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Article: Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses' Outbreak in Rio de Janeiro, Brazil.

de Souza, Thiara Manuele Alves / de Lima, Raquel Curtinhas / Solórzano, Victor Edgar Fiestas / Damasco, Paulo Vieira / de Souza, Luiz José / Sanchez-Arcila, Juan Camilo / Guimarães, Gabriel Macedo Costa / Paiva, Iury Amâncio / da Rocha Queiroz Lima, Monique / de Bruycker-Nogueira, Fernanda / Tomé, Larissa Cristina Teixeira / Coelho, Mariana Rosa Inácio / da Silva, Sandro Patroca / de Oliveira-Pinto, Luzia Maria / de Azeredo, Elzinandes Leal / Dos Santos, Flavia Barreto

Pathogens (Basel, Switzerland)

2022 Volume 11, Issue 2

Abstract: The co-circulation of chikungunya virus (CHIKV), dengue virus (DENV) and Zika virus (ZIKV) in Rio de Janeiro (RJ), Brazil, caused a challenging triple epidemic, as they share similar clinical signs and symptoms and geographical distribution. Here, we ... ...

Abstract	The co-circulation of chikungunya virus (CHIKV), dengue virus (DENV) and Zika virus (ZIKV) in Rio de Janeiro (RJ), Brazil, caused a challenging triple epidemic, as they share similar clinical signs and symptoms and geographical distribution. Here, we aimed to investigate the clinical and laboratorial aspects of chikungunya suspected cases assisted in RJ during the 2018 outbreak, focusing on the differential diagnosis with dengue and zika. All suspected cases were submitted to molecular and/or serological differential diagnostic approaches to arboviruses. A total of 242 cases suspected of arbovirus infection were investigated and 73.6% (178/242) were molecular and/or serologically confirmed as chikungunya. In RT-qPCR confirmed cases, cycle threshold (Ct) values ranged from 15.46 to 35.13, with acute cases presenting lower values. Chikungunya cases were mainly in females (64%) and the most frequently affected age group was adults between 46 to 59 years old (27%). Polyarthralgia affected 89% of patients, especially in hands and feet. No dengue virus (DENV) and Zika virus (ZIKV) infections were confirmed by molecular diagnosis, but 9.5% (23/242) had serological evidence of DENV exposure by the detection of specific anti-DENV IgM or NS1, and 42.7% (76/178) of chikungunya positive cases also presented recent DENV exposure reflected by a positive anti-DENV IgM or NS1 result. A significantly higher frequency of arthritis (
Language	English
Publishing date	2022-02-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens11020245
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Article: Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil

Pathogens. 2022 Feb. 14, v. 11, no. 2

2022

Abstract	The co-circulation of chikungunya virus (CHIKV), dengue virus (DENV) and Zika virus (ZIKV) in Rio de Janeiro (RJ), Brazil, caused a challenging triple epidemic, as they share similar clinical signs and symptoms and geographical distribution. Here, we aimed to investigate the clinical and laboratorial aspects of chikungunya suspected cases assisted in RJ during the 2018 outbreak, focusing on the differential diagnosis with dengue and zika. All suspected cases were submitted to molecular and/or serological differential diagnostic approaches to arboviruses. A total of 242 cases suspected of arbovirus infection were investigated and 73.6% (178/242) were molecular and/or serologically confirmed as chikungunya. In RT-qPCR confirmed cases, cycle threshold (Ct) values ranged from 15.46 to 35.13, with acute cases presenting lower values. Chikungunya cases were mainly in females (64%) and the most frequently affected age group was adults between 46 to 59 years old (27%). Polyarthralgia affected 89% of patients, especially in hands and feet. No dengue virus (DENV) and Zika virus (ZIKV) infections were confirmed by molecular diagnosis, but 9.5% (23/242) had serological evidence of DENV exposure by the detection of specific anti-DENV IgM or NS1, and 42.7% (76/178) of chikungunya positive cases also presented recent DENV exposure reflected by a positive anti-DENV IgM or NS1 result. A significantly higher frequency of arthritis (p = 0.023) and limb edema (p < 0.001) was found on patients with CHIKV monoinfection compared to dengue patients and patients exposed to both viruses. Lastly, phylogenetic analysis showed that the chikungunya cases were caused by the ECSA genotype. Despite the triple arboviruses’ epidemic in the state of RJ, most patients with fever and arthralgia investigated here were diagnosed as chikungunya cases, and the incidence of CHIKV/DENV co-detection was higher than that reported in other studies.
Keywords	Chikungunya virus ; Dengue virus ; Zika virus ; arboviruses ; arthritis ; dengue ; edema ; fever ; genotype ; geographical distribution ; phylogeny ; Brazil
Language	English
Dates of publication	2022-0214
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens11020245
Database	NAL-Catalogue (AGRICOLA)

