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Article ; Online: Considering the Effect of Non-Propagating Cracks on Fatigue Limit Prediction in the Critical Distance Method Framework

Zhuo Zhou / Deqing Guan

Applied Sciences, Vol 12, Iss 10994, p

2022 Volume 10994

Abstract: Although the material has developed micro-scale cracks, the micro-cracks stop propagating and transform into non-propagating cracks (NPCs) under fatigue limit loading. The movement of the crack tip position caused by the non-propagating crack will ... ...

Abstract	Although the material has developed micro-scale cracks, the micro-cracks stop propagating and transform into non-propagating cracks (NPCs) under fatigue limit loading. The movement of the crack tip position caused by the non-propagating crack will generate a small change in the notch geometry, which easily triggers the geometric size effect. Since the critical distance method focuses on evaluating the limit of fatigue in terms of the material’s cracking conditions, the present study attempted to develop a notched fatigue analysis model to consider the effect of non-propagating cracks on fatigue limit prediction in the critical distance method framework. The effectiveness and capability of the proposed model were demonstrated by the fatigue experimental data of Q345qD low carbon steel.
Keywords	notch fatigue ; critical distance method ; non-propagating crack ; size effect ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	669
Language	English
Publishing date	2022-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Screening for inhibitors against SARS-CoV-2 and its variants

Weijing Yuan / Xiaojing Dong / Lan Chen / Xiaobo Lei / Zhuo Zhou / Li Guo / Jianwei Wang

Biosafety and Health, Vol 4, Iss 3, Pp 186-

2022 Volume 192

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, generating new variants that pose a threat to global health; therefore, it is imperative to obtain safe and broad-spectrum antivirals against SARS-CoV-2 and its variants. ... ...

Abstract	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, generating new variants that pose a threat to global health; therefore, it is imperative to obtain safe and broad-spectrum antivirals against SARS-CoV-2 and its variants. To this end, we screened compounds for their ability to inhibit viral entry, which is a critical step in virus infection. Twenty compounds that have been previously reported to inhibit SARS-CoV-2 replication were tested by using pseudoviruses containing the spike protein from the original strain (SARS-CoV-2-WH01). The cytotoxicity of these compounds was determined. Furthermore, we identified six compounds with strong antagonistic activity against the WH01 pseudovirus, and low cytotoxicity was identified. These compounds were then evaluated for their efficacy against pseudoviruses expressing the spike protein from B.1.617.2 (Delta) and B.1.1.529 (Omicron), the two most prevalent circulating strains. These assays demonstrated that two phenothiazine compounds, trifluoperazine 2HCl and thioridazine HCl, inhibit the infection of Delta and Omicron pseudoviruses. Finally, we discovered that these two compounds were highly effective against authentic SARS-CoV-2 viruses, including the WH01, Delta, and Omicron strains. Our study identified potential broad-spectrum SARS-CoV-2 inhibitors and provided insights into the development of novel therapeutics.
Keywords	SARS-CoV-2 ; Delta variant ; Omicron variant ; Trifluoperazine 2HCl ; Thioridazine HCl ; Antiviral therapy ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Acupuncture for Knee Osteoarthritis

Hao Tian / Liuyang Huang / Mingsheng Sun / Guixing Xu / Jiamei He / Zhuo Zhou / Fengyuan Huang / Yilin Liu / Fanrong Liang

BioMed Research International, Vol

A Systematic Review of Randomized Clinical Trials with Meta-Analyses and Trial Sequential Analyses

2022 Volume 2022

Abstract: Background. Knee osteoarthritis (KOA) can cause chronic pain and seriously affect the quality of patient lives. The continued emergence of high-quality RCTS requires us to update the quality of evidence. This study aims to evaluate the efficacy of ... ...

