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  1. Article ; Online: Ordered Porous Layer Interferometry for Dynamic Observation of Non-Specific Adsorption Induced by 1-Ethyl-3-(3-(dimethylamino)propyl) Carbodiimide.

    Zhang, Yu / Wang, Lu / Ma, Ning / Wan, Yizhen / Zhu, Xueyi / Qian, Weiping

    Langmuir : the ACS journal of surfaces and colloids

    2023  Volume 39, Issue 32, Page(s) 11406–11413

    Abstract: Nonspecific adsorption (NSA) seems to be an impregnable obstacle to the progress of the biomedical, diagnostic, microelectronic, and material fields. The reaction path of bioconjugation can alter the surface charge distribution on products and the ... ...

    Abstract Nonspecific adsorption (NSA) seems to be an impregnable obstacle to the progress of the biomedical, diagnostic, microelectronic, and material fields. The reaction path of bioconjugation can alter the surface charge distribution on products and the interaction of bioconjugates, an ignored factor causing NSA. We monitored exacerbated NSA introduced by a 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC) addition reaction, which cannot be resistant to bovine serum albumin (BSA) or polyethylene glycol (PEG) antifouling coating and Tween-20. And the negative effects can be minimized by adding as low as 7.5 × 10
    Language English
    Publishing date 2023-08-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2005937-1
    ISSN 1520-5827 ; 0743-7463
    ISSN (online) 1520-5827
    ISSN 0743-7463
    DOI 10.1021/acs.langmuir.3c01266
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Evaluation of covalent coupling strategies for immobilizing ligands on silica colloidal crystal films by optical interferometry.

    Zhu, Xueyi / Ma, Ning / Wan, Yizhen / Wang, Lu / Zhang, Yu / Yan, Chengyu / Qian, Weiping

    The Analyst

    2023  Volume 148, Issue 5, Page(s) 1024–1031

    Abstract: Immobilizing ligands is a crucial part of preparing optical sensors and directly connected to the sensitivity, stability, and other characteristics of sensors. In this work, an ordered porous layer interferometry (OPLI) system that can monitor the ... ...

    Abstract Immobilizing ligands is a crucial part of preparing optical sensors and directly connected to the sensitivity, stability, and other characteristics of sensors. In this work, an ordered porous layer interferometry (OPLI) system that can monitor the covalent coupling process of ligands in real time was developed. Films of silica colloidal crystal (SCC), as optical interference substrates, were surface modified by three different reagents: chloroacetic acid, glutaric anhydride, and carboxymethyl dextran.
    Language English
    Publishing date 2023-02-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d2an02079c
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2423: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  3. Article: Long Noncoding RNAs in the Regulation of Asthma: Current Research and Clinical Implications.

    Zhu, Xueyi / Wei, Ying / Dong, Jingcheng

    Frontiers in pharmacology

    2020  Volume 11, Page(s) 532849

    Abstract: Asthma is a chronic airway inflammatory disorder related to variable expiratory airflow limitation, leading to wheeze, shortness of breath, chest tightness, and cough. Its characteristic features include airway inflammation, airway remodeling and airway ... ...

    Abstract Asthma is a chronic airway inflammatory disorder related to variable expiratory airflow limitation, leading to wheeze, shortness of breath, chest tightness, and cough. Its characteristic features include airway inflammation, airway remodeling and airway hyperresponsiveness. The pathogenesis of asthma remains extremely complicated and the detailed mechanisms are not clarified. Long noncoding RNAs (lncRNAs) have been reported to play a prominent role in asthma and function as modulators of various aspects in pathological progress of asthma. Here, we summarize recent advances of lncRNAs in asthma pathogenesis to guide future researches, clinical treatment and drug development, including their regulatory functions in the T helper (Th) 1/Th2 imbalance, Th17/T regulatory (Treg) imbalance, eosinophils dysfunction, macrophage polarization, airway smooth muscle cells proliferation, and glucocorticoid insensitivity.
    Language English
    Publishing date 2020-09-11
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2020.532849
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  4. Article ; Online: Monitoring of silica colloidal crystal-embedded chitosan hydrogel films swelling and its drug release application.