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Article: Dengue virus tetra-epitope peptide expressed in lettuce chloroplasts for potential use in dengue diagnosis

Maldaner, Franciele Roberta / Aragão, Francisco José Lima / dos Santos, Flávia Barreto / Franco, Octavio Luiz / da Rocha Queiroz Lima, Monique / de Oliveira Resende, Renato / Vasques, Raquel Medeiros / Nagata, Tatsuya

Applied microbiology and biotechnology. 2013 July, v. 97, no. 13

2013

Abstract: Dengue virus causes about 100 million cases of dengue disease per year in the world. Laboratory diagnosis is done mainly by serological techniques, which in many cases use crude virus extracts that may cause cross-reactions to other flaviviruses. These ... ...

Abstract	Dengue virus causes about 100 million cases of dengue disease per year in the world. Laboratory diagnosis is done mainly by serological techniques, which in many cases use crude virus extracts that may cause cross-reactions to other flaviviruses. These undesirable cross-reactions can be reduced or eliminated by using recombinant proteins based on restricted epitopes. Aiming to decrease flaviviral cross-reactions and non-specific interactions in dengue serological assays, a plant expression system was chosen for recombinant antigen production as a reliable and inexpensive dengue diagnostic tool. In the present report, the lettuce plastid transformation system was applied to achieve efficient and stable tetra-epitope peptide antigen production, and its reactivity was evaluated. For this purpose, one putative epitope at positions 34 to 57 of E protein within the junction site of domains I and II of dengue virus (DENV) 1 to 4 serotypes linked by glycine linkers was expressed in lettuce chloroplasts. The potential immunoreactivity for the four DENV serotypes was evaluated using sera from patients of positive and negative dengue cases. Results indicated an overall sensitivity of 71.7� % and specificity of 100� %. No cross-reactions with the sera of yellow fever-positive or healthy individuals vaccinated against yellow fever were observed. This novel approach may provide an alternative system for the large-scale production of dengue recombinant antigens useful for serodiagnosis.
Keywords	Dengue virus ; chloroplasts ; cross reaction ; dengue ; epitopes ; fever ; lettuce ; patients ; recombinant antigens ; recombinant proteins ; serotypes ; viruses
Language	English
Dates of publication	2013-07
Size	p. 5721-5729.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-013-4918-6
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Twenty years of DENV-2 activity in Brazil: molecular characterization and phylogeny of strains isolated from 1990 to 2010.

Faria, Nieli Rodrigues da Costa / Nogueira, Rita Maria Ribeiro / de Filippis, Ana Maria Bispo / Simões, Jaqueline Bastos Santos / Nogueira, Fernanda de Bruycker / da Rocha Queiroz Lima, Monique / dos Santos, Flavia Barreto

PLoS neglected tropical diseases

2013 Volume 7, Issue 3, Page(s) e2095

Abstract: In Brazil, dengue has been a major public health problem since its introduction in the 1980s. Phylogenetic studies constitute a valuable tool to monitor the introduction and spread of viruses as well as to predict the potential epidemiological ... ...

