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  1. Article: Genetic Diversity and Gene Flow Observed in Two Cereal Aphid (Hemiptera: Aphididae) Species and Populations in the Chinese Corn Belt Region

    Sun, Wei / Su, Qianfu / Yang, Wei / Zhou, Jiachun / Gao, Yuebo

    Journal of entomological science. 2022 June 22, v. 57, no. 3

    2022  

    Abstract: The cereal aphids, Rhopalosiphum maidis (Fitch) and Rhopalosiphum padi (L.) (Hemiptera: Aphididae), are serious pests of maize, Zea mays L., globally. Basic information about the genetic structure of these aphids is unknown. We, therefore, estimated the ... ...

    Abstract The cereal aphids, Rhopalosiphum maidis (Fitch) and Rhopalosiphum padi (L.) (Hemiptera: Aphididae), are serious pests of maize, Zea mays L., globally. Basic information about the genetic structure of these aphids is unknown. We, therefore, estimated the genetic diversity and genetic flow from partial fragments of the mitochondrial cytochrome oxidase I gene collected from different geographic populations of aphids throughout the Songliao Plain of northeastern China. Our analysis of a 425-bp sequence on 297 R. maidis and 287 R. padi individuals revealed 17 and 3 variable sites, respectively. Fifteen haplotypes were obtained among the R. maidis populations, and only haplotype RM1 was shared. Three shared haplotypes were obtained among the R. padi populations. No obvious geographical trends were detected based on the haplotype network and neighbor-joining tree. Relatively low haplotype diversity indices were observed in the R. maidis populations (haplotype diversity [Hd] = 0.14207, average number of nucleotide differences [K] = 0.17340, and nucleotide diversity [Pi] = 0.00041), whereas relatively high haplotype diversity indices were observed in the R. padi populations (Hd = 0.53249, K = 1.46614, and Pi = 0.00345). There was moderate gene flow (number of migrants [Nm] = 2.33) among R. maidis populations, but there was low gene flow (Nm = 0.82) among R. padi populations. Analysis of molecular variance showed high genetic differentiation within populations. Genetic distance and geographic distance were not significantly associated according to the Mantel test. The results suggest that the difference in the aphids' existence strategies has resulted in a different mitochondrial evolution pattern in the Songliao Plain region, and they provide a foundation for accurately forecasting systems against this pest.
    Keywords Rhopalosiphum maidis ; Rhopalosiphum padi ; Zea mays ; corn ; cytochrome-c oxidase ; gene flow ; genes ; genetic distance ; genetic variation ; haplotypes ; mitochondria ; variance ; China ; Corn Belt region
    Language English
    Dates of publication 2022-0622
    Size p. 363-379.
    Publishing place Georgia Entomological Society
    Document type Article
    ZDB-ID 12629-9
    ISSN 0749-8004
    ISSN 0749-8004
    DOI 10.18474/JES21-64
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    Z 4546: Show issues

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  2. Article: Destinations of third-generation Mythimna separata (Lepidoptera: Noctuidae) moths in Jilin and its effects on population genetic diversity

    Sun, Wei / Su, Qianfu / Yang, Wei / Zhou, Jiachun / Gao, Yuebo

    Applied entomology and zoology. 2022 Nov., v. 57, no. 4

    2022  

    Abstract: Third-generation oriental armyworm larvae, Mythimna separata (Walker) (Lepidoptera: Noctuidae), have caused considerable economic losses in Jilin. Understanding the occurrence, regularity and return movement of third-generation adults is crucial for pest ...

