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  1. Article: [Effects of simvastatin on the proliferation and apoptosis of prostatic epithelial RWPE-1 cells].

    Yang, Ming-gen / Zheng, Zhou-da / Lin, Hai-li / Zhuang, Zhi-ming / Lin, Tian-qi

    Zhonghua nan ke xue = National journal of andrology

    2015  Volume 21, Issue 2, Page(s) 113–118

    Abstract: Objective: To investigate the effects of simvastatin on the proliferation and apoptosis of prostatic epithelial RWPE-1 cells.: Methods: RWPE-1 cells cultured in vitro were treated with simvastatin at 0, 10, 20, and 40 μmol/L for 24, 48, and 72 hours ... ...

    Abstract Objective: To investigate the effects of simvastatin on the proliferation and apoptosis of prostatic epithelial RWPE-1 cells.
    Methods: RWPE-1 cells cultured in vitro were treated with simvastatin at 0, 10, 20, and 40 μmol/L for 24, 48, and 72 hours followed by determination of their proliferation by MTT assay, and their apoptosis by flow cytometry. The mRNA and protein expressions of Bcl-2, Bax, and Cx43 were detected by fluorescence quantitative RT-PCR and Western blot, respectively.
    Results: After 72 hours of treatment with simvastatin at 10, 20, and 40 μmol/L, the inhibition rates of the RWPE-1 cells were (21.07 ± 6.41)%, (34.87 ± 9.65)%, and (47.18 ± 10.88)%, respectively, significantly higher than (1.21 ± 0.54)% in the control group (P < 0.05) and in a dose-dependent manner (P < 0.05); the cell apoptosis rates were (0.066 ± 0.016)%, (0.126 ± 0.023)%, and (0.192 ± 0.025)%, respectively, remarkably higher than (0.015 ± 0.005)% in the control (P < 0.05) and also in a dose-dependent manner (P < 0.05); the mRNA and protein expressions of Bcl-2 were decreasing while those of Bax and Cx43 increasing with the increased concentration of simvastatin (P < 0.05). The expression of Cx43 was correlated negatively with that of Bcl-2 but positively with that of Bax.
    Conclusion: Simvastatin inhibits the proliferation of prostate epithelial cells and induce their apoptosis by acting on the gap junctional intercellular communication.
    MeSH term(s) Apoptosis/drug effects ; Cell Proliferation/drug effects ; Connexin 43/metabolism ; Drug Administration Schedule ; Epithelial Cells/drug effects ; Epithelial Cells/physiology ; Humans ; Hypolipidemic Agents/pharmacology ; Male ; Prostate/cytology ; Proto-Oncogene Proteins c-bcl-2/metabolism ; RNA, Messenger/metabolism ; Simvastatin/pharmacology ; bcl-2-Associated X Protein/metabolism
    Chemical Substances BAX protein, human ; Connexin 43 ; Hypolipidemic Agents ; Proto-Oncogene Proteins c-bcl-2 ; RNA, Messenger ; bcl-2-Associated X Protein ; Simvastatin (AGG2FN16EV)
    Language Chinese
    Publishing date 2015-02
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267480-9
    ISSN 1009-3591
    ISSN 1009-3591
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6577: Show issues Location:
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  2. Article: [Oral medication of statins retards the progression of benign prostatic hyperplasia and lower urinary tract symptoms].

    Yang, Ming-Gen / Zheng, Zhou-Da / Lin, Hai-Li / Zhuang, Zhi-Ming / Lin, Tian-Qi

    Zhonghua nan ke xue = National journal of andrology

    2014  Volume 20, Issue 9, Page(s) 798–802

    Abstract: Objective: To determine whether oral statins can delay the progression of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS).: Methods: We conducted a retrospective cohort study of 50-69-year-old males who came for physical ... ...