Abstract	In Brazil, dengue has been a major public health problem since its introduction in the 1980s. Phylogenetic studies constitute a valuable tool to monitor the introduction and spread of viruses as well as to predict the potential epidemiological consequences of such events. Aiming to perform the molecular characterization and phylogenetic analysis of DENV-2 during twenty years of viral activity in the country, viral strains isolated from patients presenting different disease manifestations (n = 34), representing six states of the country, from 1990 to 2010, were sequenced. Partial genome sequencing (genes C/prM/M/E) was performed in 25 DENV-2 strains and full-length genome sequencing (coding region) was performed in 9 strains. The percentage of similarity among the DENV-2 strains in this study and reference strains available in Genbank identified two groups epidemiologically distinct: one represented by strains isolated from 1990 to 2003 and one from strains isolated from 2007 to 2010. No consistent differences were observed on the E gene from strains isolated from cases with different clinical manifestations analyzed, suggesting that if the disease severity has a genetic origin, it is not only due to the differences observed on the E gene. The results obtained by the DENV-2 full-length genome sequencing did not point out consistent differences related to a more severe disease either. The analysis based on the partial and/or complete genome sequencing has characterized the Brazilian DENV-2 strains as belonging to the Southeast Asian genotype, however a distinction of two Lineages within this genotype has been identified. It was established that strains circulating prior DENV-2 emergence (1990-2003) belong to Southeast Asian genotype, Lineage I and strains isolated after DENV-2 emergence in 2007 belong to Southeast Asian genotype, Lineage II. Furthermore, all DENV-2 strains analyzed presented an asparagine (N) in E390, previously identified as a probable genetic marker of virulence observed in DHF strains from Asian origin. The percentage of identity of the latter with the Dominican Republic strain isolated in 2001 combined to the percentage of divergence with the strains first introduced in the country in the 1990s suggests that those viruses did not evolve locally but were due to a new viral Lineage introduction in the country from the Caribbean.
MeSH term(s)	Brazil/epidemiology ; Cluster Analysis ; Dengue/epidemiology ; Dengue/virology ; Dengue Virus/classification ; Dengue Virus/genetics ; Dengue Virus/isolation & purification ; Genotype ; Humans ; Molecular Epidemiology ; Molecular Sequence Data ; Phylogeny ; RNA, Viral/genetics ; Sequence Analysis, DNA
Chemical Substances	RNA, Viral
Language	English
Publishing date	2013-03-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2429704-5
ISSN	1935-2735 ; 1935-2727
ISSN (online)	1935-2735
ISSN	1935-2727
DOI	10.1371/journal.pntd.0002095
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Article ; Online: Dengue virus tetra-epitope peptide expressed in lettuce chloroplasts for potential use in dengue diagnosis.

Applied microbiology and biotechnology

2013 Volume 97, Issue 13, Page(s) 5721–5729

Abstract	Dengue virus causes about 100 million cases of dengue disease per year in the world. Laboratory diagnosis is done mainly by serological techniques, which in many cases use crude virus extracts that may cause cross-reactions to other flaviviruses. These undesirable cross-reactions can be reduced or eliminated by using recombinant proteins based on restricted epitopes. Aiming to decrease flaviviral cross-reactions and non-specific interactions in dengue serological assays, a plant expression system was chosen for recombinant antigen production as a reliable and inexpensive dengue diagnostic tool. In the present report, the lettuce plastid transformation system was applied to achieve efficient and stable tetra-epitope peptide antigen production, and its reactivity was evaluated. For this purpose, one putative epitope at positions 34 to 57 of E protein within the junction site of domains I and II of dengue virus (DENV) 1 to 4 serotypes linked by glycine linkers was expressed in lettuce chloroplasts. The potential immunoreactivity for the four DENV serotypes was evaluated using sera from patients of positive and negative dengue cases. Results indicated an overall sensitivity of 71.7% and specificity of 100%. No cross-reactions with the sera of yellow fever-positive or healthy individuals vaccinated against yellow fever were observed. This novel approach may provide an alternative system for the large-scale production of dengue recombinant antigens useful for serodiagnosis.
MeSH term(s)	Antibodies, Viral/blood ; Antigens, Viral/genetics ; Chloroplasts/genetics ; Cross Reactions ; Dengue/diagnosis ; Dengue Virus/genetics ; Dengue Virus/immunology ; Epitopes/genetics ; Epitopes/immunology ; Genetic Vectors ; Humans ; Lactuca/genetics ; Molecular Sequence Data ; Plants, Genetically Modified ; RNA, Viral/genetics ; Recombinant Proteins/genetics ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Viral Envelope Proteins/genetics ; Viral Envelope Proteins/immunology
Chemical Substances	Antibodies, Viral ; Antigens, Viral ; Epitopes ; RNA, Viral ; Recombinant Proteins ; Viral Envelope Proteins
Language	English
Publishing date	2013-04-25
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-013-4918-6
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Article ; Online: Analysis of Clinical and Laboratory Alterations Related to Dengue Case Severity: Comparison between Serotypes 2 and 4 in Brazil.

da Costa Faria, Nieli Rodrigues / Solorzano, Victor Edgar Fiestas / de Souza, Luiz José / Nogueira, Rita Maria Ribeiro / de Bruycker-Nogueira, Fernanda / Chouin-Carneiro, Thaís / Santos Simões, Jaqueline Bastos / da Rocha Queiroz Lima, Monique / de Oliveira Pinto, Luzia Maria / Kubelka, Claire Fernandes / Heringer, Manoela / de Azeredo, Elzinandes Leal / Barreto Dos Santos, Flavia

The American journal of tropical medicine and hygiene

2017 Volume 97, Issue 1, Page(s) 137–145

Abstract: The increase in severe dengue (SD) cases has caused great impact on public health and has concerned authorities of countries where the disease is endemic and epidemics reach high proportions. The recognition of progression signs of this severe disease ... ...

