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Article ; Online: WTAP promotes microglial M1 polarization by regulating SIRT1

ZHOU Hongxiu / GAO Xu / HOU Shengping

Di-san junyi daxue xuebao, Vol 44, Iss 7, Pp 620-

2022 Volume 629

Abstract: Objective To investigate the role and the possible molecular mechanism of RNA methylase, Wilms' tumor 1-associating protein (WTAP), in microglial activation. Methods HMC3 cells was induced to M1 polarization by treatment of 100 ng/mL IFNγ combined with 1 ...

Abstract	Objective To investigate the role and the possible molecular mechanism of RNA methylase, Wilms' tumor 1-associating protein (WTAP), in microglial activation. Methods HMC3 cells was induced to M1 polarization by treatment of 100 ng/mL IFNγ combined with 1 μg/mL LPS and then served as experimental group, and the untreated cells served as control group. The expression of pro-inflammatory cytokines IL-6, IL-1β, TNF-α and N6-methyladenosine (m6A) at mRNA and protein levels was detected by real-time quantitative PCR and Western blotting, respectively. After HMC3 cells were transfected with RNAi lentivirus to knock down WTAP, the knockdown efficiency was detected by real-time quantitative PCR and Western blotting. Western blot assay was employed to measure the expression levels of pro-inflammatory cytokines IL-6 IL-1β and TNF-α in WTAP knockdown HMC3 cells. Western blotting was also adopted to detect the protein levels of silent mating-type information regulation 2 homolog 1 (SIRT1), COP1, USP18, EP4, pro-inflammatory transcription factors and some inflammatory pathway receptors in the knockdown cells. SIRT1 agonist SRT1720 and SIRT1 inhibitor EX527 were employed to treat HMC3 cells in the blank-virus group and the knockdown WTAP group, respectively, and the expression level of C/EBPβ was detected by Western blotting. Results Treatment of 100 ng/mL IFNγ combined with 1 μg/mL LPS induced significant up-regulation of IL-6, IL-1β and TNF-α expression in HMC3 cells as compared with the untreated cells (P < 0.05). WTAP was highly expressed in M1 polarized HMC3 microglia (P < 0.05). WTAP knockdown resulted in obviously decreased expression levels of pro-inflammatory cytokines IL-6, IL-1β and TNF-α (P < 0.05), increased level of SIRT1 (P < 0.05), and reduced pro-inflammatory transcription factor C/EBPβ (P < 0.05). SIRT1 agonist SRT1720 reduced the relatively high expression of C/EBPβ in the blank-virus group (P < 0.01). While, the inhibitor EX527 increased the relative low expression of C/EBPβ in HMC3 cells with low WTAP knockdown (P < 0.05). Conclusion WTAP promotes M1 polarization in HMC3 cells, possibly through regulating the expression of SIRT1 and thus affecting the C/EBPβ signaling pathway.
Keywords	microglia ; wilms' tumor 1-associating protein ; silent mating-type information regulation 2 homolog 1 ; n6-methyladenosine ; polarization ; Medicine (General) ; R5-920
Subject code	616
Language	Chinese
Publishing date	2022-04-01T00:00:00Z
Publisher	Editorial Office of Journal of Third Military Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Development of a Novel Water Jet Polisher Using Soft Abrasives for Small Complex-Structure Heat Pipes of Aluminum Alloy Produced Using Additive Manufacturing.

Zhang, Tianyu / Zhang, Zhenyu / Feng, Junyuan / Shi, Chunjing / Zhou, Hongxiu / Meng, Fanning / Tong, Dingyi

Materials (Basel, Switzerland)

2024 Volume 17, Issue 3

Abstract: It is a challenge to polish the interior surface of a small bent pipe with complex structures and sizes less than 0.5 mm. This is because of the fact that traditional polishing methods could destroy, block, or break the small complex structures. For a ... ...

Abstract	It is a challenge to polish the interior surface of a small bent pipe with complex structures and sizes less than 0.5 mm. This is because of the fact that traditional polishing methods could destroy, block, or break the small complex structures. For a small bent pipe made of aluminum alloy produced using additive manufacturing, the defects, such as adhered powders and spatters, are easy to jam the pipe without polishing, possibly resulting in catastrophic failure for aerospace applications. To overcome this challenge, a novel water jet polisher was developed using soft polymethyl methacrylate (PMMA) abrasives. After polishing a specific area, the adhered powders on the interior surface were reduced from over 140 to 2, 3, and 6 by the soft abrasives with mesh sizes of 200, 400, and 600, respectively. The surface roughness Sa was decreased from 3.41 to 0.92 μm after polishing using PMMA abrasives with a mesh size of 200. In comparison, silica abrasives were also employed to polish the small bent pipes, leading to the bent part of pipes breaking. However, this kind of failure was absent when using soft abrasives. Computational fluid dynamics calculations elucidate that a peak erosion rate of silica abrasives for a bent pipe with a turn angle of 30° is 2.18 kg/(m
Language	English
Publishing date	2024-01-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma17030582
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Atomic surface achieved through a novel cross-scale model from macroscale to nanoscale.

