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Article ; Online: Determination of the Absorption, Translocation, and Distribution of Imidacloprid in Wheat.

Wang, Jinhua / Cheng, Chao / Zhao, Changyu / Wang, Lanjun

Journal of visualized experiments : JoVE

2023 , Issue 194

Abstract: Neonicotinoids, a class of insecticides, are widely used because of their novel modes of action, high insecticidal activity, and strong root uptake. Imidacloprid, the most widely used insecticide worldwide, is a representative first-generation ... ...

Abstract	Neonicotinoids, a class of insecticides, are widely used because of their novel modes of action, high insecticidal activity, and strong root uptake. Imidacloprid, the most widely used insecticide worldwide, is a representative first-generation neonicotinoid and is used in pest control for crops, vegetables, and fruit trees. With such a broad application of imidacloprid, its residue in crops has attracted increasing scrutiny. In the present study, 15 wheat seedlings were placed in a culture medium containing 0.5 mg/L or 5 mg/L imidacloprid for hydroculture. The content of imidacloprid in the wheat roots and leaves was determined after 1 day, 2 days, and 3 days of hydroculture to explore the migration and distribution of imidacloprid in wheat. The results showed that imidacloprid was detected both in the roots and leaves of the wheat plant, and the content of imidacloprid in the roots was higher than that in the leaves. Furthermore, the imidacloprid concentration in the wheat increased with increasing exposure time. After 3 days of exposure, the roots and leaves of the wheat in the 0.5 mg/L treatment group contained 4.55 mg/kg ± 1.45 mg/kg and 1.30 mg/kg ± 0.08 mg/kg imidacloprid, respectively, while the roots and leaves of the 5 mg/L treatment group contained 42.5 mg/kg ± 0.62 mg/kg and 8.71 mg/kg ± 0.14 mg/kg imidacloprid, respectively. The results from the present study allow for a better understanding of pesticide residues in crops and provide a data reference for the environmental risk assessment of pesticides.
MeSH term(s)	Triticum ; Neonicotinoids ; Nitro Compounds ; Insecticides/pharmacology ; Crops, Agricultural
Chemical Substances	imidacloprid (3BN7M937V8) ; Neonicotinoids ; Nitro Compounds ; Insecticides
Language	English
Publishing date	2023-04-28
Publishing country	United States
Document type	Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/64741
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Article: Structural Characteristics and Multiple Bioactivities of

Wang, Jun / Zhao, Changyu / Li, Ping / Wang, Lei / Li, Songnan

Foods (Basel, Switzerland)

2023 Volume 12, Issue 23

Abstract: The chemical structures and functional properties of plant-based polysaccharides are critically influenced by extractive solvents, but their roles are not clear. In this study, the structural characteristics and multiple bioactivities ... ...

Abstract	The chemical structures and functional properties of plant-based polysaccharides are critically influenced by extractive solvents, but their roles are not clear. In this study, the structural characteristics and multiple bioactivities of
Language	English
Publishing date	2023-12-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12234357
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Article ; Online: Classification of mild Parkinson's disease: data augmentation of time-series gait data obtained via inertial measurement units.

Uchitomi, Hirotaka / Ming, Xianwen / Zhao, Changyu / Ogata, Taiki / Miyake, Yoshihiro

Scientific reports

2023 Volume 13, Issue 1, Page(s) 12638

Abstract: Data-augmentation methods have emerged as a viable approach for improving the state-of-the-art performances for classifying mild Parkinson's disease using deep learning with time-series data from an inertial measurement unit, considering the limited ... ...

