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Article ; Online: Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure

Sonia Ndeupen / Zhen Qin / Botond Z. Igyártó

STAR Protocols, Vol 3, Iss 2, Pp 101350- (2022)

2022

Abstract: Summary: We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how ...

Abstract	Summary: We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal, intramuscular, and intranasal routes, generating single-cell suspensions from the inoculated and target organs for downstream analyses.For complete details on the use and execution of this protocol, please refer to Ndeupen et al. (2021) and (2022).
Keywords	Health Sciences ; Immunology ; Science (General) ; Q1-390
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Water Conservancy System and Urban Layout of Yinxu, the Capital City of Shang

Yin Wang / Zhen Qin / Weidong Hou / Michael Storozum

Land, Vol 11, Iss 7, p

A GIS Approach

2022 Volume 986

Abstract: Archaeological work began in 1928 at Yinxu (also known as Yin Ruins), the site of the capital of the late Shang Dynasty, and the continuous excavations have yielded the discovery of numerous remains and relics. The late Shang Dynasty was the zenith of ... ...

Abstract	Archaeological work began in 1928 at Yinxu (also known as Yin Ruins), the site of the capital of the late Shang Dynasty, and the continuous excavations have yielded the discovery of numerous remains and relics. The late Shang Dynasty was the zenith of China’s Bronze Age, and research on various aspects of Yinxu has gained attention among Chinese and international archaeologists. The layout of Yinxu, especially the water conservancy systems, has become a popular subject of research in recent years. Nevertheless, quantitative research is lacking in the existing literature. Using geographic information systems (GIS) allows such research to be carried out. This study used the hydrology and density analysis modules of ArcGIS software to study the water system and urban layout of Yinxu quantitatively. The results show that the water conservancy system altered the surface runoff pattern of the city and effectively harnessed limited local water resources. The system, centered on artificial channels, was built during the Second Phase of Yinxu, largely because of climate change and the city’s expansion. Its construction, in turn, profoundly affected the layout of the city. Dwellings and handicraft workshops clustered around areas with abundant water resources; together with the Huan River, the large-scale water conservancy facilities acted as partitions that practically isolated the “central zone”, creating a wall-less defense system that differs from other capital cities in the Bronze Age of China.
Keywords	Yinxu ; GIS ; ancient capitals of China ; water conservancy system ; paleoenvironment ; Agriculture ; S
Subject code	930
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Event Detection in Continuous Video: An Inference in Point Process Approach.

Zhen Qin / Shelton, Christian R

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society

2017 Volume 26, Issue 12, Page(s) 5680–5691

Abstract: We propose a novel approach toward event detection in real-world continuous video sequences. The method: 1) is able to model arbitrary-order non-Markovian dependences in videos to mitigate local visual ambiguities; 2) conducts simultaneous event ... ...

Abstract	We propose a novel approach toward event detection in real-world continuous video sequences. The method: 1) is able to model arbitrary-order non-Markovian dependences in videos to mitigate local visual ambiguities; 2) conducts simultaneous event segmentation and labeling; and 3) is time-window free. The idea is to represent a video as an event stream of both high-level semantic events and low-level video observations. In training, we learn a point process model called a piecewise-constant conditional intensity model (PCIM) that is able to capture complex non-Markovian dependences in the event streams. In testing, event detection can be modeled as the inference of high-level semantic events, given low-level image observations. We develop the first inference algorithm for PCIM and show it samples exactly from the posterior distribution. We then evaluate the video event detection task on real-world video sequences. Our model not only provides competitive results on the video event segmentation and labeling task, but also provides benefits, including being interpretable and efficient.
Language	English
Publishing date	2017-08-25
Publishing country	United States
Document type	Journal Article
ISSN	1941-0042
ISSN (online)	1941-0042
DOI	10.1109/TIP.2017.2745209
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Pre-exposure to mRNA-LNP inhibits adaptive immune responses and alters innate immune fitness in an inheritable fashion.

Zhen Qin / Aurélie Bouteau / Christopher Herbst / Botond Z Igyártó

PLoS Pathogens, Vol 18, Iss 9, p e

2022 Volume 1010830

Abstract: Hundreds of millions of SARS-CoV-2 mRNA-LNP vaccine doses have already been administered to humans. However, we lack a comprehensive understanding of the immune effects of this platform. The mRNA-LNP-based SARS-CoV-2 vaccine is highly inflammatory, and ... ...