    Abstract Third-generation oriental armyworm larvae, Mythimna separata (Walker) (Lepidoptera: Noctuidae), have caused considerable economic losses in Jilin. Understanding the occurrence, regularity and return movement of third-generation adults is crucial for pest forecasting. The study was conducted using systemic monitoring, radar observations and molecular markers from 2015 to 2019. The main results are as follows: (1) third-generation moths were regularly captured from late August to late September. These moths have been attributed to local emergence and were identified as an “emigration population.” Wind speed and temperature were advantageous for moth migration, and advantageous northeasterly winds account for 9.6% of the total. Radar studies determined that the migrant flying height was mainly below 600 m. Moths migrated over a range of directions, including their return movements. Forward trajectories predicted that the destination regions of the Korean Peninsula, the Russian Far East, and northern China were unfavorable for survival. (2) A total of 29 haplotypes were detected. There were unique haplotypes between two sampling years. The haplotype analysis indicated similar content in the two sampling years. The haplotype diversity indices appear to have been maintained. Overall, our findings indicate that the moths did not make a successful return flight, but the genetic diversity was unaffected.
    Keywords Mythimna separata ; Russia ; entomology ; flight ; genetic variation ; haplotypes ; moths ; pests ; population genetics ; radar ; temperature ; wind speed ; China ; Korean Peninsula
    Language English
    Dates of publication 2022-11
    Size p. 333-345.
    Publishing place Springer Japan
    Document type Article
    ZDB-ID 820195-x
    ISSN 1347-605X ; 0003-6862
    ISSN (online) 1347-605X
    ISSN 0003-6862
    DOI 10.1007/s13355-022-00792-8
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  3. Article: Population Source of Third-Generation Oriental Armyworm in Jilin, China, Determined by Entomology Radar, Trajectory Analysis, and Mitochondrial COI Sequences

    Sun, Wei / Hu, Gao / Su, Qianfu / Wang, Yangzhou / Yang, Wei / Zhou, Jiachun / Gao, Yuebo

    Environmental entomology. 2022 Apr. 7, v. 51, no. 3

    2022  

    Abstract: The armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae), is an important polyphagous pest with a strong migratory ability. Recently, third-generation larvae have become an increasingly serious pest threat in Jilin Province of northeast China. ... ...

    Abstract The armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae), is an important polyphagous pest with a strong migratory ability. Recently, third-generation larvae have become an increasingly serious pest threat in Jilin Province of northeast China. To investigate the population source of this species, scanning entomological radar observations and insect mitochondrial cytochrome oxidase I (COI) genes were used in this study. Five main results were found: (1) The peak period in captured second-generation moths was from mid to late July. The temperature and wind speeds were optimum for the moths to have migrated. Strong southwesterly winds occurred during the peak migration period. (2) Radar observations indicated that most of the moths' migration took place at a height of 600 m, often in a dense layer which formed at heights of 350–800 m. (3) Analyses of adult ovarian development and larval haplotypes showed third-generation larvae were progeny of both locally produced progeny and immigrant moths. (4) Based on our back-tracking and haplotype analyses, immigration led to an outbreak originated in the same source area to the southwest. (5) Emigration of second-generation moths was confirmed by both radar observation and mtDNA analysis. Forward trajectories indicated that the moths were capable of immigrating far from their overwintering range. These results are useful for improving the forecasting systems of this insect pest species.
    Keywords Mythimna separata ; adults ; cytochrome-c oxidase ; entomology ; haplotypes ; immigration ; insect pests ; larvae ; migratory behavior ; mitochondria ; ovarian development ; overwintering ; progeny ; radar ; temperature ; China
    Language English
    Dates of publication 2022-0407
    Size p. 621-632.
    Publishing place Entomological Society of America
    Document type Article
    ISSN 0046-225X
    DOI 10.1093/ee/nvac020
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  4. Article ; Online: Population Source of Third-Generation Oriental Armyworm in Jilin, China, Determined by Entomology Radar, Trajectory Analysis, and Mitochondrial COI Sequences.

    Sun, Wei / Hu, Gao / Su, Qianfu / Wang, Yangzhou / Yang, Wei / Zhou, Jiachun / Gao, Yuebo

    Environmental entomology

    2022  Volume 51, Issue 3, Page(s) 621–632

    Abstract: The armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae), is an important polyphagous pest with a strong migratory ability. Recently, third-generation larvae have become an increasingly serious pest threat in Jilin Province of northeast China. ... ...