Abstract	Data-augmentation methods have emerged as a viable approach for improving the state-of-the-art performances for classifying mild Parkinson's disease using deep learning with time-series data from an inertial measurement unit, considering the limited amount of training datasets available in the medical field. This study investigated effective data-augmentation methods to classify mild Parkinson's disease and healthy participants with deep learning using a time-series gait dataset recorded via a shank-worn inertial measurement unit. Four magnitude-domain-transformation and three time-domain-transformation data-augmentation methods, and four methods involving mixtures of the aforementioned methods were applied to a representative convolutional neural network for the classification, and their performances were compared. In terms of data-augmentation, compared with baseline classification accuracy without data-augmentation, the magnitude-domain transformation performed better than the time-domain transformation and mixed-data augmentation. In the magnitude-domain transformation, the rotation method significantly contributed to the best performance improvement, yielding accuracy and F1-score improvements of 5.5 and 5.9%, respectively. The augmented data could be varied while maintaining the features of the time-series data obtained via the sensor for detecting mild Parkinson's in gait; this data attribute may have caused the aforementioned trend. Notably, the selection of appropriate data extensions will help improve the classification performance for mild Parkinson's disease.
MeSH term(s)	Humans ; Parkinson Disease/diagnosis ; Gait ; Neural Networks, Computer ; Time Factors ; Apathy
Language	English
Publishing date	2023-08-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-39862-4
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Article: The Effect of Theaflavins on the Gut Microbiome and Metabolites in Diabetic Mice.

Wang, Jun / Qin, Yixin / Jiang, Jingjing / Shan, Hongyan / Zhao, Changyu / Li, Songnan

Foods (Basel, Switzerland)

2023 Volume 12, Issue 20

Abstract: With the development of diabetes, the gut microbiome falls into a state of dysbiosis, further affecting its progression. Theaflavins (TFs), a type of tea polyphenol derivative, show anti-diabetic properties, but their effect on the gut microbiome in ... ...

Abstract	With the development of diabetes, the gut microbiome falls into a state of dysbiosis, further affecting its progression. Theaflavins (TFs), a type of tea polyphenol derivative, show anti-diabetic properties, but their effect on the gut microbiome in diabetic mice is unclear. It is unknown whether the improvement of TFs on hyperglycemia and hyperlipidemia in diabetic mice is related to gut microbiota. Therefore, in this study, different concentrations of TFs were intragastrically administered to mice with diabetes induced by a high-fat-diet to investigate their effects on blood glucose, blood lipid, and the gut microbiome in diabetic mice, and the plausible mechanism underlying improvement in diabetes was explored from the perspective of the gut microbiome. The results showed that the TFs intervention significantly improved the hyperglycemia and hyperlipidemia of diabetic mice and affected the structure of the gut microbiome by promoting the growth of bacteria positively related to diabetes and inhibiting those negatively related to diabetes. The changes in short-chain fatty acids in mice with diabetes and functional prediction analysis suggested that TFs may affect carbohydrate metabolism and lipid metabolism by regulating the gut microbiome. These findings emphasize the ability of TFs to shape the diversity and structure of the gut microbiome in mice with diabetes induced by a high-fat diet combined with streptozotocin and have practical implications for the development of functional foods with TFs.
Language	English
Publishing date	2023-10-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12203865
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Article ; Online: Biodegradation mechanism of chlorpyrifos by Bacillus sp. H27: Degradation enzymes, products, pathways and whole genome sequencing analysis.

Liu, Changrui / Zhao, Changyu / Wang, Lanjun / Du, Xiaomin / Zhu, Lusheng / Wang, Jun / Mo Kim, Young / Wang, Jinhua

Environmental research

2023 Volume 239, Issue Pt 1, Page(s) 117315

Abstract: Chlorpyrifos (CP) is a pesticide widely used in agricultural production. However, excessive use of CP is risky for human health and the ecological environment. Microbial remediation has become a research hotspot of environmental pollution control. In ... ...

