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  1. Article ; Online: Self-supervised learning for medical image data with anatomy-oriented imaging planes.

    Zhang, Tianwei / Wei, Dong / Zhu, Mengmeng / Gu, Shi / Zheng, Yefeng

    Medical image analysis

    2024  Volume 94, Page(s) 103151

    Abstract: Self-supervised learning has emerged as a powerful tool for pretraining deep networks on unlabeled data, prior to transfer learning of target tasks with limited annotation. The relevance between the pretraining pretext and target tasks is crucial to the ... ...

    Abstract Self-supervised learning has emerged as a powerful tool for pretraining deep networks on unlabeled data, prior to transfer learning of target tasks with limited annotation. The relevance between the pretraining pretext and target tasks is crucial to the success of transfer learning. Various pretext tasks have been proposed to utilize properties of medical image data (e.g., three dimensionality), which are more relevant to medical image analysis than generic ones for natural images. However, previous work rarely paid attention to data with anatomy-oriented imaging planes, e.g., standard cardiac magnetic resonance imaging views. As these imaging planes are defined according to the anatomy of the imaged organ, pretext tasks effectively exploiting this information can pretrain the networks to gain knowledge on the organ of interest. In this work, we propose two complementary pretext tasks for this group of medical image data based on the spatial relationship of the imaging planes. The first is to learn the relative orientation between the imaging planes and implemented as regressing their intersecting lines. The second exploits parallel imaging planes to regress their relative slice locations within a stack. Both pretext tasks are conceptually straightforward and easy to implement, and can be combined in multitask learning for better representation learning. Thorough experiments on two anatomical structures (heart and knee) and representative target tasks (semantic segmentation and classification) demonstrate that the proposed pretext tasks are effective in pretraining deep networks for remarkably boosted performance on the target tasks, and superior to other recent approaches.
    MeSH term(s) Humans ; Heart/diagnostic imaging ; Knee Joint ; Semantics ; Supervised Machine Learning ; Image Processing, Computer-Assisted
    Language English
    Publishing date 2024-03-21
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1356436-5
    ISSN 1361-8423 ; 1361-8431 ; 1361-8415
    ISSN (online) 1361-8423 ; 1361-8431
    ISSN 1361-8415
    DOI 10.1016/j.media.2024.103151
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: AX·(H

    Zhu, Mengmeng / Wang, Junbo / Hou, Lei / Yuan, Yiwen / Liu, Lili / Chu, Yaoqing / Huang, Chunmei

    Inorganic chemistry

    2024  Volume 63, Issue 4, Page(s) 2289–2297

    Abstract: The design and syntheses of new birefringent crystals will be of great importance in commercial applications and materials science. A series of ultraviolet (UV) birefringent crystals, AX·( ... ...

    Abstract The design and syntheses of new birefringent crystals will be of great importance in commercial applications and materials science. A series of ultraviolet (UV) birefringent crystals, AX·(H
    Language English
    Publishing date 2024-01-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1484438-2
    ISSN 1520-510X ; 0020-1669
    ISSN (online) 1520-510X
    ISSN 0020-1669
    DOI 10.1021/acs.inorgchem.3c04371
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Analysis of the Research Hotspot of Exosomes in Cardiovascular Disease: A Bibliometric-based Literature Review.

    Cui, Jing / Li, Yiwen / Zhu, Mengmeng / Liu, Yanfei / Liu, Yue

    Current vascular pharmacology

    2023  Volume 21, Issue 5, Page(s) 316–345

    Abstract: Objective: To investigate the current status and development trend of research on exosomes in cardiovascular disease (CVD) using bibliometric analysis and to elucidate trending research topics.: Methods: Research articles on exosomes in CVD published ...

