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  1. Article ; Online: Evaluation of Vascular Endothelial Growth Factor Gene Expression in Recellularized Liver Tissue by Mouse Embryo Fibroblast.

    Homayoon Vala, Motahare / Bagheri, Hamed / Zinat, Sargazi / Bakhtiary, Negar / Pourbeiranvand, Shahram / Salehnia, Mojdeh

    Iranian biomedical journal

    2023  Volume 27, Issue 6, Page(s) 340–348

    Abstract: Background: The aim of the present study was to evaluate alterations in the vegf gene expression as an angiogenic factor in mouse embryo fibroblasts seeded on the decellularized liver fragments.: Methods: Liver tissue samples (n = 10) collected from ... ...

    Abstract Background: The aim of the present study was to evaluate alterations in the vegf gene expression as an angiogenic factor in mouse embryo fibroblasts seeded on the decellularized liver fragments.
    Methods: Liver tissue samples (n = 10) collected from adult male mice were randomly divided into decellularized and native control groups. Tissues were decellularized by treating with 1% Triton X-100 and 0.1% SDS for 24 hours and assessed by H&E staining and SEM. Then DNA content analysis and toxicity tests were performed. By centrifugation, DiI-labeled mouse embryo fibroblasts were seeded on each scaffold and cultured for one week. The recellularized scaffolds were studied by H&E staining, SEM, and LSCM. After RNA extraction and cDNA synthesis, the expression of the vegf gene in these samples was investigated using real-time RT-PCR.
    Results: Our observations showed that the decellularized tissues had morphology and porous structure similar to the control group, and their DNA content significantly reduced (p < 0.05) and reached to 4.12% of the control group. The MTT test indicated no significant cellular toxicity for the decellularized scaffolds. Light microscopy, SEM, and LSCM observations confirmed the attachment and penetration of embryonic fibroblast cells on the surface and into different depths of the scaffolds. There was no statistically significant difference in terms of vegf gene expression in the cultured cells in the presence and absence of a scaffold.
    Conclusion: The reconstructed scaffold had no effect on vegf gene expression. Decellularized mouse liver tissue recellularized by embryonic fibroblasts could have an application in regenerative medicine.
    MeSH term(s) Male ; Mice ; Animals ; Tissue Scaffolds/chemistry ; Vascular Endothelial Growth Factor A/genetics ; Liver ; DNA ; Gene Expression ; Tissue Engineering ; Extracellular Matrix
    Chemical Substances Vascular Endothelial Growth Factor A ; DNA (9007-49-2)
    Language English
    Publishing date 2023-11-01
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2489282-8
    ISSN 2008-823X ; 1028-852X
    ISSN (online) 2008-823X
    ISSN 1028-852X
    DOI 10.61186/ibj.3862
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E

    Fatemeh Rahimi Anbarkeh / Mohammad Reza Nikravesh / Mehdi Jalali / Hamid Reza Sadeghnia / Zinat Sargazi

    International Journal of Fertility and Sterility, Vol 13, Iss 2, Pp 154-

    2019  Volume 160

    Abstract: Background Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. ... ...

    Abstract Background Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. Materials and Methods In this experimental study, 30 male Wistar rats were divided into five groups (n=6 per group) consisting of control, sham (received olive oil), experimental group i (60 mg/kg DZN), experimental group ii (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group iii (200 mg/kg Vit E). After six weeks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL). Results Compared with the control group, DZN in the experimental group i decreased the number of PCNA-positive cells and increased the number of TUNEL-positive cells (P < 0.001). Vit E improved detrimental changes by the de- crease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (P < 0.001). Conclusion Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue.
    Keywords apoptosis ; diazinon ; proliferation ; testis ; vitamin e ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2019-07-01T00:00:00Z
    Publisher Royan Institute (ACECR), Tehran
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E

    Fatemeh Rahimi Anbarkeh / Mohammad Reza Nikravesh / Mehdi Jalali / Hamid Reza Sadeghnia / Zinat Sargazi

    International Journal of Fertility and Sterility, Vol 13, Iss 2, Pp 154-

    2019  Volume 160

    Abstract: Background Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. ... ...

