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  1. Book ; Online: Chapter Effect of Local Delivery of GDNF Conjugated Iron Oxide Nanoparticles on Nerve Regeneration along Long Chitosan Nerve Guide

    Michael, Tolmasov / Almog, Mara / Ziv-Polat, Ofra / Mandelbaum-Livnat, Mira M. / Geuna, Stefano / Bitan, Yifat / Morano, Michela / Biran, Tali / Rochkind, Shimon / Fregnan, Federica / Reider, Evgeniy / Shahar, Abraham / Nissan, Moshe

    2017  

    Keywords Neurosciences ; GDNF conjugated iron oxide nanoparticles, chitosan tube, peripheral nerve regeneration
    Size 1 Online-Ressource
    Publisher InTechOpen
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021047208
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book ; Online: Chapter Peripheral Nerve Reconstruction Using Enriched Chitosan Conduits

    Ronchi, Giulia / Shahar, Abraham / Rochkind, Shimon / Reider, Evgeniy / Bitan, Yifat / Geuna, Stefano / Viano, Nicoletta / Koren, Akiva / Mandelbaum-Livnat, Mira M. / Morano, Michela / Ziv-Polat, Ofra / Biron, Tali

    2017  

    Keywords Biomedical engineering ; peripheral nerve injury, chitosan guidance conduit, conduit acetylation, hyaluronic acid gel, magnetic fibrin hydrogel
    Size 1 Online-Ressource
    Publisher InTechOpen
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021047544
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  3. Article: Application of iron oxide anoparticles in neuronal tissue engineering.

    Ziv-Polat, Ofra / Margel, Shlomo / Shahar, Abraham

    Neural regeneration research

    2015  Volume 10, Issue 2, Page(s) 189–191

    Language English
    Publishing date 2015-04-01
    Publishing country India
    Document type Journal Article
    ZDB-ID 2388460-5
    ISSN 1876-7958 ; 1673-5374
    ISSN (online) 1876-7958
    ISSN 1673-5374
    DOI 10.4103/1673-5374.152364
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

    Ziv-Polat, Ofra / Skaat, Hadas / Shahar, Abraham / Margel, Shlomo

    International journal of nanomedicine

    2012  Volume 7, Page(s) 1259–1274

    Abstract: Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the ... ...

    Abstract Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.
    MeSH term(s) Animals ; Cell Differentiation/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Fibrin/chemistry ; Fibrin/metabolism ; Fibrinogen/chemistry ; Fibrinogen/metabolism ; Fibroblast Growth Factors/chemistry ; Fibroblast Growth Factors/pharmacology ; Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry ; Magnetite Nanoparticles/chemistry ; Microscopy, Fluorescence ; Nasal Mucosa/cytology ; Rats ; Thrombin/chemistry ; Thrombin/metabolism ; Tissue Engineering/instrumentation ; Tissue Scaffolds/chemistry
    Chemical Substances Magnetite Nanoparticles ; Hydrogel, Polyethylene Glycol Dimethacrylate (25852-47-5) ; Fibroblast Growth Factors (62031-54-3) ; Fibrin (9001-31-4) ; Fibrinogen (9001-32-5) ; Thrombin (EC 3.4.21.5)
    Language English
    Publishing date 2012-03-06
    Publishing country New Zealand
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2364941-0
    ISSN 1178-2013 ; 1176-9114
    ISSN (online) 1178-2013
    ISSN 1176-9114
    DOI 10.2147/IJN.S26533
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6606: Show issues Location:
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  5. Article ; Online: Enhancement of the growth and differentiation of nasal olfactory mucosa cells by the conjugation of growth factors to functional nanoparticles.

    Skaat, Hadas / Ziv-Polat, Ofra / Shahar, Abraham / Margel, Shlomo

    Bioconjugate chemistry

    2011  Volume 22, Issue 12, Page(s) 2600–2610

    Abstract: Growth factors are critical components in the tissue engineering approach. Basic fibroblast growth factor (bFGF), a representative growth factor, stimulates the cellular functions of various cells and has been used extensively for the repair and ... ...