    Abstract The armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae), is an important polyphagous pest with a strong migratory ability. Recently, third-generation larvae have become an increasingly serious pest threat in Jilin Province of northeast China. To investigate the population source of this species, scanning entomological radar observations and insect mitochondrial cytochrome oxidase I (COI) genes were used in this study. Five main results were found: (1) The peak period in captured second-generation moths was from mid to late July. The temperature and wind speeds were optimum for the moths to have migrated. Strong southwesterly winds occurred during the peak migration period. (2) Radar observations indicated that most of the moths' migration took place at a height of 600 m, often in a dense layer which formed at heights of 350-800 m. (3) Analyses of adult ovarian development and larval haplotypes showed third-generation larvae were progeny of both locally produced progeny and immigrant moths. (4) Based on our back-tracking and haplotype analyses, immigration led to an outbreak originated in the same source area to the southwest. (5) Emigration of second-generation moths was confirmed by both radar observation and mtDNA analysis. Forward trajectories indicated that the moths were capable of immigrating far from their overwintering range. These results are useful for improving the forecasting systems of this insect pest species.
    MeSH term(s) Animals ; China ; Larva/genetics ; Moths/genetics ; Radar ; Spodoptera
    Language English
    Publishing date 2022-04-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120799-4
    ISSN 1938-2936 ; 0046-2268 ; 0046-225X
    ISSN (online) 1938-2936
    ISSN 0046-2268 ; 0046-225X
    DOI 10.1093/ee/nvac020
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    In stock of ZB MED Bonn / Germany

    Z 2779: Show issues

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  5. Article: One-step immobilization-purification of enzymes by carbohydrate-binding module family 56 tag fusion

    Qin, Zhen / Lin, Si / Qiu, Yongjun / Chen, Qiming / Zhang, Yin / Zhou, Jiachun / Zhao, Liming

    Food chemistry. 2019 Nov. 30, v. 299

    2019  

    Abstract: Immobilization of enzymes is an essential strategy with outstanding prospects in biocatalytic processes. Nontoxic, inexpensive immobilized enzyme approach is especially important for food enzymes. We here demonstrate that a carbohydrate-binding module ... ...

    Abstract Immobilization of enzymes is an essential strategy with outstanding prospects in biocatalytic processes. Nontoxic, inexpensive immobilized enzyme approach is especially important for food enzymes. We here demonstrate that a carbohydrate-binding module family 56 domain (CBM56-Tag) mediates the immobilization of fusion enzymes with the curdlan (β-1,3-glucan) particle support, thereby enabling the one-step immobilization-purification of target enzymes. CBM56-Tag exhibits an immunoglobulin-like β-sandwich fold, which can be adsorbed by curdlan via hydrogen bond-mediated binding. The maximum adsorption capacity of a fusion chitosanase (CBM56-GsCsn46A) on curdlan is 50.72 mg/g. The immobilized enzyme could be directly used in the packed-bed reactor. This immobilization strategy utilizes a natural polysaccharide without any treatment, avoiding the negative environmental effects. Moreover, the one step immobilization-purification simplifies the purification step, which reduces the use of chemicals. Our study provides a nontoxic and inexpensive immobilization strategy for the biocatalytic reaction in food industry.
    Keywords adsorption ; beta-glucans ; biocatalysis ; carbohydrate binding ; chitosanase ; curdlan ; environmental impact ; food industry ; hydrogen ; immobilized enzymes
    Language English
    Dates of publication 2019-1130
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2019.125037
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  6. Article: Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice

    Li, Wei / Zhao, Mengyao / Qin, Zhen / Chen, Qiming / Fan, Liqiang / Zhou, Jiachun / Zhao, Liming

    Journal of food biochemistry. 2019 May, v. 43, no. 5

    2019  

    Abstract: This study investigated the intervention effects of chitooligosaccharides (COS) on retinol metabolism and included comparisons of the retinol level, retinol binding protein 4 (RBP4) content, key genes, and protein expression between mice on a COS‐ ... ...

