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  1. Article: A novel medium for long-term primary culture of hemocytes of

    Zhao, Yaqi / Guo, Huarong

    MethodsX

    2023  Volume 11, Page(s) 102335

    Abstract: The development of a suitable shrimp cell medium is essential for achieving a long-term cell culture and finite cell line of shrimps routinely. In this study, we have successfully developed an optimal shrimp cell medium that can be used for long- ... ...

    Abstract The development of a suitable shrimp cell medium is essential for achieving a long-term cell culture and finite cell line of shrimps routinely. In this study, we have successfully developed an optimal shrimp cell medium that can be used for long-term
    Language English
    Publishing date 2023-08-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2830212-6
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2023.102335
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  2. Article: Composition, structural configuration, and antigenicity of Atlantic cod (Gadus morhua) tropomyosin

    Zhao, Yaqi / Jiang, Xingyi / Tang, Chunya / Rao, Qinchun

    Food chemistry. 2023 Jan. 15, v. 399

    2023  

    Abstract: Tropomyosin, a myofibrillar muscle protein, has been recognized as a finfish allergen. In this study, tropomyosin from Atlantic cod fillets (Gadus morhua, CTM) was purified using a two-step purification strategy (isoelectric precipitation and anion- ... ...

    Abstract Tropomyosin, a myofibrillar muscle protein, has been recognized as a finfish allergen. In this study, tropomyosin from Atlantic cod fillets (Gadus morhua, CTM) was purified using a two-step purification strategy (isoelectric precipitation and anion-exchange chromatography). CTM structural configuration in two sample matrices (impure and pure) were elaborated using different polyacrylamide gel electrophoresis (native, non-reducing, and reducing PAGE). Their corresponding immunoblots were conducted to investigate CTM antigenicity under three conditions. Overall, CTM retained solubility, integrity, and antigenicity after heat treatment. Three CTM monomeric α-type isoforms (33 kDa) were identified using two-dimensional PAGE. Under native condition, the vast majority of CTM existed in the disulfide-reduced dimeric form (66 kDa). Under non-reducing condition, sodium dodecyl sulfate (anionic surfactant) broke CTM dimers, leaving monomers and disulfide-induced tetramers. Under reducing condition, β-mercaptoethanol (thiol reducing agent) dissociated disulfide-linked CTM tetramers (132 kDa) into monomers (33 kDa). CTM retained antigenicity regardless of structural configuration under different conditions.
    Keywords Gadus morhua ; allergens ; anion exchange chromatography ; anionic surfactants ; chemical precipitation ; fish ; food chemistry ; heat treatment ; muscle protein ; polyacrylamide gel electrophoresis ; sodium dodecyl sulfate ; solubility ; thiols ; tropomyosins
    Language English
    Dates of publication 2023-0115
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2022.133966
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  3. Article ; Online: Properties of co-gel between Tenebrio Molitor larvae protein and myofibrillar protein induced by transglutaminase.

    Sun, Hailei / Zhao, Yaqi / Li, Xiaolong / Huang, Ming / Qiao, Changming / Sun, Jingxin

    Food chemistry

    2024  Volume 443, Page(s) 138609

    Abstract: This study explored the effect of adding transglutaminase (TGase) to a co-gel of Tenebrio Molitor larvae protein (TMLP) and myofibrillar protein (MP). Different concentrations of TGase (0-90 U/g) were added to the co-gel. The results showed that 60 U/g ... ...

    Abstract This study explored the effect of adding transglutaminase (TGase) to a co-gel of Tenebrio Molitor larvae protein (TMLP) and myofibrillar protein (MP). Different concentrations of TGase (0-90 U/g) were added to the co-gel. The results showed that 60 U/g TGase treatment significantly improved the gel strength and water holding capacity (WHC) by 26.51 g and 9.2 %, respectively. TGase promoted the rheological properties and accelerated the three-dimensional network structure of the co-gel. Moreover, TGase significantly increased (P < 0.05) the tyrosine residues, tryptophan residues content and hydrophobic interactions of the aliphatic groups. The chemical forces between the protein molecules changed. TGase promoted the transition of α-helix to β-sheet and free water to immobilized water, thereby improving the WHC of co-gel. The principal component analysis reflected the links among indicators. This study illustrated that TGase might be an effective strategy to improve the co-gel of TMLP and MP and emulsified meat products with insects.
    MeSH term(s) Animals ; Tenebrio/metabolism ; Larva/metabolism ; Transglutaminases/metabolism ; Muscle Proteins/chemistry ; Gels/chemistry ; Water
    Chemical Substances Transglutaminases (EC 2.3.2.13) ; Muscle Proteins ; Gels ; Water (059QF0KO0R)
    Language English
    Publishing date 2024-01-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2024.138609
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  4. Article ; Online: Sulfurized polyacrylonitrile as cathodes for advanced lithium-sulfur batteries: advances in modification strategies.