Abstract	Chlorpyrifos (CP) is a pesticide widely used in agricultural production. However, excessive use of CP is risky for human health and the ecological environment. Microbial remediation has become a research hotspot of environmental pollution control. In this study, the effective CP-degrading strain H27 (Bacillus cereus) was screened from farmland soil, and the degradation ratio was more than 80%. Then, the degradation mechanism was discussed in terms of enzymes, pathways, products and genes, and the mechanism was improved in terms of cell motility, secretory transport system and biofilm formation. The key CP-degrading enzymes were mainly intracellular enzymes (IE), and the degradation ratio reached 49.6% within 30 min. The optimal pH for IE was 7.0, and the optimal temperature was 25 °C. Using DFT and HPLC‒MS analysis, it was found that degradation mainly involved oxidation, hydrolysis and other reactions, and 3 degradation pathways and 14 products were identified, among which TCP (3,5,6-trichloro-2-pyridinol) was the main primary degradation product in addition to small molecules such as CO
MeSH term(s)	Humans ; Chlorpyrifos ; Bacillus ; Biodegradation, Environmental ; Phosphoric Monoester Hydrolases ; Whole Genome Sequencing
Chemical Substances	Chlorpyrifos (JCS58I644W) ; Phosphoric Monoester Hydrolases (EC 3.1.3.2)
Language	English
Publishing date	2023-10-05
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2023.117315
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Zs.A 726: Show issues

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Article ; Online: Rhizosphere bacteria G-H27 significantly promoted the degradation of chlorpyrifos and fosthiazate.

Zhang, Xuzhi / Gao, Yuanfei / Zhao, Changyu / Wang, Lanjun / Wen, Shengfang / Shi, Baihui / Zhu, Lusheng / Wang, Jun / Kim, Young Mo / Wang, Jinhua

The Science of the total environment

2024 Volume 917, Page(s) 169838

Abstract: Microbial remediation of polluted environments is the most promising and significant research direction in the field of bioremediation. In this study, chlorpyrifos and fosthiazate were selected as representative organophosphorus pesticides, wheat was the ...

Abstract	Microbial remediation of polluted environments is the most promising and significant research direction in the field of bioremediation. In this study, chlorpyrifos and fosthiazate were selected as representative organophosphorus pesticides, wheat was the tested plant, and fluorescently labeled degrading Bacillus cereus G-H27 were the film-forming bacteria. Exogenous strengthening technology was used to establish degrading bacterial biofilms on the root surface of wheat. The influence of root surface-degrading bacterial biofilms on the enrichment of chlorpyrifos and fosthiazate in wheat was comprehensively evaluated. First, the fluorescently-labeled degrading bacteria G-H27 was constructed, and its film-forming ability was investigated. Second, the growth- promoting characteristics and degradation ability of the bacteria G-H27 were investigated. Finally, the degradation effect of the root surface-degrading bacterial biofilm on chlorpyrifos and fosthiazate was determined. The above research provides an important material basis and method for the bioremediation of pesticide-contaminated soil.
MeSH term(s)	Chlorpyrifos/metabolism ; Pesticides/metabolism ; Organophosphorus Compounds/metabolism ; Rhizosphere ; Soil Microbiology ; Biodegradation, Environmental ; Bacillus cereus/metabolism ; Thiazolidines
Chemical Substances	Chlorpyrifos (JCS58I644W) ; Pesticides ; fosthiazate (96QJ4O095U) ; Organophosphorus Compounds ; Thiazolidines
Language	English
Publishing date	2024-01-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.169838
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Zs.A 949: Show issues

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Article ; Online: Characteristics of biological manganese oxides produced by manganese-oxidizing bacteria H38 and its removal mechanism of oxytetracycline.

Liu, Changrui / Shi, Baihui / Guo, Yuchen / Wang, Lanjun / Li, Shuhan / Zhao, Changyu / Zhu, Lusheng / Wang, Jun / Kim, Young Mo / Wang, Jinhua

Environmental pollution (Barking, Essex : 1987)

2024 Volume 345, Page(s) 123432

Abstract: Oxytetracycline (OTC) is widely used in clinical medicine and animal husbandry. Residual OTC can affect the normal life activities of microorganisms, animals, and plants and affect human health. Microbial remediation has become a research hotspot in the ... ...

