Artikel ; Online: Dynamic RGD ligands derived from highly mobile cyclodextrins regulate spreading and proliferation of endothelial cells to promote vasculogenesis.
International journal of biological macromolecules
2024 Band 267, Heft Pt 2, Seite(n) 131667
Abstract: A thiolated RGD was incorporated into the threaded allyl-β-cyclodextrins (Allyl-β-CDs) of the polyrotaxane (PR) through a thiol-ene click reaction, resulting in the formation of dynamic RGD ligands on the PR surface (dRGD-PR). When maintaining consistent ...
| Abstract | A thiolated RGD was incorporated into the threaded allyl-β-cyclodextrins (Allyl-β-CDs) of the polyrotaxane (PR) through a thiol-ene click reaction, resulting in the formation of dynamic RGD ligands on the PR surface (dRGD-PR). When maintaining consistent RGD density and other physical properties, endothelial cells (ECs) cultured on dRGD-PR exhibited significantly increased cell proliferation and a larger cell spreading area compared to those on the non-dynamic RGD (nRGD-PCL). Furthermore, ECs on dRGD-PR demonstrated elevated expression levels of FAK, p-FAK, and p-AKT, along with a larger population of cells in the G2/M stage during cell cycle analysis, in contrast to cells on nRGD-PCL. These findings suggest that the movement of the RGD ligands may exert additional beneficial effects in promoting EC spreading and proliferation, beyond their essential adhesion and proliferation-promoting capabilities, possibly mediated by the RGD-integrin-FAK-AKT pathway. Moreover, in vitro vasculogenesis tests were conducted using two methods, revealing that ECs cultured on dRGD-PR exhibited much better vasculogenesis than nRGD-PCL in vitro. In vivo testing further demonstrated an increased presence of CD31-positive tissues on dRGD-PR. In conclusion, the enhanced EC spreading and proliferation resulting from the dynamic RGD ligands may contribute to improved in vitro vasculogenesis and in vivo vascularization. |
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| Mesh-Begriff(e) | Humans ; Cell Adhesion/drug effects ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cyclodextrins/chemistry ; Cyclodextrins/pharmacology ; Endothelial Cells/metabolism ; Endothelial Cells/drug effects ; Endothelial Cells/cytology ; Human Umbilical Vein Endothelial Cells/metabolism ; Human Umbilical Vein Endothelial Cells/drug effects ; Ligands ; Neovascularization, Physiologic/drug effects ; Oligopeptides/pharmacology ; Oligopeptides/chemistry ; Poloxamer/chemistry ; Poloxamer/pharmacology ; Proto-Oncogene Proteins c-akt/metabolism ; Rotaxanes |
| Chemische Substanzen | arginyl-glycyl-aspartic acid (78VO7F77PN) ; Cyclodextrins ; Ligands ; Oligopeptides ; Poloxamer (106392-12-5) ; polyrotaxane ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Rotaxanes |
| Sprache | Englisch |
| Erscheinungsdatum | 2024-04-16 |
| Erscheinungsland | Netherlands |
| Dokumenttyp | Journal Article |
| ZDB-ID | 282732-3 |
| ISSN | 1879-0003 ; 0141-8130 |
| ISSN (online) | 1879-0003 |
| ISSN | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2024.131667 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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