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Article: Divergent 1,2-carboallylation of terminal alkynes enabled by metallaphotoredox catalysis with switchable triplet energy transfer.

Qin, Jian / Zhang, Zhuzhu / Lu, Yi / Zhu, Shengqing / Chu, Lingling

2023 Volume 14, Issue 43, Page(s) 12143–12151

Abstract: We report a metallaphotoredox strategy for stereodivergent three-component carboallylation of terminal alkynes with allylic carbonates and alkyl trifluoroborates. This redox-neutral dual catalytic protocol utilizes commercially available organic ... ...

Abstract	We report a metallaphotoredox strategy for stereodivergent three-component carboallylation of terminal alkynes with allylic carbonates and alkyl trifluoroborates. This redox-neutral dual catalytic protocol utilizes commercially available organic photocatalyst 4CzIPN and nickel catalysts to trigger a radical addition/alkenyl-allyl coupling sequence, enabling straightforward access to functionalized 1,4-dienes in a highly chemo-, regio-selective, and stereodivergent fashion. This reaction features a broad substrate generality and a tunable triplet energy transfer control with pyrene as a simple triplet energy modulator, offering a facile synthesis of complex
Language	English
Publishing date	2023-10-13
Publishing country	England
Document type	Journal Article
ZDB-ID	2559110-1
ISSN	2041-6539 ; 2041-6520
ISSN (online)	2041-6539
ISSN	2041-6520
DOI	10.1039/d3sc04645a
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Activity of the botanical compound thymol against kiwifruit rot caused by Fusarium tricinctum and the underlying mechanisms

Wang, Weizhen / He, Linan / Zhang, Zhuzhu / Li, Wenzhi / Chen, Jia / Chen, Tingting / Long, Youhua

Pest Management Science. 2023 July, v. 79, no. 7 p.2493-2502

2023

Abstract: BACKGROUND: Kiwifruit rot is an important disease caused by different fungal pathogens, which can lead to huge economic loss in the kiwifruit industry. The aims of this study were to discover an effective botanical compound that significantly inhibits ... ...

Abstract	BACKGROUND: Kiwifruit rot is an important disease caused by different fungal pathogens, which can lead to huge economic loss in the kiwifruit industry. The aims of this study were to discover an effective botanical compound that significantly inhibits the pathogens causing kiwifruit rot, evaluate its control efficacy against this disease, and reveal the underlying mechanisms. RESULTS: A strain of Fusarium tricinctum (GF‐1), isolated from diseased kiwifruit, could cause fruit rot in both Actinidia chinensis var. chinensis and Actinidia chinensis var. deliciosa. Different botanical chemicals were used for antifungal activity test against GF‐1 and thymol was the most effective one with a 50% effective concentration (EC₅₀) of 30.98 mg L⁻¹. The minimal inhibitory concentration (MIC) of thymol against GF‐1 was 90 mg L⁻¹. Control efficacy of thymol against kiwifruit rot was evaluated and the results indicated that thymol could effectively decrease the occurrence and spread of kiwifruit rot. The mechanisms underlying the antifungal activity of thymol against F. tricinctum were investigated, and it showed that thymol could significantly damage the ultrastructure, destroy the plasma membrane integrity, and instantaneously increase energy metabolisms of F. tricinctum. Further investigations indicated that thymol could extend shelf life of kiwifruit by increasing their storability. CONCLUSION: Thymol can effectively inhibit F. tricinctum that is one of the causal agents of kiwifruit rot. Multiple modes of action are involved in the antifungal activity. The results of this study indicate that thymol can be a promising botanical fungicide to control kiwifruit rot and provide useful references for thymol application in agriculture system. © 2023 Society of Chemical Industry.
Keywords	Actinidia chinensis ; Actinidia deliciosa ; Fusarium tricinctum ; antifungal properties ; energy ; financial economics ; fungi ; fungicides ; industry ; kiwifruit ; median effective concentration ; minimum inhibitory concentration ; plant rots ; plasma membrane ; shelf life ; thymol ; ultrastructure
Language	English
Dates of publication	2023-07
Size	p. 2493-2502.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2001705-4
ISSN	1526-4998 ; 1526-498X
ISSN (online)	1526-4998
ISSN	1526-498X
DOI	10.1002/ps.7431
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Sulfur-Induced Resistance against

Zhang, Zhuzhu / Long, Youhua / Yin, Xianhui / Yang, Sen

International journal of molecular sciences

2021 Volume 22, Issue 23

Abstract: Sulfur has been previously reported to modulate plant growth and exhibit significant anti-microbial activities. However, the mechanism underlying its diverse effects on plant pathogens has not been elucidated completely. The present study conducted the ... ...