    Abstract Background Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis. Materials and Methods In this experimental study, 30 male Wistar rats were divided into five groups (n=6 per group) consisting of control, sham (received olive oil), experimental group i (60 mg/kg DZN), experimental group ii (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group iii (200 mg/kg Vit E). After six weeks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL). Results Compared with the control group, DZN in the experimental group i decreased the number of PCNA-positive cells and increased the number of TUNEL-positive cells (P < 0.001). Vit E improved detrimental changes by the de- crease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (P < 0.001). Conclusion Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue.
    Keywords apoptosis ; diazinon ; proliferation ; testis ; vitamin e ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2019-07-01T00:00:00Z
    Publisher Royan Institute (ACECR), Tehran
    Document type Article ; Online
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  4. Article ; Online: Apoptotic Effect of Organophosphorus Insecticide Diazinon on Rat Ovary and Protective Effect of Vitamin E

    Zinat Sargazi / Mohammad Reza Nikravesh / Mehdi Jalali / Hamid Reza Sadeghnia / Fatemeh Rahimi Anbarkeh

    Iranian Journal of Toxicology, Vol 10, Iss 2, Pp 37-

    2016  Volume 44

    Abstract: Background: Diazinon (DZN) is an organophosphate insecticide widely utilized in agriculture all over the world and causes many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN ...

    Abstract Background: Diazinon (DZN) is an organophosphate insecticide widely utilized in agriculture all over the world and causes many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN on apoptosis of ovarian follicles in adult rats and to assess the protective role of vit E. Methods: Thirty adult female Wistar rats were divided into five groups: control group (without any intervention), sham group (received only pure olive oil, as solvent), experimental group 1 (DZN+olive oil, 60 mg/kg), experimental group 2 (vit E, 200 mg/kg), and experimental group 3 (DZN+vit E, the same dosage). All drugs were injected intraperitoneally, except vit E administrated by gavage. The animals were scarified after two weeks and left ovary was used to measure apoptosis of ovarian follicles. Results: The number apoptotic cells experimental group 1 increased significantly in contrast control group in secondary and graffian follicles (P< 0.001). Administration vit E plus DZN, significantly reduced apoptotic cells compared to DZN group (P< 0.001). Conclusion: DZN-induced apoptosis in secondary and graffian follicles and vit E inhibited apoptosis induced by DZN. Vit E might have a protective effect on DZN-induced ovarian toxicity.
    Keywords Apoptosis ; Diazinon ; Ovary ; Vitamin E. ; Toxicology. Poisons ; RA1190-1270 ; Public aspects of medicine ; RA1-1270 ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2016-02-01T00:00:00Z
    Publisher Arak University of Medical Sciences
    Document type Article ; Online
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  5. Article ; Online: Gender-Related Differences in Sensitivity to Diazinon in Gonads of Adult Rats and the Protective Effect of Vitamin E

    Zinat Sargazi / Mohammad Reza Nikravesh / Mehdi Jalali / Hamidreza Sadeghnia / Fatemeh Rahimi Anbarkeh / Leila Mohammadzadeh

    International Journal of Women's Health and Reproduction Sciences, Vol 3, Iss 1, Pp 40-

    2015  Volume 47

    Abstract: Objectives: Diazinon (DZN) is an organophosphate pesticide which is known to induce oxidative stress in the target tissues such as the reproductive system. The aim of present study was to compare and evaluate the effects of DZN on Malondialdehyde (MDA) ... ...

    Abstract Objectives: Diazinon (DZN) is an organophosphate pesticide which is known to induce oxidative stress in the target tissues such as the reproductive system. The aim of present study was to compare and evaluate the effects of DZN on Malondialdehyde (MDA) and Glutathione (GSH) levels in testis and ovary of rats and to assess the protective role of vitamin E. Materials and Methods: This study was conducted on 60 adult Wistar rats including 30 male and 30 female rats. Each of male and female groups included 30 rats and were divided into 5 groups: control (without any intervention), sham group (received only pure olive oil daily), treatment group 1 (received DZN+olive oil daily, 60 mg/kg), treatment group 2 (received DZN, 60 mg/kg + vitamin E, 200 mg/kg daily) and treatment group 3 (received vitamin E daily, 200 mg/kg). MDA and GSH levels were determined in gonads. Results: In both genders, due to administration of DZN, we found significant reduction in GSH content and increase in MDA level. The use of vitamin E plus DZN increased GSH content while decreased MDA level of ovary and testis compared with DZN treated rats. Gonad of female rats compared with male rats showed more increase in MDA level and further decline in GSH content in treatment groups 1 and 2. Conclusion: Oxidative stress contributes to DZN-induced sexual organ toxicity. Our results concluded that vitamin E may have a protective role in this toxicity. Female rats are likely more vulnerable to oxidative stress and its damages.
    Keywords Diazinon ; Gonads ; Oxidative Stress ; Vitamin E ; Gynecology and obstetrics ; RG1-991 ; Medicine ; R
    Subject code 590
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Aras Part Medical international Press
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Single dose effect of diazinon on biochemical parameters in testis tissue of adult rats and the protective effect of vitamin E

