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  1. Article ; Online: SFTSV-Nluc

    Haoran Sun / Zi-Wei Ye / Shuofeng Yuan

    EBioMedicine, Vol 100, Iss , Pp 104968- (2024)

    a useful tool for studying SFTSV biology and countermeasures

    2024  

    Keywords Medicine ; R ; Medicine (General) ; R5-920
    Language English
    Publishing date 2024-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: SARS-CoV-2 and COVID-19

    Kit-San Yuen / Zi-Wei Ye / Sin-Yee Fung / Pak-Hin Hinson Cheung / Chi-Ping Chan / Dong-Yan Jin

    Cell & Bioscience, Vol 11, Iss 1, Pp 1-

    revisiting the most important research questions

    2021  Volume 6

    Abstract: Abstract In February 2020, we highlighted the top nine important research questions on SARS-CoV-2 and COVID-19 concerning virus transmission, asymptomatic and presymptomatic virus shedding, diagnosis, treatment, vaccine development, origin of virus and ... ...

    Abstract Abstract In February 2020, we highlighted the top nine important research questions on SARS-CoV-2 and COVID-19 concerning virus transmission, asymptomatic and presymptomatic virus shedding, diagnosis, treatment, vaccine development, origin of virus and viral pathogenesis. These and related questions are revisited at the end of 2021 to shed light on the roadmap of bringing an end to the pandemic.
    Keywords SARS-CoV-2 ; COVID-19 ; Pandemic ; Endemic ; Vaccines ; Antivirals ; Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Overexpressing Arabidopsis thaliana ACBP6 in transgenic rapid-cycling Brassica napus confers cold tolerance

    Aruni Y. Alahakoon / Eden Tongson / Wei Meng / Zi-Wei Ye / Derek A. Russell / Mee-Len Chye / John F. Golz / Paul W. J. Taylor

    Plant Methods, Vol 18, Iss 1, Pp 1-

    2022  Volume 13

    Abstract: Abstract Background Rapid-cycling Brassica napus (B. napus-RC) has potential as a rapid trait testing system for canola (B. napus) because its life cycle is completed within 2 months while canola usually takes 4 months, and it is susceptible to the same ... ...

    Abstract Abstract Background Rapid-cycling Brassica napus (B. napus-RC) has potential as a rapid trait testing system for canola (B. napus) because its life cycle is completed within 2 months while canola usually takes 4 months, and it is susceptible to the same range of diseases and abiotic stress as canola. However, a rapid trait testing system for canola requires the development of an efficient transformation and tissue culture system for B. napus-RC. Furthermore, effectiveness of this system needs to be demonstrated by showing that a particular trait can be rapidly introduced into B. napus-RC plants. Results An in-vitro regeneration protocol was developed for B. napus-RC using 4-day-old cotyledons as the explant. High regeneration percentages, exceeding 70%, were achieved when 1-naphthaleneacetic acid (0.10 mg/L), 6-benzylaminopurine (1.0 mg/L), gibberellic acid (0.01 mg/L) and the ethylene antagonist silver nitrate (5 mg/L) were included in the regeneration medium. An average transformation efficiency of 16.4% was obtained using Agrobacterium-mediated transformation of B. napus-RC cotyledons using Agrobacterium strain GV3101 harbouring a plasmid with an NPTII (kanamycin-selectable) marker gene and the Arabidopsis thaliana cDNA encoding ACYL-COA-BINDING PROTEIN6 (AtACBP6). Transgenic B. napus-RC overexpressing AtACBP6 displayed better tolerance to freezing/frost than the wild type, with enhanced recovery from cellular membrane damage at both vegetative and flowering stages. AtACBP6-overexpressing B. napus-RC plants also exhibited lower electrolyte leakage and improved recovery following frost treatment, resulting in higher yields than the wild type. Ovules from transgenic AtACBP6 lines were better protected from frost than those of the wild type, while the developing embryos of frost-treated AtACBP6-overexpressing plants showed less freezing injury than the wild type. Conclusions This study demonstrates that B. napus-RC can be successfully regenerated and transformed from cotyledon explants and has the potential to ...
    Keywords Acyl-CoA-binding proteins ; Arabidopsis thaliana ACBP6 ; Brassica napus ; B. napus-RC cold tolerance ; Freezing tolerance ; Frost tolerance ; Plant culture ; SB1-1110 ; Biology (General) ; QH301-705.5
    Subject code 580
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: SARS-CoV-2 and COVID-19