Abstract	Background. Knee osteoarthritis (KOA) can cause chronic pain and seriously affect the quality of patient lives. The continued emergence of high-quality RCTS requires us to update the quality of evidence. This study aims to evaluate the efficacy of acupuncture for KOA patients and calculate the required information size (RIS) to determine whether further clinical studies are required. Methods. We searched PubMed, Embase, WOS, CBM, CNKI, VIP, WHO ICTRP, ChiCTR, and Grey literature to collect randomized controlled trials (RCTs) of acupuncture for KOA from inception to December 2021. A meta-analysis was performed according to the Cochrane systematic review method by using Review Manager 5.4 and TSA 0.9.5.10 beta, and GRADE was used to evaluate the quality of the evidence. Trial sequential analysis was used to control random errors and calculate the required information size. Results. Eleven RCTs with 2484 patients were included in our meta-analysis, meeting the inclusion criteria for the meta-analysis. The meta-analysis indicated that acupuncture had beneficial effect on knee osteoarthritis in reducing pain [n=2387; SMD=−0.12, 95% CI (-0.20, -0.04); I2=0%] and improved patients function activities [n=2408; MD=−1.25, 95% CI (-1.97, -0.53); I2=0%], but true acupuncture showed no significant effect in relieve patient’s stiffness [n=1337; MD=−0.07, 95% CI (-0.30, 0.15); I2=0%]. We pooled the studies which found no significant difference in improving the quality life of mental [n=1462; SMD=0.02, 95% CI (-0.23, 0.27); I2=78%] and patients physical health (SF-36 or SF-12) [n=1745; SMD=1.01, 95% CI (-0.08, 2.11); I2=0%] compared with sham acupuncture. The pain and function TSA graphs indicated that cumulative Z-curves intersected with the traditional level of statistical favoring acupuncture, and more RCTs will required in the future studies. Conclusion. Acupuncture has beneficial effect on pain relief and improves function activities, and this treatment can be recommended as a beneficial alternative therapy in patients ...
Keywords	Medicine ; R
Subject code	610
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effectiveness and Safety of Electroacupuncture for Depression

Zhuo Zhou / Guixing Xu / Liuyang Huang / Hao Tian / Fengyuan Huang / Yilin Liu / Mingsheng Sun / Fanrong Liang

Evidence-Based Complementary and Alternative Medicine, Vol

A Systematic Review and Meta-Analysis

2022 Volume 2022

Abstract: Purpose. The purpose of this systematic review and meta-analysis was to comprehensively evaluate the efficacy and safety of electroacupuncture as an effective adjunctive therapy for patients with depression. Methods. Randomized controlled trials (RCTs) ... ...

Abstract	Purpose. The purpose of this systematic review and meta-analysis was to comprehensively evaluate the efficacy and safety of electroacupuncture as an effective adjunctive therapy for patients with depression. Methods. Randomized controlled trials (RCTs) about the treatment of depression by electroacupuncture therapy from inception to September 2021 were searched and collected in eight databases. HAMD, SDS, and Adverse Reactions were used as outcome indicators. The quality of relevant articles was evaluated using the Cochrane Collaboration’s risk of bias tool. The quality of evidence for each outcome was assessed through the Grading of Recommendations Assessment and the Development and Evaluation approach. Stata 15.0 software was used for data analysis. Results. A total of 16 depression-related RCTs were included in this meta-analysis. For the main outcome, electroacupuncture significantly reduced HAMD scores (I2:0.0%, SMD: −2.28% (95% CI−3.16 to −1.39)), and the quality of evidence was moderate. The improvement effect of electroacupuncture plus antidepressants was better than that of western drugs in patients with depression (I2:26.2%, SMD: −1.18% (95% CI−1.42 to −0.94)), and the quality of evidence was moderate. Electroacupuncture significantly reduced HAMD scores without significant heterogeneity (I2:0.0%, SMD: −3.76% (95%CI−5.78−1.73)). Studies with very low quality of evidence found that electroacupuncture was as effective as antidepressants in reducing SDS scores (I2:36.4%, WMD: −1.15% (95%CI−2.93–0.63)), and electroacupuncture was found to be more effective than sham electroacupuncture stimulation as well. Moderate quality evidence showed no statistical difference between electroacupuncture plus antidepressants/electroacupuncture and antidepressants (I2:0%, RR:1.05% (95%CI 0.73 to 1.53)). Conclusions. Our meta-analysis shows that electroacupuncture reduces HAMD scores. It is suggested to use electroacupuncture plus antidepressants to improve the curative effect and effectively reduce drug side effects.
Keywords	Other systems of medicine ; RZ201-999
Subject code	610
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Current research and development trends in floristic geography

