LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 12

Search options

  1. Article ; Online: Computer-Visualized Sound Parameter Analysis Method and Its Application in Vocal Music Teaching

    Zhenming Yu

    Advances in Multimedia, Vol

    2022  Volume 2022

    Abstract: In order to improve the quality of vocal music teaching, this paper applies the computer visualization sound parameter analysis method to vocal music teaching and discusses the scheme of parametric coding. Moreover, this paper adopts the transient signal ...

    Abstract In order to improve the quality of vocal music teaching, this paper applies the computer visualization sound parameter analysis method to vocal music teaching and discusses the scheme of parametric coding. Moreover, this paper adopts the transient signal detection mechanism to divide the signal. For frames that have detected a shock signal, frequency-domain parametric differential predictive coding can be used like temporal noise shaping (TNS) techniques. In addition, based on the short-term periodicity and short-term stationarity of speech signals, an analytical synthesis model based on harmonic decomposition is proposed. Through the simulation data analysis, it can be seen that the computer visualization sound parameter analysis method proposed in this paper has a very good application effect in vocal music teaching and can improve the quality of vocal music teaching.
    Keywords Electronic computers. Computer science ; QA75.5-76.95
    Subject code 780
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  2. Article ; Online: Physiological and proteomic analyses reveal the effects of exogenous nitrogen in diminishing Cd detoxification in Acacia auriculiformis

    Guihua Zhang / Zhenming Yu / Lingling Zhang / Bo Yao / Xianzhen Luo / Meijuan Xiao / Dazhi Wen

    Ecotoxicology and Environmental Safety, Vol 229, Iss , Pp 113057- (2022)

    2022  

    Abstract: Cadmium (Cd) has toxic effects on plants. Nitrogen (N), an essential element, is critical for plant growth, development and stress response. However, their combined effects on woody plants, especially in N-fixing tree species is still poorly understood. ... ...

    Abstract Cadmium (Cd) has toxic effects on plants. Nitrogen (N), an essential element, is critical for plant growth, development and stress response. However, their combined effects on woody plants, especially in N-fixing tree species is still poorly understood. Our previous study revealed that the fast-growing Acacia auriculiformis showed strong Cd tolerance but the underlying mechanisms was not clear, which constrained its use in mine land reclamation. Herein, we investigated the physiological and proteomic changes in A. auriculiformis leaves to reveal the mechanisms of Cd tolerance and toxicity without N fertilizer (treatment Cd) and with excess N fertilizer (treatment CdN). Results showed that Cd tolerance in A. auriculiformis was closely associated with the coordinated gas exchange and antioxidant defense reactions under Cd treatment alone. Exogenous excessive N, however, inhibited plant growth, increased Cd concentrations, and weaken photosynthetic performance, thus, aggregated the toxicity under Cd stress. Furthermore, the aggregated Cd toxicity was attributed to the depression in the abundance of proteins, as well as their corresponding genes, involved in photosynthesis, energy metabolism (oxidative phosphorylation, carbon metabolism, etc.), defense and stress response (antioxidants, flavonoids, etc.), plant hormone signal transduction (MAPK, STN, etc.), and ABC transporters. Collectively, this study unveils a previously unknown physiological and proteomic network that explains N diminishes Cd detoxification in A. auriculiformis. It may be counterproductive to apply N fertilizer to fast-growing, N-fixing trees planted for phytoremediation of Cd-contaminated soils.
    Keywords Cadmium ; Nitrogen ; Proteomics ; Photosynthesis ; Antioxidant defense ; N-fixing tree species ; Environmental pollution ; TD172-193.5 ; Environmental sciences ; GE1-350
    Subject code 580
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  3. Article ; Online: Genome-Wide Identification and Expression Profile of TPS Gene Family in Dendrobium officinale and the Role of DoTPS10 in Linalool Biosynthesis

    Zhenming Yu / Conghui Zhao / Guihua Zhang / Jaime A. Teixeira da Silva / Jun Duan

    International Journal of Molecular Sciences, Vol 21, Iss 5419, p

    2020  Volume 5419

    Abstract: Terpene synthase (TPS) is a critical enzyme responsible for the biosynthesis of terpenes, which possess diverse roles in plant growth and development. Although many terpenes have been reported in orchids, limited information is available regarding the ... ...