    Abstract This study investigated the intervention effects of chitooligosaccharides (COS) on retinol metabolism and included comparisons of the retinol level, retinol binding protein 4 (RBP4) content, key genes, and protein expression between mice on a COS‐enriched diet and a normal diet. The results showed that COS markedly decreased the retinol and RBP4 concentrations in the serum and liver. Furthermore, COS suppressed the mRNA and protein expression of RBP4, cellular retinol binding protein 1 (CRBP1), lecithin: retinol acyltransferase (LRAT) and cytochrome P45026A1 (CYP26A1). In addition, COS inhibited the mRNA expression of stimulated by retinoic acid 6 (STRA6). However, the protein expression of STRA6 was not significantly decreased. Thus, COS reduced the retinol concentration in the serum and disrupted the metabolism of retinol. The intervention mechanism of COS on retinol metabolism may be attributed to the modulation of RBP4, CRBP1, LRAT, STRA6, and CYP26A1 expression at the mRNA and protein levels. PRACTICAL APPLICATIONS: Chitooligosaccharides (COS), known to be the degradation products of chitosan, have been found to induce pinkeye in industrial workers who participate in the manufacturing of COS. Meanwhile, 5% population with COS dietary supplement also have similar phenomenon. The aim of this study is to explore the possible mechanism underlay of this potential risk. The results of this study showed that high exposure to COS during manufacture influences retinol metabolism and leads to a decrease in retinol content, ultimately causing pinkeye. These findings provide new evidence for understanding COS‐induced retinol metabolism alteration and drawing attention toward the prevention of potential risk in high‐exposure populations.
    Keywords bioavailability ; blood serum ; chitooligosaccharides ; chitosan ; dietary supplements ; gene expression ; genes ; keratoconjunctivitis ; lecithins ; liver ; messenger RNA ; mice ; nutrition risk assessment ; protein content ; protein synthesis ; retinoic acid ; retinol-binding protein ; transferases ; vitamin A
    Language English
    Dates of publication 2019-05
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 433846-7
    ISSN 1745-4514 ; 0145-8884
    ISSN (online) 1745-4514
    ISSN 0145-8884
    DOI 10.1111/jfbc.12831
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  7. Article: Efficient Immobilization of Bacterial GH Family 46 Chitosanase by Carbohydrate-Binding Module Fusion for the Controllable Preparation of Chitooligosaccharides

    Lin, Si / Qin, Zhen / Chen, Qiming / Fan, Liqiang / Zhou, Jiachun / Zhao, Liming

    Journal of agricultural and food chemistry. 2019 May 27, v. 67, no. 24

    2019  

    Abstract: Chitooligosaccharide has been reported to possess diverse bioactivities. The development of novel strategies for obtaining optimum degree of polymerization (DP) chitooligosaccharides has become increasingly important. In this study, two glycoside ... ...

    Abstract Chitooligosaccharide has been reported to possess diverse bioactivities. The development of novel strategies for obtaining optimum degree of polymerization (DP) chitooligosaccharides has become increasingly important. In this study, two glycoside hydrolase family 46 chitosanases were studied for immobilization on curdlan (insoluble β-1,3-glucan) using a novel carbohydrate binding module (CBM) family 56 domain from a β-1,3-glucanase. The CBM56 domain provided a spontaneous and specific sorption of the fusion proteins onto a curdlan carrier, and two fusion enzymes showed increased enzyme stability in comparison with native enzymes. Furthermore, a continuous packed-bed reactor was constructed with chitosanase immobilized on a curdlan carrier to control the enzymatic hydrolysis of chitosan. Three chitooligosaccharide products with different molecular weights were prepared in optimized reaction conditions. This study provides a novel CBM tag for the stabilization and immobilization of enzymes. The controllable hydrolysis strategy offers potential for the industrial-scale preparation of chitooligosaccharides with different desired DPs.
    Keywords beta-glucanase ; beta-glucans ; bioactive properties ; carbohydrate binding ; chitooligosaccharides ; chitosan ; chitosanase ; curdlan ; enzymatic hydrolysis ; enzyme stability ; glycosides ; hydrolysis ; molecular weight ; polymerization ; sorption
    Language English
    Dates of publication 2019-0527
    Size p. 6847-6855.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.9b01608
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    Z 1185: Show issues

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  8. Article ; Online: Chitopentaose inhibits hepatocellular carcinoma by inducing mitochondrial mediated apoptosis and suppressing protective autophagy.

