Article ; Online: Antiviral activity of triptolide on herpes simplex virus in vitro.
Immunity, inflammation and disease
2022 Volume 10, Issue 7, Page(s) e667
Abstract: Background: Herpes simplex virus-type 1 (HSV-1) can cause diseases, especially amongst neonates and immunocompromised hosts. Hence, developing a novel anti-HSV-1 drug with low-level toxicity is vital. Triptolide (TP), a diterpenoid triepoxide is a ... ...
Abstract | Background: Herpes simplex virus-type 1 (HSV-1) can cause diseases, especially amongst neonates and immunocompromised hosts. Hence, developing a novel anti-HSV-1 drug with low-level toxicity is vital. Triptolide (TP), a diterpenoid triepoxide is a natural product with range of bioactivity qualities. Methods: In this study, viral infection was assessed in different phases of the HSV-1 replication cycle on A549 cells, using various assays, such as adsorption inhibition assay, penetration inhibition assay, time-of-addition assay, and quantitative polymerase chain reaction (qPCR). Results: The results indicate that TP can effectively inhibit HSV-1 infection in the lowest range of concentration. TP exhibited significant inhibitory effect on HSV-1 plaque formation, with 50% effective concentration (EC50) of 0.05 µM. Furthermore, the time-of-addition assay suggests that TP has viral inhibitory effects when it was added less than 8 h postinfection (h.p.i.). This result is further confirmed by decline in the expression viral immediate-early genes (ICP4, ICP22, and ICP27) in 6 h.p.i in the TP-treated group compared to the control group, evaluated by real-time qPCR. The Western blotting result was also consistent with the previous findings, which confirms that TP can positively affect ICP4 during HSV-1 infection. Conclusions: The TP also showed antiviral activity against HSV-1. This dose-dependent activity is an indication of a particular cellular component, rather than cytotoxicity that has mediated its function. Finally, the result suggest a new approach for an effective treatment option of the HSV-1 infections. |
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MeSH term(s) | Animals ; Antiviral Agents/pharmacology ; Chlorocebus aethiops ; Diterpenes/metabolism ; Diterpenes/pharmacology ; Epoxy Compounds ; Herpes Simplex/drug therapy ; Herpesvirus 1, Human ; Humans ; Infant, Newborn ; Phenanthrenes ; Vero Cells |
Chemical Substances | Antiviral Agents ; Diterpenes ; Epoxy Compounds ; Phenanthrenes ; triptolide (19ALD1S53J) |
Language | English |
Publishing date | 2022-06-27 |
Publishing country | England |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2740382-8 |
ISSN | 2050-4527 ; 2050-4527 |
ISSN (online) | 2050-4527 |
ISSN | 2050-4527 |
DOI | 10.1002/iid3.667 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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