    Kit-San Yuen / Zi -Wei Ye / Sin-Yee Fung / Chi-Ping Chan / Dong-Yan Jin

    Cell & Bioscience, Vol 10, Iss 1, Pp 1-

    The most important research questions

    2020  Volume 5

    Abstract: Abstract Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an ongoing global health emergency. Here we highlight nine most important research questions concerning virus transmission, ... ...

    Abstract Abstract Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an ongoing global health emergency. Here we highlight nine most important research questions concerning virus transmission, asymptomatic and presymptomatic virus shedding, diagnosis, treatment, vaccine development, origin of virus and viral pathogenesis.
    Keywords SARS-CoV-2 ; COVID-19 ; 2019 novel coronavirus (2019-nCoV) ; Novel coronavirus pneumonia (NCP) ; Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: The Arabidopsis Cytosolic Acyl-CoA-Binding Proteins Play Combinatory Roles in Pollen Development

    Hsiao, An-Shan / Edward C. Yeung / Mee-Len Chye / Zi-Wei Ye

    Plant & cell physiology. 2015 Feb., v. 56, no. 2

    2015  

    Abstract: In Arabidopsis, six acyl-CoA-binding proteins (ACBPs) have been identified and they have been demonstrated to function in plant stress responses and development. Three of these AtACBPs (AtACBP4–AtACBP6) are cytosolic proteins and all are expressed in ... ...

    Abstract In Arabidopsis, six acyl-CoA-binding proteins (ACBPs) have been identified and they have been demonstrated to function in plant stress responses and development. Three of these AtACBPs (AtACBP4–AtACBP6) are cytosolic proteins and all are expressed in floral organs as well as in other tissues. The roles of cytosolic AtACBPs in floral development were addressed in this study. To this end, a T-DNA insertional knockout mutant of acbp5 was characterized before use in crosses with the already available acbp4 and acbp6 T-DNA knockout mutants to examine their independent and combinatory functions in floral development. The single-gene knockout mutations did not cause any significant phenotypic changes, while phenotypic deficiencies affecting siliques and pollen were observed in the double mutants (acbp4acbp6 and acbp5acbp 6) and the acbp4acbp5acbp6 triple mutant. Vacuole accumulation in the acbp4acbp6 , acbp5acbp6 and acbp4acbp5acbp6 pollen was the most severe abnormality occurring in the double and triple mutants. Furthermore, scanning electron microscopy and transmission electron microscopy revealed exine and oil body defects in the acbp4acbp5acbp6 mutant, which also displayed reduced ability in in vitro pollen germination. Transgenic Arabidopsis expressing β-glucuronidase (GUS) driven from the various AtACBP promoters indicated that AtACBP6pro::GUS expression overlapped with AtACBP4pro::GUS expression in pollen grains and with AtACBP5pro::GUS expression in the microspores and tapetal cells. Taken together, these results suggest that the three cytosolic AtACBPs play combinatory roles in acyl-lipid metabolism during pollen development.
    Keywords Arabidopsis ; beta-glucuronidase ; crossing ; exine ; flowering ; flowers ; fruits ; knockout mutants ; lipid bodies ; metabolism ; microspores ; phenotype ; plant stress ; pollen ; pollen germination ; proteins ; scanning electron microscopy ; stress response ; tissues ; transfer DNA ; transgenic plants ; transmission electron microscopy ; vacuoles
    Language English
    Dates of publication 2015-02
    Size p. 322-333.
    Publishing place Japanese Society of Plant Physiologists.
    Document type Article
    ZDB-ID 208907-5
    ISSN 1471-9053 ; 0032-0781
    ISSN (online) 1471-9053
    ISSN 0032-0781
    DOI 10.1093/pcp/pcu163
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Broad-Spectrum Host-Based Antivirals Targeting the Interferon and Lipogenesis Pathways as Potential Treatment Options for the Pandemic Coronavirus Disease 2019 (COVID-19)