    Zhu, Chunfeng / Zhao, Mengyao / Fan, Liqiang / Cao, Xuni / Xia, Quanming / Zhou, Jiachun / Yin, Hao / Zhao, Liming

    Bioresources and bioprocessing

    2021  Volume 8, Issue 1, Page(s) 4

    Abstract: Hepatocellular carcinoma (HCC) is one of the most prevalent and deadliest cancers. In this study, the anti-tumor effect of singular degree of polymerization (DP) chitooligosaccharides (COS) (DP 2-5) and the underlay molecular mechanisms were investigated ...

    Abstract Hepatocellular carcinoma (HCC) is one of the most prevalent and deadliest cancers. In this study, the anti-tumor effect of singular degree of polymerization (DP) chitooligosaccharides (COS) (DP 2-5) and the underlay molecular mechanisms were investigated on HCC cell line HepG2. MTT assay showed that (GlcN)
    Language English
    Publishing date 2021-01-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2785482-6
    ISSN 2197-4365 ; 2197-4365
    ISSN (online) 2197-4365
    ISSN 2197-4365
    DOI 10.1186/s40643-020-00358-y
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  9. Article ; Online: One-step immobilization-purification of enzymes by carbohydrate-binding module family 56 tag fusion.

    Qin, Zhen / Lin, Si / Qiu, Yongjun / Chen, Qiming / Zhang, Yin / Zhou, Jiachun / Zhao, Liming

    Food chemistry

    2019  Volume 299, Page(s) 125037

    Abstract: Immobilization of enzymes is an essential strategy with outstanding prospects in biocatalytic processes. Nontoxic, inexpensive immobilized enzyme approach is especially important for food enzymes. We here demonstrate that a carbohydrate-binding module ... ...

    Abstract Immobilization of enzymes is an essential strategy with outstanding prospects in biocatalytic processes. Nontoxic, inexpensive immobilized enzyme approach is especially important for food enzymes. We here demonstrate that a carbohydrate-binding module family 56 domain (CBM56-Tag) mediates the immobilization of fusion enzymes with the curdlan (β-1,3-glucan) particle support, thereby enabling the one-step immobilization-purification of target enzymes. CBM56-Tag exhibits an immunoglobulin-like β-sandwich fold, which can be adsorbed by curdlan via hydrogen bond-mediated binding. The maximum adsorption capacity of a fusion chitosanase (CBM56-GsCsn46A) on curdlan is 50.72 mg/g. The immobilized enzyme could be directly used in the packed-bed reactor. This immobilization strategy utilizes a natural polysaccharide without any treatment, avoiding the negative environmental effects. Moreover, the one step immobilization-purification simplifies the purification step, which reduces the use of chemicals. Our study provides a nontoxic and inexpensive immobilization strategy for the biocatalytic reaction in food industry.
    MeSH term(s) Biocatalysis ; Enzymes, Immobilized/chemistry ; Enzymes, Immobilized/isolation & purification ; Enzymes, Immobilized/metabolism ; Glycoside Hydrolases/chemistry ; Glycoside Hydrolases/isolation & purification ; Glycoside Hydrolases/metabolism ; Hydrogen Bonding ; Receptors, Cell Surface/metabolism ; Recombinant Fusion Proteins/metabolism ; beta-Glucans/chemistry
    Chemical Substances Enzymes, Immobilized ; Receptors, Cell Surface ; Recombinant Fusion Proteins ; beta-Glucans ; saccharide-binding proteins ; curdlan (6930DL209R) ; Glycoside Hydrolases (EC 3.2.1.-) ; chitosanase (EC 3.2.1.132)
    Language English
    Publishing date 2019-06-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2019.125037
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    In stock of ZB MED Bonn / Germany

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  10. Article ; Online: Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.

