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  1. Article ; Online: Detection of a High-Dose Hook Effect and Evaluation of Dilutions of Urine Myoglobin Specimens Using a Serum Myoglobin Assay.

    Hunsaker, Joshua Joon Hyung / La'ulu, Sonia Leilani / Zupan, Emily / Patel, Dhwani / Pandya, Vrajesh / Rudolf, Joseph William

    Annals of laboratory medicine

    2024  Volume 44, Issue 4, Page(s) 367–370

    MeSH term(s) Humans ; Myoglobin/analysis ; Urinalysis
    Chemical Substances Myoglobin
    Language English
    Publishing date 2024-03-14
    Publishing country Korea (South)
    Document type Letter
    ZDB-ID 2677441-0
    ISSN 2234-3814 ; 2234-3814
    ISSN (online) 2234-3814
    ISSN 2234-3814
    DOI 10.3343/alm.2023.0427
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Pretreatment of Body Fluid Specimens Using Hyaluronidase and Ultracentrifugation.

    La'ulu, Sonia L / Turner, Devon R / Zupan, Emily / Genzen, Jonathan R

    Laboratory medicine

    2021  Volume 52, Issue 5, Page(s) 469–476

    Abstract: Objective: Viscous body fluids present challenges during clinical laboratory testing. The present study was conducted to evaluate the effectiveness of hyaluronidase (HYAL) and ultracentrifugation (UC) pretreatment for a variety of body fluids before ... ...

    Abstract Objective: Viscous body fluids present challenges during clinical laboratory testing. The present study was conducted to evaluate the effectiveness of hyaluronidase (HYAL) and ultracentrifugation (UC) pretreatment for a variety of body fluids before clinical chemistry testing.
    Methods: The following body fluids were evaluated: biliary/hepatic, cerebrospinal, dialysate, drain, pancreatic, pericardial, peritoneal/ascites, pleural, synovial, and vitreous. Analytes assessed included amylase, total bilirubin, cancer antigen 19-9, carcinoembryonic antigen, cholesterol, chloride, creatinine, glucose, lactate dehydrogenase, lipase, potassium, rheumatoid factor, sodium, total protein, triglycerides, urea nitrogen, and uric acid.
    Results: Observed percentage differences between HYAL treated and untreated fluids were less than ±15% for all analytes investigated, with a small number showing statistical significance (P <.05). In addition, UC showed increased variability for limited body fluid/analyte combinations.
    Conclusion: The HYAL treatment effectively reduced viscosity for body fluids. Validation of specimen pretreatment processes ensures acceptable analytical performance and the absence of unanticipated interferences.
    MeSH term(s) Body Fluids ; Humans ; Hyaluronoglucosaminidase ; Laboratories, Clinical ; Sodium ; Ultracentrifugation
    Chemical Substances Sodium (9NEZ333N27) ; Hyaluronoglucosaminidase (EC 3.2.1.35)
    Language English
    Publishing date 2021-02-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 391758-7
    ISSN 1943-7730 ; 0007-5027
    ISSN (online) 1943-7730
    ISSN 0007-5027
    DOI 10.1093/labmed/lmaa115
    Database MEDical Literature Analysis and Retrieval System OnLINE

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