    Abstract Objective: To determine whether oral statins can delay the progression of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS).
    Methods: We conducted a retrospective cohort study of 50-69-year-old males who came for physical examination in our hospital between January 2003 and December 2008. We designed the inclusion criteria, followed them up for 5 years, and investigated the relationship of oral statins with the clinical progression of BPH and LUTS.
    Results: Totally, 653 men met the inclusion criteria and were included in this study, of whom 283 were treated with oral statins (group 1) while the other 370 with none (group 2). There were no statistically significant differences between the two groups in age and baseline IPSS, Qmax, and prostate volume (PV) (P > 0.05). During the follow-up, 24 cases in group 1 and 35 cases in group 2 were excluded for obvious dys-uria. A gradual increase was observed in IPSS in both groups 1 and 2 year by year from the baseline to the 5th year of follow-up, but significantly lower in the former group (4.27 +/- 1.16, 4.63 +/- 1.05, 5.27 +/- 0.96, 6.41 +/- 1.04, 7.21 +/- 1.21, and 7.93 +/-1.50) than in the latter (4.24 +/- 1.35, 5.26 +/- 1.23, 6.84 +/- 1.20, 8.75 +/- 1.84, 10.82 +/- 3.01, and 12.98 +/- 4.21) (P < 0.01); a gradual decrease was seen in Qmax, though markedly higher in group 1 ([26.56 +/- 2.09], [24.06 +/- 1.94], [21.33 +/- 1.66], [19.24 +/- 1.54], [17.44 +/- 1.53], and [16.27 +/- 1.37] ml/s) than in group 2 ([26.74 +/- 2.40], [23.62 +/- 2.01], [20.63 +/- 1.69], [17.72 +/- 1.48], [14.82 +/- 1.11], and [11.86 +/- 1.24] ml/s) (P < 0.01); and a gradual increase was found in PV, but remarkably smaller in the former group ([19.82 +/- 4.94], [22.60 +/- 4.99], [25.80 +/- 5.20], [27.92 +/- 5.05], [29.11 +/- 5.24], and [29.97 +/- 5.26] ml) than in the latter ([20.21 +/- 4.78], [24.30 +/- 4.98], [28.50 +/- 5.14], [32.84 +/- 4.77], [36.99 +/- 4.78], and [40.90 +/- 4.78] ml) (P < 0.01). Longer medication of statins was associated with better efficacy.
    Conclusion: Oral statins can significantly delay the clinical progression of BPH and LUTS.
    MeSH term(s) Aged ; Humans ; Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use ; Longitudinal Studies ; Lower Urinary Tract Symptoms/drug therapy ; Male ; Middle Aged ; Prostatic Hyperplasia/drug therapy ; Retrospective Studies
    Chemical Substances Hydroxymethylglutaryl-CoA Reductase Inhibitors
    Language Chinese
    Publishing date 2014-09
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267480-9
    ISSN 1009-3591
    ISSN 1009-3591
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article: [Qilin Pills combined with clomiphene for idiopathic oligoasthenozoospermia].

    Yang, Ming-gen / Zheng, Gou-da / Xu, Zhen-qiang / Lin, Hai-li / Zhuang, Zhi-ming / Zhang, Chao-xian

    Zhonghua nan ke xue = National journal of andrology

    2015  Volume 21, Issue 6, Page(s) 549–554

    Abstract: Objective: To observe the therapeutic effect of Qilin Pills combined with clomiphene on idiopathic oligoasthenospermia.: Methods: We randomly assigned 300 patients with idiopathic oligoasthenospermia to a trial (n = 156) and a control group (n = 144) ...

    Abstract Objective: To observe the therapeutic effect of Qilin Pills combined with clomiphene on idiopathic oligoasthenospermia.
    Methods: We randomly assigned 300 patients with idiopathic oligoasthenospermia to a trial (n = 156) and a control group (n = 144) to be treated with Qilin Pills (6 g, tid) combined with clomiphene (50 mg, qd) and clomiphene alone (50 mg, qd), respectively, both for a course of 12 weeks. Before and after 4, 8, and 12 weeks of medication, we determined sperm concentration, the percentages of grade a and grade a + b sperm, sperm motility, and the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T), followed by evaluation of the clinical efficacy of Qilin Pills with the pregnancy rate in the patients' spouses as the secondaty therapeutic indexes.
    Results: Compared with the baseline, both groups of patients showed remarkably improved semen parameters and hormone levels after treatment (all P < 0.01). After 4, 8, and 12 weeks of medication, statistically significant differences were observed between the trial and control groups in sperm concentration ([17.06 ± 2.24] vs [15.07 ± 2.48], [22.10 ± 2.65] vs [18.11 ± 2.97], and [28.13 ± 3.59] vs [21.21 ± 3.60] x 10(6)/mL, P < 0.01), the percentage of grade a sperm ([15.03 ± 2.39] vs [13.08 ± 2.51], [21.08 ± 3.16] vs [16.04 ± 3.05], and [28.08 ± 4.70] vs [20.14 ± 4.74]%, P < 0.01), the percentage of grade a + b sperm ([30.10 ± 5.07] vs [26.21 ± 3.96], [38.08 ± 5.64] vs [30.07 ± 4.80], and [48.04 ± 6.49] vs [35.28 ± 4.77]%, P < 0.01), sperm motility ([42.04 ± 4.86] vs [40.29 ± 4.19], [52.05 ± 5.58] vs [48.03 ± 4.40], and [65.03 ± 5.13] vs [56.67 ± 4.99]%), the FSH level ([7.75 ± 1.38] vs [7.20 ± 1.17], [10.83 ± 1.23] vs [9.10 ± 1.32], and [14.22 ± 0.84] vs [12.06 ± 1.45] IU/L, P < 0.01), the LH level ([10.05 ± 1.68] vs [9.18 ± 1.54], [13.96 ± 1.68] vs [11.99 ± 1.71], and [19.01 ± 2.42] vs [15.86 ± 2.08] IU/L, P < 0.01) and the T level ([19.19 ± 192] vs [18.34 ± 1.79] [21.06 ± 1.63] vs [20.06 ± 1.56], and [24.63 ± 1.06] vs [22.03 ± 1.49] nmol/L, P < 0.01). The pregnancy rate in the patients' spouses was significantly higher in the trial than in the control group at 4, 8, and 12 weeks (1.92 vs 0.69, 4.81 vs 3.47, and 11.54 vs 8.33%, P < 0.01). There were no statistically significant differences in drug tolerance between the two groups (P > 0.05). No obvious adverse reactions were observed.
    Conclusion: Qilin Pills combined with clomiphene can evidently improve the seminal quality and hormone level of oligoasthenospermia patients with no obvious adverse events. However, its long-term efficacy and tolerance deserve further clinical investigation.
    MeSH term(s) Asthenozoospermia/blood ; Asthenozoospermia/drug therapy ; Clomiphene/therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal/therapeutic use ; Female ; Fertility Agents/therapeutic use ; Follicle Stimulating Hormone/blood ; Humans ; Luteinizing Hormone/blood ; Male ; Pregnancy ; Pregnancy Rate ; Semen ; Sperm Count ; Sperm Motility ; Spermatozoa ; Testosterone/blood
    Chemical Substances Drugs, Chinese Herbal ; Fertility Agents ; Clomiphene (1HRS458QU2) ; Testosterone (3XMK78S47O) ; Luteinizing Hormone (9002-67-9) ; Follicle Stimulating Hormone (9002-68-0)
    Language Chinese
    Publishing date 2015-06
    Publishing country China
    Document type English Abstract ; Journal Article ; Randomized Controlled Trial
    ZDB-ID 2267480-9
    ISSN 1009-3591
    ISSN 1009-3591
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: [Prophylatic antibiotic use in percutaneous nephrolithotomy: a meta-analysis].