Abstract	Oxytetracycline (OTC) is widely used in clinical medicine and animal husbandry. Residual OTC can affect the normal life activities of microorganisms, animals, and plants and affect human health. Microbial remediation has become a research hotspot in the environmental field. Manganese oxidizing bacteria (MnOB) exist in nature, and the biological manganese oxides (BMO) produced by them have the characteristics of high efficiency, low cost, and environmental friendliness. However, the effect and mechanism of BMO in removing OTC are still unclear. In this study, Bacillus thuringiensis strain H38 of MnOB was obtained, and the conditions for its BMO production were optimized. The optimal conditions were determined as follows: optimal temperature = 35 °C, optimal pH = 7.5, optimal Mn(Ⅱ) initial concentration = 10 mmol/L. The results show that BMO are irregular or massive, mainly containing MnCO
MeSH term(s)	Humans ; Bacteria/metabolism ; Manganese/metabolism ; Manganese Compounds/chemistry ; Naphthalenes ; Oxidation-Reduction ; Oxides/chemistry ; Oxytetracycline
Chemical Substances	Manganese (42Z2K6ZL8P) ; Manganese Compounds ; manganese oxide (64J2OA7MH3) ; Naphthalenes ; Oxides ; Oxytetracycline (X20I9EN955)
Language	English
Publishing date	2024-01-23
Publishing country	England
Document type	Journal Article
ZDB-ID	280652-6
ISSN	1873-6424 ; 0013-9327 ; 0269-7491
ISSN (online)	1873-6424
ISSN	0013-9327 ; 0269-7491
DOI	10.1016/j.envpol.2024.123432
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Zs.A 2599: Show issues

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Article ; Online: Advanced Glycation End Products, Bone Health, and Diabetes Mellitus.

Jiang, Jingjing / Zhao, Changyu / Han, Tingting / Shan, Hongyan / Cui, Guiyou / Li, Songnan / Xie, Zhongwen / Wang, Jun

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association

2022 Volume 130, Issue 10, Page(s) 671–677

Abstract: Advanced glycation end products (AGEs), the compounds resulting from the non-enzymatic glycosylation between reducing sugars and proteins, are derived from food or ... ...

Abstract	Advanced glycation end products (AGEs), the compounds resulting from the non-enzymatic glycosylation between reducing sugars and proteins, are derived from food or produced
MeSH term(s)	Humans ; Glycation End Products, Advanced/metabolism ; Bone Density ; Diabetes Mellitus/metabolism ; Bone and Bones/metabolism ; Sugars ; Receptor for Advanced Glycation End Products
Chemical Substances	Glycation End Products, Advanced ; Sugars ; Receptor for Advanced Glycation End Products
Language	English
Publishing date	2022-10-18
Publishing country	Germany
Document type	Review ; Journal Article
ZDB-ID	1225416-2
ISSN	1439-3646 ; 0947-7349
ISSN (online)	1439-3646
ISSN	0947-7349
DOI	10.1055/a-1861-2388
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Uh III Zs.91: Show issues

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Article: Under drought conditions NaCl improves the nutritional status of the xerophyte Zygophyllum xanthoxylum but not of the glycophyte Arabidopsis thaliana

Wang, Wen-Ying / Chai, Wei-Wei / Liu, Ya-Qi / Ma, Qing / Rowland, Owen / Song, Xin / Wang, Bao-Shan / Wang, Suo-Min / Zhao, Chang-Yu

Zeitschrift für Pflanzenernährung und Bodenkunde. 2019 Aug., v. 182, no. 4

2019

Abstract: Zygophyllum xanthoxylum is a salt‐accumulating xerophytic species with excellent adaptability to adverse environments. Previous studies demonstrated that Z. xanthoxylum absorbs a great quantity of Na+ as an osmoregulatory substance under arid conditions. ...