    Abstract Objective: To investigate the current status and development trend of research on exosomes in cardiovascular disease (CVD) using bibliometric analysis and to elucidate trending research topics.
    Methods: Research articles on exosomes in CVD published up to April 2022 were retrieved from the Web of Science database. Data were organized using Microsoft Office Excel 2019. CiteSpace 6.1 and VOSviewer 1.6.18 were used for bibliometric analysis and result visualization.
    Results: Overall, 256 original research publications containing 190 fundamental research publications and 66 clinical research publications were included. "Extracellular vesicle" was the most frequent research keyword, followed by "microrna," "apoptosis," and "angiogenesis." Most publications were from China (187, 73.05%), followed by the United States (57, 22.27%), the United Kingdom (7, 2.73%), and Japan (7, 2.73%). A systematic review of the publications revealed that myocardial infarction and stroke were the most popular topics and that exosomes and their contents, such as microRNAs (miRNAs), play positive roles in neuroprotection, inhibition of autophagy and apoptosis, promotion of angiogenesis, and protection of cardiomyocytes.
    Conclusion: Research on exosomes in CVD has attracted considerable attention, with China having the most published studies. Fundamental research has focused on CVD pathogenesis; exosomes regulate the progression of CVD through biological processes, such as the inflammatory response, autophagy, and apoptosis. Clinical research has focused on biomarkers for CVD; studies on using miRNAs in exosomes as disease markers for diagnosis could become a future trend.
    MeSH term(s) Humans ; Bibliometrics ; Cardiovascular Diseases/diagnosis ; Exosomes ; Extracellular Vesicles ; MicroRNAs/genetics
    Chemical Substances MicroRNAs
    Language English
    Publishing date 2023-09-15
    Publishing country United Arab Emirates
    Document type Journal Article ; Review
    ZDB-ID 2192362-0
    ISSN 1875-6212 ; 1570-1611
    ISSN (online) 1875-6212
    ISSN 1570-1611
    DOI 10.2174/0115701611249727230920042944
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Rhizosphere microbial community assembly and association networks strongly differ based on vegetation type at a local environment scale.

    Liu, Luxian / Ma, Liya / Zhu, Mengmeng / Liu, Bo / Liu, Xu / Shi, Yu

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1129471

    Abstract: Introduction: Rhizosphere microbes perform critical functions for their hosts, and their structure is strongly influenced by vegetation type. Although studies on the effects of vegetation on rhizosphere microbial community structure have been conducted ... ...

    Abstract Introduction: Rhizosphere microbes perform critical functions for their hosts, and their structure is strongly influenced by vegetation type. Although studies on the effects of vegetation on rhizosphere microbial community structure have been conducted at large and global environment scales, studies at local environment scales would eliminate numerous external factors such as climate and soil type, while highlighting the potential influence of local vegetation type.
    Methods: Here, we compared rhizosphere microbial communities using 54 samples under three vegetation types (herb, shrubs, and arbors, with bulk soil as the control) at the campus of Henan University. 16S rRNA and ITS amplicons were sequenced using Illumina high throughput sequencing.
    Results and discussion: Rhizosphere bacterial and fungal community structures were influenced considerably by vegetation type. Bacterial alpha diversity under herbs was significantly different from that under arbors and shrubs. The abundance of phyla such as Actinobacteria was extremely higher in bulk soil than in the rhizosphere soils. Herb rhizosphere harbored more unique species than other vegetation type soils. Furthermore, bacterial community assembly in bulk soil was more dominated by deterministic process, whereas the rhizosphere bacterial community assembly was dominated by stochasticity and the construction of fungal communities was all dominated by deterministic processes. In addition, rhizosphere microbial networks were less complex than bulk soil networks, and their keystone species differed based on vegetation type. Notably, bacterial community dissimilarities were strongly correlated with plant phylogenetic distance. Exploring rhizosphere microbial community patterns under different vegetation types could enhance our understanding of the role of rhizosphere microbes in ecosystem function and service provision, as well as basic information that could facilitate plant and microbial diversity conservation at the local environment scale.
    Language English
    Publishing date 2023-03-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1129471
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Diagnostic value of the cerebrospinal fluid lipoarabinomannan assay for tuberculous meningitis: a systematic review and meta-analysis.

    Chen, Ya-Li / Zhu, Meng-Meng / Guan, Cui-Ping / Zhang, Yan-An / Wang, Mao-Shui

    Frontiers in public health

    2023  Volume 11, Page(s) 1228134

    Abstract: Objective: This systematic review aims to evaluate the diagnostic accuracy of cerebrospinal fluid (CSF) lipoarabinomannan (LAM) assays in detecting tuberculous meningitis (TBM).: Methods: A systematic review search was conducted in PubMed and five ... ...