Hang Sun / Tao Deng / Yongsheng Chen / Zhuo Zhou

Biodiversity Science, Vol 25, Iss 2, Pp 111-

2017 Volume 122

Abstract: This paper summarizes the research status, existing issues, and trends in floristic geography. There is now a wealth of research accumulation on floristic investigations, distribution types of genera, floristic regions, and regional floristic analysis. ... ...

Abstract	This paper summarizes the research status, existing issues, and trends in floristic geography. There is now a wealth of research accumulation on floristic investigations, distribution types of genera, floristic regions, and regional floristic analysis. It is also noted that most of these studies utilize simple statistical analyses, comparative studies, traditional methods, and single subjects, to provide a basic understanding and description of the floristic phenomenon, which is lacking spatial pattern formation processes and detailed exploration of formation mechanisms. Additionally, there are still some weak and non-existent areas of botanical investigation. Many existing specimens lack detailed or accurate information and the precise identification of plant species also needs to be much improved. At the same time, when analyzing the development trends of floristic geography, with the rapid development of related disciplines, including the development of biogeography and analysis methods and improvements, floristic geography research is an area of multidisciplinary integration, comprehensive research, and analysis. On the one hand, using database information, and combining ecology, paleobotany, and geology can allow us to probe into spatial pattern formation. On the other hand, combining phylogenetics, the tree of life, and molecular biogeography allow us to reveal floristic origins and evolution. The rapid development of various disciplines has given rise to a large amount of data, meanwhile, the emergence and application of new analytical methods and theories incorporate big data into floristic geography research, which will enhance qualitative understanding and description, and allow us to further explore the mechanisms of formation quantitatively.
Keywords	floristic geography ; floristic investigation ; comprehensive research ; ecology ; molecular phylogenetics ; biogeography ; big data ; Biology (General) ; QH301-705.5 ; Science ; Q
Subject code	910
Language	Chinese
Publishing date	2017-02-01T00:00:00Z
Publisher	Science Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Recent trends in acupuncture for chronic pain

Liuyang Huang / Guixing Xu / Mingsheng Sun / Chunyan Yang / Qin Luo / Hao Tian / Zhuo Zhou / Yilin Liu / Fengyuan Huang / Fanrong Liang / Ziwen Wang

Complementary Therapies in Medicine, Vol 72, Iss , Pp 102915- (2023)

A bibliometric analysis and review of the literature

2023

Abstract: Background: Acupuncture has been increasingly used in patients with chronic pain, yet no bibliometric analysis of acupuncture studies for chronic pain exists. Objectives: To investigate the characteristics, hotspots and frontiers of global scientific ... ...

Abstract	Background: Acupuncture has been increasingly used in patients with chronic pain, yet no bibliometric analysis of acupuncture studies for chronic pain exists. Objectives: To investigate the characteristics, hotspots and frontiers of global scientific output in acupuncture research for chronic pain over the past decade. Methods: We retrieved publications on acupuncture for chronic pain published from 2011 to 2022 from the Science Citation Index Expanded (SCI-expanded) of the Web of Science Core Collection (WoSCC). The co-occurrence relationships of journals/countries/institutions/authors/keywords were performed using VOSviewer V6.1.2, and CiteSpace V1.6.18 analyzed the clustering and burst analysis of keywords and co-cited references. Results: A total of 1616 articles were retrieved. The results showed that the number of annual publications on acupuncture for chronic pain has increased over time, with the main types of literature being original articles (1091 articles, 67.5 %) and review articles (351 articles, 21.7 %). China had the most publications (598 articles, 37 %), with Beijing University of Traditional Chinese Medicine (93 articles, 5.8 %) and Evidence-based Complementary and Alternative Medicine ranked first (169 articles, 10.45 %) as the most prolific affiliate and journal, respectively. Liang FR was the most productive author (43 articles), and the article published by Vickers Andrew J in 2012 had the highest number of citations (625 citations). Recently, ''acupuncture'' and ''pain'' appeared most frequently. The hot topics in acupuncture for chronic pain based on keywords clustering analysis were experimental design, hot diseases, interventions, and mechanism studies. According to burst analysis, the main research frontiers were functional connectivity (FC), depression, and risk. Conclusion: This study provides an in-depth perspective on acupuncture for chronic painstudies, revealing pivotal points, research hotspots, and research trends. Valuable ideas are provided for future research activities.
Keywords	Acupuncture ; Chronic pain ; Bibliometric analysis ; Review ; Co-citation analysis ; Other systems of medicine ; RZ201-999
Subject code	001
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Highly mutable tandem DNA repeats generate a cell wall protein variant more frequent in disease-causing Candida albicans isolates than in commensal isolates.

