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  1. Article ; Online: Single-cell patterning and characterisation of antibiotic persistent bacteria using bio-sCAPA.

    Boggon, Cameron / Mairpady Shambat, Srikanth / Zinkernagel, Annelies S / Secchi, Eleonora / Isa, Lucio

    Lab on a chip

    2023  Volume 23, Issue 23, Page(s) 5018–5028

    Abstract: In microbiology, accessing single-cell information within large populations is pivotal. Here we introduce bio-sCAPA, a technique for patterning bacterial cells in defined geometric arrangements and monitoring their growth in various nutrient environments. ...

    Abstract In microbiology, accessing single-cell information within large populations is pivotal. Here we introduce bio-sCAPA, a technique for patterning bacterial cells in defined geometric arrangements and monitoring their growth in various nutrient environments. We demonstrate bio-sCAPA with a study of subpopulations of antibiotic-tolerant bacteria, known as persister cells, which can survive exposure to high doses of antibiotics despite lacking any genetic resistance to the drug. Persister cells are associated with chronic and relapsing infections, yet are difficult to study due in part to a lack of scalable, single-cell characterisation methods. As >10
    MeSH term(s) Humans ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Staphylococcus aureus/genetics ; Bacteria ; Staphylococcal Infections/drug therapy ; Floxacillin
    Chemical Substances Anti-Bacterial Agents ; Floxacillin (43B2M34G2V)
    Language English
    Publishing date 2023-11-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d3lc00611e
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  2. Article ; Online: Antibacterial Neutrophil Effector Response: Ex Vivo Quantification of Regulated Cell Death Associated with Extracellular Trap Release.

    Schweizer, Tiziano A / Hertegonne, Sanne / Vulin, Clément / Zinkernagel, Annelies S / Mairpady Shambat, Srikanth

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2674, Page(s) 235–250

    Abstract: Regulated cell death (RCD) and the concomitant release of extracellular traps by neutrophils (NETs) constitute an important antibacterial effector response. Usually, the dynamic processes of RCD and NETs release are assessed independently of each other ... ...

    Abstract Regulated cell death (RCD) and the concomitant release of extracellular traps by neutrophils (NETs) constitute an important antibacterial effector response. Usually, the dynamic processes of RCD and NETs release are assessed independently of each other by either unspecific or time-consuming methods. Here, we describe a flow cytometry-based high-throughput analysis method incorporating neutrophil RCD and NETs release with visual live-imaging conformation upon ex vivo bacterial challenge. This combined approach allows to quantify and closely follow the kinetics of the dynamic neutrophil effector response towards bacterial infection.
    MeSH term(s) Neutrophils/metabolism ; Extracellular Traps/metabolism ; Regulated Cell Death ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism ; Flow Cytometry/methods
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2023-05-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3243-7_16
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  3. Article ; Online: Correction: Sepsis, a call for inclusion in the work plan of the European Center for Disease Prevention and Control.

    Giamarellos-Bourboulis, Evangelos J / Zinkernagel, Annelies S / De Robertis, Edoardo / Azoulay, Élie / De Luca, Daniele

    Intensive care medicine

    2023  Volume 49, Issue 10, Page(s) 1280

    Language English
    Publishing date 2023-08-24
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 80387-x
    ISSN 1432-1238 ; 0340-0964 ; 0342-4642 ; 0935-1701
    ISSN (online) 1432-1238
    ISSN 0340-0964 ; 0342-4642 ; 0935-1701
    DOI 10.1007/s00134-023-07197-3
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  4. Article ; Online: Sepsis, a call for inclusion in the work plan of the European Center for Disease Prevention and Control.

    Giamarellos-Bourboulis, Evangelos J / Zinkernagel, Annelies S / De Robertis, Edoardo / Azoulay, Élie / De Luca, Daniele

    Intensive care medicine

    2023  Volume 49, Issue 9, Page(s) 1138–1142

    MeSH term(s) Humans ; Sepsis/prevention & control
    Language English
    Publishing date 2023-08-01
    Publishing country United States
    Document type Letter
    ZDB-ID 80387-x
    ISSN 1432-1238 ; 0340-0964 ; 0342-4642 ; 0935-1701
    ISSN (online) 1432-1238
    ISSN 0340-0964 ; 0342-4642 ; 0935-1701
    DOI 10.1007/s00134-023-07127-3
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  5. Article: Identifying the Pathogen by Multiplex Polymerase Chain Reaction in Bone and Joint Infections: Challenges and Future.