    Abstract Terpene synthase (TPS) is a critical enzyme responsible for the biosynthesis of terpenes, which possess diverse roles in plant growth and development. Although many terpenes have been reported in orchids, limited information is available regarding the genome-wide identification and characterization of the TPS family in the orchid, Dendrobium officinale . By integrating the D. officinale genome and transcriptional data, 34 TPS genes were found in D. officinale . These were divided into four subfamilies (TPS-a, TPS-b, TPS-c, and TPS-e/f). Distinct tempospatial expression profiles of DoTPS genes were observed in 10 organs of D. officinale . Most DoTPS genes were predominantly expressed in flowers, followed by roots and stems. Expression of the majority of DoTPS genes was enhanced following exposure to cold and osmotic stresses. Recombinant DoTPS10 protein, located in chloroplasts, uniquely converted geranyl diphosphate to linalool in vitro. The DoTPS10 gene, which resulted in linalool formation, was highly expressed during all flower developmental stages. Methyl jasmonate significantly up-regulated DoTPS10 expression and linalool accumulation. These results simultaneously provide valuable insight into understanding the roles of the TPS family and lay a basis for further studies on the regulation of terpenoid biosynthesis by DoTPS in D. officinale .
    Keywords terpene synthase ; terpenes ; methyl jasmonate ; abiotic stress ; orchids ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 572
    Language English
    Publishing date 2020-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  4. Article ; Online: Advanced nonlinearity equalizer with TC-NL-MLSE for transmitting beyond 200-Gb/s PAM-8 in IM/DD systems.

    Zhou, Jiahao / Zhang, Jing / Zhao, Xue / Jin, Taowei / Hu, Shaohua / Lin, Hong / Zhenming, Yu / Zhang, Qianwu / Xu, Bo / Qiu, Kun

    Optics express

    2022  Volume 30, Issue 21, Page(s) 37416–37425

    Abstract: The severe band-limited effect resulted from the low-cost optical transceiver increases the channel memory length and the number of taps of the equalizers. Besides, the interaction of fiber dispersion and square-law detection introduce nonlinear ... ...

    Abstract The severe band-limited effect resulted from the low-cost optical transceiver increases the channel memory length and the number of taps of the equalizers. Besides, the interaction of fiber dispersion and square-law detection introduce nonlinear distortions in intensity modulation and direct-detection (IM/DD) transmission systems. The serious band-limited effect and nonlinear distortions degrade the transmission performance and bring challenges to current equalizers for low-complexity implementation. In this paper, we propose a trellis-compression nonlinear maximum likelihood sequence estimation (TC-NL-MLSE) algorithm to compensate the linear and nonlinear distortions with lower complexity. In the TC-NL-MLSE, we introduce a polynomial nonlinear filter (PNLF) to partly compensate both the linear distortions and nonlinear distortions. Then, we establish a look-up-table (LUT) to calculate the nonlinear branch metric (BM). To simplify the calculation, two or three levels with the highest probabilities are selected according to decision thresholds for each symbol to compress the state-trellis graph (STG). This significantly reduces computational complexity on BM calculations especially for high-order modulations. We conduct experiments to transmit beyond the 200-Gb/s PAM-8 signal over 2-km standard single mode fiber (SSMF) at C-band. The TC-NL-MLSE outperforms the reduced-state MLSE with PNLF, and can reach the 7%-overhead hard-decision forward error correction threshold. Moreover, the TC-NL-MLSE reduces the complexity by 97% compared with standard LUT-MLSE, limiting the multipliers around 100 at the expense of only 0.2-dB receiver sensitivity penalty.
    Language English
    Publishing date 2022-10-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1491859-6
    ISSN 1094-4087 ; 1094-4087
    ISSN (online) 1094-4087
    ISSN 1094-4087
    DOI 10.1364/OE.468519
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides

    Can Si / Jaime A. Teixeira da Silva / Chunmei He / Zhenming Yu / Conghui Zhao / Haobin Wang / Mingze Zhang / Jun Duan

    International Journal of Molecular Sciences, Vol 21, Iss 6250, p

    2020  Volume 6250

    Abstract: The acetylation or deacetylation of polysaccharides can influence their physical properties and biological activities. One main constituent of the edible medicinal orchid, Dendrobium officinale , is water-soluble polysaccharides (WSPs) with substituted O ...