    Yan, Chengyu / Wang, Lu / Ma, Ning / Wan, Yizhen / Zhou, Lele / Zhu, Xueyi / Qian, Weiping

    Analytica chimica acta

    2022  Volume 1236, Page(s) 340582

    Abstract: The hydrogels, because of their swelling properties in response to the environmental stimulus, are being widely considered for the design of controlled drug release systems. To meet the need for developing effective drug delivery methods, we developed ... ...

    Abstract The hydrogels, because of their swelling properties in response to the environmental stimulus, are being widely considered for the design of controlled drug release systems. To meet the need for developing effective drug delivery methods, we developed special silica colloidal crystal (SCC)-embedded chitosan hydrogel films. The SCC films served as an interference substrate and drug storage layer, while the chitosan hydrogel served as a cover to regulate the drug release. The optical interferometry was performed to dynamically monitor the volume phase transition of chitosan hydrogel response to pH stimulation. Furthermore, the effects of crosslinking ratio and hydrogel thickness on the swelling properties of chitosan hydrogel were also evaluated. More importantly, the pH-responsive swelling of chitosan hydrogel was used to slowly release indomethacin. This system may provide support for drug delivery studies, therefore further expected to apply in the enhancement of the treatment efficiency of new drug therapies.
    MeSH term(s) Hydrogels/chemistry ; Chitosan/chemistry ; Drug Liberation ; Silicon Dioxide ; Hydrogen-Ion Concentration
    Chemical Substances Hydrogels ; Chitosan (9012-76-4) ; hydrogel film ; Silicon Dioxide (7631-86-9)
    Language English
    Publishing date 2022-11-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2022.340582
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  5. Article ; Online: Condensed tannin accretions specifically distributed in mesophyll cells of non-salt secretor mangroves help in salt tolerance.

    Zhu, Xueyi / Huang, Hezi / Luo, Xu / Wei, Yuanhai / Du, Shuangling / Yu, Jiamin / Guo, Shengyu / Chen, Kaiyun / Chen, Linjiao

    Planta

    2023  Volume 258, Issue 5, Page(s) 100

    Abstract: Main conclusion: Auto-fluorescent condensed tannins specifically accumulated in mesophyll cells of non-salt secretor mangroves are involved in the compartmentation of ... ...

    Abstract Main conclusion: Auto-fluorescent condensed tannins specifically accumulated in mesophyll cells of non-salt secretor mangroves are involved in the compartmentation of Na
    MeSH term(s) Mesophyll Cells ; Proanthocyanidins ; Salt Tolerance ; Sodium Chloride/pharmacology ; Plant Leaves/physiology ; Salinity
    Chemical Substances Proanthocyanidins ; Sodium Chloride (451W47IQ8X)
    Language English
    Publishing date 2023-10-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/s00425-023-04254-5
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    In stock of ZB MED Bonn / Germany

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  6. Article ; Online: Metalens in harmony with refractive optics.

    Zhu, Xueyi / Wang, Shuming / Yan, Xuejun / Lu, Minghui

    Science bulletin

    2019  Volume 64, Issue 12, Page(s) 797–798

    Language English
    Publishing date 2019-04-04
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2816140-3
    ISSN 2095-9281 ; 2095-9273
    ISSN (online) 2095-9281
    ISSN 2095-9273
    DOI 10.1016/j.scib.2019.04.010
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  7. Article ; Online: Understory and canopy additions of nitrogen differentially affect carbon and nitrogen metabolism of Psychotria rubra in an evergreen broad-leaved forest.

    Liu, Nan / Zhang, Shike / Huang, Yao / Cai, Hongyue / Zhu, Xueyi

    The Science of the total environment

    2020  Volume 724, Page(s) 138183

    Abstract: To assess the effects of nitrogen deposition on forest plants, researchers have conducted many nitrogen-addition experiments. Most of these experiments, however, failed to fully simulate atmospheric nitrogen deposition because they failed to assess ... ...