Abstract	The increase in severe dengue (SD) cases has caused great impact on public health and has concerned authorities of countries where the disease is endemic and epidemics reach high proportions. The recognition of progression signs of this severe disease during the initial febrile phase can be difficult, since the symptoms are often indistinguishable from other febrile diseases. The aim of this study was to evaluate the clinical manifestations and laboratory findings in patients from two dengue outbreaks and their association with the disease. The study was conducted in patients (
MeSH term(s)	Brazil/epidemiology ; Dengue/epidemiology ; Dengue/pathology ; Dengue/virology ; Dengue Virus/classification ; Dengue Virus/genetics ; Dengue Virus/pathogenicity ; Epidemics ; Humans ; Serogroup
Language	English
Publishing date	2017-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2942-7
ISSN	1476-1645 ; 0002-9637
ISSN (online)	1476-1645
ISSN	0002-9637
DOI	10.4269/ajtmh.16-0227
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Article ; Online: Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection.

Badolato-Corrêa, Jessica / Sánchez-Arcila, Juan Camilo / Alves de Souza, Thiara Manuele / Santos Barbosa, Luciana / Conrado Guerra Nunes, Priscila / da Rocha Queiroz Lima, Monique / Gandini, Mariana / Bispo de Filippis, Ana Maria / Venâncio da Cunha, Rivaldo / Leal de Azeredo, Elzinandes / de-Oliveira-Pinto, Luzia Maria

Immunity, inflammation and disease

2017 Volume 6, Issue 2, Page(s) 194–206

Abstract: Introduction: Zika virus (ZIKV) and dengue virus (DENV) co-circulated during latest outbreaks in Brazil, hence, it is important to evaluate the host cross-reactive immune responses to these viruses. So far, little is known about human T cell responses ... ...

Abstract	Introduction: Zika virus (ZIKV) and dengue virus (DENV) co-circulated during latest outbreaks in Brazil, hence, it is important to evaluate the host cross-reactive immune responses to these viruses. So far, little is known about human T cell responses to ZIKV and no reports detail adaptive immune responses during DENV/ZIKV coinfection. Methods: Here, we studied T cells responses in well-characterized groups of DENV, ZIKV, or DENV/ZIKV infected patients and DENV-exposed healthy donors. We evaluated chemokine receptors expression and single/multifunctional frequencies of IFNγ, TNF, and IL2-producing T cells during these infections. Even without antigenic stimulation, it was possible to detect chemokine receptors and IFNγ, TNF, and IL2-producing T cells from all individuals by flow cytometry. Additionally, PBMCs' IFNγ response to DENV NS1 protein and to polyclonal stimuli was evaluated by ELISPOT. Results: DENV and ZIKV infections and DENV/ZIKV coinfections similarly induced expression of CCR5, CX3CR1, and CXCR3 on CD4 and CD8 T cells. DENV/ZIKV coinfection decreased the ability of CD4 Conclusion: Therefore, we emphasize the potential impact of coinfection on the immune response from human hosts, mainly in areas where DENV and ZIKV cocirculate.
MeSH term(s)	Adaptive Immunity ; Adolescent ; Adult ; Aged ; Brazil/epidemiology ; Coinfection/blood ; Coinfection/epidemiology ; Coinfection/immunology ; Coinfection/virology ; Cross Reactions/immunology ; Dengue/blood ; Dengue/epidemiology ; Dengue/immunology ; Dengue/virology ; Dengue Virus/immunology ; Disease Outbreaks ; Female ; Host-Pathogen Interactions/immunology ; Humans ; Male ; Middle Aged ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; Young Adult ; Zika Virus/immunology ; Zika Virus Infection/blood ; Zika Virus Infection/epidemiology ; Zika Virus Infection/immunology ; Zika Virus Infection/virology
Language	English
Publishing date	2017-12-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2740382-8
ISSN	2050-4527 ; 2050-4527
ISSN (online)	2050-4527
ISSN	2050-4527
DOI	10.1002/iid3.203
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Article: Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses' Outbreak in Rio de Janeiro, Brazil.

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Article: Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil

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Article: Dengue virus tetra-epitope peptide expressed in lettuce chloroplasts for potential use in dengue diagnosis

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Article ; Online: Twenty years of DENV-2 activity in Brazil: molecular characterization and phylogeny of strains isolated from 1990 to 2010.

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Article ; Online: Dengue virus tetra-epitope peptide expressed in lettuce chloroplasts for potential use in dengue diagnosis.

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Article ; Online: Analysis of Clinical and Laboratory Alterations Related to Dengue Case Severity: Comparison between Serotypes 2 and 4 in Brazil.

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Article ; Online: Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection.

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