LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 3 of total 3

Search options

  1. Article: Characterization of size-resolved effective density of atmospheric particles in an urban atmosphere in Southern China.

    Xie, Tingting / Cao, Liming / Zheng, Jinyi / Xuan, Peng / Huang, Xiaofeng

    Journal of environmental sciences (China)

    2023  Volume 141, Page(s) 194–204

    Abstract: Effective density ( ... ...

    Abstract Effective density (ρ
    MeSH term(s) Air Pollutants/analysis ; Particulate Matter/analysis ; Particle Size ; Aerosols/analysis ; Atmosphere ; China ; Environmental Monitoring/methods
    Chemical Substances Air Pollutants ; Particulate Matter ; Aerosols
    Language English
    Publishing date 2023-09-24
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1092300-7
    ISSN 1878-7320 ; 1001-0742
    ISSN (online) 1878-7320
    ISSN 1001-0742
    DOI 10.1016/j.jes.2023.09.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 7630: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: The nuclear localization signal sequence of porcine circovirus type 2 ORF2 enhances intracellular delivery of plasmid DNA.

    Chen, Hsu-Chung Gabriel / Chiou, Siao-Ting / Zheng, Jin-Yi / Yang, Shu-Hsiang / Lai, Shiow-Suey / Kuo, Tsun-Yung

    Archives of virology

    2011  Volume 156, Issue 5, Page(s) 803–815

    Abstract: The phospholipid bilayer of the cell membrane is a natural barrier that prevents large molecules from entering the cell. Cationic liposomes are commonly used for transfection of plasmid DNA but they have high cost and toxicity. Many reports have shown ... ...

    Abstract The phospholipid bilayer of the cell membrane is a natural barrier that prevents large molecules from entering the cell. Cationic liposomes are commonly used for transfection of plasmid DNA but they have high cost and toxicity. Many reports have shown that cell-penetrating peptides (CPP) are able to translocate across the cell membrane efficiently. The VP22 peptide of herpes simplex virus (HSV) was synthesized as a CPP. Two fusion protein candidates, containing binding/condensing protein (VP22-TmHU) and porcine circovirus type 2 nuclear localization signal (VP22-TmHU-PCV2.NLS), were constructed and expressed in E. coli in an attempt to improve delivery of plasmid DNA (pDNA). Firstly, as shown by the electrophoretic mobility shift assay (EMSA), VP22-TmHU (VT) and VP22-TmHU-PCV2.NLS (VTN) were able to bind to pDNA (pEGFP-N1) effectively. Secondly, intracellular transport of pEGFP-N1 was observed by fluorescence microscopy and quantified by flow cytometry after transfection. VTN was successful in delivering pEGFP-N1 intracellularly but VT was not. Thirdly, two protein candidates were combined with Lipofectamine, and both VT and VTN enhanced the transfection rate to 65%, compared to 25% with Lipofectamine alone. Lastly, mice were injected intramuscularly with PBS, pcDNA3-ORF2, pcDNA3-ORF2 plus Lipofectamine, pcDNA3-ORF2 plus VT, pcDNA3-ORF2 plus VT plus Lipofectamine, pcDNA3-ORF2 plus VTN, and pcDNA3-ORF2 plus VTN plus Lipofectamine. The highest level of antibodies raised against PCV2 ORF2 Cap protein was detected with pcDNA3-ORF2 plus VTN. Contrary to the in vitro results, VTN delivered pDNA effectively in vivo without Lipofectamine. In summary, the nuclear localization signal sequence of porcine circovirus type 2 ORF2 can enhance intracellular delivery of pDNA.
    MeSH term(s) Animals ; CHO Cells ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Circovirus/genetics ; Cricetinae ; Cricetulus ; DNA/administration & dosage ; DNA/metabolism ; Electrophoretic Mobility Shift Assay ; Escherichia coli/genetics ; Flow Cytometry ; Green Fluorescent Proteins/analysis ; Mice ; Mice, Inbred BALB C ; Microscopy, Fluorescence ; Nuclear Localization Signals/genetics ; Nuclear Localization Signals/metabolism ; Plasmids/administration & dosage ; Plasmids/metabolism ; Protein Binding ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Simplexvirus/genetics ; Staining and Labeling/methods ; Vaccines, DNA/administration & dosage ; Vaccines, DNA/immunology ; Viral Proteins/genetics ; Viral Proteins/metabolism
    Chemical Substances Carrier Proteins ; Nuclear Localization Signals ; Recombinant Fusion Proteins ; Vaccines, DNA ; Viral Proteins ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9) ; DNA (9007-49-2)
    Language English
    Publishing date 2011-05
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-011-0920-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.110: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: The nuclear localization signal sequence of porcine circovirus type 2 ORF2 enhances intracellular delivery of plasmid DNA