    Abstract To assess the effects of nitrogen deposition on forest plants, researchers have conducted many nitrogen-addition experiments. Most of these experiments, however, failed to fully simulate atmospheric nitrogen deposition because they failed to assess interception of nitrogen deposition by the forest canopy. Here, we used transcriptomics, proteomics, and metabolomics to compare the effects of understory nitrogen addition (UAN), canopy nitrogen addition (CAN), and a control (no nitrogen addition) on carbon and nitrogen metabolism in leaves of Psychotria rubra, a dominant subtropical understory plant species in evergreen broad-leaved forests in South China. We first established a reference P. rubra transcriptome via RNA-seq and obtained a total of 93,986 unigenes from de novo assembly. Next, we quantitatively investigated the proteome and metabolome in leaves, and identified 4021 proteins and 562 metabolites. Under the CAN treatment and relative to the control, 36 genes were up-regulated and 23 were down-regulated, and we identified 46 up-regulated proteins and 49 down-regulated proteins. Under the UAN treatment and relative to the control, 1525 genes were up-regulated and 224 genes down-regulated, and we identified 35 up-regulated proteins and 71 down-regulated proteins. These differentially expressed genes and proteins were related to photosynthesis, amino acid metabolism, and flavonoid biosynthesis. Accordingly, 15 differentially accumulated metabolites in response to CAN and 20 in response to UAN were found; these differentially accumulated metabolites included 4 amino acids and 3 flavonoids. Taken together, our results show that the UAN treatment had a greater effect on photosynthesis, amino acid metabolism, and flavonoid biosynthesis than the CAN treatment. Most importantly, the results indicate that understory application of nitrogen in experiments may incorrectly estimate the effects of nitrogen deposition on nitrogen and carbon utilization by P. rubra and perhaps by other understory woody plants in the evergreen broad-leaved forests in South China as well.
    MeSH term(s) Carbon ; China ; Forests ; Nitrogen ; Psychotria ; Trees
    Chemical Substances Carbon (7440-44-0) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2020-03-25
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2020.138183
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 949: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: Physiological and Proteomic Analyses of Different Ecotypes of Reed (

    Li, Huan / Lin, Wen-Fang / Shen, Zhi-Jun / Peng, Hao / Zhou, Jia-Jie / Zhu, Xue-Yi

    Frontiers in plant science

    2021  Volume 12, Page(s) 720593

    Abstract: Drought and salinity are the two major abiotic stresses constraining the crop yield worldwide. Both of them trigger cellular dehydration and cause osmotic stress which leads to cytosolic and vacuolar volume reduction. However, whether plants share a ... ...

    Abstract Drought and salinity are the two major abiotic stresses constraining the crop yield worldwide. Both of them trigger cellular dehydration and cause osmotic stress which leads to cytosolic and vacuolar volume reduction. However, whether plants share a similar tolerance mechanism in response to these two stresses under natural conditions has seldom been comparatively reported. There are three different ecotypes of reed within a 5 km
    Language English
    Publishing date 2021-09-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2021.720593
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  9. Article: Screening and Verification of Differentially Expressed Long Non-coding RNAs in the Peripheral Blood of Patients With asthma.

    Ma, Cheng / Wang, Shiyuan / Cao, Yuxue / Tang, Weifeng / Wuniqiemu, Tulake / Teng, Fangzhou / Zhu, Xueyi / Wei, Ying / Dong, Jingcheng

    Frontiers in pharmacology

    2022  Volume 13, Page(s) 834009

    Abstract: Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in the pathogenesis of asthma. Although some differentially expressed lncRNAs have been identified in asthmatic patients, many asthma-related lncRNAs have not been annotated. ... ...