Abstract	Sulfur has been previously reported to modulate plant growth and exhibit significant anti-microbial activities. However, the mechanism underlying its diverse effects on plant pathogens has not been elucidated completely. The present study conducted the two-year field experiment of sulfur application to control kiwifruit canker from 2017 to 2018. For the first time, our study uncovered activation of plant disease resistance by salicylic acid after sulfur application in kiwifruit. The results indicated that when the sulfur concentration was 1.5-2.0 kg m
MeSH term(s)	Actinidia/drug effects ; Actinidia/growth & development ; Actinidia/immunology ; Actinidia/metabolism ; Disease Resistance/drug effects ; Disease Resistance/immunology ; Fruit/drug effects ; Fruit/growth & development ; Fruit/immunology ; Fruit/metabolism ; Plant Diseases/immunology ; Plant Diseases/microbiology ; Pseudomonas syringae/physiology ; Salicylic Acid/metabolism ; Signal Transduction ; Sulfur/pharmacology
Chemical Substances	Sulfur (70FD1KFU70) ; Salicylic Acid (O414PZ4LPZ)
Language	English
Publishing date	2021-11-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms222312710
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Activity of the botanical compound thymol against kiwifruit rot caused by Fusarium tricinctum and the underlying mechanisms.

Wang, Weizhen / He, Linan / Zhang, Zhuzhu / Li, Wenzhi / Chen, Jia / Chen, Tingting / Long, Youhua

Pest management science

2023 Volume 79, Issue 7, Page(s) 2493–2502

Abstract: Background: Kiwifruit rot is an important disease caused by different fungal pathogens, which can lead to huge economic loss in the kiwifruit industry. The aims of this study were to discover an effective botanical compound that significantly inhibits ... ...

Abstract	Background: Kiwifruit rot is an important disease caused by different fungal pathogens, which can lead to huge economic loss in the kiwifruit industry. The aims of this study were to discover an effective botanical compound that significantly inhibits the pathogens causing kiwifruit rot, evaluate its control efficacy against this disease, and reveal the underlying mechanisms. Results: A strain of Fusarium tricinctum (GF-1), isolated from diseased kiwifruit, could cause fruit rot in both Actinidia chinensis var. chinensis and Actinidia chinensis var. deliciosa. Different botanical chemicals were used for antifungal activity test against GF-1 and thymol was the most effective one with a 50% effective concentration (EC Conclusion: Thymol can effectively inhibit F. tricinctum that is one of the causal agents of kiwifruit rot. Multiple modes of action are involved in the antifungal activity. The results of this study indicate that thymol can be a promising botanical fungicide to control kiwifruit rot and provide useful references for thymol application in agriculture system. © 2023 Society of Chemical Industry.
MeSH term(s)	Thymol/pharmacology ; Antifungal Agents/pharmacology ; Antifungal Agents/metabolism ; Fruit/microbiology ; Actinidia/chemistry
Chemical Substances	Thymol (3J50XA376E) ; Antifungal Agents
Language	English
Publishing date	2023-03-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2001705-4
ISSN	1526-4998 ; 1526-498X
ISSN (online)	1526-4998
ISSN	1526-498X
DOI	10.1002/ps.7431
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Rapid detection of Pseudomonas syringae pv. actinidiae by electrochemical surface-enhanced Raman spectroscopy.

Jiang, Lingli / Luo, Longhui / Zhang, Zhuzhu / Kang, Chao / Zhao, Zhibo / Chen, Dongmei / Long, Youhua

Talanta

2023 Volume 268, Issue Pt 1, Page(s) 125336

Abstract: Bacterial cancer caused by Pseudomonas syringae pv. actinidiae (Psa) is a major threat to kiwifruit in the world, and there is still a lack of effective control measures. The field of bacterial detection needs a fast, easy-to-use and sensitive ... ...

