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  1. AU="Ziling Wang"
  2. AU="Sterne, Jonathan A. C."
  3. AU="Wang, Chiun-Chuan R."
  4. AU="Djomkam Zune, Alexandra Lindsey"
  5. AU="Magar, Naresh Khapangi"
  6. AU="Martínez-Gómez, David"
  7. AU="Prieto-Arevalo, Raquel"
  8. AU="McLaughlin, Noel P"
  9. AU=Wong Samson S Y AU=Wong Samson S Y
  10. AU="Campolo, Nicolás"
  11. AU="Abraham, Deborah"
  12. AU="Alqurashi, Thamer M A"
  13. AU="Stretton, Alexandra"
  14. AU="Teixeira, Jorge Juarez Vieira"
  15. AU="Keime, Céline"
  16. AU="Mateczun, Alfred"
  17. AU="Peterson, Erica"
  18. AU="Navarro-Amuedo, María Dolores"
  19. AU="Michellier, C"
  20. AU="Stephan Immenschuh"
  21. AU="Phillips, Sabrina"
  22. AU="Fadilah Fadilah"
  23. AU="Kira, Kei"
  24. AU="Modesto, Irene"
  25. AU="Bertha Rita Castillo Edua"
  26. AU="Hannah Danbury"
  27. AU="Elramly, Mohamed"
  28. AU="Louella, Michael"
  29. AU=Bolton Judy L
  30. AU="Riederer, Cordula"
  31. AU="Bao, Qi"
  32. AU="Tsang, Stephen H"
  33. AU="Isaranuwatchai, Wanrudee" AU="Isaranuwatchai, Wanrudee"
  34. AU="Goldring, Mary B"
  35. AU="Durrington, Charlotte"
  36. AU="Metka, M."
  37. AU="González Villarroel, Paula"
  38. AU="Gakuya, F."
  39. AU="Belloni-Fortina, Anna"
  40. AU="Teufel, Frank"

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  1. Artikel ; Online: Rebate Strategy Selection and Channel Coordination of Competing Two-Echelon Supply Chains

    Ziling Wang / Rong Zhang / Bin Liu

    Complexity, Vol

    2021  Band 2021

    Abstract: Rebate has long been a crucial tool that has attracted researchers from a diverse range of fields including marketing and supply chain management. When a manufacturer uses a retailer for reaching end customers, the rebate strategy undertakes an ... ...

    Abstract Rebate has long been a crucial tool that has attracted researchers from a diverse range of fields including marketing and supply chain management. When a manufacturer uses a retailer for reaching end customers, the rebate strategy undertakes an additional dimension. Here we show whether the two rebate strategies, manufacturer rebate and channel rebate, can be the optimal choice for the manufacturer and the retailer. And we aim at full coordination with rebate. Game theory is exploited to identify the equilibrium rebate decisions, which are fully characterized with two rebate strategies considering rebate sensitivity. Furthermore, we demonstrate how the decisions depend on parameters, such as market size, rebate redemption rate, and competition intensity in monopoly and duopoly supply chain systems. Our work also coordinates the supply chain with two coordination policies and examines if they can achieve full coordination. Counterintuitive findings suggest that the channel rebate with sensitivity and discrimination is not effective and the manufacturer rebate is the unique optimal option. Besides, the coordination can be realized with a centralized rebate in monopoly setting when the manufacturer forgoes her own interest. Then full coordination can be achieved in duopoly setting with a new coordination policy, rebate combination, given the redemption rate for the channel rebate is lower compared with the manufacturer rebate. Managerial insights are suggested that offering rebates with discrimination can have significant inventory and coordination policy implications and can lead to a double win under a well-controlled redemption rate.
    Schlagwörter Electronic computers. Computer science ; QA75.5-76.95
    Thema/Rubrik (Code) 330
    Sprache Englisch
    Erscheinungsdatum 2021-01-01T00:00:00Z
    Verlag Hindawi-Wiley
    Dokumenttyp Artikel ; Online
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  2. Artikel ; Online: Rg1 alleviates oxidative stress and spermatogonium apoptosis in D-gal-induced testicular toxicity by activating Akt

    Ziling Wang / Kunhang Du / Jiying Hou / Hanxianzhi Xiao / Ling Hu / Xiongbin Chen / Lu Wang / Yaping Wang

    Redox Report, Vol 28, Iss

    2023  Band 1

    Abstract: ABSTRACTObjectives: High reactive oxygen species (ROS) levels lead to cell death, and the testes are among the most vulnerable organs to oxidative damage. Rg1, an active ingredient extracted from the natural medicine ginseng, has potential anti- ... ...