Zhuo Zhou / Zoe Jordens / Shuguang Zhang / Ningxin Zhang / Jan Schmid

PLoS ONE, Vol 12, Iss 6, p e

2017 Volume 0180246

Abstract: During adaptation to host environments, many microorganisms alter their cell surface. One mechanism for doing so is variation in the number of amino acid repeats in cell surface proteins encoded by hypermutable DNA tandem repeats. In the yeast Candida ... ...

Abstract	During adaptation to host environments, many microorganisms alter their cell surface. One mechanism for doing so is variation in the number of amino acid repeats in cell surface proteins encoded by hypermutable DNA tandem repeats. In the yeast Candida albicans, an opportunistic human pathogen, the gene SSR1 encodes a GPI-anchored cell wall protein with a structural role. It contains two regions consisting of tandem repeats, almost exclusively encoding the amino acid pair Ser-Ala. As expected, the repeat regions make SSR1 highly mutable. New SSR1 alleles arose with a frequency of 1.11×10-4 per cell division in serially propagated cells. We also observed a large number (25) of SSR1 alleles with different repeat lengths in a survey of 131 isolates from a global strain collection. C. albicans is diploid, and combinations of these allele generated 41 different SSR1 genotypes. In both repeat regions, nonsynonymous mutations were largely restricted to one particular repeat unit. Two very similar allele combinations were largely restricted to one clade, clade 1. Each combination was present in ~30% of 49 infection-causing clade 1 strains, but one was rare (2%), the other absent in 46 infection-causing strains representing the remainder of the species (P < 0.00018 and 0.00004; Fisher's exact test). These results indicate that both repeat regions are under selection and that amino acid repeat length polymorphisms generate Ssr1 protein variants most suitable for specific genetic backgrounds. One of these two allele combinations was 5.51 times more frequent, the other 1.75 times less frequent in 49 clade 1 strains that caused disease than in 36 commensal clade 1 strains (P = 0.0105; Chi2 test). This indicates that insertion and deletion of repeats not only generates clade-optimized Ssr1p variants, but may also assist in short-term adaptation when C. albicans makes the transition from commensal to pathogen.
Keywords	Medicine ; R ; Science ; Q
Subject code	572
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: PASTMUS

Xinyi Zhang / Di Yue / Yinan Wang / Yuexin Zhou / Ying Liu / Yeting Qiu / Feng Tian / Ying Yu / Zhuo Zhou / Wensheng Wei

Genome Biology, Vol 20, Iss 1, Pp 1-

mapping functional elements at single amino acid resolution in human cells

2019 Volume 16

Abstract: Abstract Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional ... ...

Abstract	Abstract Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DNA Sequences from CRISPR Tiling MUtagenesis Screening (PASTMUS), which provides a streamlined workflow and a bioinformatics pipeline to identify critical amino acids of proteins in their native biological contexts. Using this approach, we map six proteins—three bacterial toxin receptors and three cancer drug targets, and acquire their corresponding functional maps at amino acid resolution.
Keywords	Biology (General) ; QH301-705.5 ; Genetics ; QH426-470
Language	English
Publishing date	2019-12-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Steamed Zn/ZSM-5 catalysts for improved methanol aromatization with high stability

Wei, Zhenhao / Lifang Chen / Qingsheng Cao / Xuedong Zhu / Yarong Xu / Zhenhao Wen / Zhuo Zhou

Fuel processing technology. 2017 July, v. 162

2017

Abstract: The effects of steam treatment on Zn species modified ZSM-5 (Zn/ZSM-5) were systematically studied by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, 27Al MAS NMR spectroscopy, inductively coupled plasma-optical ... ...