    Abstract Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in the pathogenesis of asthma. Although some differentially expressed lncRNAs have been identified in asthmatic patients, many asthma-related lncRNAs have not been annotated. In the present study, six patients and three healthy subjects were randomly selected from 34 asthmatic patients and 17 healthy subjects. Second-generation high-throughput sequencing was performed on their peripheral blood samples. There were 1,137 differentially expressed lncRNAs in the asthma patients compared to in the healthy controls, of which 485 were upregulated and 652 were downregulated. The top 30 enriched GO and KEGG terms were identified, and the cytosolic ribosome (GO:0022626) and ribosome (hsa03010) were associated with the most differentially expressed lncRNAs. The top 10 differentially expressed lncRNAs associated with asthma were verified by an lncRNA-mRNA co-expression network and RT-qPCR. Seven of the these (NONHSAT015495.2, MSTRG.71212.2, NONHSAT163272.1, NONHSAT181891.1, NONHSAT190964.1, ENST00000564809, and NONHSAT076890.2) were down-regulated in the peripheral blood of asthmatic patients, which was consistent with the sequencing results. Three patients and three healthy subjects were randomly selected from the remaining subjects to verify these seven lncRNAs by RT-qPCR, which further confirmed the sequencing results. Public database GSE106230 was also in agreement with the FPKM (Fragments Per kilobase of exon model per Million mapped reads) trends of ENST00000564809, NONHSAT015495.2, NONHSAT181891.1, and NONHSAT190964.1. In conclusion, the present study identified seven lncRNAs that may serve as potential biological markers for asthma.
    Language English
    Publishing date 2022-02-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2022.834009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Understory and canopy additions of nitrogen differentially affect carbon and nitrogen metabolism of Psychotria rubra in an evergreen broad-leaved forest

    Liu, Nan / Zhang, Shike / Huang, Yao / Cai, Hongyue / Zhu, Xueyi

    Science of the total environment. 2020 July 01, v. 724

    2020  

    Abstract: To assess the effects of nitrogen deposition on forest plants, researchers have conducted many nitrogen-addition experiments. Most of these experiments, however, failed to fully simulate atmospheric nitrogen deposition because they failed to assess ... ...

    Abstract To assess the effects of nitrogen deposition on forest plants, researchers have conducted many nitrogen-addition experiments. Most of these experiments, however, failed to fully simulate atmospheric nitrogen deposition because they failed to assess interception of nitrogen deposition by the forest canopy. Here, we used transcriptomics, proteomics, and metabolomics to compare the effects of understory nitrogen addition (UAN), canopy nitrogen addition (CAN), and a control (no nitrogen addition) on carbon and nitrogen metabolism in leaves of Psychotria rubra, a dominant subtropical understory plant species in evergreen broad-leaved forests in South China. We first established a reference P. rubra transcriptome via RNA-seq and obtained a total of 93,986 unigenes from de novo assembly. Next, we quantitatively investigated the proteome and metabolome in leaves, and identified 4021 proteins and 562 metabolites. Under the CAN treatment and relative to the control, 36 genes were up-regulated and 23 were down-regulated, and we identified 46 up-regulated proteins and 49 down-regulated proteins. Under the UAN treatment and relative to the control, 1525 genes were up-regulated and 224 genes down-regulated, and we identified 35 up-regulated proteins and 71 down-regulated proteins. These differentially expressed genes and proteins were related to photosynthesis, amino acid metabolism, and flavonoid biosynthesis. Accordingly, 15 differentially accumulated metabolites in response to CAN and 20 in response to UAN were found; these differentially accumulated metabolites included 4 amino acids and 3 flavonoids. Taken together, our results show that the UAN treatment had a greater effect on photosynthesis, amino acid metabolism, and flavonoid biosynthesis than the CAN treatment. Most importantly, the results indicate that understory application of nitrogen in experiments may incorrectly estimate the effects of nitrogen deposition on nitrogen and carbon utilization by P. rubra and perhaps by other understory woody plants in the evergreen broad-leaved forests in South China as well.
    Keywords Psychotria ; amino acid metabolism ; amino acids ; biosynthesis ; broadleaved evergreen forests ; carbon ; deciduous forests ; flavonoids ; forest canopy ; forest plants ; gene expression regulation ; leaves ; metabolites ; metabolome ; metabolomics ; nitrogen ; nitrogen metabolism ; photosynthesis ; proteins ; proteome ; proteomics ; sequence analysis ; transcriptome ; transcriptomics ; understory ; unigenes ; woody plants ; China
    Language English
    Dates of publication 2020-0701
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2020.138183
    Database NAL-Catalogue (AGRICOLA)

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