Abstract	Hundreds of millions of SARS-CoV-2 mRNA-LNP vaccine doses have already been administered to humans. However, we lack a comprehensive understanding of the immune effects of this platform. The mRNA-LNP-based SARS-CoV-2 vaccine is highly inflammatory, and its synthetic ionizable lipid component responsible for the induction of inflammation has a long in vivo half-life. Since chronic inflammation can lead to immune exhaustion and non-responsiveness, we sought to determine the effects of pre-exposure to the mRNA-LNP on adaptive immune responses and innate immune fitness. We found that pre-exposure to mRNA-LNPs or LNP alone led to long-term inhibition of the adaptive immune response, which could be overcome using standard adjuvants. On the other hand, we report that after pre-exposure to mRNA-LNPs, the resistance of mice to heterologous infections with influenza virus increased while resistance to Candida albicans decreased. The diminished resistance to Candida albicans correlated with a general decrease in blood neutrophil percentages. Interestingly, mice pre-exposed to the mRNA-LNP platform can pass down the acquired immune traits to their offspring, providing better protection against influenza. In summary, the mRNA-LNP vaccine platform induces long-term unexpected immunological changes affecting both adaptive immune responses and heterologous protection against infections. Thus, our studies highlight the need for more research to determine this platform's true impact on human health.
Keywords	Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2022-09-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The effect of overnight consolidation in the perceptual learning of non-native tonal contrasts.

Zhen Qin / Caicai Zhang

PLoS ONE, Vol 14, Iss 12, p e

2019 Volume 0221498

Abstract: Sleep-mediated overnight consolidation has been found to facilitate perceptual learning by promoting learners' generalization across talkers in their perception of novel segmental categories. Lexical tone is characterized by high variability across ... ...

Abstract	Sleep-mediated overnight consolidation has been found to facilitate perceptual learning by promoting learners' generalization across talkers in their perception of novel segmental categories. Lexical tone is characterized by high variability across talkers, and displays dynamic change over time. For this reason, it remains unclear whether a similar effect of overnight consolidation would be found for perceptual learning of novel tonal contrasts. Thus, this study aims to examine whether overnight consolidation facilitates talker-independent learning of lexical tones in the identification and discrimination of novel Cantonese level tones by Mandarin listeners. Two groups of Mandarin listeners were perceptually trained either in the morning or in the evening. Listeners were trained in a tone identification (ID) task with feedback using stimuli produced by a trained talker. Their post-training changes and generalization to a novel talker were then tested in the ID and AX discrimination tasks using stimuli produced by trained and untrained talkers in three posttests following training: immediately after training, 12-hour delay, and 24-hour delay. While the evening group slept between the first and second posttests, the morning group did not. The accuracy rates in the ID task showed that the evening group showed an improved trend, predicted by their individual sleep time, in identifying the level tones produced by both the trained and untrained talkers; in contrast, the morning group showed a declining trend. The d-prime scores in the AX discrimination task did not show different patterns between the two groups. The finding of sleep-related identification changes over time suggests that overnight consolidation might have facilitated tone learning of stimuli produced by the novel talker and eventually facilitated the formation of a more talker-independent representation of novel tone categories in long-term memory. The results are discussed in light of the features of lexical tones to shed light on the mechanism of ...
Keywords	Medicine ; R ; Science ; Q
Subject code	150
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Free-Standing rGO-CNT Nanocomposites with Excellent Rate Capability and Cycling Stability for Na 2 SO 4 Aqueous Electrolyte Supercapacitors

Xiaohan Du / Zhen Qin / Zijiong Li

Nanomaterials, Vol 11, Iss 1420, p

2021 Volume 1420

Abstract: Facing the increasing demand for various renewable energy storage devices and wearable and portable energy storage systems, the research on electrode materials with low costs and high energy densities has attracted great attention. Herein, free-standing ... ...