Abstract	The effects of steam treatment on Zn species modified ZSM-5 (Zn/ZSM-5) were systematically studied by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, 27Al MAS NMR spectroscopy, inductively coupled plasma-optical emission spectroscopy, N2 physisorption, NH3 temperature-programmed desorption, infrared spectroscopy of adsorbed pyridine, Raman spectroscopy and thermogravimetric analysis. It was found that Zn species could efficiently enhance the hydrothermal stability of ZSM-5. Moreover, the ZnO species was enriched on the surface of ZSM-5 after steam treatment, while ZnOH+ species almost kept constant. The catalytic performance of steamed and non-steamed catalysts was evaluated in the process of methanol to aromatics (MTA). Zn/ZSM-5 steamed at 400°C exhibited higher selectivity for aromatics than non-steamed Zn/ZSM-5. The enhanced selectivity for aromatics was ascribed to the high retention ratio of Brønsted acid sites and newly created mesopores of Zn/ZSM-5 steamed at 400°C. In addition, the steamed catalysts showed lower deactivation rate and more coke on external surface compared to non-steamed catalysts, which could be the reason for superior catalyst stability.
Keywords	aluminum ; ammonia ; aromatic compounds ; catalysts ; catalytic activity ; desorption ; infrared spectroscopy ; methanol ; nuclear magnetic resonance spectroscopy ; pyridines ; Raman spectroscopy ; scanning electron microscopy ; stable isotopes ; steam ; thermogravimetry ; X-ray diffraction ; X-ray photoelectron spectroscopy ; zinc ; zinc oxide
Language	English
Dates of publication	2017-07
Size	p. 66-77.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1483666-x
ISSN	0378-3820
ISSN	0378-3820
DOI	10.1016/j.fuproc.2017.03.026
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis

Hongmin Zhang / Yuexin Zhou / Yinan Wang / Yige Zhao / Yeting Qiu / Xinyi Zhang / Di Yue / Zhuo Zhou / Wensheng Wei

Scientific Reports, Vol 8, Iss 1, Pp 1-

2018 Volume 9

Abstract: Abstract Engineered nucleases in genome editing manifest diverse efficiencies at different targeted loci. There is therefore a constant need to evaluate the mutation rates at given loci. T7 endonuclease 1 (T7E1) and Surveyor mismatch cleavage assays are ... ...

Abstract	Abstract Engineered nucleases in genome editing manifest diverse efficiencies at different targeted loci. There is therefore a constant need to evaluate the mutation rates at given loci. T7 endonuclease 1 (T7E1) and Surveyor mismatch cleavage assays are the most widely used methods, but they are labour and time consuming, especially when one must address multiple samples in parallel. Here, we report a surrogate system, called UDAR (Universal Donor As Reporter), to evaluate the efficiency of CRISPR/Cas9 in targeted mutagenesis. Based on the non-homologous end-joining (NHEJ)-mediated knock-in strategy, the UDAR-based assay allows us to rapidly evaluate the targeting efficiencies of sgRNAs. With one-step transfection and fluorescence-activated cell sorting (FACS) analysis, the UDAR assay can be completed on a large scale within three days. For detecting mutations generated by the CRISPR/Cas9 system, a significant positive correlation was observed between the results from the UDAR and T7E1 assays. Consistently, the UDAR assay could quantitatively assess bleomycin- or ICRF193-induced double-strand breaks (DSBs), which suggests that this novel strategy is broadly applicable to assessing the DSB-inducing capability of various agents. With the increasing impact of genome editing in biomedical studies, the UDAR method can significantly benefit the evaluation of targeted mutagenesis, especially for high-throughput purposes.
Keywords	Medicine ; R ; Science ; Q
Subject code	500
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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