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  1. Article ; Online: The Flora Compositions of Nitrogen-Fixing Bacteria and the Differential Expression of nifH Gene in Pennisetum giganteum z.x.lin Roots

    Biaosheng Lin / Jiamin Liu / Xue Zhang / Changren Weng / Zhanxi Lin

    BioMed Research International, Vol

    2021  Volume 2021

    Abstract: The flora compositions of nitrogen-fixing bacteria in roots of Pennisetum giganteum z.x.lin at different growth stages and the expression and copy number of nitrogen-fixing gene nifH were studied by Illumina Miseq second-generation sequencing technology ... ...

    Abstract The flora compositions of nitrogen-fixing bacteria in roots of Pennisetum giganteum z.x.lin at different growth stages and the expression and copy number of nitrogen-fixing gene nifH were studied by Illumina Miseq second-generation sequencing technology and qRT-PCR. The results showed that there were more than 40,000~50,000 effective sequences in 5 samples from the roots of P. giganteum, with Proteobacteria and Cyanobacteria as the dominant nitrogen-fixing bacteria based on the OTU species annotations for each sample and Bradyrhizobium as the core bacterial genera. The relative expression and quantitative change of nifH gene in roots of P. giganteum at different growth stages were consistent with the changes in the flora compositions of nitrogen-fixing microbia. Both revealed a changing trend with an initial increase and a sequential decrease, as well as changing order as jointing stage>maturation stage>tillering stage>seedling stage>dying stage. The relative expression and copy number of nifH gene were different in different growth stages, and the difference among groups basically reached a significant level (p<0.05). The relative expression and copy number of nifH gene at the jointing stage were the highest, and the 2-△△CT value was 4.43 folds higher than that at the seedling stage, with a copy number of 1.32×107/g. While at the dying stage, it was the lowest, and the 2-△△CT value was 0.67 folds, with a copy number of 0.31×107/g.
    Keywords Medicine ; R
    Subject code 580
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Ability of Three Pleurotus Species for Effective use of Giant Grass Compost

    Niyimbabazi, Obed / Nsanzinshuti, Aimable / Hatungimana, Mediatrice / Lin, Hui / Zhang, Lili / Lin, Dongmei / Zhanxi, Lin

    Journal of Horticultural Research. 2022 June 22, v. 30, no. 1

    2022  

    Abstract: Pennisetum sinese is a giant grass with a fast-growing and high rooting rate, high sugar, protein content, and high biomass yield, which causes it to be an efficient and economic energy crop of high productivity, application in phytoremediation, and ... ...

    Abstract Pennisetum sinese is a giant grass with a fast-growing and high rooting rate, high sugar, protein content, and high biomass yield, which causes it to be an efficient and economic energy crop of high productivity, application in phytoremediation, and fodder production. The composting system of this grass that is adapted to the simplest formulation is easy and economically feasible in small farms for cultivating oyster mushrooms. In this study, giant grass compost was employed as a substrate for cultivating three Pleurotus species: P. florida, P. pulmonarius, and P. ostreatus to assess their enzyme activities, growth, and yields. Lignin peroxidase (LiP) was the most active enzyme in each species, while other enzymes were differently expressed between species and developmental phases. The average mass of fruiting bodies formed on the giant grass compost was 173.4 g, 166.5 g, and 152.2 g. The biological effectivity was 82.6%, 78.6%, and 72.5% for P. pulmonarius, P. ostreatus, and P. florida, respectively. The obtained results indicate the usefulness of giant grass compost for the cultivation of the three studied Pleurotus species.
    Keywords Pennisetum ; biomass production ; composts ; economic feasibility ; energy crops ; forage ; grasses ; horticulture ; lignin peroxidase ; oysters ; phytoremediation ; protein content ; research ; sugars
    Language English
    Dates of publication 2022-0622
    Size p. 67-76.
    Publishing place Sciendo
    Document type Article
    ISSN 2353-3978
    DOI 10.2478/johr-2022-0006
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Effect of Pennisetum giganteum z.x.lin mixed nitrogen-fixing bacterial fertilizer on the growth, quality, soil fertility and bacterial community of pakchoi (Brassica chinensis L.).

    Yulei Jia / Zhen Liao / Huifang Chew / Lifang Wang / Biaosheng Lin / Chaoqian Chen / Guodong Lu / Zhanxi Lin

    PLoS ONE, Vol 15, Iss 2, p e

    2020  Volume 0228709

    Abstract: Biofertilizer plays a significant role in crop cultivation that had reduced its inorganic fertilizer use. The effects of inorganic fertilizer reduction combined with Pennisetum giganteum z.x.lin mixed nitrogen-fixing biofertilizer on the growth, quality, ...