    Wu, Xiaolin / Zhao, Yaqi / Li, Hang / Zhou, Cheng / Wang, Xuanpeng / Du, Lingzhi

    Nanoscale

    2024  Volume 16, Issue 10, Page(s) 5060–5078

    Abstract: Sulfurized polyacrylonitrile (S@PAN) composites have gathered a lot of interest because of their advantages of high theoretical energy density, excellent cycling stability, and environmental friendliness. Meanwhile, their unique "covalent bonding" ... ...

    Abstract Sulfurized polyacrylonitrile (S@PAN) composites have gathered a lot of interest because of their advantages of high theoretical energy density, excellent cycling stability, and environmental friendliness. Meanwhile, their unique "covalent bonding" mechanism effectively avoids the dissolution and shuttling of polysulfides, and thus they are expected to be the most promising candidate for the cathode material in lithium-sulfur (Li-S) batteries. Over the past five years, S@PAN cathode materials have been widely studied in Li-S batteries, and it is very important to summarize the advances over time for their practical applications. This article reviews the latest progress concerning the modification of S@PAN cathode materials for improving poor electrical conductivity, low sulfur content, and sluggish reaction kinetics, and proposes possible research directions. We hope this review provides valuable insights and references for future research on Li-S batteries.
    Language English
    Publishing date 2024-03-07
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2515664-0
    ISSN 2040-3372 ; 2040-3364
    ISSN (online) 2040-3372
    ISSN 2040-3364
    DOI 10.1039/d3nr06247c
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  5. Article: Neoadjuvant therapy for non-small cell lung cancer and esophageal cancer.

    He, Yunlong / Zhao, Yaqi / Akhtar, Muhammad Luqman / Li, Yu / E, Mingyan / Nie, Huan

    American journal of cancer research

    2024  Volume 14, Issue 3, Page(s) 1258–1277

    Abstract: As the major malignant tumors in the chest, non-small cell lung cancer (NSCLC) and esophageal cancer (EC) bring huge health burden to human beings worldwide. Currently, surgery is still the mainstay for comprehensive treatment for NSCLC and EC, but the ... ...

    Abstract As the major malignant tumors in the chest, non-small cell lung cancer (NSCLC) and esophageal cancer (EC) bring huge health burden to human beings worldwide. Currently, surgery is still the mainstay for comprehensive treatment for NSCLC and EC, but the prognosis is still poor as the results of cancer recurrence and distant metastasis. Neoadjuvant therapy refers to a single or combined treatment before surgery, aiming to improve the therapeutic effects of the traditional therapies. Unfortunately, the clinical outcomes and effects of neoadjuvant therapy are still controversial due to its apparent advantages and disadvantages, and different patients may respond differentially to the same scheme of neoadjuvant therapy, which makes it urgent and necessary to develop personalized scheme of neoadjuvant therapy for different individuals. Therefore, this review summarizes the novel schemes and strategies of neoadjuvant therapy, which may help to significantly improve of life quality of patients suffering from chest-related malignancies.
    Language English
    Publishing date 2024-03-15
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2589522-9
    ISSN 2156-6976
    ISSN 2156-6976
    DOI 10.62347/TCEC1867
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  6. Article ; Online: Overview of 1,5-Selective Click Reaction of Azides with Alkynes or Their Synthetic Equivalents.

    Zhao, Yaqi / Chai, Zhengqi / Zeng, Qingrui / Zhang, Wen-Xiong

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 3

    Abstract: Nowadays, the click reaction of azides with alkynes has evolved rapidly and become one of the most efficient methods to synthesize 1,2,3-triazoles, which are an important class ... ...

    Abstract Nowadays, the click reaction of azides with alkynes has evolved rapidly and become one of the most efficient methods to synthesize 1,2,3-triazoles, which are an important class of
    Language English
    Publishing date 2023-02-01
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28031400
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    Zs.MO 81: Show issues

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  7. Article ; Online: Composition, structural configuration, and antigenicity of Atlantic cod (Gadus morhua) tropomyosin.

    Zhao, Yaqi / Jiang, Xingyi / Tang, Chunya / Rao, Qinchun

    Food chemistry

    2022  Volume 399, Page(s) 133966

    Abstract: Tropomyosin, a myofibrillar muscle protein, has been recognized as a finfish allergen. In this study, tropomyosin from Atlantic cod fillets (Gadus morhua, ... ...