Abstract	Zygophyllum xanthoxylum is a salt‐accumulating xerophytic species with excellent adaptability to adverse environments. Previous studies demonstrated that Z. xanthoxylum absorbs a great quantity of Na+ as an osmoregulatory substance under arid conditions. To investigate the nutritional status of Z. xanthoxylum in comparison with a typical glycophyte, Arabidopsis thaliana, seedlings were exposed to NaCl (50 mM for Z. xanthoxylum and 5 mM for A. thaliana), osmotic stress (–0.5 MPa), and osmotic stress combined with the NaCl treatment. Compared to the control, NaCl treatment or osmotic stress significantly increased Na+ concentration in leaves and roots of Z. xanthoxylum, but not of A. thaliana. Under osmotic stress, the addition of NaCl significantly increased Na+ concentration in leaves and roots of Z. xanthoxylum, resulting in improved biomass and tissue water content. However, such changes were not observed in A. thaliana. Compared to the control, K+ concentrations in leaves and roots remained unchanged in Z. xanthoxylum when exposed to osmotic stress, with or without additional 50 mM NaCl. In contrast, significant reductions in shoot K+ concentrations of A. thaliana were observed under osmotic stress alone or when combined with 5 mM NaCl. Moreover, NaCl alone or when combined with osmotic stress enhanced the accumulation of N, P, Fe, Si, Ca2+, and Mg2+ in Z. xanthoxylum, but did not cause such nutritional changes in A. thaliana. Compared to the glycophyte A. thaliana, Z. xanthoxylum could accumulate Na+ and maintain the stability of nutritional status at a relatively constant level to cope with drought stress.
Keywords	Arabidopsis thaliana ; biomass ; calcium ; drought ; dry environmental conditions ; iron ; leaves ; magnesium ; nitrogen ; nutritional status ; osmotic stress ; phosphorus ; potassium ; roots ; seedlings ; silicon ; sodium ; sodium chloride ; water content ; water stress ; xerophytes ; Zygophyllum xanthoxylum
Language	English
Dates of publication	2019-08
Size	p. 597-606.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ISSN	1436-8730
DOI	10.1002/jpln.201800472
Database	NAL-Catalogue (AGRICOLA)

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Article: [Effect of Panax NotoginSeng Saponins on motor evoked potentials of spinal cord injured rats with motion function].

Yang, Hui / Zhang, Yu / Chen, Xu-Yi / Zhao, Chang-Yu / Wu, Hui-Li / Xing, Ran / Zhao, Yong-Qing

Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology

2018 Volume 32, Issue 2, Page(s) 142–145

Abstract: Objective: To investigate the effect of Panax NotoginSeng Saponins(PNS) on functional recovery of rats with spinal cord injury (SCI) after exercise.: Methods: SD normal rats were randomly divided into normal control group (Normal) and control group ( ... ...

Abstract	Objective: To investigate the effect of Panax NotoginSeng Saponins(PNS) on functional recovery of rats with spinal cord injury (SCI) after exercise. Methods: SD normal rats were randomly divided into normal control group (Normal) and control group (Sham), spinal cord injury (SCI) and spinal cord injury (SCI) + panax notoginseng saponins group (PNS) ( Results: After operation, the BBB scores of Sham group, PNS group, SCI group were lower than that of normal; MEP amplitude was lower than that of normal group; the incubation time was prolonged compared with that in normal group. In PNS group compared with that in the SCI group, BBB scores at 7,14,21 and 28 days was significantly different( Conclusions: PNS can promote the recovery of motor function after SCI in rats.
MeSH term(s)	Animals ; Disease Models, Animal ; Evoked Potentials, Motor ; Panax notoginseng/chemistry ; Rats ; Rats, Sprague-Dawley ; Saponins/pharmacology ; Spinal Cord ; Spinal Cord Injuries/drug therapy
Chemical Substances	Saponins
Language	Chinese
Publishing date	2018-07-28
Publishing country	China
Document type	Journal Article
ISSN	1000-6834
ISSN	1000-6834
DOI	10.13459/j.cnki.cjap.2016.02.012
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