    Achermann, Yvonne / Zinkernagel, Annelies S

    The Journal of rheumatology

    2018  Volume 45, Issue 11, Page(s) 1497–1500

    MeSH term(s) Arthritis, Infectious ; Humans ; Multiplex Polymerase Chain Reaction ; Synovial Fluid
    Language English
    Publishing date 2018-11-01
    Publishing country Canada
    Document type Editorial ; Comment
    ZDB-ID 194928-7
    ISSN 1499-2752 ; 0315-162X
    ISSN (online) 1499-2752
    ISSN 0315-162X
    DOI 10.3899/jrheum.180866
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    Zs.MO 135: Show issues

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  6. Article ; Online: MEndoB, a chimeric lysin featuring a novel domain architecture and superior activity for the treatment of staphylococcal infections.

    Roehrig, Christian / Huemer, Markus / Lorgé, Dominique / Arn, Fabienne / Heinrich, Nadine / Selvakumar, Lavanja / Gasser, Lynn / Hauswirth, Patrick / Chang, Chun-Chi / Schweizer, Tiziano A / Eichenseher, Fritz / Lehmann, Steffi / Zinkernagel, Annelies S / Schmelcher, Mathias

    mBio

    2024  Volume 15, Issue 2, Page(s) e0254023

    Abstract: Bacterial infections are a growing global healthcare concern, as an estimated annual 4.95 million deaths are associated with antimicrobial resistance (AMR). Methicillin- ... ...

    Abstract Bacterial infections are a growing global healthcare concern, as an estimated annual 4.95 million deaths are associated with antimicrobial resistance (AMR). Methicillin-resistant
    MeSH term(s) Humans ; Animals ; Mice ; Staphylococcus aureus ; Methicillin-Resistant Staphylococcus aureus ; Peptidoglycan ; Zebrafish ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/microbiology ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Staphylococcus ; N-Acetylmuramoyl-L-alanine Amidase/genetics ; N-Acetylmuramoyl-L-alanine Amidase/therapeutic use ; Sepsis/drug therapy
    Chemical Substances Peptidoglycan ; Anti-Bacterial Agents ; N-Acetylmuramoyl-L-alanine Amidase (EC 3.5.1.28)
    Language English
    Publishing date 2024-01-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.02540-23
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  7. Article ; Online: Improving antimicrobial treatment in terms of antimicrobial stewardship and health costs by an OPAT service.

    Burch, Andrea R / Ledergerber, Bruno / Ringer, Martin / Padrutt, Maria / Reiber, Claudine / Mayer, Fabienne / Zinkernagel, Annelies S / Eberhard, Nadia / Kaelin, Marisa B / Hasse, Barbara

    Infection

    2024  

    Abstract: Purpose: Outpatient parenteral antimicrobial therapy (OPAT) is a standard for antimicrobial therapy internationally. With this prospective cohort study, we aimed to assess the impact of an OPAT service as part of antimicrobial stewardship (AMS) and ... ...

    Abstract Purpose: Outpatient parenteral antimicrobial therapy (OPAT) is a standard for antimicrobial therapy internationally. With this prospective cohort study, we aimed to assess the impact of an OPAT service as part of antimicrobial stewardship (AMS) and evaluate the safety and efficiency of the program while illuminating the financial benefit for the hospital.
    Methods: Socio-demographic data, treatment regimen and outcomes were prospectively recorded for all patients assigned to the program of the OPAT unit of the University Hospital of Zurich between November 2018 and September 2022.
    Results: In total, we recorded 303 OPAT assignments of which 260 resulted in effective OPAT episodes. The 260 OPAT episodes were further optimized toward the choice of antimicrobial agent (n = 18) and length of therapy (n = 6). Moreover, OPAT resulted in alteration of patient assessment and care led by AMS strategies in 247 of 260 episodes (95%). While the bed days saved per year increased consistently with time, a total of 3934 in-hospital treatment days were saved amounting to a cost saving of 9,835,000 CHF over 47 months. Adverse events were recorded in 46 cases whilst only two of these have been the reason for readmission during OPAT treatment. Clinical cure was noted in 77% (199/260) and was negatively associated with Charlson Comorbidity Index (CCI; OR per 1 unit higher 0.85 (95% CI 0.78-0.93)).
    Conclusion: This study demonstrates the impact of an OPAT service in the framework of AMS as well as its benefits for the hospital whilst preserving safety and efficacy for the patient's parenteral antimicrobial treatment.
    Language English
    Publishing date 2024-02-29
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 185104-4
    ISSN 1439-0973 ; 0300-8126 ; 0173-2129
    ISSN (online) 1439-0973
    ISSN 0300-8126 ; 0173-2129
    DOI 10.1007/s15010-024-02194-0
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    Zs.A 881: Show issues Location:
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    Zs.MO 528: Show issues

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  8. Article ; Online: In Vitro Assessment of Bacterial Adhesion and Biofilm Formation on Novel Bioactive, Biodegradable Electrospun Fiber Meshes Intended to Support Tendon Rupture Repair.