    Abstract The acetylation or deacetylation of polysaccharides can influence their physical properties and biological activities. One main constituent of the edible medicinal orchid, Dendrobium officinale , is water-soluble polysaccharides (WSPs) with substituted O -acetyl groups. Both O -acetyl groups and WSPs show a similar trend in different organs, but the genes coding for enzymes that transfer acetyl groups to WSPs have not been identified. In this study, we report that REDUCED WALL ACETYLATION (RWA) proteins may act as acetyltransferases. Three DoRWA genes were identified, cloned, and sequenced. They were sensitive to abscisic acid (ABA), but there were no differences in germination rate and root length between wild type and 35S::DoRWA3 transgenic lines under ABA stress. Three DoRWA proteins were localized in the endoplasmic reticulum. DoRWA3 had relatively stronger transcript levels in organs where acetyl groups accumulated than DoRWA1 and DoRWA2 , was co-expressed with polysaccharides synthetic genes, so it was considered as a candidate acetyltransferase gene. The level of acetylation of polysaccharides increased significantly in the seeds, leaves and stems of three 35S::DoRWA3 transgenic lines compared to wild type plants. These results indicate that DoRWA3 can transfer acetyl groups to polysaccharides and is a candidate protein to improve the biological activity of other edible and medicinal plants.
    Keywords acetyl groups ; Dendrobium officinale ; REDUCED WALL ACETYLATION ; endoplasmic reticulum ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2020-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  6. Article ; Online: Differential Accumulation of Anthocyanins in Dendrobium officinale Stems with Red and Green Peels

    Zhenming Yu / Yinyin Liao / Jaime A. Teixeira da Silva / Ziyin Yang / Jun Duan

    International Journal of Molecular Sciences, Vol 19, Iss 10, p

    2018  Volume 2857

    Abstract: Dendrobium officinale stems, including red and green stems, are widely used as a dietary supplement to develop nutraceutical beverages and food products. However, there is no detailed information on pigment composition of red and green stems. Here, we ... ...

    Abstract Dendrobium officinale stems, including red and green stems, are widely used as a dietary supplement to develop nutraceutical beverages and food products. However, there is no detailed information on pigment composition of red and green stems. Here, we investigated the content and composition of pigments in red and green stems by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry and assessed the differential accumulation of anthocyanins at the molecular level. The color of peels in red stems was caused by the presence of anthocyanins in epidermal cells unlike the peels of green stems. The glucoside derivatives delphinidin and cyanidin are responsible for the red color. Within the D. officinale anthocyanidin biosynthetic pathway, DoANS and DoUFGT, coding for anthocyanidin synthase and UDP-glucose flavonoid-3-O-glucosyltransferase, respectively, are critical regulatory genes related to the differential accumulation of anthocyanidin. These findings provide a more complete profile of pigments, especially anthocyanin, in D. officinale stems, and lay a foundation for producing functional foods.
    Keywords Dendrobium officinale ; anthocyanin ; UPLC-MS-QTOF ; anthocyanidin synthase ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  7. Article ; Online: Differential Accumulation of Aroma Compounds in Normal Green and Albino-Induced Yellow Tea (Camellia sinensis) Leaves

    Fang Dong / Lanting Zeng / Zhenming Yu / Jianlong Li / Jinchi Tang / Xinguo Su / Ziyin Yang

    Molecules, Vol 23, Iss 10, p

    2018  Volume 2677

    Abstract: Tea (Camellia sinensis) cultivars with green leaves are the most widely used for making tea. Recently, tea mutants with white or yellow young shoots have attracted increasing interest as raw materials for making “high-quality” tea products. Albino teas ... ...