Abstract	Facing the increasing demand for various renewable energy storage devices and wearable and portable energy storage systems, the research on electrode materials with low costs and high energy densities has attracted great attention. Herein, free-standing rGO-CNT nanocomposites have been successfully synthesized by a facile hydrothermal method, in which the hierarchical porous network nanostructure is synergistically assembled by rGO nanosheets and CNT with interlaced network distribution. The rGO-CNT composite electrodes with synergistic enhancement of rGO and CNT exhibit high specific capacitance, excellent rate capability, exceptional conductivity and outstanding long-term cycling stability, especially for the optimal rGO-CNT 30 electrode. Applied to a symmetric supercapacitor systems (SSS) assembled with an rGO-CNT 30 electrode and with 1 M Na 2 SO 4 aqueous solution as the electrolyte, the SSS possesses a high energy density of 12.29 W h kg −1 and an outstanding cycling stability, with 91.42% of initial specific capacitance after 18,000 cycles. Results from these electrochemical properties suggest that the rGO-CNT 30 nanocomposite electrode is a promising candidate for the development of flexible and lightweight high-performance supercapacitors.
Keywords	rGO-CNT nanocomposite ; synergistic enhancement effect ; excellent electrochemical properties ; supercapacitors ; Chemistry ; QD1-999
Subject code	620
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Time-Series InSAR Deformation Monitoring of High Fill Characteristic Canal of South–North Water Diversion Project in China

Hui Liu / Wenfei Zhao / Zhen Qin / Tiesheng Wang / Geshuang Li / Mengyuan Zhu

Applied Sciences, Vol 13, Iss 6415, p

2023 Volume 6415

Abstract: The Middle Route of the South–North Water Diversion Project has changed the water resources pattern in China. As advanced equipment for the country, it is responsible for the water supply “lifeline” of Beijing, Tianjin, Hebei, Henan, etc. Ensuring its ... ...

Abstract	The Middle Route of the South–North Water Diversion Project has changed the water resources pattern in China. As advanced equipment for the country, it is responsible for the water supply “lifeline” of Beijing, Tianjin, Hebei, Henan, etc. Ensuring its safe operation is a top priority to promote social stability and coordinated economic development between the North and the South. Used persistent scatterer interferometric synthetic aperture radar (PS-InSAR) technology to monitor the deformation of the high fill characteristic canal in Wenzhuang Village, Ye County, during the period from October 2016 to June 2017 for the South–North Water Diversion Project showed that there was significant deformation on the 1 km-long slope of the east bank of the canal, with the maximum deformation volume reaching 36 mm. Through the comparison and verification with the second order leveling data, there are more than 87% of the root mean square error of both less than ±2 mm. The correlation coefficient is 0.96, and the two were highly consistent in deformation trends and values. Through the vertical and cross-sectional analysis of the canal’s east bank and four key monitoring sections, it was found that the east bank of the canal presents overall uneven subsidence, and the closer the canal is to the water, the greater the canal deformation, and vice versa. Further comparison of the PS-InSAR deformation results of the canal from October 2016 to February 2018 proves that this technology cannot only monitor the subsidence range and rate of the South–North Water Diversion canal but also accurately identify the subsidence sequence of the east and west banks. It can provide reliable technical support for the safety monitoring and disaster prevention of the South–North Water Diversion canal characterized by high fill and deep excavation.
Keywords	South–North Water Diversion Project ; time-series InSAR ; deformation monitoring ; high fill ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	551
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A rare early-onset neonatal case of Birk-Barel syndrome presenting severe obstructive sleep apnea

Qian Zhang / Zhen Qin / Ruolan Hu / Yifei Li / Fan Yang / Jinrong Li

Frontiers in Medicine, Vol

a case report

2023 Volume 10

Abstract: BackgroundBirk-Barel syndrome, also known as KCNK9 imprinting syndrome, is a rare fertility disorder. And the main clinical manifestations include congenital hypotonic, craniofacial malformation, developmental delay, and intellectual disability. ... ...