    Shuofeng Yuan / Chris Chun-Yiu Chan / Kenn Ka-Heng Chik / Jessica Oi-Ling Tsang / Ronghui Liang / Jianli Cao / Kaiming Tang / Jian-Piao Cai / Zi-Wei Ye / Feifei Yin / Kelvin Kai-Wang To / Hin Chu / Dong-Yan Jin / Ivan Fan-Ngai Hung / Kwok-Yung Yuen / Jasper Fuk-Woo Chan

    Viruses, Vol 12, Iss 628, p

    2020  Volume 628

    Abstract: The ongoing Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) signals an urgent need for an expansion in treatment options. In this study, we investigated the anti-SARS-CoV-2 activities of ...

    Abstract The ongoing Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) signals an urgent need for an expansion in treatment options. In this study, we investigated the anti-SARS-CoV-2 activities of 22 antiviral agents with known broad-spectrum antiviral activities against coronaviruses and/or other viruses. They were first evaluated in our primary screening in VeroE6 cells and then the most potent anti-SARS-CoV-2 antiviral agents were further evaluated using viral antigen expression, viral load reduction, and plaque reduction assays. In addition to remdesivir, lopinavir, and chloroquine, our primary screening additionally identified types I and II recombinant interferons, 25-hydroxycholesterol, and AM580 as the most potent anti-SARS-CoV-2 agents among the 22 antiviral agents. Betaferon (interferon-β1b) exhibited the most potent anti-SARS-CoV-2 activity in viral antigen expression, viral load reduction, and plaque reduction assays among the recombinant interferons. The lipogenesis modulators 25-hydroxycholesterol and AM580 exhibited EC 50 at low micromolar levels and selectivity indices of >10.0. Combinational use of these host-based antiviral agents with virus-based antivirals to target different processes of the SARS-CoV-2 replication cycle should be evaluated in animal models and/or clinical trials.
    Keywords coronavirus ; COVID-19 ; interferon ; AM580 ; 25-hydroxycholesterol ; treatment ; Microbiology ; QR1-502 ; covid19
    Subject code 570
    Language English
    Publishing date 2020-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Book ; Online: Broad-Spectrum Host-Based Antivirals Targeting the Interferon and Lipogenesis Pathways as Potential Treatment Options for the Pandemic Coronavirus Disease 2019 (COVID-19)

    Shuofeng Yuan / Chris Chun-Yiu Chan / Kenn Ka-Heng Chik / Jessica Oi-Ling Tsang / Ronghui Liang / Jianli Cao / Kaiming Tang / Jian-Piao Cai / Zi-Wei Ye / Feifei Yin / Kelvin Kai-Wang To / Hin Chu / Dong-Yan Jin / Ivan Fan-Ngai Hung / Kwok-Yung Yuen / Jasper Fuk-Woo Chan

    Viruses ; Volume 12 ; Issue 6

    2020  

    Abstract: The ongoing Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) signals an urgent need for an expansion in treatment options. In this study, we investigated the anti-SARS-CoV-2 activities of ...