    Abstract Tropomyosin, a myofibrillar muscle protein, has been recognized as a finfish allergen. In this study, tropomyosin from Atlantic cod fillets (Gadus morhua, C
    MeSH term(s) Animals ; Disulfides/metabolism ; Electrophoresis, Polyacrylamide Gel ; Fishes ; Gadus morhua/metabolism ; Tropomyosin/metabolism
    Chemical Substances Disulfides ; Tropomyosin
    Language English
    Publishing date 2022-08-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2022.133966
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  8. Article ; Online: Entrepreneurial Improvisation

    Xu Xuejiao / Zhao Yaqi / Zhang Jing

    SHS Web of Conferences, Vol 96, p

    Research Review, Integration Framework and Future Outlook

    2021  Volume 04001

    Abstract: At present, the development context of entrepreneurial improvisation research is still unclear. This article reviews and sorts out the relevant literature on entrepreneurial improvisation from the individual and organizational levels: starting from the ... ...

    Abstract At present, the development context of entrepreneurial improvisation research is still unclear. This article reviews and sorts out the relevant literature on entrepreneurial improvisation from the individual and organizational levels: starting from the connotation, constituent elements and induction mechanism, classifies and sorts out the relevant research on entrepreneurial improvisation, and proposes an integrated framework and framework based on the summary of the frontier topics of entrepreneurial improvisation. The prospect of the future is expected to provide reference for the in-depth research and practice of entrepreneurial improvisation in the future.
    Keywords entrepreneurial improvisation ; improvisation ability ; environmental turbulence ; research review ; Social Sciences ; H
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Viscosity-Responsive Electrochemiluminescence of Diphenylbenzofulvene Derivatives

    Han, Zhengang / Chai, Miaomiao / Yu, Xinyao / Wang, Jinlong / Zhao, Yaqi / Zhao, Aijuan / Lu, Xiaoquan

    Analytical Chemistry. 2023 Apr. 24, v. 95, no. 17 p.7036-7044

    2023  

    Abstract: The development of superior probes is highly desirable and valuable for viscosity measurement. Herein, we designed and reported a series of diphenylbenzofulvene (DPBF)-based organic luminophores according to the molecular regulation strategy. There are ... ...

    Abstract The development of superior probes is highly desirable and valuable for viscosity measurement. Herein, we designed and reported a series of diphenylbenzofulvene (DPBF)-based organic luminophores according to the molecular regulation strategy. There are two free-rotating phenyl groups attached to the rigid fluorene skeleton in the DPBF, enabling its unique propeller-like noncoplanar chemical structure. Benefiting from this, DPBFs could feature outstanding PL and ECL emissions with intriguing aggregation-induced characteristics. Experimental and theoretical investigations revealed that substituent, spatial structure, and molecular orbital energy profoundly affected their luminescent behaviors. It was disclosed that fluoro-substituted DPBF­(F)₂ with a smaller LUMO–HOMO band gap demonstrated the strongest ECL emission and was selected as the optimal ECL emitter. Finally, DPBF­(F)₂ featured a linear response to the viscosity and VC content with lower limits of detection (LOD) of 5.69 μcP and 38.2 nM, respectively. This study represents the first example of the ECL probe toward viscosity and will be of great significance for both ECL application and viscosity measurement.
    Keywords analytical chemistry ; chemical structure ; electrochemiluminescence ; energy ; fluorenes ; viscosity
    Language English
    Dates of publication 2023-0424
    Size p. 7036-7044.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.3c00727
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  10. Article ; Online: A comparative metabolomics analysis of phytochemcials and antioxidant activity between broccoli floret and by-products (leaves and stalks).

    Zhao, Yaqi / Zhang, Yanli / Yang, Haixia / Xu, Zhenzhen / Li, Zhansheng / Zhang, Zhanquan / Zhang, Wenyuan / Deng, Jianjun

    Food chemistry

    2024  Volume 443, Page(s) 138517

    Abstract: Leaves and stalks, which account for about 45% and 25% of broccoli biomass, respectively, are usually discarded during broccoli production, leading to the waste of green resources. In this study, the phytochemical composition and antioxidant capacity of ... ...

    Abstract Leaves and stalks, which account for about 45% and 25% of broccoli biomass, respectively, are usually discarded during broccoli production, leading to the waste of green resources. In this study, the phytochemical composition and antioxidant capacity of broccoli florets and their by-products (leaves and stalks) were comprehensively analyzed. The metabolomics identified several unique metabolites (e.g., scopoletin, Harpagoside, and sinalbin) in the leaves and stalks compared to florets. Notably, the leaves were found to be a rich source of flavonoids and coumarins, with superior antioxidant capacity. The random forest model and correlation analysis indicated that flavonoids, coumarin, and indole compounds were the important factors contributing to the antioxidant activity. Moreover, the stalks contained higher levels of carbohydrates and exhibited better antioxidant enzyme activity. Together, these results provided valuable data to support the comprehensive utilization of broccoli waste, the development of new products, and the expansion of the broccoli industry chain.
    MeSH term(s) Antioxidants/chemistry ; Brassica/chemistry ; Plant Leaves/chemistry ; Flavonoids/analysis ; Carbohydrates/analysis
    Chemical Substances Antioxidants ; Flavonoids ; Carbohydrates
    Language English
    Publishing date 2024-01-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2024.138517
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