Zhao, Feng / Zhang, Zhenyu / Deng, Xingqiao / Feng, Junyuan / Zhou, Hongxiu / Liu, Zhensong / Meng, Fanning / Shi, Chunjing

Nanoscale

2024 Volume 16, Issue 5, Page(s) 2318–2336

Abstract: Chemical mechanical polishing (CMP) is widely used to achieve an atomic surface globally, yet its cross-scale polishing mechanisms are elusive. Moreover, traditional CMP normally employs toxic and corrosive slurries, resulting in potential pollution to ... ...

Abstract	Chemical mechanical polishing (CMP) is widely used to achieve an atomic surface globally, yet its cross-scale polishing mechanisms are elusive. Moreover, traditional CMP normally employs toxic and corrosive slurries, resulting in potential pollution to the environment. To overcome these challenges, a novel cross-scale model from the millimeter to nanometer scale is proposed, which was confirmed by a newly developed green CMP process. The developed CMP slurry consisted of hydrogen peroxide, sodium carbonate, sodium hydroxycellulose, and silica. Prior to CMP, fused silica was polished by a ceria slurry. After CMP, the surface roughness (
Language	English
Publishing date	2024-02-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/d3nr05278h
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Article ; Online: [No title information]

Liu, Jiangyi / Liao, Xingyun / Li, Na / Xu, Zongren / Yang, Wang / Zhou, Hongxiu / Liu, Yusen / Zhang, Zhi / Wang, Guoqing / Hou, Shengping

MedComm

2024 Volume 5, Issue 4, Page(s) e534

Abstract: Autoimmune uveitis (AU) is a kind of immune-mediated disease resulting in irreversible ocular damage and even permanent vision loss. However, the precise mechanism underlying dynamic immune changes contributing to disease initiation and progression of AU ...

Title translation	Single‐cell RNA sequencing reveals inflammatory retinal microglia in experimental autoimmune uveitis.
Abstract	Autoimmune uveitis (AU) is a kind of immune-mediated disease resulting in irreversible ocular damage and even permanent vision loss. However, the precise mechanism underlying dynamic immune changes contributing to disease initiation and progression of AU remains unclear. Here, we induced an experimental AU (EAU) model with IRBP
Language	Italian
Publishing date	2024-04-07
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	2688-2663
ISSN (online)	2688-2663
DOI	10.1002/mco2.534
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: N6-methyladenosine demethylase FTO regulates inflammatory cytokine secretion and tight junctions in retinal pigment epithelium cells.

Tang, Shiyun / Meng, Jiayu / Tan, Jun / Liu, Xianyang / Zhou, Hongxiu / Li, Na / Hou, Shengping

Clinical immunology (Orlando, Fla.)

2022 Volume 241, Page(s) 109080

Abstract: Objective: Uveitis is an intraocular inflammatory disease. Epigenetics has been associated with its pathogenesis. However, the role of N6-methyladenosine (m6A) in uveitis has not been reported. We aimed to examine the role of m6A and its regulatory ... ...