    Abstract The ongoing Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) signals an urgent need for an expansion in treatment options. In this study, we investigated the anti-SARS-CoV-2 activities of 22 antiviral agents with known broad-spectrum antiviral activities against coronaviruses and/or other viruses. They were first evaluated in our primary screening in VeroE6 cells and then the most potent anti-SARS-CoV-2 antiviral agents were further evaluated using viral antigen expression, viral load reduction, and plaque reduction assays. In addition to remdesivir, lopinavir, and chloroquine, our primary screening additionally identified types I and II recombinant interferons, 25-hydroxycholesterol, and AM580 as the most potent anti-SARS-CoV-2 agents among the 22 antiviral agents. Betaferon (interferon-β

    1b) exhibited the most potent anti-SARS-CoV-2 activity in viral antigen expression, viral load reduction, and plaque reduction assays among the recombinant interferons. The lipogenesis modulators 25-hydroxycholesterol and AM580 exhibited EC50 at low micromolar levels and selectivity indices of >

    10.0. Combinational use of these host-based antiviral agents with virus-based antivirals to target different processes of the SARS-CoV-2 replication cycle should be evaluated in animal models and/or clinical trials.
    Keywords coronavirus ; COVID-19 ; interferon ; AM580 ; 25-hydroxycholesterol ; treatment ; covid19
    Subject code 570
    Language English
    Publishing date 2020-06-10
    Publisher Multidisciplinary Digital Publishing Institute
    Publishing country ch
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target

    Shuofeng Yuan / Hin Chu / Jasper Fuk-Woo Chan / Zi-Wei Ye / Lei Wen / Bingpeng Yan / Pok-Man Lai / Kah-Meng Tee / Jingjing Huang / Dongdong Chen / Cun Li / Xiaoyu Zhao / Dong Yang / Man Chun Chiu / Cyril Yip / Vincent Kwok-Man Poon / Chris Chung-Sing Chan / Kong-Hung Sze / Jie Zhou /
    Ivy Hau-Yee Chan / Kin-Hang Kok / Kelvin Kai-Wang To / Richard Yi-Tsun Kao / Johnson Yiu-Nam Lau / Dong-Yan Jin / Stanley Perlman / Kwok-Yung Yuen

    Nature Communications, Vol 10, Iss 1, Pp 1-

    2019  Volume 15

    Abstract: Viruses rely on host cell metabolism for replication, making these pathways potential therapeutic targets. Here, the authors show that AM580, a retinoid derivative and RAR-α agonist, affects replication of several RNA viruses by interfering with the ... ...

    Abstract Viruses rely on host cell metabolism for replication, making these pathways potential therapeutic targets. Here, the authors show that AM580, a retinoid derivative and RAR-α agonist, affects replication of several RNA viruses by interfering with the activity of SREBP.
    Keywords Science ; Q
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target

    Shuofeng Yuan / Hin Chu / Jasper Fuk-Woo Chan / Zi-Wei Ye / Lei Wen / Bingpeng Yan / Pok-Man Lai / Kah-Meng Tee / Jingjing Huang / Dongdong Chen / Cun Li / Xiaoyu Zhao / Dong Yang / Man Chun Chiu / Cyril Yip / Vincent Kwok-Man Poon / Chris Chung-Sing Chan / Kong-Hung Sze / Jie Zhou /
    Ivy Hau-Yee Chan / Kin-Hang Kok / Kelvin Kai-Wang To / Richard Yi-Tsun Kao / Johnson Yiu-Nam Lau / Dong-Yan Jin / Stanley Perlman / Kwok-Yung Yuen

    Nature Communications, Vol 10, Iss 1, Pp 1-

    2019  Volume 15

    Abstract: Viruses rely on host cell metabolism for replication, making these pathways potential therapeutic targets. Here, the authors show that AM580, a retinoid derivative and RAR-α agonist, affects replication of several RNA viruses by interfering with the ... ...

    Abstract Viruses rely on host cell metabolism for replication, making these pathways potential therapeutic targets. Here, the authors show that AM580, a retinoid derivative and RAR-α agonist, affects replication of several RNA viruses by interfering with the activity of SREBP.
    Keywords Science ; Q
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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