    Abstract Objective: This systematic review aims to evaluate the diagnostic accuracy of cerebrospinal fluid (CSF) lipoarabinomannan (LAM) assays in detecting tuberculous meningitis (TBM).
    Methods: A systematic review search was conducted in PubMed and five other databases up to April 2023. Studies that evaluated the diagnostic accuracy of CSF LAM assays were included with either definitive or composite reference standard used as the preferred reference standard. The quality of the included studies was assessed using the QUADAS-2 tool. We performed a bivariate random-effects meta-analysis and calculated the summary diagnostic statistics.
    Results: A total of six studies, including a sample size of 999, were included in the final analysis. The pooled sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) of CSF LAM for diagnosing TBM were determined to be 0.44 (95% CI: 0.31-0.58), 0.89 (95% CI: 0.81-0.93), and 0.76 (95% CI: 0.73-0.80), respectively. Significant heterogeneity was observed in both sensitivity (
    Conclusion: Although more prospective studies are required to validate the role of the CSF LAM assay, current evidence supports that the performance of the CSF LAM assay is unsatisfactory for the TBM diagnosis. Additionally, the optimization of the CSF LAM assay (e.g., improvements in CSF collection and preparation methods) should be considered to improve its performance.
    MeSH term(s) Humans ; Tuberculosis, Meningeal/diagnosis ; Tuberculosis, Meningeal/cerebrospinal fluid ; Prospective Studies ; Retrospective Studies ; Lipopolysaccharides/cerebrospinal fluid
    Chemical Substances lipoarabinomannan ; Lipopolysaccharides
    Language English
    Publishing date 2023-09-21
    Publishing country Switzerland
    Document type Meta-Analysis ; Systematic Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2711781-9
    ISSN 2296-2565 ; 2296-2565
    ISSN (online) 2296-2565
    ISSN 2296-2565
    DOI 10.3389/fpubh.2023.1228134
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Influence of inherent minerals on metalworking fluids sludge pyrolysis: Products characterization and heavy metals behavior.

    Zhu, Guidan / Wang, Xingdong / Yin, Xuan / Zhu, Mengmeng / Li, Jiaying / Cao, Ling / Sun, Zhiyang / Zeng, Hehua

    Heliyon

    2024  Volume 10, Issue 4, Page(s) e26256

    Abstract: Safely and appropriately disposing of metalworking fluids sludge (MFS) remains a challenge owing to its highly hazardous properties, this work investigated MFS pyrolysis at various temperatures (500, 600, 700, 800, and 900 °C) for energy recovery and ... ...

    Abstract Safely and appropriately disposing of metalworking fluids sludge (MFS) remains a challenge owing to its highly hazardous properties, this work investigated MFS pyrolysis at various temperatures (500, 600, 700, 800, and 900 °C) for energy recovery and safety treatment of MFS. The experimental results indicated that inherent minerals at higher temperatures could enhance the gas yields and promote the qualities of oil and gas from MFS pyrolysis. The highest pyrolysis gas yield was achieved at 18.86 wt% after MFS pyrolysis at 900 °C. GC-MS analysis revealed that the inherent minerals facilitated a decrease in oxygenated and nitrogenated compounds within the oil, while simultaneously leading to a substantial increase in hydrocarbon contents. Notably, the highest content of aromatics (61.16%) was attained during pyrolysis at 900 °C. Moreover, inherent minerals improved carbon sequestration and the characteristics of biochar during the MFS pyrolysis. The leaching contents of heavy metals in biochars were reduced, thereby reducing the heavy metals associated environmental risk. This research suggests that the pyrolysis process was a promising approach for simultaneous energy recovery and MFS disposal with low environmental risk.
    Language English
    Publishing date 2024-02-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e26256
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: MiPepid: MicroPeptide identification tool using machine learning.

    Zhu, Mengmeng / Gribskov, Michael

    BMC bioinformatics

    2019  Volume 20, Issue 1, Page(s) 559

    Abstract: Background: Micropeptides are small proteins with length < = 100 amino acids. Short open reading frames that could produces micropeptides were traditionally ignored due to technical difficulties, as few small peptides had been experimentally confirmed. ... ...

