Article ; Online: Dynamics of antibody response and bacterial shedding of Mycoplasma hyorhinis and M. hyosynoviae in oral fluids from experimentally inoculated pigs.
2024 Volume 290, Page(s) 109999
Abstract: Mycoplasma hyorhinis (Mhr) and M. hyosynoviae (Mhs) are commensal organisms of the upper respiratory tract and tonsils but may also cause arthritis in pigs. In this study, 8-week-old cesarean-derived colostrum-deprived (CDCD) pigs (n = 30; 3 groups, 10 ... ...
| Abstract | Mycoplasma hyorhinis (Mhr) and M. hyosynoviae (Mhs) are commensal organisms of the upper respiratory tract and tonsils but may also cause arthritis in pigs. In this study, 8-week-old cesarean-derived colostrum-deprived (CDCD) pigs (n = 30; 3 groups, 10 pigs per group, 2 pigs per pen) were inoculated with Mhr, Mhs, or mock-inoculated with culture medium and then pen-based oral fluids were collected at different time points over the 56 days of the experimental study. Oral fluids tested by Mhr and Mhs quantitative real-time PCRs revealed Mhr DNA between day post inoculation (DPI) 5-52 and Mhs DNA between DPI 5-15. Oral fluids were likewise tested for antibody using isotype-specific (IgG, IgA, IgM) indirect ELISAs based on a recombinant chimeric polypeptide of variable lipoproteins (A-G) for Mhr and Tween 20-extracted surface proteins for Mhs. Mhr IgA was detected at DPI 7 and, relative to the control group, significant (p < 0.05) antibody responses were detected in the Mhr group between DPI 12-15 for IgM and DPI 36-56 for both IgA and IgG. In the Mhs group, IgM was detected at DPI 10 and significant (p < 0.05) IgG and IgA responses were detected at DPI 32-56 and DPI 44-56, respectively. This study demonstrated that oral fluid could serve as an effective and convenient antemortem sample for monitoring Mhr and Mhs in swine populations. |
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| MeSH term(s) | Swine ; Animals ; Mycoplasma hyorhinis/genetics ; Swine Diseases/microbiology ; Mycoplasma Infections/veterinary ; Mycoplasma Infections/microbiology ; Antibody Formation ; Bacterial Shedding ; Immunoglobulin M ; Immunoglobulin A ; DNA ; Immunoglobulin G |
| Chemical Substances | Immunoglobulin M ; Immunoglobulin A ; DNA (9007-49-2) ; Immunoglobulin G |
| Language | English |
| Publishing date | 2024-01-24 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 753154-0 |
| ISSN | 1873-2542 ; 0378-1135 |
| ISSN (online) | 1873-2542 |
| ISSN | 0378-1135 |
| DOI | 10.1016/j.vetmic.2024.109999 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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