    Miescher, Iris / Rieber, Julia / Schweizer, Tiziano A / Orlietti, Mariano / Tarnutzer, Andrea / Andreoni, Federica / Meier Buergisser, Gabriella / Giovanoli, Pietro / Calcagni, Maurizio / Snedeker, Jess G / Zinkernagel, Annelies S / Buschmann, Johanna

    ACS applied materials & interfaces

    2024  Volume 16, Issue 5, Page(s) 6348–6355

    Abstract: The surgical repair of a ruptured tendon faces two major problems: specifically increased fibrous adhesion to the surrounding tissue and inferior mechanical properties of the scar tissue compared to the native tissue. Bacterial attachment to implant ... ...

    Abstract The surgical repair of a ruptured tendon faces two major problems: specifically increased fibrous adhesion to the surrounding tissue and inferior mechanical properties of the scar tissue compared to the native tissue. Bacterial attachment to implant materials is an additional problem as it might lead to severe infections and impaired recovery. To counteract adhesion formation, two novel implant materials were fabricated by electrospinning, namely, a random fiber mesh containing hyaluronic acid (HA) and poly(ethylene oxide) (PEO) in a ratio of 1:1 (HA/PEO 1:1) and 1:4 (HA/PEO 1:4), respectively. Electrospun DegraPol (DP) treated with silver nanoparticles (DP-Ag) was developed to counteract the bacterial attachment. The three novel materials were compared to the previously described DP and DP with incorporated insulin-like growth factor-1 (DP-IGF-1), two implant materials that were also designed to improve tendon repair. To test whether the materials are prone to bacterial adhesion and biofilm formation, we assessed 10 strains of
    MeSH term(s) Humans ; Bacterial Adhesion ; Silver/pharmacology ; Silver/chemistry ; Insulin-Like Growth Factor I/pharmacology ; Metal Nanoparticles/chemistry ; Tendon Injuries/surgery ; Anti-Bacterial Agents/pharmacology ; Biofilms ; Tendons
    Chemical Substances Silver (3M4G523W1G) ; Insulin-Like Growth Factor I (67763-96-6) ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-01-30
    Publishing country United States
    Document type Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.3c15710
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  9. Article ; Online: Antibiotic resistance and persistence-Implications for human health and treatment perspectives.

    Huemer, Markus / Mairpady Shambat, Srikanth / Brugger, Silvio D / Zinkernagel, Annelies S

    EMBO reports

    2020  Volume 21, Issue 12, Page(s) e51034

    Abstract: Antimicrobial resistance (AMR) and persistence are associated with an elevated risk of treatment failure and relapsing infections. They are thus important drivers of increased morbidity and mortality rates resulting in growing healthcare costs. ... ...

    Abstract Antimicrobial resistance (AMR) and persistence are associated with an elevated risk of treatment failure and relapsing infections. They are thus important drivers of increased morbidity and mortality rates resulting in growing healthcare costs. Antibiotic resistance is readily identifiable with standard microbiological assays, and the threat imposed by antibiotic resistance has been well recognized. Measures aiming to reduce resistance development and spreading of resistant bacteria are being enforced. However, the phenomenon of bacteria surviving antibiotic exposure despite being fully susceptible, so-called antibiotic persistence, is still largely underestimated. In contrast to antibiotic resistance, antibiotic persistence is difficult to measure and therefore often missed, potentially leading to treatment failures. In this review, we focus on bacterial mechanisms allowing evasion of antibiotic killing and discuss their implications on human health. We describe the relationship between antibiotic persistence and bacterial heterogeneity and discuss recent studies that link bacterial persistence and tolerance with the evolution of antibiotic resistance. Finally, we review persister detection methods, novel strategies aiming at eradicating bacterial persisters and the latest advances in the development of new antibiotics.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacteria/genetics ; Drug Resistance, Bacterial ; Drug Resistance, Microbial/genetics ; Humans
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2020-12-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2020896-0
    ISSN 1469-3178 ; 1469-221X
    ISSN (online) 1469-3178
    ISSN 1469-221X
    DOI 10.15252/embr.202051034
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    Zs.MG 41: Show issues

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  10. Article ; Online: Reply to Perez and Patel.

    Leimer, Nadja / Zinkernagel, Annelies S

    The Journal of infectious diseases

    2016  Volume 215, Issue 3, Page(s) 490–491

    MeSH term(s) Hydrogen-Ion Concentration ; Lysosomes
    Language English
    Publishing date 2016-12-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiw504
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