein expression of BMP2 and Smad1 was increased as shown by further pharmacological verification. Conclusion: It was concluded that PGG, the bone regeneration active compound from MC, could stimulate the proliferation of osteoblasts to promote osteoblast differentiation, and its mechanism might be related to the BMP/Smad1 pathway.
Language	English
Publishing date	2023-06-07
Publishing country	United Arab Emirates
Document type	Journal Article
ZDB-ID	2064785-2
ISSN	1875-5402 ; 1386-2073
ISSN (online)	1875-5402
ISSN	1386-2073
DOI	10.2174/1386207326666230607155913
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Zs.A 5577: Show issues

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Article ; Online: Femoral neck-shaft angle can predict the anterior capsular thickness in patients with femoracetabular impingement syndrome.

Yang, Fan / Zhang, Zhu / Zhang, Xin / Huang, Hongjie / Wang, Jianquan

Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA

2023 Volume 31, Issue 7, Page(s) 2716–2720

Abstract: Purpose: To measure the femoral neck-shaft angle (NSA) on computed tomography (CT) images in femoracetabular impingement syndrome (FAIS) patients and explore its relationship with the anterior capsular thickness (ACT).: Methods: A retrospective ... ...

Abstract	Purpose: To measure the femoral neck-shaft angle (NSA) on computed tomography (CT) images in femoracetabular impingement syndrome (FAIS) patients and explore its relationship with the anterior capsular thickness (ACT). Methods: A retrospective review of prospectively collected data from 2022 was performed. Inclusion criteria included: primary hip surgery, 18 to 55 years of age, and CT imaging of the hips. Exclusion criteria included: revision hip surgery, mild or borderline hip dysplasia, hip synovitis, and incomplete radiographs and medical records. NSA was measured on CT imaging. ACT was measured using magnetic resonance imaging (MRI). Multiple linear regression was performed to assess the association between ACT and related variables, including age, sex, body mass index (BMI), lateral center-edge angle (LCEA), alpha angle, Beighton test score (BTS), and NSA. Results: A total of 150 patients were included. The mean age, BMI, and NSA were 35.8 ± 11.2 years, 22.8 ± 3.5, and 129.4° ± 7.7°, respectively. Eighty-five (56.7%) patients were females. Multivariable regression analysis revealed that NSA (P = 0.002) and sex (P = 0.001) were significantly negatively correlated with ACT. Age, BMI, LCEA angle, alpha angle, and BTS were not correlated with ACT. Conclusions: This study confirmed that NSA significantly predicts ACT. A decrease in the NSA by 1° increases the ACT by 0.24 mm. Level of evidence: Level III.
MeSH term(s)	Female ; Humans ; Young Adult ; Adult ; Middle Aged ; Male ; Femoracetabular Impingement/diagnostic imaging ; Femoracetabular Impingement/surgery ; Femur Neck/diagnostic imaging ; Femur ; Retrospective Studies ; Hip Dislocation/surgery ; Syndrome ; Arthroscopy/methods ; Hip Joint/diagnostic imaging ; Hip Joint/surgery ; Treatment Outcome
Language	English
Publishing date	2023-02-21
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1159064-6
ISSN	1433-7347 ; 0942-2056
ISSN (online)	1433-7347
ISSN	0942-2056
DOI	10.1007/s00167-023-07347-6
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Zs.A 3742: Show issues

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Article: Therapeutic Intervention in Cancer by Isoliquiritigenin from Licorice: A Natural Antioxidant and Redox Regulator.

Zhang, Zhu / Yung, Ken Kin-Lam / Ko, Joshua Ka-Shun

Antioxidants (Basel, Switzerland)

2022 Volume 11, Issue 7

Abstract: Oxidative stress could lead to a variety of body dysfunctions, including neurodegeneration and cancer, which are closely associated with intracellular signal transducers such as reactive oxygen species (ROS). It has been suggested that ROS is the ... ...

Abstract	Oxidative stress could lead to a variety of body dysfunctions, including neurodegeneration and cancer, which are closely associated with intracellular signal transducers such as reactive oxygen species (ROS). It has been suggested that ROS is the upstream regulator of autophagy, and that it provides a negative feedback regulation to remove oxidative damage. Defects in the ROS-autophagic redox homeostasis could lead to the increased production of ROS and the accumulation of damaged organelles that in turn promote metabolic reprogramming and induce tumorigenesis. One significant characteristic of pancreatic cancer is the reprogramming of cellular energy metabolism, which facilitates the rapid growth, invasiveness, and the survival of cancer cells. Thus, the rectification of metabolic dysfunction is essential in therapeutic cancer targeting. Isoliquiritigenin (ISL) is a chalcone obtained from the plant
Language	English
Publishing date	2022-07-11
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox11071349
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