    Abstract ABSTRACTObjectives: High reactive oxygen species (ROS) levels lead to cell death, and the testes are among the most vulnerable organs to oxidative damage. Rg1, an active ingredient extracted from the natural medicine ginseng, has potential anti-inflammatory, antioxidant and antiapoptotic properties. Our previous studies showed that Rg1 can effectively improve spermatogenic function in mice, but the specific mechanism remains unclear. The purpose of this study was to investigate the effect of Rg1 on oxidative stress and spermatogonium apoptosis in D-gal-induced testicular toxicity and elucidate the associated mechanism.Methods: Male C57BL/6 mice at 6–8 weeks of age were intraperitoneally injected with D-gal (200 mg/kg) for 42 days to establish a testicular injury model, and on day 16, 40 mg/kg Rg1-rich saline was injected intraperitoneally. Concurrently, we established an in vitro model of D-gal-damaged spermatogonia, which was treated with Rg1.Results: We found that treatment with the ginsenoside Rg1 reduced D-gal-induced oxidative stress and spermatogonium apoptosis in vivo and in vitro. Mechanistically, we found that Rg1 activated Akt/bad signaling and reduced D-gal-induced spermatogonium apoptosis.Discussion: We provide evidence showing that the antioxidant effect of Rg1 is mediated by the Akt/GSK-3β/NRF2 axis. Based on these findings, we consider Rg1 a potential treatment for testicular oxidative damage.
    Schlagwörter Ginsenoside Rg1 ; testis ; oxidative damage ; apoptosis ; Pathology ; RB1-214 ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 500
    Sprache Englisch
    Erscheinungsdatum 2023-12-01T00:00:00Z
    Verlag Taylor & Francis Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Rg1 Protects Hematopoietic Stem Cells from LiCl-Induced Oxidative Stress via Wnt Signaling Pathway

    Ziling Wang / Jieyu Xia / Jing Li / Linbo Chen / Xiongbin Chen / Yanyan Zhang / Lu Wang / Yaping Wang

    Evidence-Based Complementary and Alternative Medicine, Vol

    2022  Band 2022

    Abstract: Background. Ginsenoside Rg1 is a major component of ginseng with antioxidative and antiaging effects, which is a traditional Chinese medicine. In this study, we investigated the potential spillover and mechanism of action of Rg1 on LiCl-driven ... ...

    Abstract Background. Ginsenoside Rg1 is a major component of ginseng with antioxidative and antiaging effects, which is a traditional Chinese medicine. In this study, we investigated the potential spillover and mechanism of action of Rg1 on LiCl-driven hematopoietic stem cell aging. Results. Collect the purified Sca-1+ hematopoietic cells for differentiation ability detection and biochemical and molecular labeling. The experiment found that Rg1 plays an antiaging role in reversing the SA-β-gal staining associated with LiCl-induced hematopoietic stem cell senescence, the increase in p53 and p21 proteins, and sustained DNA damage. At the same time, Rg1 protects hematopoietic cells from the reduced differentiation ability caused by LiCl. In addition, Rg1 increased the excessive inhibition of intracellular GSK-3β protein, resulting in the maintenance of β-catenin protein levels in hematopoietic cells after LiCl treatment. Then, the target gene level of β-catenin can be maintained. Conclusions. Rg1 exerts the pharmacological effect of maintaining the activity of GSK-3β in Sca-1+ hematopoietic cells, enhances the antioxidant potential of cells, improves the redox homeostasis, and thus protects cells from the decline in differentiation ability caused by aging. This study provides a potential therapeutic strategy to reduce stem cell pool failure caused by chronic oxidative damage to hematopoietic stem cells.
    Schlagwörter Other systems of medicine ; RZ201-999
    Thema/Rubrik (Code) 571 ; 500
    Sprache Englisch
    Erscheinungsdatum 2022-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
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  4. Artikel ; Online: Aqueous Miscible Organic LDH Derived Ni-Based Catalysts for Efficient CO 2 Methanation