    Abstract Background: Micropeptides are small proteins with length < = 100 amino acids. Short open reading frames that could produces micropeptides were traditionally ignored due to technical difficulties, as few small peptides had been experimentally confirmed. In the past decade, a growing number of micropeptides have been shown to play significant roles in vital biological activities. Despite the increased amount of data, we still lack bioinformatics tools for specifically identifying micropeptides from DNA sequences. Indeed, most existing tools for classifying coding and noncoding ORFs were built on datasets in which "normal-sized" proteins were considered to be positives and short ORFs were generally considered to be noncoding. Since the functional and biophysical constraints on small peptides are likely to be different from those on "normal" proteins, methods for predicting short translated ORFs must be trained independently from those for longer proteins.
    Results: In this study, we have developed MiPepid, a machine-learning tool specifically for the identification of micropeptides. We trained MiPepid using carefully cleaned data from existing databases and used logistic regression with 4-mer features. With only the sequence information of an ORF, MiPepid is able to predict whether it encodes a micropeptide with 96% accuracy on a blind dataset of high-confidence micropeptides, and to correctly classify newly discovered micropeptides not included in either the training or the blind test data. Compared with state-of-the-art coding potential prediction methods, MiPepid performs exceptionally well, as other methods incorrectly classify most bona fide micropeptides as noncoding. MiPepid is alignment-free and runs sufficiently fast for genome-scale analyses. It is easy to use and is available at https://github.com/MindAI/MiPepid.
    Conclusions: MiPepid was developed to specifically predict micropeptides, a category of proteins with increasing significance, from DNA sequences. It shows evident advantages over existing coding potential prediction methods on micropeptide identification. It is ready to use and runs fast.
    MeSH term(s) Computational Biology/methods ; Databases, Protein ; Machine Learning ; Open Reading Frames/genetics ; Peptides/analysis ; Software
    Chemical Substances Peptides
    Language English
    Publishing date 2019-11-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041484-5
    ISSN 1471-2105 ; 1471-2105
    ISSN (online) 1471-2105
    ISSN 1471-2105
    DOI 10.1186/s12859-019-3033-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Study on the Effect and Mechanism of Huaji Jianpi Decoction on Simple Obesity.

    Zhu, Mengmeng / Zhang, Baixia / Zhang, Jinghui / Ge, Shaoqin / Liu, Meiyun

    Evidence-based complementary and alternative medicine : eCAM

    2022  Volume 2022, Page(s) 5494224

    Abstract: Background: As the major type of obesity in clinical, simple obesity has gained increasing attention in recent years. Depending on the etiology and pathogenesis of simple obesity and combined with clinical practice experience, Huaji Jianpi decoction ( ... ...

    Abstract Background: As the major type of obesity in clinical, simple obesity has gained increasing attention in recent years. Depending on the etiology and pathogenesis of simple obesity and combined with clinical practice experience, Huaji Jianpi decoction (HJJPD) was established to invigorate the spleen and eliminate dampness; however, the underlying molecular mechanism is yet unclear.
    Materials and methods: A simple obesity mouse model was established by feeding a high-fat diet to the animals, and the related indexes were analyzed. The mice were divided into the normal, positive control (orlistat), and HJJPD high-dose, medium-dose, and low-dose groups. After 6 weeks of administration, the curative effect of HJJPD was observed. Simple obesity is associated with leptin resistance. The leptin signal transduction pathways mainly include the JAK2-STAT3, AMPK-ACC, LepRb-IRS-PI3K-PDE3B-cAMP, and LepRb-SHP2-MAPKs (ERK1/2) pathways. Therefore, the networks of HJJPD acting on these four pathway-related targets were constructed using the network pharmacology method, and the key nodes were identified.
    Results: After 6 weeks of drug intervention, we found a good therapeutic effect of HJJPD on simple obesity in the mouse model. The biological network analysis showed that HJJPD plays a role in treating leptin resistance in simple obesity by acting on multiple targets in the JAK2-STAT3 pathway via various components. Also, HJJPD can improve leptin resistance in mice by enhancing the binding force of LEP and LEPRB and activating the LEP-mediated JAK2-STAT3 signaling pathway.
    Conclusion: In this study, animal experiments, network pharmacology, and molecular biology were combined to establish a mouse model of simple obesity, confirm the role of HJJPD in the treatment of simple obesity, and preliminarily reveal the related mechanism. Relevant research results will provide a basis for the treatment of simple obesity and the drug discovery.
    Language English
    Publishing date 2022-04-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2022/5494224
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Network pharmacology, molecular docking, and in vitro experimental verification of the mechanism of Guanxining in treating diabetic atherosclerosis.

    Wang, Wenting / Wang, Sutong / Li, Yiwen / Zhu, Mengmeng / Xu, Qian / Luo, Binyu / Liu, Yanfei / Liu, Yue

    Journal of ethnopharmacology

    2024  Volume 324, Page(s) 117792

    Abstract: Ethnopharmacological relevance: Guanxinning(GXN) tablet is a patented traditional Chinese medicine widely used to prevent and treat cardiovascular diseases. However, its potential mechanism and target in anti-diabetic atherosclerosis have not been ... ...