    Abstract Tea (Camellia sinensis) cultivars with green leaves are the most widely used for making tea. Recently, tea mutants with white or yellow young shoots have attracted increasing interest as raw materials for making “high-quality” tea products. Albino teas are generallycharacterized as having metabolites of relatively high amino acid content and lower catechin content. However, little is known about aroma compounds in albino tea leaves. Herein, we compared original normal leaves (green) and light-sensitive albino leaves (yellow) of cv. Yinghong No. 9. GC-MS was employed to analyze endogenous tea aroma compounds and related precursors. Quantitative real time PCR was used to measure expression levels of genes involved in biosyntheses of tea aromas.The total contents of most endogenous free tea aromas, including aroma fatty acid derivatives, aroma terpenes, and aroma phenylpropanoids/benzenoids, and their glycosidically bound aroma compounds, were lower in yellow leaves than in green leaves. The content of the key precursor geranyl diphosphate (GDP) and expression levels of key synthetic genes involved in the formation of linalool, a major aroma compound in cv. Yinghong No. 9, were investigated. Linalool content was lower in albino-induced yellow leaves, which was due to the lower GDP content compared with normal green leaves.
    Keywords albino ; aroma ; Camellia sinensis ; geranyl diphosphate ; light-sensitive ; linalool ; tea ; Organic chemistry ; QD241-441
    Subject code 580
    Language English
    Publishing date 2018-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  8. Article ; Online: Genome-Wide Identification and Analysis of the APETALA2 (AP2) Transcription Factor in Dendrobium officinale

    Danqi Zeng / Jaime A. Teixeira da Silva / Mingze Zhang / Zhenming Yu / Can Si / Conghui Zhao / Guangyi Dai / Chunmei He / Juan Duan

    International Journal of Molecular Sciences, Vol 22, Iss 5221, p

    2021  Volume 5221

    Abstract: The APETALA2 (AP2) transcription factors (TFs) play crucial roles in regulating development in plants. However, a comprehensive analysis of the AP2 family members in a valuable Chinese herbal orchid, Dendrobium officinale , or in other orchids, is ... ...

    Abstract The APETALA2 (AP2) transcription factors (TFs) play crucial roles in regulating development in plants. However, a comprehensive analysis of the AP2 family members in a valuable Chinese herbal orchid, Dendrobium officinale , or in other orchids, is limited. In this study, the 14 DoAP2 TFs that were identified from the D. officinale genome and named DoAP2-1 to DoAP2-14 were divided into three clades: euAP2, euANT, and basalANT. The promoters of all DoAP2 genes contained cis -regulatory elements related to plant development and also responsive to plant hormones and stress. qRT-PCR analysis showed the abundant expression of DoAP2-2 , DoAP2-5 , DoAP2-7 , DoAP2-8 and DoAP2-12 genes in protocorm-like bodies (PLBs), while DoAP2-3 , DoAP2-4 , DoAP2-6 , DoAP2-9 , DoAP2-10 and DoAP2-11 expression was strong in plantlets. In addition, the expression of some DoAP2 genes was down-regulated during flower development. These results suggest that DoAP2 genes may play roles in plant regeneration and flower development in D. officinale . Four DoAP2 genes ( DoAP2-1 from euAP2, DoAP2 - 2 from euANT, and DoAP2-6 and DoAP2-11 from basal ANT) were selected for further analyses. The transcriptional activation of DoAP2-1, DoAP2-2, DoAP2-6 and DoAP2-11 proteins, which were localized in the nucleus of Arabidopsis thaliana mesophyll protoplasts, was further analyzed by a dual-luciferase reporter gene system in Nicotiana benthamiana leaves. Our data showed that pBD-DoAP2-1, pBD-DoAP2-2, pBD-DoAP2-6 and pBD-DoAP2-11 significantly repressed the expression of the LUC reporter compared with the negative control (pBD), suggesting that these DoAP2 proteins may act as transcriptional repressors in the nucleus of plant cells. Our findings on AP2 genes in D. officinale shed light on the function of AP2 genes in this orchid and other plant species.
    Keywords Dendrobium officinale ; AP2 transcription factor ; development ; dual-luciferase reporter gene system ; gene expression ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  9. Article ; Online: Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

    Conghui Zhao / Zhenming Yu / Jaime A. Teixeira da Silva / Chunmei He / Haobin Wang / Can Si / Mingze Zhang / Danqi Zeng / Jun Duan

    International Journal of Molecular Sciences, Vol 21, Iss 7005, p

    2020  Volume 7005

    Abstract: Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) ...