    Ziling Wang / Liang Huang / Tomas Ramirez Reina / Angelos M. Efstathiou / Qiang Wang

    Catalysts, Vol 10, Iss 1168, p

    2020  Band 1168

    Abstract: Converting CO 2 to methane via catalytic routes is an effective way to control the CO 2 content released in the atmosphere while producing value-added fuels and chemicals. In this study, the CO 2 methanation performance of highly dispersed Ni-based ... ...

    Abstract Converting CO 2 to methane via catalytic routes is an effective way to control the CO 2 content released in the atmosphere while producing value-added fuels and chemicals. In this study, the CO 2 methanation performance of highly dispersed Ni-based catalysts derived from aqueous miscible organic layered double hydroxides (AMO-LDHs) was investigated. The activity of the catalyst was found to be largely influenced by the chemical composition of Ni metal precursor and loading. A Ni-based catalyst derived from AMO-Ni 3 Al 1 -CO 3 LDH exhibited a maximum CO 2 conversion of 87.9% and 100% CH 4 selectivity ascribed to both the lamellar catalyst structure and the high Ni metal dispersion achieved. Moreover, due to the strong Ni metal–support interactions and abundant oxygen vacancy concentration developed, this catalyst also showed excellent resistance to carbon deposition and metal sintering. In particular, high stability was observed after 19 h in CO 2 /H 2 reaction at 360 °C.
    Schlagwörter AMO-LDH ; layered double oxides ; CO 2 hydrogenation ; methane ; Ni catalyst ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 290
    Sprache Englisch
    Erscheinungsdatum 2020-10-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
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  5. Artikel ; Online: A Shortcut Route to Close Nitrogen Cycle

    Shaoqu Xie / Chuhua Jia / Scott Sergio Go Ong / Ziling Wang / Mei-jun Zhu / Qiaojuan Wang / Yanhui Yang / Hongfei Lin

    iScience, Vol 23, Iss 5, Pp - (2020)

    Bio-Based Amines Production via Selective Deoxygenation of Chitin Monomers over Ru/C in Acidic Solutions

    2020  

    Abstract: Summary: Chitin, a long-chain polymer of N-acetyl-D-glucosamine (NAG) and the most abundant natural nitrogen-containing organic material in the world, is far under-utilized than other biomass resources. Herein, we demonstrate a highly efficient ... ...

    Abstract Summary: Chitin, a long-chain polymer of N-acetyl-D-glucosamine (NAG) and the most abundant natural nitrogen-containing organic material in the world, is far under-utilized than other biomass resources. Herein, we demonstrate a highly efficient deoxygenation process to convert chitin monomer, i.e., NAG, into various amines, which are the ubiquitous platform chemicals in chemical industry. In the presence of H2 and Ru/C catalyst, the oxygen atoms in the glucosamine molecules are removed in the form of H2O and/or CO/CO2, whereas CO is hydrogenated to CH4. By optimizing the reaction conditions, ∼50% yield of various amines was obtained via the selective deoxygenation of NAG. The reaction mechanism has been proposed. These findings not only promote shell biorefinery in green chemistry and fishery industry but also provide chemicals for material science, resulting in expanding cooperation in new areas such as clean energy, energy conservation, environment protection, and infrastructure.
    Schlagwörter Chemical Engineering ; Catalysis ; Green Chemistry ; Science ; Q
    Thema/Rubrik (Code) 540
    Sprache Englisch
    Erscheinungsdatum 2020-05-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
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  6. Artikel ; Online: Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell via GSK-3β and β-Catenin

    Ziling Wang / Rong Jiang / Lu Wang / Xiongbin Chen / Yue Xiang / Linbo Chen / Minghe Xiao / Li Ling / Yaping Wang

    Stem Cells International, Vol

    2020  Band 2020

    Abstract: Objectives. To demonstrate the effect of Ginsenoside Rg1 on the differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Subsequently, a rational mechanism for the detection of Rg1 which affects mesenchymal stem cell ... ...