    Fatemeh Rahimi Anbarkeh / Mohammad Reza Nikravesh / Mehdi Jalali / Hamid Reza Sadeghnia / Zinat Sargazi / Leila Mohammdzadeh

    Iranian Journal of Reproductive Medicine, Vol 12, Iss 11, Pp 731-

    2014  Volume 736

    Abstract: Background: Diazinon (DZN) is an organophosphate pesticide that widely used for agricultural pest control all over the world. DZN affects target organs including reproductive system by inhibiting the activity of acetylcholinesterase and inducing ... ...

    Abstract Background: Diazinon (DZN) is an organophosphate pesticide that widely used for agricultural pest control all over the world. DZN affects target organs including reproductive system by inhibiting the activity of acetylcholinesterase and inducing oxidative stress. Vitamin E (α-tocopherol) is a strong antioxidant which inhibits free radicals, and probably can reduce lipid perxidation effectively in biological systems. Objective: The present study, aimed to evaluate the effects of DZN on malondialdehyde (MDA) and glutathione (GSH) levels in testis of rats and protective effect of vitamin E. Materials and Methods: In this experimental study, thirty adult male Wistar rats (200-250 gr) were divided into 5 groups (n= 6): control group (did not receive any material), sham group (received only pure olive oil), experimental group 1 (DZN, 60 mg/kg), experimental group 2 (Vit E, 200 mg/kg) and experimental group 3 (DZN+Vit E, with the same dose). All groups were sacrificed after 6 weeks and right testis was used to measure the MDA and GSH levels. The amount of MDA was determined by the thiobarbituric acid assay and 5, 5-Dithio-bis (2nitrobenzoic acid) DTNB-recycling protocol was used for GSH assay. Results: The results showed that DZN increased MDA level (p<0.001) and reduced GSH level (p<0.001). Administration of DZN plus vitamin E decreased the MDA level (p<0.001) and increased GSH level (p=0.001). Conclusion: DZN induced lipid peroxidation in the testis of rats. Vitamin E by its antioxidant activity was able to improve the toxic effect of DZN
    Keywords Gynecology and obstetrics ; RG1-991 ; Medicine ; R
    Subject code 571
    Language English
    Publishing date 2014-11-01T00:00:00Z
    Publisher Shahid Sadoghi University of Medical Sciences of Yazd
    Document type Article ; Online
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  7. Article ; Online: Diazinon-Induced Ovarian Toxicity and Protection by Vitamins E

    Zinat Sargazi / Mohammad Reza Nikravesh / Mehdi Jalali / Hamid Reza Sadeghnia / Fatemeh Rahimi Anbarkeh / Leila Mohammadzadeh

    Iranian Journal of Toxicology, Vol 8, Iss 26, Pp 1130-

    2014  Volume 1135

    Abstract: Background: DZN (diazinon) is an organophosphate insecticide that had been used in agriculture and for domestic and veterinary use for several years and caused many negative effects on plants and animal species, especially on human. The aim of present ... ...

    Abstract Background: DZN (diazinon) is an organophosphate insecticide that had been used in agriculture and for domestic and veterinary use for several years and caused many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN on MDA (malondialdehyde) and GSH (glutathione) levels in female rat reproductive tissue (ovary) and to assess the protective role of vitamin E. Methods: A total of 30 adult female Wistar rats were divided into five groups: control group (without any intervention), sham group (received only pure corn oil, as solvent), experimental group 1 (DZN+corn oil, 60 mg/kg), experimental group 2 (vitamin E, 200 mg/kg), and experimental group 3 (DZN+vitamin E, the same dosage). All drugs were injected intraperitoneally, except vitamin E which was administrated by gavage. The animals were scarified after two weeks and MDA as a marker of lipid peroxidation and GSH content were measured in ovarian tissue. Results: DZN reduced GSH content and increased MDA level in ovary compared with the control group (P<0.001). Vitamin E plus DZN increased GSH content but decreased DZN-induced MDA elevation in rat ovarian tissue. Conclusion: Oxidative stress contributes to DZN-induced ovarian toxicity. The results of this study suggested that vitamin E may have a protective effect on DZN-induced ovarian toxicity.
    Keywords Diazinon ; Glutathione ; Malondialdehyde ; Ovary ; Toxicology. Poisons ; RA1190-1270 ; Public aspects of medicine ; RA1-1270 ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2014-09-01T00:00:00Z
    Publisher Arak University of Medical Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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