    Abstract Growth factors are critical components in the tissue engineering approach. Basic fibroblast growth factor (bFGF), a representative growth factor, stimulates the cellular functions of various cells and has been used extensively for the repair and regeneration of tissues. The in vivo half-life time of free bFGF is short, about 3-10 min, due to rapid enzymatic degradation. Stabilization of the bFGF was accomplished by the covalent or physical conjugation of this factor to fluorescent maghemite (γ-Fe(2)O(3)) nanoparticles. In the present study, nasal olfactory mucosa (NOM) cells from adult rats were cultured in suspension on chitosan microcarriers (MCs) in the presence of the nonconjugated or bFGF-conjugated nanoparticles, or the free factor. The floating cells/nonconjugated, conjugated, or free bFGF/MCs aggregates were then seeded in a viscous gel. In this manuscript, we are the first to report that the stabilization of the factor by its conjugation to these nanoparticles significantly improved NOM cell-proliferation properties (migration, growth, and differentiation), compared to the same concentration, or even five times higher, of the free factor. This novel approach may significantly contribute to the advancement of the tissue engineering field.
    MeSH term(s) Animals ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Ferric Compounds/chemistry ; Fibroblast Growth Factor 2/administration & dosage ; Fibroblast Growth Factor 2/chemistry ; Nanoparticles/chemistry ; Olfactory Mucosa/cytology ; Rats ; Tissue Engineering
    Chemical Substances Ferric Compounds ; Fibroblast Growth Factor 2 (103107-01-3) ; ferric oxide (1K09F3G675)
    Language English
    Publishing date 2011-12-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1024041-x
    ISSN 1520-4812 ; 1043-1802
    ISSN (online) 1520-4812
    ISSN 1043-1802
    DOI 10.1021/bc200454k
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3400: Show issues Location:
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  6. Article: Functionalised, photostable, fluorescent polystyrene nanoparticles of narrow size-distribution

    Pellach, Michal / Goldshtein, Jenny / Ziv-Polat, Ofra / Margel, Shlomo

    Journal of Photochemistry & Photobiology, A: Chemistry. 2012 Jan. 15, v. 228, no. 1

    2012  

    Abstract: Fluorescent nanoparticles continue to be of wide interest, as they have many advantages over single fluorescent molecules for biological imaging and sensing applications, such as increased fluorescence intensity and reduced photobleaching. In the ... ...

    Abstract Fluorescent nanoparticles continue to be of wide interest, as they have many advantages over single fluorescent molecules for biological imaging and sensing applications, such as increased fluorescence intensity and reduced photobleaching. In the following work, styrene was copolymerised with a newly synthesised, fluorescein-based, vinylic crosslinking monomer, by emulsion polymerisation, to create a series of different sized fluorescent nanoparticles (35–100nm), each of narrow size-distribution. The particles were found to be highly fluorescent and with lower photobleaching compared to fluorescein isothiocyanate (FITC), offering an attractive alternative. The fluorescence excitation and emission spectra were recorded, being similar to fluorescein, but with interesting variation in the excitation spectra. The particles also have a wide range of potential uses, such as examining particle uptake activity of a macrophage cell line, also demonstrated. The nanoparticles were coated with albumin to provide functionality for potential conjugation to biological targeting agents.
    Keywords albumins ; crosslinking ; emulsions ; fluorescein ; fluorescence ; image analysis ; macrophages ; nanoparticles ; photobleaching ; polymerization ; polystyrenes ; styrene
    Language English
    Dates of publication 2012-0115
    Size p. 60-67.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1491828-6
    ISSN 1010-6030
    ISSN 1010-6030
    DOI 10.1016/j.jphotochem.2011.11.012
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Enhancement of incisional wound healing by thrombin conjugated iron oxide nanoparticles.

    Ziv-Polat, Ofra / Topaz, Moris / Brosh, Tamar / Margel, Shlomo

    Biomaterials

    2010  Volume 31, Issue 4, Page(s) 741–747

    Abstract: Thrombin has been clinically used for topical hemostasis and wound management for more than six decades. The half-life of thrombin in human plasma is shorter than 15s due to close control by inhibitors. In order to stabilize the thrombin, it was bound to ...