    Li, Wei / Zhao, Mengyao / Qin, Zhen / Chen, Qiming / Fan, Liqiang / Zhou, Jiachun / Zhao, Liming

    Journal of food biochemistry

    2019  Volume 43, Issue 5, Page(s) e12831

    Abstract: This study investigated the intervention effects of chitooligosaccharides (COS) on retinol metabolism and included comparisons of the retinol level, retinol binding protein 4 (RBP4) content, key genes, and protein expression between mice on a COS- ... ...

    Abstract This study investigated the intervention effects of chitooligosaccharides (COS) on retinol metabolism and included comparisons of the retinol level, retinol binding protein 4 (RBP4) content, key genes, and protein expression between mice on a COS-enriched diet and a normal diet. The results showed that COS markedly decreased the retinol and RBP4 concentrations in the serum and liver. Furthermore, COS suppressed the mRNA and protein expression of RBP4, cellular retinol binding protein 1 (CRBP1), lecithin: retinol acyltransferase (LRAT) and cytochrome P45026A1 (CYP26A1). In addition, COS inhibited the mRNA expression of stimulated by retinoic acid 6 (STRA6). However, the protein expression of STRA6 was not significantly decreased. Thus, COS reduced the retinol concentration in the serum and disrupted the metabolism of retinol. The intervention mechanism of COS on retinol metabolism may be attributed to the modulation of RBP4, CRBP1, LRAT, STRA6, and CYP26A1 expression at the mRNA and protein levels. PRACTICAL APPLICATIONS: Chitooligosaccharides (COS), known to be the degradation products of chitosan, have been found to induce pinkeye in industrial workers who participate in the manufacturing of COS. Meanwhile, 5% population with COS dietary supplement also have similar phenomenon. The aim of this study is to explore the possible mechanism underlay of this potential risk. The results of this study showed that high exposure to COS during manufacture influences retinol metabolism and leads to a decrease in retinol content, ultimately causing pinkeye. These findings provide new evidence for understanding COS-induced retinol metabolism alteration and drawing attention toward the prevention of potential risk in high-exposure populations.
    MeSH term(s) Acyltransferases/genetics ; Acyltransferases/metabolism ; Animals ; Chitin/adverse effects ; Chitin/analogs & derivatives ; Chitin/pharmacology ; Chitosan/metabolism ; Eye Diseases/prevention & control ; Humans ; Mice ; Occupational Exposure/prevention & control ; Retinoic Acid 4-Hydroxylase/genetics ; Retinoic Acid 4-Hydroxylase/metabolism ; Retinol-Binding Proteins, Cellular/genetics ; Retinol-Binding Proteins, Cellular/metabolism ; Retinol-Binding Proteins, Plasma/genetics ; Retinol-Binding Proteins, Plasma/metabolism ; Risk ; Vitamin A/blood ; Vitamin A/metabolism
    Chemical Substances CrbpI protein, mouse ; Rbp4 protein, mouse ; Retinol-Binding Proteins, Cellular ; Retinol-Binding Proteins, Plasma ; oligochitosan ; Vitamin A (11103-57-4) ; Chitin (1398-61-4) ; Chitosan (9012-76-4) ; Cyp26a1 protein, mouse (EC 1.14.14.1) ; Retinoic Acid 4-Hydroxylase (EC 1.14.14.1) ; Acyltransferases (EC 2.3.-) ; lecithin-retinol acyltransferase (EC 2.3.1.-)
    Language English
    Publishing date 2019-03-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 433846-7
    ISSN 1745-4514 ; 0145-8884
    ISSN (online) 1745-4514
    ISSN 0145-8884
    DOI 10.1111/jfbc.12831
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