    Abstract Objectives. To demonstrate the effect of Ginsenoside Rg1 on the differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Subsequently, a rational mechanism for the detection of Rg1 which affects mesenchymal stem cell differentiation was explored. Methods. Flow cytometry is used for cell identification. The differentiation ability of hBM-MSCs was studied by differentiation culture. SA-β-gal staining is used to detect cell senescence levels. Western blot and immunofluorescence were used to determine protein expression levels. RT-qPCR is used to detect mRNA expression levels. Results. Rg1 regulates the differentiation of hBM-MSCs. Differentiation culture analysis showed that Rg1 promoted cells to osteogenesis and chondrogenesis. Western blot results showed that Rg1 regulated the overactivation of the β-catenin signaling pathway and significantly adjusted the phosphorylation of GSK-3β. GSK-3β inhibitor (Licl) significantly increased Rg1-induced phosphorylation of GSK-3β, which in turn reduced Rg1-induced differentiation of hBM-MSCs. Conclusion. Ginsenoside Rg1 can reduce the excessive activation of the Wnt pathway in senescent cells by inhibiting the phosphorylation of GSK-3β and regulate the mesenchymal stem cell differentiation ability.
    Schlagwörter Internal medicine ; RC31-1245
    Thema/Rubrik (Code) 571
    Sprache Englisch
    Erscheinungsdatum 2020-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
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  7. Artikel: The regulation of ginsenoside Rg1 upon aging of bone marrow stromal cell contribute to delaying senescence of bone marrow mononuclear cells (BMNCs)

    Zeng, Yunlin / Lu Wang / Pengwei Jing / Wenxu Hu / Xiongbin Chen / Yaping Wang / Ziling Wang

    Life sciences. 2018 Sept. 15, v. 209

    2018  

    Abstract: To investigate the effect and mechanism of ginsenoside Rg1 antagonizing bone marrow stromal cells (BMSCs) aging, which contribute to the delaying senescence of hematopoietic cells in vitro and in vivo. Rg1 could reduce the effects of senility agent on ... ...

    Abstract To investigate the effect and mechanism of ginsenoside Rg1 antagonizing bone marrow stromal cells (BMSCs) aging, which contribute to the delaying senescence of hematopoietic cells in vitro and in vivo. Rg1 could reduce the effects of senility agent on BMSCs by decreasing the rate of SA-Gal positive cells, and increasing the proliferative ability of CCK8 cells. After BMNCs co-cultured with BMSCs which were treated by Rg1 in vitro, compared with BMNCs co-cultured with BMSCs from aging group, percentage of positive cell SA-Gal staining was decreased, the formation ability of CFU-Mix was enhanced, the proliferative ability was increased, and the apoptosis rate was decreased. In aging rat model, after treated with Rg1, the percentage of positive cell SA-Gal staining in BMSCs was significantly decreased, the proliferative ability was increased. After treated with Rg1, the percentage of positive cell SA-Gal staining in BMNCs was significantly decreased, the formation ability of CFU-Mix mixed colony was enhanced, ROS was decreased, and SOD activity was increased. Aging BMSCs could induce the senescence of BMNCs. Rg1 could antagonize the effect of d-gal on the aging of BMSCs both in vivo and in vitro, and restore the hematopoietic capacity of BMNCs through the different pathways.
    Schlagwörter animal models ; apoptosis ; bone marrow ; coculture ; hematopoietic stem cells ; staining ; stromal cells ; superoxide dismutase
    Sprache Englisch
    Erscheinungsverlauf 2018-0915
    Umfang p. 63-68.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel
    ZDB-ID 3378-9
    ISSN 1879-0631 ; 0024-3205
    ISSN (online) 1879-0631
    ISSN 0024-3205
    DOI 10.1016/j.lfs.2018.07.025
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  8. Artikel: Inhibitory Effect and Mechanism of Mesenchymal Stem Cells Cultured in 3D System on Hepatoma Cells HepG2

    Zhao, Diandian / Lingling Hou / Mengwu Pan / Jilei Hua / Ziling Wang / Jinsheng He / Honggang Hu

    Applied biochemistry and biotechnology. 2018 Jan., v. 184, no. 1

    2018  

    Abstract: Mesenchymal stem cells (MSCs) exhibit the feature of homing to tumor site and being immunosuppressive, which have broad prospects in tumor therapy. However, MSCs are commonly cultured in a two-dimensional (2D) condition, which would gradually loss some ... ...