    Chen, Hsu-Chung Gabriel / Chiou, Siao-Ting / Zheng, Jin-Yi / Yang, Shu-Hsiang / Lai, Shiow-Suey / Kuo, Tsun-Yung

    Archives of virology. 2011 May, v. 156, no. 5

    2011  

    Abstract: The phospholipid bilayer of the cell membrane is a natural barrier that prevents large molecules from entering the cell. Cationic liposomes are commonly used for transfection of plasmid DNA but they have high cost and toxicity. Many reports have shown ... ...

    Abstract The phospholipid bilayer of the cell membrane is a natural barrier that prevents large molecules from entering the cell. Cationic liposomes are commonly used for transfection of plasmid DNA but they have high cost and toxicity. Many reports have shown that cell-penetrating peptides (CPP) are able to translocate across the cell membrane efficiently. The VP22 peptide of herpes simplex virus (HSV) was synthesized as a CPP. Two fusion protein candidates, containing binding/condensing protein (VP22-TmHU) and porcine circovirus type 2 nuclear localization signal (VP22-TmHU-PCV2.NLS), were constructed and expressed in E. coli in an attempt to improve delivery of plasmid DNA (pDNA). Firstly, as shown by the electrophoretic mobility shift assay (EMSA), VP22-TmHU (VT) and VP22-TmHU-PCV2.NLS (VTN) were able to bind to pDNA (pEGFP-N1) effectively. Secondly, intracellular transport of pEGFP-N1 was observed by fluorescence microscopy and quantified by flow cytometry after transfection. VTN was successful in delivering pEGFP-N1 intracellularly but VT was not. Thirdly, two protein candidates were combined with Lipofectamine, and both VT and VTN enhanced the transfection rate to 65%, compared to 25% with Lipofectamine alone. Lastly, mice were injected intramuscularly with PBS, pcDNA3-ORF2, pcDNA3-ORF2 plus Lipofectamine, pcDNA3-ORF2 plus VT, pcDNA3-ORF2 plus VT plus Lipofectamine, pcDNA3-ORF2 plus VTN, and pcDNA3-ORF2 plus VTN plus Lipofectamine. The highest level of antibodies raised against PCV2 ORF2 Cap protein was detected with pcDNA3-ORF2 plus VTN. Contrary to the in vitro results, VTN delivered pDNA effectively in vivo without Lipofectamine. In summary, the nuclear localization signal sequence of porcine circovirus type 2 ORF2 can enhance intracellular delivery of pDNA.
    Keywords Escherichia coli ; Porcine circovirus-2 ; antibodies ; cell membranes ; electrophoresis ; flow cytometry ; fluorescence microscopy ; herpes simplex ; mice ; nuclear localization signals ; peptides ; phospholipids ; plasmids ; proteins ; toxicity ; transfection ; viruses
    Language English
    Dates of publication 2011-05
    Size p. 803-815.
    Publisher Springer Vienna
    Publishing place Vienna
    Document type Article
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-011-0920-4
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 70/128: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top