Abstract	Objective: Uveitis is an intraocular inflammatory disease. Epigenetics has been associated with its pathogenesis. However, the role of N6-methyladenosine (m6A) in uveitis has not been reported. We aimed to examine the role of m6A and its regulatory mechanism in experimental autoimmune uveitis (EAU). Methods: The mRNA expression of m6A-related methylase and demethylase of retinal pigment epithelium (RPE) between mice with EAU and control mice was detected by RT-qPCR. The overall m6A level of ARPE-19 cells was detected by an m6A quantitative detection kit. Cell proliferation was observed by CCK-8 assays, and ELISA was used to test the secretion of inflammatory factors. The expression of tight junction proteins and the target genes of FTO were examined by western blotting and MeRIP-PCR. Results: A decreased expression of FTO in RPE cells was found in mice with EAU. Increased overall m6A%, proliferation of cells and secretion of IL-6, IL-8 and MCP-1 were found after FTO knockdown in ARPE-19 cells. However, ZO-1 and occludin protein expression was decreased. ATF4 protein expression was decreased in the FTO knockdown (shFTO) group as compared with the control (shNC) group. In contrast, the m6A level of ATF4 was elevated, as shown by MeRIP-PCR. Functional analysis showed that p-STAT3 expression was increased in the shFTO group, and the change in occludin expression was reversed in ATF4 rescue experiment. Conclusion: FTO may affect the translation of ATF4 by regulating its m6A level, resulting in the increased expression of p-STAT3 and inflammatory factors, and leading to uveitis.
MeSH term(s)	Adenosine/analogs & derivatives ; Adenosine/metabolism ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO/genetics ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO/metabolism ; Animals ; Cytokines/metabolism ; Mice ; Occludin/metabolism ; Retinal Pigment Epithelium/cytology ; Retinal Pigment Epithelium/metabolism ; Tight Junctions/metabolism ; Uveitis/genetics
Chemical Substances	Cytokines ; Occludin ; N-methyladenosine (CLE6G00625) ; FTO protein, mouse (EC 1.14.11.-) ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO (EC 1.14.11.33) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2022-07-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1459903-x
ISSN	1521-7035 ; 1521-6616
ISSN (online)	1521-7035
ISSN	1521-6616
DOI	10.1016/j.clim.2022.109080
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Article ; Online: Unprecedented atomic surface of silicon induced by environmentally friendly chemical mechanical polishing.

Cui, Xiangxiang / Zhang, Zhenyu / Yu, Shiqiang / Chen, Xin / Shi, Chunjing / Zhou, Hongxiu / Meng, Fanning / Yu, Jiaxin / Wen, Wei

Nanoscale

2023 Volume 15, Issue 21, Page(s) 9304–9314

Abstract: Silicon (Si) dominates the integrated circuit (IC), semiconductor, and microelectronic industries. However, it is a challenge to achieve a sub-angstrom surface of Si. Chemical mechanical polishing (CMP) is widely used in the manufacturing of Si, while ... ...

Abstract	Silicon (Si) dominates the integrated circuit (IC), semiconductor, and microelectronic industries. However, it is a challenge to achieve a sub-angstrom surface of Si. Chemical mechanical polishing (CMP) is widely used in the manufacturing of Si, while toxic and polluted slurries are usually employed in CMP, resulting in pollution to the environment. In this study, a novel environmentally friendly CMP was developed, in which a slurry is composed of ceria, hydrogen peroxide, sodium pyrophosphate, sodium carboxymethyl cellulose, sodium carbonate, and deionized water. After CMP, the surface roughness Sa was 0.067 nm with a measurement area of 50 × 50 μm
Language	English
Publishing date	2023-06-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/d3nr01149f
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Atomic surface of quartz glass induced by photocatalytic green chemical mechanical polishing using the developed SiO

Fan, Yuanhang / Zhang, Zhenyu / Yu, Jiaxin / Deng, Xingqiao / Shi, Chunjing / Zhou, Hongxiu / Meng, Fanning / Feng, Junyuan

Nanoscale advances

2023 Volume 6, Issue 5, Page(s) 1380–1391

Abstract: High-performance devices of quartz glass demand an atomic surface, which induces a challenge for chemical mechanical polishing (CMP) with a high material removal rate (MRR). Moreover, traditional CMP usually employs toxic and corrosive slurries, leading ... ...

Abstract	High-performance devices of quartz glass demand an atomic surface, which induces a challenge for chemical mechanical polishing (CMP) with a high material removal rate (MRR). Moreover, traditional CMP usually employs toxic and corrosive slurries, leading to the pollution of the environment. To overcome these challenges, a novel green photocatalytic CMP is proposed. In the CMP, SiO
Language	English
Publishing date	2023-12-12
Publishing country	England
Document type	Journal Article
ISSN	2516-0230
ISSN (online)	2516-0230
DOI	10.1039/d3na00991b
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Low Expression of YTH Domain-Containing 1 Promotes Microglial M1 Polarization by Reducing the Stability of Sirtuin 1 mRNA.

Zhou, Hongxiu / Xu, Zongren / Liao, Xingyun / Tang, Shiyun / Li, Na / Hou, Shengping

Frontiers in cellular neuroscience

2021 Volume 15, Page(s) 774305

Abstract: The N6-methyladenosine (m6A) modification is the most abundant posttranscriptional mRNA modification in mammalian cells and is dynamically modulated by a series of "writers," "erasers," and "readers." Studies have shown that m6A affects RNA metabolism in ...