Abstract	BackgroundBirk-Barel syndrome, also known as KCNK9 imprinting syndrome, is a rare fertility disorder. And the main clinical manifestations include congenital hypotonic, craniofacial malformation, developmental delay, and intellectual disability. Generally, such patients could be diagnosed beyond the infant period. Moreover, the delayed diagnosis might lead to a poor prognosis of rehabilitation therapy. However, neonatal obstructive sleep apnea (OSA) was seldom reported in Birk-Barel syndrome. Here, we reported a severe neonatal OSA case induced by Birk-Barel syndrome, resulting in an early diagnosis with improved outcomes by integrative management.Case presentationThe proband was a neonate presenting with recurrent severe OSA, with craniofacial deformity and congenital muscle hypotonia. Bronchoscopy examinations indicated a negative finding of pharyngeal and bronchus stenosis, while laryngomalacia had been observed. Whole exon sequencing demonstrated a c. 710C>A heterozygous variant resulting in a change of amino acid (p.A237D). This variant resulted in a change of amino acid sequence, affected protein features and changed splice site leading to a structural deformation in KCNK9 protein. This p.A237D variant also affected the crystal structure on the p.G129 site. Additionally, we used the mSCM tool to measure the free energy changes between wild-type and mutant protein, which indicated highly destabilizing (−2.622 kcal/mol).ConclusionThis case report expands the understanding of Birk-Barel syndrome and indicates that OSA could serve as the on-set manifestation of Birk-Barel syndrome. This case emphasized genetic variants which were associated with severe neonatal OSA. Adequate WES assessment promotes early intervention and improves the prognosis of neurological disorders in young children.
Keywords	KCNK9 ; OSA ; Birk-Barel syndrome ; TASK3 channel ; case report ; Medicine (General) ; R5-920
Subject code	610 ; 572
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Construction and pattern optimization of ecological network in Zhengzhou metropolitan area.

Huo, Jin-Geng / Shi, Zhen-Qin / Zhu, Wen-Bo / Xue, Hua / Chen, Xin

Ying yong sheng tai xue bao = The journal of applied ecology

2023 Volume 34, Issue 3, Page(s) 742–750

Abstract: The rapid urbanization process has led to the increasingly prominent problems of ecological environment protection and optimization. The construction of ecological network system, planning to guide ecological values, and efficiently utilizing landscape ... ...

Title translation	郑州大都市区生态网络构建及格局优化.
Abstract	The rapid urbanization process has led to the increasingly prominent problems of ecological environment protection and optimization. The construction of ecological network system, planning to guide ecological values, and efficiently utilizing landscape effects are of great significance for regional regulation of ecological space and promotion of local sustainable development. Taking Zhengzhou metropolitan area as an example, based on land use data at eight time points from 1980 to 2020, using ArcGIS, Guidos Toolbox, Conefor and other tools, we outlined the ecological network with a high structural integrity in the study area. We used future land use simulation to predict future land pattern, morphological spatial pattern analysis to identify landscape elements, minimum cumulative resistance to construct comprehensive resistance surface, gravity model to calculate ecological gravity, hydrologic analysis to create resistance paths, and network structure evaluation,
MeSH term(s)	Conservation of Natural Resources ; Ecosystem ; Urbanization ; Sustainable Development ; Rivers ; China
Language	English
Publishing date	2023-04-23
Publishing country	China
Document type	Journal Article
ZDB-ID	2881809-X
ISSN	1001-9332
ISSN	1001-9332
DOI	10.13287/j.1001-9332.202302.025
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Article ; Online: Mobile Money Fraud Prediction—A Cross-Case Analysis on the Efficiency of Support Vector Machines, Gradient Boosted Decision Trees, and Naïve Bayes Algorithms

Francis Effirim Botchey / Zhen Qin / Kwesi Hughes-Lartey

Information, Vol 11, Iss 383, p

2020 Volume 383

Abstract: The onset of COVID-19 has re-emphasized the importance of FinTech especially in developing countries as the major powers of the world are already enjoying the advantages that come with the adoption of FinTech. Handling of physical cash has been ... ...

Abstract	The onset of COVID-19 has re-emphasized the importance of FinTech especially in developing countries as the major powers of the world are already enjoying the advantages that come with the adoption of FinTech. Handling of physical cash has been established as a means of transmitting the novel corona virus. Again, research has established that, been unbanked raises the potential of sinking one into abject poverty. Over the years, developing countries have been piloting the various forms of FinTech, but the very one that has come to stay is the Mobile Money Transactions (MMT). As mobile money transactions attempt to gain a foothold, it faces several problems, the most important of them is mobile money fraud. This paper seeks to provide a solution to this problem by looking at machine learning algorithms based on support vector machines (kernel-based), gradient boosted decision tree (tree-based) and Naïve Bayes (probabilistic based) algorithms, taking into consideration the imbalanced nature of the dataset. Our experiments showed that the use of gradient boosted decision tree holds a great potential in combating the problem of mobile money fraud as it was able to produce near perfect results.
Keywords	FinTech ; mobile money transaction ; fraud ; machine learning ; Information technology ; T58.5-58.64 ; covid19
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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