Abstract	Bacterial cancer caused by Pseudomonas syringae pv. actinidiae (Psa) is a major threat to kiwifruit in the world, and there is still a lack of effective control measures. The field of bacterial detection needs a fast, easy-to-use and sensitive identification platform. The current bacterial identification methods are lack of time efficiency, which brings problems to many sectors of society. Surface-enhanced Raman spectroscopy (SERS) and electrochemistry (EC) have been studied as possible candidates for bacterial detection because of their high sensitivity for the detection of biomolecules. In this work, SERS, EC and electrochemical surface-enhanced Raman spectroscopy (EC-SERS) were used for the first time to study the adsorption and EC behavior of Psa on the surface of nanostructured silver electrodes. Two different Raman spectra of a single analyte were obtained, and this dual detection was realized. Silver nanoparticles with iodide and calcium ions (Ag@ICNPs) were synthesized as SERS substrates significantly enhanced the characteristic signal peaks of Psa, and the limit of detection (LOD) is as low as 1.0 × 10
MeSH term(s)	Spectrum Analysis, Raman/methods ; Pseudomonas syringae ; Metal Nanoparticles/chemistry ; Silver/chemistry ; Limit of Detection
Chemical Substances	Silver (3M4G523W1G)
Language	English
Publishing date	2023-10-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2023.125336
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Article ; Online: Base-promoted thioannulation of

Zhang, Zhu-Zhu / Sun, Cai-Ling / Zhang, Xiao-Hong / Zhang, Xing-Guo

Organic & biomolecular chemistry

2021 Volume 19, Issue 46, Page(s) 10174–10180

Abstract: A general and efficient strategy for the one-pot synthesis of isothiocoumarin-1-ones has been ... ...

Abstract	A general and efficient strategy for the one-pot synthesis of isothiocoumarin-1-ones has been developed
Language	English
Publishing date	2021-12-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2097583-1
ISSN	1477-0539 ; 1477-0520
ISSN (online)	1477-0539
ISSN	1477-0520
DOI	10.1039/d1ob02012a
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Ni-Catalyzed Reductive Fluoroalkylacylation of Alkynes for the Steroselective Synthesis of Fluoroalkylated Enones.

Zhang, Zhu-Zhu / Lei, Jia-Jia / Zhang, Xiao-Hong / Zhang, Xing-Guo / Tu, Hai-Yong

Organic letters

2022 Volume 24, Issue 33, Page(s) 6192–6196

Abstract: A Ni-catalyzed three-component reductive fluoroalkylacylation of alkynes with fluoroalkyl halides and acyl chlorides is presented. This dicarbofunctionalization provides an efficient method for the synthesis of fluoroalkyl-incorporated enones under mild ... ...

Abstract	A Ni-catalyzed three-component reductive fluoroalkylacylation of alkynes with fluoroalkyl halides and acyl chlorides is presented. This dicarbofunctionalization provides an efficient method for the synthesis of fluoroalkyl-incorporated enones under mild conditions with high yields and excellent regioselectivity and stereoselectivity.
Language	English
Publishing date	2022-08-16
Publishing country	United States
Document type	Journal Article
ISSN	1523-7052
ISSN (online)	1523-7052
DOI	10.1021/acs.orglett.2c02464
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Bearing surface defect detection based on improved convolutional neural network.

Fu, Xian / Yang, Xiao / Zhang, Ningning / Zhang, RuoGu / Zhang, Zhuzhu / Jin, Aoqun / Ye, Ruiwen / Zhang, Huiling

Mathematical biosciences and engineering : MBE

2023 Volume 20, Issue 7, Page(s) 12341–12359

Abstract: This paper addresses the issue of artificial visual inspection being overly reliant on subjective experience and the difficulty for the human eye to accurately identify dense and non-significant defects. To solve this problem, we have implemented an ... ...

Abstract	This paper addresses the issue of artificial visual inspection being overly reliant on subjective experience and the difficulty for the human eye to accurately identify dense and non-significant defects. To solve this problem, we have implemented an automatic object detection algorithm based on an improved version of YOLOv5.First, we use the K-means++ clustering algorithm to automatically calculate the Anchor of the model to reduce the effect of the close location of the initial clustering centers on the clustering of the sample data.Second, we add the Coordinate Attention (CA) attention mechanism to the model to allow the model to better capture and understand important features in the images. Then, we add a new detection layer with a downsampling multiplier of 4 to the Neck network to improve the precision of the model. Finally, we use the lightweight network MobileNetV3 instead of YOLOv5's backbone network to reduce the model detection time overhead.Our model achieves 85.87% mAP, which is 6.44% better than the YOLOv5 network, and the detection time for a single image is only 54ms, which is 50% faster than the YOLOv5 network. After testing, we have proven that our proposed algorithm can quickly and accurately detect the condition of bearing appearance defects, improving detection efficiency and reducing costs.
Language	English
Publishing date	2023-07-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2265126-3
ISSN	1551-0018 ; 1551-0018
ISSN (online)	1551-0018
ISSN	1551-0018
DOI	10.3934/mbe.2023549
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Genome-wide identification and expression patterns of the laccase gene family in response to kiwifruit bacterial canker infection.