    Yang, Ming-gen / Zheng, Zhou-da / Xu, Zhen-qiang / Lin, Hai-li / Zhuang, Zhi-ming / Zhang, Chao-xian

    Zhonghua wai ke za zhi [Chinese journal of surgery

    2013  Volume 51, Issue 10, Page(s) 922–927

    Abstract: Objective: To determine whether antibiotic prophylaxis can reduce the risk of postoperative infective complications in patients undergoing percutaneous nephrolithotomy (PCNL) who have sterile preoperative urine.: Methods: MEDLINE, EMBASE, Cochrane ... ...

    Abstract Objective: To determine whether antibiotic prophylaxis can reduce the risk of postoperative infective complications in patients undergoing percutaneous nephrolithotomy (PCNL) who have sterile preoperative urine.
    Methods: MEDLINE, EMBASE, Cochrane Collaboration Reviews, CMCC and CNKI were searched for RCTs comparing antibiotic prophylaxis with placebo (or blank controls) for patients undergoing PCNL with preoperative sterile urine. The search strategy was made according to the Collaborative Review Group search strategy. Data were extracted by 2 reviewers using the designed extraction form. The software RevMan 4.2 was used to review management and data analysis.
    Results: A total of 9 trails, 1 placebo controlled, 3 non treatment controlled, and 5 active controlled, involving 1018 patients, met the inclusion criteria. Prophylactic antibiotic use in patients at low risk undergoing PCNL significantly decreased fever (RR = 0.71, 95% CI: 0.54-0.92, P = 0.009), bacteriuria (RR = 0.39, 95%CI: 0.23-0.67, P = 0.0006) and bacteremia incidence (RR = 0.43, 95%CI: 0.25-0.73, P = 0.002). Effective antibiotic classes included quinolone which significantly decreased bacteriuria incidence (RR = 0.31, 95%CI: 0.12-0.82, P = 0.010) and nitrofurantoin which significantly decreased fever incidence (RR = 0.38, 95%CI: 0.24-0.61, P = 0.005). Extended course significantly decreased fever incidence (RR = 0.64, 95%CI: 0.47-0.87, P = 0.004) and bacteriuria incidence (RR = 0.35, 95%CI: 0.18-0.71, P = 0.003).
    Conclusions: Prophylactic antibiotics can significantly decrease the incidence of postoperative infective complications. A significant decrease in bacteriuria incidence can be achieved with quinolones. Extended course is effective in decreasing fever, and bacteriuria incidence.
    MeSH term(s) Anti-Bacterial Agents/therapeutic use ; Antibiotic Prophylaxis ; Bacteriuria/prevention & control ; Humans ; Nephrostomy, Percutaneous ; Postoperative Complications/prevention & control
    Chemical Substances Anti-Bacterial Agents
    Language Chinese
    Publishing date 2013-10
    Publishing country China
    Document type English Abstract ; Journal Article ; Meta-Analysis ; Research Support, Non-U.S. Gov't
    ZDB-ID 604573-x
    ISSN 0529-5815
    ISSN 0529-5815
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 218: Show issues

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