Article: Gene silencing in androgen-responsive prostate cancer cells from the tissue-specific prostate-specific antigen promoter.
2004 Volume 64, Issue 21, Page(s) 7661–7663
Abstract: The success of gene therapy using a RNA interference approach relies on small interfering RNA (siRNA) expression from a highly tissue-specific RNA polymerase II promoter rather than from ubiquitous RNA polymerase III. Accordingly, we have developed a ... ...
Abstract | The success of gene therapy using a RNA interference approach relies on small interfering RNA (siRNA) expression from a highly tissue-specific RNA polymerase II promoter rather than from ubiquitous RNA polymerase III. Accordingly, we have developed a prostate-specific vector that expresses siRNAs from the human prostate-specific antigen promoter, a RNA polymerase II promoter. Our data demonstrate androgen-dependent and tissue-specific siRNA-mediated gene silencing in the androgen-responsive prostate cancer cell line, LNCaP. The biological significance was evidenced by altered apoptotic activity through the inhibition of the apoptosis-related regulatory gene. These results demonstrate that siRNA-mediated gene silencing from a tissue-specific RNA polymerase II promoter could be a potential tool for tissue-specific gene therapy. |
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MeSH term(s) | Androgens/pharmacology ; Base Sequence ; Cell Line, Tumor ; Gene Silencing ; Humans ; In Situ Nick-End Labeling ; JNK Mitogen-Activated Protein Kinases/physiology ; Male ; Molecular Sequence Data ; Promoter Regions, Genetic ; Prostate-Specific Antigen/genetics ; Prostatic Neoplasms/genetics ; RNA, Small Interfering/pharmacology |
Chemical Substances | Androgens ; RNA, Small Interfering ; JNK Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; Prostate-Specific Antigen (EC 3.4.21.77) |
Language | English |
Publishing date | 2004-11-01 |
Publishing country | United States |
Document type | Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. |
ZDB-ID | 1432-1 |
ISSN | 1538-7445 ; 0008-5472 |
ISSN (online) | 1538-7445 |
ISSN | 0008-5472 |
DOI | 10.1158/0008-5472.CAN-04-1751 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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