Zhang, Zhuzhu / Long, Youhua / Yin, Xianhui / Wang, Weizhen / Li, Wenzhi / Jiang, Lingli / Chen, Xuetang / Wang, Bince / Ma, Jiling

BMC plant biology

2023 Volume 23, Issue 1, Page(s) 591

Abstract: Background: Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a destructive disease worldwide. Resistance genes that respond to Psa infection urgently need to be identified for controlling this disease. Laccase is ... ...

Abstract	Background: Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a destructive disease worldwide. Resistance genes that respond to Psa infection urgently need to be identified for controlling this disease. Laccase is mainly involved in the synthesis of lignin in the plant cell wall and plays a prominent role in plant growth and resistance to pathogen infection. However, the role of laccase in kiwifruit has not been reported, and whether laccase is pivotal in the response to Psa infection remains unclear. Results: We conducted a bioinformatics analysis to identify 55 laccase genes (AcLAC1-AcLAC55) in the kiwifruit genome. These genes were classified into five cluster groups (I-V) based on phylogenetic analysis, with cluster groups I and II having the highest number of members. Analysis of the exon-intron structure revealed that the number of exons varied from 1 to 8, with an average of 5 introns. Our evolutionary analysis indicated that fragment duplication played a key role in the expansion of kiwifruit laccase genes. Furthermore, evolutionary pressure analysis suggested that AcLAC genes were under purifying selection. We also performed a cis-acting element analysis and found that AcLAC genes contained multiple hormone (337) and stress signal (36) elements in their promoter regions. Additionally, we investigated the expression pattern of laccase genes in kiwifruit stems and leaves infected with Psa. Our findings revealed that laccase gene expression levels in the stems were higher than those in the leaves 5 days after inoculation with Psa. Notably, AcLAC2, AcLAC4, AcLAC17, AcLAC18, AcLAC26, and AcLAC42 showed significantly higher expression levels (p < 0.001) compared to the non-inoculated control (0 d), suggesting their potential role in resisting Psa infection. Moreover, our prediction indicated that 21 kiwifruit laccase genes are regulated by miRNA397, they could potentially act as negative regulators of lignin biosynthesis. Conclusions: These results are valuable for further analysis of the resistance function and molecular mechanism of laccases in kiwifruit.
MeSH term(s)	Laccase/genetics ; Phylogeny ; Lignin ; Biological Evolution ; Actinidia/genetics ; Actinidia/microbiology ; Pseudomonas syringae/physiology ; Plant Diseases/genetics ; Plant Diseases/microbiology
Chemical Substances	Laccase (EC 1.10.3.2) ; Lignin (9005-53-2)
Language	English
Publishing date	2023-11-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2059868-3
ISSN	1471-2229 ; 1471-2229
ISSN (online)	1471-2229
ISSN	1471-2229
DOI	10.1186/s12870-023-04606-z
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Article ; Online: Metabolome and Transcriptome Analysis of Sulfur-Induced Kiwifruit Stem Laccase Gene Involved in Syringyl Lignin Synthesis against Bacterial Canker.

Zhang, Zhuzhu / Long, Youhua / Yin, Xianhui / Wang, Weizhen / Li, Wenzhi / Chen, Tingting / Chen, Jia / Chen, Xuetang / Wang, Bince / Ma, Jiling

Journal of agricultural and food chemistry

2023 Volume 71, Issue 36, Page(s) 13566–13576

Abstract: Kiwifruit canker is caused ... ...

Abstract	Kiwifruit canker is caused by
MeSH term(s)	Laccase/genetics ; Lignin ; Disease Resistance ; Metabolome ; Gene Expression Profiling ; Actinidia/genetics ; Sulfur
Chemical Substances	sinapinic acid (68A28V6010) ; sinapyl alcohol (8O6NO04SMV) ; Laccase (EC 1.10.3.2) ; Lignin (9005-53-2) ; Sulfur (70FD1KFU70)
Language	English
Publishing date	2023-08-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c02653
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