    Abstract Thrombin has been clinically used for topical hemostasis and wound management for more than six decades. The half-life of thrombin in human plasma is shorter than 15s due to close control by inhibitors. In order to stabilize the thrombin, it was bound to maghemite (gamma-Fe(2)O(3)) nanoparticles, as demonstrated in previous work. The aim of the present study was to examine the efficiency of the bound thrombin for wound healing applications compared to the free thrombin. For this purpose incisional wounds on rat skin were treated with a mixture of fibrinogen, CaCl(2) solution and free or bound thrombin. The wounds' edges were then approximated by skin staples. The control incisional wounds were closed with staples only. In the course of 28 days of healing the highest values of skin tensile strength were observed following treatment with the bound thrombin. Significantly lower values of tensile strength were observed following treatment with the free thrombin, and the lowest values were obtained following treatment with staples only. The histological findings correlate with the mechanical strength measurements, which demonstrate the most advanced stages of healing following treatment with the bound thrombin.
    MeSH term(s) Animals ; Calcium Chloride/pharmacology ; Calcium Chloride/therapeutic use ; Dermatologic Surgical Procedures ; Ferric Compounds/chemistry ; Ferric Compounds/pharmacology ; Ferric Compounds/therapeutic use ; Fibrinogen/pharmacology ; Fibrinogen/therapeutic use ; Humans ; Male ; Nanoparticles/chemistry ; Nanoparticles/therapeutic use ; Rats ; Rats, Wistar ; Skin/pathology ; Tensile Strength ; Thrombin/chemistry ; Thrombin/pharmacology ; Thrombin/therapeutic use ; Wound Healing/drug effects
    Chemical Substances Ferric Compounds ; ferric oxide (1K09F3G675) ; Fibrinogen (9001-32-5) ; Thrombin (EC 3.4.21.5) ; Calcium Chloride (M4I0D6VV5M)
    Language English
    Publishing date 2010-02
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 603079-8
    ISSN 1878-5905 ; 0142-9612
    ISSN (online) 1878-5905
    ISSN 0142-9612
    DOI 10.1016/j.biomaterials.2009.09.093
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2371: Show issues Location:
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  8. Article: Magnetic scaffolds enriched with bioactive nanoparticles for tissue engineering.

    Skaat, Hadas / Ziv-Polat, Ofra / Shahar, Abraham / Last, David / Mardor, Yael / Margel, Shlomo

    Advanced healthcare materials

    2012  Volume 1, Issue 2, Page(s) 168–171

    Abstract: Novel magnetic fibrin hydrogel scaffolds for cell implantation and tissue engineering are reported. The magnetic scaffolds are produced by the interaction between thrombin-conjugated maghemite nanoparticles of narrow size distribution and fibrinogen. ... ...

    Abstract Novel magnetic fibrin hydrogel scaffolds for cell implantation and tissue engineering are reported. The magnetic scaffolds are produced by the interaction between thrombin-conjugated maghemite nanoparticles of narrow size distribution and fibrinogen. These scaffolds, enriched with growth factor conjugated fluorescent maghemite nanoparticles, provide a supporting 3D environment for massive proliferation of various cell types, and can be successfully visualized by MRI.
    MeSH term(s) Animals ; Cell Aggregation/physiology ; Ferric Compounds/chemistry ; Fibrin/chemistry ; Fibrin/metabolism ; Fibrin/ultrastructure ; Magnetics ; Materials Testing ; Nanoparticles/chemistry ; Nanoparticles/ultrastructure ; Rats ; Rats, Inbred Lew ; Tissue Engineering/instrumentation
    Chemical Substances Ferric Compounds ; ferric oxide (1K09F3G675) ; Fibrin (9001-31-4)
    Language English
    Publishing date 2012-03
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649576-4
    ISSN 2192-2659 ; 2192-2640
    ISSN (online) 2192-2659
    ISSN 2192-2640
    DOI 10.1002/adhm.201100056
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6966: Show issues Location:
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  9. Article ; Online: Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials.

    Morano, Michela / Wrobel, Sandra / Fregnan, Federica / Ziv-Polat, Ofra / Shahar, Abraham / Ratzka, Andreas / Grothe, Claudia / Geuna, Stefano / Haastert-Talini, Kirsten

    International journal of nanomedicine

    2014  Volume 9, Page(s) 5289–5306

    Abstract: Purpose: Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by ... ...