    Abstract Mesenchymal stem cells (MSCs) exhibit the feature of homing to tumor site and being immunosuppressive, which have broad prospects in tumor therapy. However, MSCs are commonly cultured in a two-dimensional (2D) condition, which would gradually loss some in vivo important properties. In this study, we built a three-dimensional (3D) system with collagen/Matrigel scaffolds to culture MSCs. The results indicated that MSCs in 3D scaffolds showed higher proliferation ability than that of in 2D cells. In vitro, 3D-cultured MSC-conditioned media (CM) significantly inhibited the proliferation of hepatoma cells HepG2 than that of in 2D-cultured MSC-CM and control groups. In vivo, animal transplantation experiment showed that the treatment of 3D-cultured MSC-CM could further significantly delay the tumor initiation and decrease the tumor volume. The microarray, quantitative PCR, and ELISA assay found that MSCs cultured in the 3D system expressed and secreted more amounts of IL-24. RT-PCR and western blot results showed that IL-24 can activate JAK1-STAT3 pathway via IL22R1 and IL20R2, and further inhibit the proliferation of HepG2 cells. Taken together, these results demonstrated that MSCs cultured in the 3D system had an inhibitory effect on the proliferation of HepG2 cells, probably through secreting more IL-24, which activated JAK1-STAT3 signaling and finally inhibited the cell proliferation to delay tumor initiation. This study also provided a simpler and more reliable approach for MSCs to suppress tumor cells, and provided effective experimental data for clinical treatment of tumor and experimental basis.
    Schlagwörter Western blotting ; animals ; cell proliferation ; collagen ; enzyme-linked immunosorbent assay ; hepatoma ; human cell lines ; immunosuppression ; microarray technology ; neoplasm cells ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; stem cells ; therapeutics
    Sprache Englisch
    Erscheinungsverlauf 2018-01
    Umfang p. 212-227.
    Erscheinungsort Springer US
    Dokumenttyp Artikel
    ZDB-ID 392344-7
    ISSN 0273-2289
    ISSN 0273-2289
    DOI 10.1007/s12010-017-2533-1
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  9. Artikel ; Online: Human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation improves ovarian function in rats with premature ovarian insufficiency (POI) at least partly through a paracrine mechanism

    Li Ling / Xiushan Feng / Tianqin Wei / Yan Wang / Yaping Wang / Ziling Wang / Dongyuan Tang / Yanjing Luo / Zhengai Xiong

    Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-

    2019  Band 18

    Abstract: Abstract Background Chemotherapy can induce premature ovarian insufficiency (POI) and reduce fertility in young female patients. Currently, there is no effective therapy for POI. Human amnion-derived mesenchymal stem cells (hAD-MSCs) may be a promising ... ...