    Abstract Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicinal orchid Dendrobium officinale has not yet been identified. In this study, three potential geraniol synthases were mined from the D. officinale genome. DoGES1, which was localized in chloroplasts, was characterized as a geraniol synthase. DoGES1 was highly expressed in flowers, especially in petals. DoGES1 transcript levels were high in the budding stage of D. officinale flowers at 11:00 a.m. DoGES1 catalyzed geraniol in vitro, and transient expression of DoGES1 in Nicotiana benthamiana leaves resulted in the accumulation of geraniol in vivo. These findings on DoGES1 advance our understanding of geraniol biosynthesis in orchids, and lay the basis for genetic modification of floral scent in D. officinale or in other ornamental orchids.
    Keywords floral volatiles ; geraniol ; MEP pathway ; orchids ; terpene synthase ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  10. Article: Molecular cloning and functional analysis of the phosphomannomutase (PMM) gene from Dendrobium officinale and evidence for the involvement of an abiotic stress response during germination

    He, Chunmei / Songjun Zeng / Jaime A. Teixeira da Silva / Zhenming Yu / Jianwen Tan / Jun Duan

    Protoplasma. 2017 July, v. 254, no. 4

    2017  

    Abstract: Phosphomannomutase (PMM, EC 5.4.2.8) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate, the precursor for the synthesis of GDP-mannose. In this study, the complementary DNA (cDNA) of the Phosphomannomutase (PMM) gene was ... ...

    Abstract Phosphomannomutase (PMM, EC 5.4.2.8) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate, the precursor for the synthesis of GDP-mannose. In this study, the complementary DNA (cDNA) of the Phosphomannomutase (PMM) gene was initially cloned from Dendrobium officinale by RACE method. Transient transform result showed that the DoPMM protein was localized in the cytoplasm. The DoPMM gene was highly expressed in the stems of D. officinale both in vegetative and reproductive developmental stages. The putative promoter was cloned by TAIL-PCR and used for searched cis-elements. Stress-related cis-elements like ABRE, TCA-element, and MBS were found in the promoter regions. The DoPMM gene was up-regulated after treatment with abscisic acid, salicylic acid, cold, polyethylene glycol, and NaCl. The total ascorbic acid (AsA) and polysaccharide content in all of the 35S::DoPMM Arabidopsis thaliana transgenic lines #1, #2, and #5 showed a 40, 39, and 31% increase in AsA and a 77, 22, and 39% increase in polysaccharides, respectively more than wild-type (WT) levels. All three 35S::DoPMM transgenic lines exhibited a higher germination percentage than WT plants when seeded on half-strength MS medium supplemented with 150 mM NaCl or 300 mM mannitol. These results provide genetic evidence for the involvement of PMM genes in the biosynthesis of AsA and polysaccharides and the mediation of PMM genes in abiotic stress tolerance during seed germination in A. thaliana.
    Keywords Arabidopsis thaliana ; Dendrobium officinale ; abiotic stress ; abscisic acid ; ascorbic acid ; biosynthesis ; complementary DNA ; cytoplasm ; developmental stages ; gene expression regulation ; genes ; genetically modified organisms ; mannitol ; molecular cloning ; phosphomannomutase ; polyethylene glycol ; polysaccharides ; promoter regions ; salicylic acid ; seed germination ; sodium chloride ; stems ; stress response ; stress tolerance
    Language English
    Dates of publication 2017-07
    Size p. 1693-1704.
    Publishing place Springer Vienna
    Document type Article
    ZDB-ID 123809-7
    ISSN 1615-6102 ; 0033-183X
    ISSN (online) 1615-6102
    ISSN 0033-183X
    DOI 10.1007/s00709-016-1044-1
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

To top