    Abstract Biofertilizer plays a significant role in crop cultivation that had reduced its inorganic fertilizer use. The effects of inorganic fertilizer reduction combined with Pennisetum giganteum z.x.lin mixed nitrogen-fixing biofertilizer on the growth, quality, soil nutrients and diversity of the soil bacterial community in the rhizosphere soil of pakchoi were studied. The experiment composed of 6 treatments, including CK (no fertilization), DL (10% inorganic fertilizer reduction combined with Pennisetum giganteum z.x.lin mixed nitrogen-fixing biofertilizer), ZL (25% inorganic fertilizer reduction combined with Pennisetum giganteum z.x.lin mixed nitrogen-fixing biofertilizer), SL (50% inorganic fertilizer reduction combined with Pennisetum giganteum z.x.lin mixed nitrogen-fixing biofertilizer), FHF (100% inorganic fertilizer) and JZ (100% inorganic fertilizer combined with sterilized Pennisetum giganteum z.x.lin mixed nitrogen-fixing biofertilizer). Compared with conventional fertilization, the 25% reduction in chemical fertilizer applied with the Pennisetum giganteum mixed nitrogen-fixing biofertilizer resulted in higher plant height, plant weight, chlorophyll content, soluble protein content, soluble sugar content, vitamin C content, alkali hydrolyzed nitrogen content, available phosphorus content, available potassium content and organic matter content in pakchoi, and these variables increased by 11.81%, 8.54%, 7.37%, 16.88%, 17.05%, 23.70%, 24.24%, 36.56%, 21.09% and 19.72%, respectively. In addition, the 25% reduction in chemical fertilizer applied with the Pennisetum giganteum mixed nitrogen-fixing biofertilizer also had the lowest nitrate content, which was 53.86% lower than that with conventional fertilization. Different fertilizer treatments had a significant effect on the soil bacterial community structure. Compared with conventional fertilization, the coapplication of Pennisetum giganteum z.x.lin mixed nitrogen-fixing biofertilizer and inorganic fertilizer significantly increased the relative abundance of ...
    Keywords Medicine ; R ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Dietary supplement of fermented grass forage regulates growth performance, antioxidant capacity, and immune response of broiler chickens.

    Omoor, Ibrahim N A / Yankey, Richard / Shehata, Akram I / Fang, Chew H / Hui, Lin / Dongmei, Lin / Ling, Jin / Dosoky, Waleed M / Karanja, Joseph K / Dawood, Mahmoud A O / Othman, Sarah I / Allam, Ahmed A / Rudayni, Hassan A / Taha, Ayman E / Swelum, Ayman A / Tellez-Isaias, Guillermo / Zhanxi, Lin

    Poultry science

    2023  Volume 103, Issue 2, Page(s) 103323

    Abstract: The current study aimed to determine the effects of different levels of fermented Juncao grass (FG) on growth parameters, blood constituents, immunity, and antioxidative properties of broilers. A total of 240 (21-d-old) broiler chicks were randomly ... ...

    Abstract The current study aimed to determine the effects of different levels of fermented Juncao grass (FG) on growth parameters, blood constituents, immunity, and antioxidative properties of broilers. A total of 240 (21-d-old) broiler chicks were randomly distributed to four dietary treatments of sixty birds, with six replicate pens and ten birds in each. Fermented grass was added to the basal diet at four levels with 0, 5, 10, and 15% FG. The results revealed that broilers fed 5% FG had significantly higher (P < 0.05) final body weight (FBW), average daily gain (WG) and average daily feed intake (ADFI). The best conversion ratio (FCR) was recorded for broilers supplemented with 5% FG compared to the group supplemented with 15% FG (P < 0.05). Increasing FG % decreased (P < 0.05) anti-inflammatory cytokines IL-4, IL-6, and IL-10. However, FG increased (P < 0.05) proinflammatory cytokines IFN-γ, IL-1, IL-2, IL-12, and TNF-α (P < 0.05). Moreover, IgA, IgG, and IgM levels increased (P < 0.05) with increasing FG %. In addition, increasing FG % in broiler rations significantly increased (P < 0.05) serum antioxidant levels of T-AOC, GSH-PX, SOD, CAT, NO and GSH, but decreased (P < 0.05) MDA levels compared to the control group. Conclusively, fermented Juncao grass would be considered a novel herbal feed additive for improving broiler performance, immunity, antioxidant, and health status. Nevertheless, further research at the molecular level is needed to quantify the effects of these herbal components on cellular and humoral immune functions in broiler chickens.
    MeSH term(s) Animals ; Antioxidants ; Chickens/physiology ; Dietary Supplements ; Diet/veterinary ; Cytokines ; Immunity ; Animal Feed/analysis
    Chemical Substances Antioxidants ; Cytokines
    Language English
    Publishing date 2023-11-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 242586-5
    ISSN 1525-3171 ; 0032-5791
    ISSN (online) 1525-3171
    ISSN 0032-5791
    DOI 10.1016/j.psj.2023.103323
    Database MEDical Literature Analysis and Retrieval System OnLINE

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