    Abstract Abstract Background Chemotherapy can induce premature ovarian insufficiency (POI) and reduce fertility in young female patients. Currently, there is no effective therapy for POI. Human amnion-derived mesenchymal stem cells (hAD-MSCs) may be a promising seed cell for regenerative medicine. This study investigated the effects and mechanisms of hAD-MSC transplantation on chemotherapy-induced POI in rats. Methods Chemotherapy-induced POI rat models were established by intraperitoneal injection of cyclophosphamide. Seventy-two female SD rats were randomly divided into control, POI, and hAD-MSC-treated groups. hAD-MSCs were labeled with PKH26 and injected into the tail veins of POI rats. To examine the underlying mechanisms, the differentiation of transplanted hAD-MSCs in the POI ovaries was analyzed by immunofluorescent staining. The in vitro expression of growth factors secreted by hAD-MSCs in hAD-MSC-conditioned media (hAD-MSC-CM) was analyzed by ELISA. Sixty female SD rats were divided into control, POI, and hAD-MSC-CM-treated groups, and hAD-MSC-CM was injected into the bilateral ovaries of POI rats. After hAD-MSC transplantation or hAD-MSC-CM injection, serum sex hormone levels, estrous cycles, ovarian pathological changes, follicle counts, granulosa cell (GC) apoptosis, and Bcl-2, Bax, and VEGF expression in ovaries were examined. Results PKH26-labeled hAD-MSCs mainly homed to ovaries after transplantation. hAD-MSC transplantation reduced ovarian injury and improved ovarian function in rats with POI. Transplanted hAD-MSCs were only located in the interstitium of ovaries, rather than in follicles, and did not express the typical markers of oocytes and GCs, which are ZP3 and FSHR, respectively. hAD-MSCs secreted FGF2, IGF-1, HGF, and VEGF, and those growth factors were detected in the hAD-MSC-CM. hAD-MSC-CM injection improved the local microenvironment of POI ovaries, leading to a decrease in Bax expression and an increase in Bcl-2 and endogenous VEGF expression in ovarian cells, which inhibited chemotherapy-induced GC apoptosis, promoted angiogenesis and regulated follicular development, thus partly reducing ovarian injury and improving ovarian function in rats with POI. Conclusions hAD-MSC transplantation can improve ovarian function in rats with chemotherapy-induced POI at least partly through a paracrine mechanism. The presence of a paracrine mechanism accounting for hAD-MSC-mediated recovery of ovarian function might be attributed to the growth factors secreted by hAD-MSCs.
    Schlagwörter Premature ovarian insufficiency (POI) ; Human amnion-derived mesenchymal stem cells (hAD-MSCs) ; Paracrine ; Conditioned media (CM) ; Growth factors ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2019-01-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
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  10. Artikel ; Online: Study on the Dynamic Biological Characteristics of Human Bone Marrow Mesenchymal Stem Cell Senescence

    Xiongbin Chen / Lu Wang / Jiyin Hou / Jin Li / Linbo Chen / Jieyu Xia / Ziling Wang / Minghe Xiao / Yaping Wang

    Stem Cells International, Vol

    2019  Band 2019

    Abstract: Objective. To preliminary explore the senescent dynamic changes of the bone marrow mesenchymal stem cells (BMMSCs) by human ageing and its possible mechanism. Methods. The bone marrows were harvested from healthy volunteers, and according to volunteers’ ... ...

    Abstract Objective. To preliminary explore the senescent dynamic changes of the bone marrow mesenchymal stem cells (BMMSCs) by human ageing and its possible mechanism. Methods. The bone marrows were harvested from healthy volunteers, and according to volunteers’ age, these were divided into group A (≤25 years), group B (26-45 years), group C (46-65 years), and group D (>65 years). Totally, the bone marrows were extracted from the posterior superior iliac spine from volunteers under aseptic conditions. Diluted with isovolumic PBS, followed by centrifugation at 1×105/cm2, cells were cultured in a 5% CO2 incubator at 37°C. After three passages, surface marker identification of hBMMSCs was tested by flow cytometry (FCM), oil red O staining was used to observe the ability of osteogenic differentiation, alkaline phosphatase (ALP) staining and the levels of osteocalcin (OST) in the supernatants were used to observe the ability of adipogenic differentiation, senescence-associated β-galactosidase (SA-β-Gal) staining was used to detect the senescent BMSCs, the ability of BMSC proliferation was detected by cell counting kit-8 (CCK-8), the distribution of the cell cycle was analyzed by flow cytometry (FCM), and malondialdehyde (MDA) content, total glutathione peroxidase, total antioxidant capacity, and total superoxide dismutase (SOD) activity was analyzed using enzymatic assay. Results. The BMSCs highly expressed CD73 and CD90, but lowly expressed CD34 and CD19/CD14. With age, osteogenic differentiation was markedly increased and audiogenic differentiation was significantly decreased. The number of SA-β-gal-positive cells was significantly increased, the proliferation ability of hBMMSCs declined, the BMSCs were held in the G1 phase, the MDA level of BMSCs was significantly increased, and total glutathione peroxidase, total antioxidant capacity, and SOD activity significantly declined. Conclusions. With age, the aging BMSCs were intensified; the mechanism may be related to oxidative damage mediated aging-related pathways.
    Schlagwörter Internal medicine ; RC31-1245
    Thema/Rubrik (Code) 571
    Sprache Englisch
    Erscheinungsdatum 2019-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
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