    Abstract Ethnopharmacological relevance: Guanxinning(GXN) tablet is a patented traditional Chinese medicine widely used to prevent and treat cardiovascular diseases. However, its potential mechanism and target in anti-diabetic atherosclerosis have not been clarified.
    Aim: The aim of this study was to investigate the underlying targets and mechanisms of action GXN in the treatment of diabetic atherosclerosis, employing a combination of network pharmacology, molecular docking, and in vitro experimental verification.
    Methods: We predicted the core components and targets of GXN in the treatment of diabetic atherosclerosis through various databases, and made analysis and molecular docking. In vitro, we induced injury in human umbilical vein endothelial cells using glucose/palmitate and observed the effects of GXN on cellular damage high-glucose and high-fat conditions, subsequently elucidating its molecular mechanisms.
    Results: A total of 14 active components and 157 targets of GXN were identified. Using the PPI network, we selected 9 core active components and 20 targets of GXN. GO functional analysis revealed that these targets were primarily associated with apoptosis signaling pathways in response to endoplasmic reticulum stress and reactive oxygen species responses. Molecular docking confirmed the strong binding affinities of the primary active components of GXN with ERN1, MAPK1 and BECN1. In vitro experiments demonstrated the ability of GXN to restore endothelial cell activity, enhance cell migration and inhibit sICAM secretion, and upregulate the expression of endoplasmic reticulum stress-related proteins (IRE1, XBP1) and autophagy-related proteins (Beclin1, LC3A, and LC3B), while simultaneously inhibiting endothelial cell apoptosis under high-glucose and high-fat conditions.
    Conclusions: Our findings suggest that GXN can potentially safeguard endothelial cells from the adverse effects of high-glucose and high-fat by modulating the interactions between endoplasmic reticulum stress and autophagy. Therefore, GXN is a promising candidate for the prevention and treatment of diabetic atherosclerosis.
    MeSH term(s) Humans ; Molecular Docking Simulation ; Network Pharmacology ; Atherosclerosis/drug therapy ; Glucose ; Human Umbilical Vein Endothelial Cells ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Diabetes Mellitus/drug therapy
    Chemical Substances Glucose (IY9XDZ35W2) ; Drugs, Chinese Herbal
    Language English
    Publishing date 2024-01-28
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2024.117792
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Two novel GCK mutations in Chinese patients with maturity-onset diabetes of the young.

    Wang, Tao / Zhu, Mengmeng / Wang, Yun / Hu, Cheng / Fang, Chen / Hu, Ji

    Endocrine

    2023  Volume 83, Issue 1, Page(s) 92–98

    Abstract: Purpose: Heterozygous inactivating mutations in the glucokinase (GCK) gene result in the asymptomatic fasting hyperglycemia named as GCK-MODY or MODY2. The genetic testing can effectively avoid the misdiagnosis and inappropriate treatment for GCK-MODY.!# ...

    Abstract Purpose: Heterozygous inactivating mutations in the glucokinase (GCK) gene result in the asymptomatic fasting hyperglycemia named as GCK-MODY or MODY2. The genetic testing can effectively avoid the misdiagnosis and inappropriate treatment for GCK-MODY.
    Methods: A total of 25 unrelated families with MODY were screened for mutations in coding region of GCK by using direct sequencing. Three different bioinformatics tools such as PolyPhen2, Mutation Taster and PROVEAN were performed to predict the function of mutant proteins. The glucose profile was recorded by continuous glucose monitoring system (CGMS) to evaluate the glycemic variability for the GCK-MODY patient.
    Results: Our study identified five GCK mutations in 24% of the families (6/25): two novel mutations (I126fs and G385A) and three already described mutations (G44S, H50fs and S383L). In silico analyses predicted that these mutations altered structural conformational changes. The values of mean amplitude of glycemic excursions (MAGE), an important index of blood glucose fluctuation in CGMS system, were 0.81 in the first 24 h and 1.61 in the second 24 h record in the patient with GCK-MODY (F3), suggesting little glucose fluctuation.
    Conclusion: The genetic testing is suggested to be important to differentiate GCK-MODY from other types of diabetes. CGMS might be used to screen GCK-MODY cases prior to genetic testing.
    MeSH term(s) Humans ; Blood Glucose ; Blood Glucose Self-Monitoring ; China ; Diabetes Mellitus, Type 2/genetics ; Diabetes Mellitus, Type 2/diagnosis ; Glucokinase/genetics ; Glucose ; Mutation
    Chemical Substances Blood Glucose ; Glucokinase (EC 2.7.1.2) ; Glucose (IY9XDZ35W2) ; MAP4K2 protein, human
    Language English
    Publishing date 2023-10-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1194484-5
    ISSN 1559-0100 ; 1355-008X ; 0969-711X
    ISSN (online) 1559-0100
    ISSN 1355-008X ; 0969-711X
    DOI 10.1007/s12020-023-03509-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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