Abstract	The N6-methyladenosine (m6A) modification is the most abundant posttranscriptional mRNA modification in mammalian cells and is dynamically modulated by a series of "writers," "erasers," and "readers." Studies have shown that m6A affects RNA metabolism in terms of RNA processing, nuclear export, translation, and decay. However, the role of the m6A modification in retinal microglial activation remains unclear. Here, we analyzed the single-cell RNA sequencing data of retinal cells from mice with uveitis and found that the m6A-binding protein YTH domain-containing 1 (YTHDC1) was significantly downregulated in retinal microglia in the context of uveitis. Further studies showed that YTHDC1 deficiency resulted in M1 microglial polarization, an increased inflammatory response and the promotion of microglial migration. Mechanistically, YTHDC1 maintained sirtuin 1 (SIRT1) mRNA stability, which reduced signal transducer and activator of transcription 3 (STAT3) phosphorylation, thus inhibiting microglial M1 polarization. Collectively, our data show that YTHDC1 is critical for microglial inflammatory response regulation and can serve as a target for the development of therapeutics for autogenic immune diseases.
Language	English
Publishing date	2021-12-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2452963-1
ISSN	1662-5102
ISSN	1662-5102
DOI	10.3389/fncel.2021.774305
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: A Single-Cell Transcriptome Atlas of the Human Retinal Pigment Epithelium.

Xu, Zongren / Liao, Xingyun / Li, Na / Zhou, Hongxiu / Li, Hong / Zhang, Qi / Hu, Ke / Yang, Peizeng / Hou, Shengping

Frontiers in cell and developmental biology

2021 Volume 9, Page(s) 802457

Abstract: Human retinal pigment epithelium cells are arranged in a monolayer that plays an important supporting role in the retina. Although the heterogeneity of specific retinal cells has been well studied, the diversity of hRPE cells has not been reported. Here, ...

Abstract	Human retinal pigment epithelium cells are arranged in a monolayer that plays an important supporting role in the retina. Although the heterogeneity of specific retinal cells has been well studied, the diversity of hRPE cells has not been reported. Here, we performed a single-cell RNA sequencing on 9,302 hRPE cells from three donors and profiled a transcriptome atlas. Our results identified two subpopulations that exhibit substantial differences in gene expression patterns and functions. One of the clusters specifically expressed
Language	English
Publishing date	2021-12-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2021.802457
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Article: Geoprocessing in Cloud Computing platforms – a comparative analysis

Yue, Peng / Zhou, Hongxiu / Gong, Jianya / Hu, Lei

International journal of digital earth. 2013 July 1, v. 6, no. 4

2013

Abstract: The emergence of Cloud Computing technologies brings a new information infrastructure to users. Providing geoprocessing functions in Cloud Computing platforms can bring scalable, on-demand, and cost–effective geoprocessing services to geospatial users. ... ...

Abstract	The emergence of Cloud Computing technologies brings a new information infrastructure to users. Providing geoprocessing functions in Cloud Computing platforms can bring scalable, on-demand, and cost–effective geoprocessing services to geospatial users. This paper provides a comparative analysis of geoprocessing in Cloud Computing platforms – Microsoft Windows Azure and Google App Engine. The analysis compares differences in the data storage, architecture model, and development environment based on the experience to develop geoprocessing services in the two Cloud Computing platforms; emphasizes the importance of virtualization; recommends applications of hybrid geoprocessing Clouds, and suggests an interoperable solution on geoprocessing Cloud services. The comparison allows one to selectively utilize Cloud Computing platforms or hybrid Cloud pattern, once it is understood that the current development of geoprocessing Cloud services is restricted to specific Cloud Computing platforms with certain kinds of technologies. The performance evaluation is also performed over geoprocessing services deployed in public Cloud platforms. The tested services are developed using geoprocessing algorithms from different vendors, GeoSurf and Java Topology Suite. The evaluation results provide a valuable reference on providing elastic and cost-effective geoprocessing Cloud services.
Keywords	algorithms ; cost effectiveness ; infrastructure ; models
Language	English
Dates of publication	2013-0701
Size	p. 404-425.
Publishing place	Taylor & Francis Group
Document type	Article
ISSN	1753-8955
DOI	10.1080/17538947.2012.748847
Database	NAL-Catalogue (AGRICOLA)

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