    Abstract Purpose: Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by neurotrophic factor delivery within the nerve conduits via nanotechnology or stem cell engineering and transplantation.
    Methods: Here, we comparatively investigated the bioactivity of selected neurotrophic factors conjugated to iron oxide nanoparticles (np-NTFs) and of bone marrow-derived stem cells genetically engineered to overexpress those neurotrophic factors (NTF-BMSCs). The neurite outgrowth inductive activity was monitored in culture systems of adult and neonatal rat sensory dorsal root ganglion neurons as well as in the cell line from rat pheochromocytoma (PC-12) cell sympathetic culture model system.
    Results: We demonstrate that np-NTFs reliably support numeric neurite outgrowth in all utilized culture models. In some aspects, especially with regard to their long-term bioactivity, np-NTFs are even superior to free NTFs. Engineered NTF-BMSCs proved to be less effective in induction of sensory neurite outgrowth but demonstrated an increased bioactivity in the PC-12 cell culture system. In contrast, primary nontransfected BMSCs were as effective as np-NTFs in sensory neurite induction and demonstrated an impairment of neuronal differentiation in the PC-12 cell system.
    Conclusion: Our results evidence that nanotechnology as used in our setup is superior over stem cell engineering when it comes to in vitro models for PNR. Furthermore, np-NTFs can easily be suspended in regenerative hydrogel matrix and could be delivered that way to nerve conduits for future in vivo studies and medical application.
    MeSH term(s) Animals ; Cell Engineering/methods ; Magnetite Nanoparticles/chemistry ; Mesenchymal Stromal Cells/cytology ; Nanotechnology/methods ; Nerve Growth Factors/genetics ; Nerve Growth Factors/metabolism ; Nerve Regeneration ; Neurites/physiology ; PC12 Cells ; Rats ; Rats, Wistar ; Regenerative Medicine
    Chemical Substances Magnetite Nanoparticles ; Nerve Growth Factors
    Language English
    Publishing date 2014
    Publishing country New Zealand
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2364941-0
    ISSN 1178-2013 ; 1176-9114
    ISSN (online) 1178-2013
    ISSN 1176-9114
    DOI 10.2147/IJN.S71951
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Synthesis, fluorescence and biodistribution of a bone-targeted near-infrared conjugate.

    Mizrahi, Dana M / Ziv-Polat, Ofra / Perlstein, Benny / Gluz, Eran / Margel, Shlomo

    European journal of medicinal chemistry

    2011  Volume 46, Issue 10, Page(s) 5175–5183

    Abstract: Enhanced imaging of early-stage bone abnormalities, such as primary tumors or metastases is highly required as the widely-used bone scan frequently lacks the desired sensitivity. Near IR (NIR) fluorescence imaging affords high contrast and enhanced ... ...

    Abstract Enhanced imaging of early-stage bone abnormalities, such as primary tumors or metastases is highly required as the widely-used bone scan frequently lacks the desired sensitivity. Near IR (NIR) fluorescence imaging affords high contrast and enhanced sensitivity, as body tissue expresses minimal autofluorescence at NIR range (600-1200 nm). Indocyanine green (ICG), a biocompatible NIR dye, is widely used in the imaging of various organs, such as liver, heart and blood circulation. We report the preparation and in-vivo testing of a bone-targeting ICG derivative, in comparison to the parent molecule(s). Since ICG itself is chemically unreactive, and could not form conjugates, we prepared two novel ICG conjugatable derivatives. The overall ICG structure was maintained while only a replacement of one or two sulfonate groups with carboxylic acids resulted in new linkers for covalent binding to biomolecules. These derivatives were evaluated for their fluorescence and biodistribution in comparison to ICG and were found to be comparable. One of the novel ICG-derivatives was conjugated to a bone-targeting moiety and this new compound was found to bind to growing regions of the skeleton, and emit fluorescence for as long as two weeks in young mice.
    MeSH term(s) Animals ; Bone and Bones/metabolism ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/pharmacokinetics ; Indocyanine Green/analogs & derivatives ; Indocyanine Green/pharmacokinetics ; Male ; Mice ; Mice, Inbred BALB C ; Spectrometry, Fluorescence/methods ; Spectroscopy, Near-Infrared/methods ; Tissue Distribution
    Chemical Substances Fluorescent Dyes ; Indocyanine Green (IX6J1063HV)
    Language English
    Publishing date 2011-10
    Publishing country France
    Document type Journal Article
    ZDB-ID 188597-2
    ISSN 1768-3254 ; 0009-4374 ; 0223-5234
    ISSN (online) 1768-3254
    ISSN 0009-4374 ; 0223-5234
    DOI 10.1016/j.ejmech.2011.08.040
    Database MEDical Literature Analysis and Retrieval System OnLINE

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